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研究生:蕭伊芸
研究生(外文):Yi Yun Hsiao
論文名稱:乳羅黛粉葉誘變種之微體繁殖與再誘變
論文名稱(外文):Micropropagagtion and mutation of Dieffenbachia maculata‘Rudolph Roehrs-86-A’
指導教授:朱建鏞
指導教授(外文):Chien Young Chu
學位類別:碩士
校院名稱:國立中興大學
系所名稱:園藝學系
學門:農業科學學門
學類:園藝學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:英文
論文頁數:70
中文關鍵詞:組織培養癒傷組織γ射線誘變葉片培養再分化瓶外發根
外文關鍵詞:leaf culturecallusmutationγ-rayredifferentiaterooting ex vitro
相關次數:
  • 被引用被引用:7
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  • 下載下載:199
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乳羅黛粉葉誘變種以幼嫩完全展開葉為培殖體,培養於1/2 MS配方、20g/l蔗糖、6g/l洋菜與添加BA 5 mg/l及NAA 0.5 mg/l之培養基,可由葉片誘導較多的癒傷組織。將(5mm) 3之癒傷組織塊培養於1/2 MS配方、6g/l洋菜、20g/l蔗糖與添加BA 5 mg/l配合NAA 0.125 mg/l之培養基經5-6週,可再分化植株。植株移出瓶外後,使用枝梢長度在2.5公分以上者,基部以NAA 1g/kg粉劑處理,發根率最佳。
Shoot regeneration through callus culture was induced successfully from young expanded leaves of Dieffenbachia maculata ‘Rudolph Roehrs-86M1’in vitro. Young leaves were cut into 1 ×1cm pieces and surface sterilized in 0.5% sodium hypochlorite for 10 min followed by washing with sterile distilled water. The contamination was controlled under 25%. Leaves were cultured on the medium containing 1/2 MS, sugar 20g/l, agar 6g/l, BA 5 mg/l and NAA 0.5 mg/l and were induced more callus .
Shoot proliferated when callus explant of 5mm in cubic was cultured on the medium containing 1/2 MS, agar 6g/l, sugar 20g/l, BA 5 mg/l and NAA 0.125 mg/l. Shoot longer than 2.5 cm rooted well when shoot base was coated with NAA 0.1%.
There was 50% or 66.7% of callus proliferating shoot, when callus of 5mm×5mm was irradiated 4 Gy γ-ray once or twice, respectively. However, callus of 10mm×10mm was irradiated once, the rate of shoot proliferation increased to 76.8%. And there was 50% of callus proliferating shoot when irradiated twice.
壹、前言
貳、前人研究
參、材料與方法
肆、結果
伍、討論
陸、摘要
柒、參考文獻
柒、參考文獻
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