跳到主要內容

臺灣博碩士論文加值系統

(54.92.164.9) 您好!臺灣時間:2022/01/23 05:28
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果 :::

詳目顯示

我願授權國圖
: 
twitterline
研究生:蔡惠文
研究生(外文):Hui-Wen Tsai
論文名稱:柿樹嫁接不親和性及耐寒性之研究
論文名稱(外文):Studies on the Graft Incompatibility and Cold Hardiness of Persimmon Tree (Diospyros Kaki L.)
指導教授:林慧玲林慧玲引用關係
指導教授(外文):Huey-Ling Lin
學位類別:碩士
校院名稱:國立中興大學
系所名稱:園藝學系
學門:農業科學學門
學類:園藝學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:中文
論文頁數:98
中文關鍵詞:嫁接不親和性樹皮萃出液過氧化酵素
外文關鍵詞:persimmongraft incompatibilitybark extractsperoxidase
相關次數:
  • 被引用被引用:5
  • 點閱點閱:1345
  • 評分評分:
  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:0
本試驗目的為探討‘富有’可能因產生某些毒質,藉由韌皮部的運輸,傳送到山豆柿的根部,而使山豆柿的根系發育受損,進而影響養分、水分的吸收與運移而產生嫁接不親和性之障礙。
不同穗砧組合,以山豆柿/山豆柿的植株生長較快,嫁接處的癒合較佳,‘富有’/山豆柿的植株生長較慢。在碳水化合物的累積,‘富有’/山豆柿地上部的全可溶性糖較少,但澱粉含量多。莖的總酚類化合物含量,以‘富有’/山豆柿高於山豆柿/山豆柿的穗砧組合。
以1%、5%、10%之山豆柿、‘富有’、‘牛心柿’樹皮萃出液及去離子水為對照組,處理山豆柿種子。樹皮液處理組,會抑制山豆柿幼苗根系的生長,以10%‘富有’樹皮萃出液的抑制較明顯,其根尖細胞褐化、皺縮,影響水分運移。山豆柿根系的可溶性單寧、總酚類化合物及全可溶性蛋白質含量均以10%‘富有’樹皮液處理組最高,推測是‘富有’樹皮液中含有某種酚類物質,使山豆柿根系生長受抑制。
‘富有’樹皮萃出液,濃度1%、10%對山豆柿種子發芽率上無顯著差異。植株發育上則表現顯著抑制生長現象。山豆柿植株鮮重與乾重均以對照組較高,而總酚類化合物以1%與10%處理組較高。
過氧化酵素活性與電泳分析,因品種的差異而有不同的酵素活性與條帶,初步結果顯示過氧化酵素的活性、電泳圖譜與柿樹嫁接親和性沒有明顯的相關性,仍待進一步研究。
山豆柿、‘平核無’、‘牛心柿’、‘富有’枝條分別於落葉後及萌芽前取樣,經-22.5℃處理後的離子滲漏值,以山豆柿的值最高,澱粉含量最低。‘富有’離子滲漏值最低,澱粉含量最高,顯示耐寒性與澱粉累積有關。萌芽前取樣,以‘平核無’與‘富有’的離子滲漏值最低。全可溶性糖含量,以‘平核無’與‘富有’的含量最高,此時的耐寒性與全可溶性糖含量有關。
The objective of this experiment was to investigate the graft incompatibility between ‘Fuyu’ and D. japonica. It is general believed that the ‘Fuyu’ persimmon could produce certain toxic chemical compound which can be transported via phloem to the root of D. japonica. The toxic principle is harmful to the development of the root system of D. japonica during graftage by affecting mineral nutrients uptake and translocation.
Among the various combination of scions and rootstocks, the compatibility between D. japonica scion grafted on the same rootstock performed relatively better than ‘Fuyu’ grafted onto D. japonica. The former had rapid growth rate, more carbohydrate and cells fusion quicker at the grafting union. In contrast, the latter combination had a lower growth rate, more starch and less carbohydrate in the scion leaves. Total phenolic compounds content of Fuyu/D. japonica were higher than D. japonica/D. japonica in the stem.
Tissue samples (1%, 5%, 10 % barks) of D. japonica, ‘Fuyu’, ‘Bull Heart’ were extracted with deionized water (use water as a check). Bark extracts were used to treat the seeds of D. japonica. The root growth of the seedling was slightly inhibited by 1% of bark extract and was significantly inhibited at 10 %. The affected cells were brown in color and shrunken at the root apex. The symptom seemed to show that water transport was affected. Chemical analysis showed that the soluble tannin, total phenolic compounds and soluble protein content were high in the seedling root with 10 % Fuyu bark extract treatment. It is presumed that Fuyu bark extract contain certain kind of phenolic compound which was responsible for the inhibition of seedling root growth.
Experiment data indicated that no difference was found between 1% and 10% Fuyu bark extract on seeds germination, but growth of seedlings of D. japonica were significantly inhibited. Dry and fresh weights of D. japonica were less than the control while total phenolic compound was higher after 1% or 10% bark extracts treatment.
Results of peroxidase activity and electrophoresis showed different enzyme activities and band patterns due to the dissimilarity in varieties. Primary results disclosed that there was no significant relationship between peroxidase isozyme pattern and graft compatibility.
D.japonica, ‘Hiratanaenashi, ‘Bull Heart’, ‘Fuyu’ persimmon shoots were collected separately at leaf abscission and pre-bud bursting stage. Electrolyte leakages from the deep-freezed (-22.5℃) tissues were determined. Results of leaf abscission indicated that D. japonica had the highest leakage level but the lowest starch content while Fuyu had the lowest leakage but with the highest starch. This seemed to indicate a positive relation between cold hardness and starch content. Samples before bud bursting showed that ‘Hiratanenashi’ and ‘Fuyu’ had the lowest electrolyte leakage and highest soluble sugar content also indicated a positive relationship between the cold hardness and soluble carbohydrate.
目 錄
壹、前言………………………………………………………… 1
貳、前人研究…………………………………………………… 4
一、柿之概說……………………………………………… 4
二、嫁接不親和性之探討………………………………… 7
三、嫁接不親和性毒質之探討…………………………… 12
四、同功異構酵素與嫁接親和性之關係………………… 15
五、碳水化合物及酚類化合物與耐寒性之關係………… 19
參、材料與方法………………………………………………… 21
肆、結果………………………………………………………… 31
一、不同穗砧組合柿樹之生長與發育…………………… 31
二、不同柿樹品種及濃度之樹皮萃出液對山豆柿幼苗根系發育之影響…………………………………… 33
三、‘富有’樹皮萃出液對山豆柿種子發芽率及植株發育之影響…………………………………………… 39
四、不同品種之接穗過氧化酵素活性及電泳之分析…… 41
五、柿樹枝條耐寒性試驗………………………………… 44
伍、討論………………………………………………………… 76
一、柿樹穗砧之不親和性………………………………… 76
二、柿樹枝條耐寒性試驗………………………………… 85
中文摘要………………………………………………………… 87
英文摘要………………………………………………………… 88
參考文獻………………………………………………………… 90
附錄1…………………………………………………………… 98
王元裕、李伯均、周碧英、柳國華、鄭顯明。 1996。甜柿砧穗組合嫁接親和力研究。園藝學報 23: 110-114。
王昭月。1995。同功異構酵素與逢機增殖多型性DNA標誌在番椒品種鑑別之研究。中興大學園藝系碩士論文。pp.121-123
王勝鴻。 1978。接木不親和性。科學農業 26: 241-246。
朱德民。1995。植物與環境逆境。明文書局 pp.309-339。
周昌弘。1992。植物生態學。pp.252-265。聯經出版有限公司。台灣,台北。
林榮貴。1993。台灣目前柿子栽培問題。興農 228: 48-51。
林榮貴。1996。日本甜柿的重要栽培品種。農業世界 159: 22-28。
林榮貴。 2001a。柿的栽培-參.砧木的選擇與嫁接親和性。農業世界 211:81-83。
林榮貴。2001b。 台灣的柿品種. 種苗科技專訊 33: 6-11。
林嘉興。2000。台灣甜柿產業發展及產地分佈。甜柿栽培與管理技術。台中區農業改良場特刊 46: 1-3。
林嘉興、張致盛。2000。柿子栽培管理技術。甜柿栽培與管理技術。台中區農業改良場特刊 46: 13-25。
郭純德、蔡疇、張永欣。1994。台灣柿產業之現況及展望。農政與農情 9: 48-52。
范念慈。1985。葡萄柚根砧自身毒害生理研究。興大園藝 10: 1-4。
倪正柱、林文彬。日本柿(Diospyros kaki Thunb)培育及穗砧不親和性調查。1994。興大園藝 19: 61-69。
張致盛。2000。台灣栽培柿子品種介紹。甜柿栽培與管理技術。台中區農業改良場特刊 46: 5-9。
陳玉雯。2001。芹菜連作障礙之研究。中興大學園藝系碩士論文。
溫英杰。1995。柿品種評估暨改良。 中華農業研究 44: 49-58。
劉雲聰 。2000。富有柿嫁接及果實生長之研究。中興大學園藝系碩士論文。122 pp.
蔡巨才。柿嫁接不親和性對生長之影響。1998。中國園藝 44: 11-28。
Andrews, P.K. and C.S. Marquez. 1993. Graft incompatibility. Hort. Rev. 15: 183-232.
Arulsekar, S. and D.E. Parfitt. 1986. Isozyme analysis procedures for stone fruits, almond, grape, walnut, pistachio, and fig. HortScience 21: 928-933.
Bachmann, M., P. Matile, and F. Keller. 1994. Metabolism of the raffinose family oligosaccharides in leaves of Ajuga reptans L. Plant Physiol. 105: 1335-1345.
Barkosky, R.R. and F.A. Einhellig. 1993. Effect of salicylic acid on plant-water relationships. J. Chem. Ecol. 19: 237-247.
Bergmark, C.L., W.A. Jackson, R.J. Volk, and U. Blum. 1992. Differential inhibition by ferulic acid of nitrate and ammonium uptake in Zea mays L. Plant Physiol. 98: 639-645.
Blum, U. and S.R. Shafer. 1988. Microbial populations and phenolic acids in soil. Soil Biol. Biochem. 20: 793-800.
Blum, U. and J. Rebbeck. 1989. Inhibition and recovery of cucumber roots given multiple treatments of ferulic acid in nutrient culture. J. Chem. Ecol. 15: 917-928.
Booker, F.L., U. Blum and E.L. Fiscus. 1992. Short-term effects of ferulic acid on ion uptake and water relations in cucumber seedlings. J. Exp. Bot. 93: 649-655.
Brown, R.L., C.S. Tang, and R.K. Nishimoto. 1983. Growth inhibition from guava root exudates. HortScience 18: 316-318.
Burger, W.P. and J.G.C. Small. 1983. Allelopathy in citrus orchards. Sci Hort. 20: 361-375.
Castonguay, Y., P. Nadeau, P. Lechasseur, and L. Chouinard. 1995. Differential accumulation of carbohydrates in alfalfa cultivars of contrasting winter hardiness. Crop Sci. 35: 509-516.
Cameron, S.H. 1932. Starch in the young orange tree. Proc. Amer. Soc. Hort. Sci. 29: 110-114.
Devi, S.R. and M.N.V. Prasad. 1992. Effect of ferulic acid on growth and hydrolytic enzyme activities of germinating maize seeds. J. Chem. Ecol. 18: 1981-1990.
Dey, P.M. and J.B. Harborne. Phenolic Metabolism. Plant Biochemistry.pp.387-416. Academic Press, Inc.
Dubois, M. 1956. Colormetric method for determination of sugar and related substance. Anal. Chem. 28: 350-356.
Einhellig, F.A. and J.A. Rasmussen. 1993. Effect of root exudates sorgoleone on photosynthesis. J. Chem. Ecol. 19: 369-375.
Elstner, E.F., W. Obwald, R. Volpert and H. Schempp. 1994. Phenolic antioxidants. Acta Hort. 381: 301-335.
Errea, P. 1998. Implications of phenolic compounds in graft incompatibility in fruit tree species. Sci. Hort. 74: 195-205.
Feucht,W., P.P.S. Schmid, and E. Christ. 1985. Determination of peroxidase and acid phosphatases in the phloem of cherry grafts by microscopic photometry. Acta Hort. 169: 179-186.
Feucht,W., and P.P.S. Schmid. 1988. Incompatibility of grafted cherry trees (P. avium / P. cerasus) : loss of metabolic tolerance as a consequence of environmental stress. Acta Hort. 227: 87-89.
Feucht, W. 1988. Graft incompatibility of tree crops: An overview of the present scientific status. Acta Hort. 277: 33-41.
Feucht,W. 1994. The localization of phenols at the cellular and tissue level. Acta Hort. 381: 803-815.
Flinn, C.L., and E.N. Ashworth. 1995. The relationship between carbohydrates and flower bud hardiness among three Forsythia taxa. J. Am. Soc. Hort. Sci. 120: 607-613.
Garner. 1979. Compatibility and cambial contact. p. 49-67. In: The Grafter’s Handbook.4th ed. Oxford University Press, New York.
Gebhardt, K. and W. Feucht. 1982. Polyphenol changes at the union of prunus avium / Prunus cerasus grafts. J. Hort. Sci. 57: 253-258.
Glass, A.D.M. and B.A. Bohm. 1971. The uptake of simple phenols by barley roots. Planta 100: 93-105.
Gulen, H., R. Arora, A. Kuden, S.L. Krebs, and J. Postman. 2002. Peroxidase isozyme profiles in compatibile and incompatible pear-quince graft combinations. J. Amer. Soc. Hort. Sci. 127: 152-157.
Gur, A. 1957. The compatibility of the pear with quince rootstock. Spec. Bul. 10, Agr. Res. Sta. Rehovot(Israel). p.1-9
Hartmann, H.T., D.E. Kester, F.T. Davies, and R.L. Geneve. 1997. Plant Propagation: Principles and Practices. The biology of grafting. pp. 395. Prentice-Hall, Upper Saddle River, N. J.
Hazebroek, J.P., S.A. Garrison, and T. Gianfagna. 1989. Alleopathic substances in asparagus roots: extraction, characterization, and biological activity. J. Amer. Soc. Hort. Sci. 114: 152-158.
Hejl, A.M., F.A. Einhellig and J.A. Rasmussen. 1993. Effect of juglone on growth, photosynthesis and respiration. J. Chem. Ecol. 19: 559-568.
Huang, H., J.D. Norton, G.E. Boyhan, and B.R. Abrahams. 1994. Graft compatibility among chestnut ( Castanea ) species. J. Amer. Soc. Hort. Sci. 119: 1127-1132.
Inderjit and K.M.M. Dakshini. 1995. On laboratory bioassays in allelopathy. Bot. Rev. 61: 28-44.
Inderjit. 1996. Plant phenolics in allelopathy. Bot. Rev. 62: 186-202.
Inderjit and M. Muramatsu. 1997. On the allelopathic potential of certain terpenoids, phenolics, and their mixtures, and their recovery from soil. Can. J. Bot. 75: 888-891.
Itamura, H., Leng, P. and Yamamura, H.,1994. Effects of some chemicals on breaking bud dormancy in Japanese persimmons. Environ. Cont. in Biology. 32: 171-176.
Jankay, P. and W.H. Muller. 1976. The relationships among umbellifeone, growth and peroxidase levels in cucumber roots. Amer. J. Bot. 63: 126-132.
Jeffree, C.E., and M.M. Yeoman. 1983. Development of intercellular connections between opposing cells in a graft union. New Phytol. 93: 491-509.
Johnson, L.B., and B.A. Cunningham. 1972. Peroxidase activity in healthy and leaf—rust-infected wheat leaves. Phytochemistry. 1: 547-551.
Keith, R.W., D.L. Tourneau, and D. Mahlum. 1958. Quantitative chromatographic determination of phenols. J. Chromatography. 1: 534-536.
Koster, K.L. and D.V. Lynch. 1992. Solute accumulation and compartmentation during the cold acclimation of Puma Rye. Plant Physiol. 98: 108-113.
Lee, C.Y., and N.L. Smith. 1979. Blanching effect on polyphenoloxidase activity in table beets. J. Food Sci. 44: 82-86.
Leng, P., H. Itamura, and H. Yamamura. 1993. Freezing tolerance of several Diospyros species and kaki cultivars as related to anthocyanin formation. J. Japan. Soc. Hort. Sci. 61: 795-804.
Leng, P., T. Okamori, H. Itamura, and H. Yamamura. 1997. Development of frost hardiness in Japanese persimmons. Acta Hort. 436: 109-118.
Liu, Y., X. Dexing, L. Shanjun, L. Laishui, and Y. Fanghong. 1998. The anatomical feasture observation of ‘Fuyu’ persimmon grafted with some rootstocks. Acata Agriculturae Universitatis Jiangxiensis. 20: 394-397.
Lockhard, R.G. and G.W. Schneider. 1981. Stock and scion growth relationships and the dwarfing mechanism in apple. Hort. Rew. 3: 315-375.
Lowry, O.H., N.J. Roseborough, A.L. Farr, and R.J. Rondall. 1951. Protein measurement with the Folin-Phenol reagent. J. Biol. Chem. 193: 265-275.
Luckwill, L.C. and C.V. Cutting. 1970. Physiology of tree crops pp.120-142. Academic Press, London and New York.
Lyu, S.W., U. Blum, T.M. Gerig, and T.E. O’brien. 1990. Effects of mixtures of phenolic acids on phosphorus take by cucumber seedling. J. Chem. Ecol. 16: 2559-2568.
Margna, U. 1977. Control at the level of substrate supply- an alternative in the regulation of phenylpropanoid accumulation in plant cells. Phytochemistry 16: 419-42.
Moore, R. 1983. Physiological aspects of graft formation, p. 89-105. In: R. Moore, (ed.). Vegetative compatibility responses in plants, Baylor Univ. Press, Waco, Texas.
Moore, R. 1984. A model for graft compatibility-incompatibility in higher plants. Amer. J. Bot. 71: 752-758.
Moore, R. 1986. Graft incompatibility between pear and quince : the influence of metabolites of cydonia oblonga on suspension cultures of pyrus communis. Amer. J. Bot. 73: 1-4.
Mosse, B. 1962. Graft incompatibility in fruit trees. Tech. Comm. 28. Comm. Bur. Hort. Plant Crops, East Malling, England.
Palta, J.P. and P.H. Li. 1978. Examination of ultrastructural freeze-injury in the leaf cells of tender and hardy potato species. HortScience 13: 387.
Palonen, P. 1999. Relationship of seasonal changes in carbohydrates and cold hardiness in canes and buds of three red raspberry cultivars. J. Amer. Soc. Hort. Sci. 124: 507-513.
Parfitt, D. E. and S. Arulsekar. 1989. Inheritance and isozyme diversity for GPI and PGM among grape cultivars. J. Amer.Soc. Hort. Sci. 114: 486-491.
Peirce, L.C., and J.L. Brewbaker. 1973. Applications of isozyme analysis in horticultural science. HortScience. 8: 17-22.
Rhodes, D., S. Handa and R.A. Bressan. 1986. Metabolic changes associated with adaptation of plant cells to water stress. Plant Physiol. 82: 890-903.
Ramirez, H., A. Hussain, W. Roca and W. Bushuk. 1987. Isozyme electrophoregrams of sixteen enzyme in five tissue of cassava (Manihot esculenta Crantz) varieties. Euphytica 36: 39-48.
Rice, E. L. 1984. Allelopathy. Academic Press. Orlando, FL.
Sachs, T. 1981. The control of the patterned differentiation of vascular tissues . Adv. Bot. Res. 9: 151-262.
Salisbury, F.B., and C.W. Ross. 1992. Plant Physiology, 4th ed. Wadsworth Publishing, Belmont, CA, 619 pp.
Santamour, F.S.Jr. 1988a. Graft incompatibility related to cambial peroxidase isozymes in Chinese chestnut. J. Environ. Hort. 6: 33-39.
Santamour, F.S.Jr. 1988b. Graft peroxidase enzymes related to graft incompatibility in red oak. J. Environ. Hort. 6: 87-93.
Scandalios, J.G. 1974. Isozyme in development and differentiation. Ann. Rev. Plant Physiol. 25: 225-258.
Schmid, P.P.S., and W. Feucht. 1985. Compatibility in Prunus avium / Prunus cerasus graftings during the initial phase. III. Isoelectrofocusing of proteins, peroxidase and acid phosphatases during union formation. Journal of Horticultural Science 60: 311-318.
Shilling, D.G., J.A. Dusky, M.A. Mossler, and T.A. Bewick. 1992. Allelopathic potential of celery residues on lettucee. J. Amer. Soc. Hort. Sci. 117: 308-312.
Stebbins, G.L. 1989. Introduction pp.1-4. In: Solitis, D.E. and P.S. Soltis(eds.). Isozymes in Plant Biology. Dioscorides, Oregon.
Taira, S. 1996. Astringency in persimmon. pp.97-110. In: Linskens, H. F. and J. F. Jackson. Fruit analysis. Springer-Verlag Berlin Heidelberg, Inc, Germany.
Taiz, L. and E. Zeiger. 1991. Plant Physiology. The Benjamin / Cumming Publishing Company, Inc. California. pp. 320-321.
Wang, R., Yangyong and G. Li. 1997. Research on cold hardiness of germplasm resources of persimmon (Diospyros kaki). Acta Hort. 436: 101-107.
Wardale, D.A. 1973. Effect of phenolic compounds in Lycopersicum esculentum on the synthesis of ethylene. Phytochemistry 12: 1523.
Welinder, K.G. 1991. The plant peroxidase superfamity. In: Lobarzewski, J., H. Greppin, C. Penel, and T. Gaspar(Eds). Biochemical, physiological and molecular aspects of plant peroxidase. Geneva: University of Geneva press pp.3-14.
Wendel, J.F. 1989. Visualization and interpretation of plant isozyme. pp.5-45. In : D.E. Soltis and P. S. Soltis(eds.) Isozymes in plant biology. Dioscorides, Oregon.
Yu, K. and R.F. Carlson. 1975. Gas liquid chromatography determinations of phenolic acids and coumarins in mazzard and mahaleb cherry seedlings. Horticultural Science 10: 401-403.
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top