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研究生:王暉景
研究生(外文):Huei-Jing Wang
論文名稱:百合花粉LLA23蛋白質的核定位訊息序列之功能分析
論文名稱(外文):Funtional Analysis of Nuclear localization Signal of the LLA23 Protein in Lily Pollen
指導教授:王國祥
指導教授(外文):Co-Shine Wang
學位類別:碩士
校院名稱:國立中興大學
系所名稱:農業生物科技學研究所
學門:農業科學學門
學類:農業技術學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:中文
論文頁數:74
中文關鍵詞:核定位訊息序列LLA23蛋白質
外文關鍵詞:Nuclear Localization SignalLLA23 Protein
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LLA23為百合花藥 (Lilium longiflorum anther) 成熟晚期表現的花粉專一性基因。LLA23 mRNA轉錄一含142個胺基酸的蛋白質,其C端有一bipartite NLS (nuclear localization signal) 序列。此核定位訊息序列A區有四個離胺酸,B區有二個離胺酸,中間相隔了7個其它不同的胺基酸。為了探討LLA23蛋白質核定位訊息序列的功能,取其C半端的LLA23 cDNA序列接上綠色螢光蛋白質的報導基因,利用竹嵌紋病毒 (Bamboo mosaic potexvirus) 載體在白藜 (Chenopodium quinoa) 葉面上表現外源蛋白質。在感染八天的白藜葉片上可以看到明顯的病斑。利用32P-dCTP標定的綠色螢光蛋白質基因當探針進行北方墨點分析,可在白藜葉內偵測到病毒的重組基因體和兩個重組次基因體RNA。以抗綠色螢光蛋白質的抗體進行西方墨點法檢測,可在白藜葉內檢測出綠色螢光蛋白質。在螢光顯微鏡和雷射共軛焦掃描顯微鏡觀察下,白藜葉片病斑周邊的細胞有聚集成點狀的綠色螢光。DAPI (4'-6-diamidino-2-phenylindole) 染色的結果顯示,點狀的綠色螢光就是聚集在細胞核,而去除了核定位訊息序列的GFP-ΔLLA23融合蛋白質的螢光則散佈於細胞質中,證明LLA23的核定位訊息序列可以把綠色螢光蛋白質帶進核內。將LLA 23蛋白質的核定位訊息序列 A區和B區作定點突變,使離胺酸突變成丙胺酸。突變掉A區四個離胺酸的融合蛋白質進核效率為23 %,而突變掉B區二個離胺酸為57 %,顯示出核定位訊息序列 A區把螢光蛋白質帶進核的效率比B區大。核定位訊息序列 A區四個離胺基若分別突變掉第一、二個離胺酸會顯著的降低融合蛋白質進核的效率,分別為33 %和42 %。假若同時突變掉此二個離胺酸,融合蛋白質的進核效率為31 %,顯示A區的第一、二個離胺酸同等重要,缺一不可。若分別突變A區的第三、四個離胺酸,融合蛋白質進核的效率為幾乎不變。若同時突變掉A區此二個離胺酸,融合蛋白質進核的效率仍為94 %,顯示A區第三、四個離胺酸對融合蛋白質的進核效率沒有影響。總言之,LLA 23蛋白質的核定位訊息序列有入核的功能,其核定位訊息序列 A區的前兩個離胺酸與B區的離胺酸都會影響融合蛋白質入核的功能,而A區的影響顯然較B區重要。本實驗的結果間接証明了LLA 23蛋白質可以進入細胞核。

LLA (Lilium longiflorum anther) 23 expressed at the stage of pollen maturation is a pollen-specific gene. The LLA23 mRNA encodes a protein containing 142 amino acids in which the bipartite nuclear localization signal (NLS) is located at the C-terminus of the polypeptide. The NLS sequence of LLA23 is composed of two regions rich in lysine residues. The A region of NLS contains four lysine residues and the B region of NLS two lysine residues. A segment of seven amino acids other than lysine resides between the two regions of NLS. To study the functional NLS of LLA23 protein, the C-terminal half of LLA23 cDNA sequence was fused with the coding region of a green fluorescence protein (GFP) as a reporter and constructed into bamboo mosaic potexvirus (BaMV) to facilitate infection on leaves of Chenopodium quinoa. After 8 days of infection by BaMV, local lesions on the leaf were clearly observed. Northern blots analyses demonstrated that a recombinant genomic and two recombinant subgenomic RNAs were detected when 32P-dCTP labeled GFP coding region was used as a probe. Immunoblots of leaf protein confirmed that the GFP was detected using anti-GFP antiserum. Under either fluorescence microscope or laser confocal scanning microscopy, the green fluorescence were condensed into spots co-localized with nuclei of the mesophell cells as demonstrated by DAPI (4′-6-diamidino-2-phenylindole) staining. A deletion of the NLS from GFP-ΔLLA23 resulted in the distribution of the green fluorescence in the cytoplasm. It strongly suggested that the NLS of LLA23 has a function of importing GFP into nucleus. Mutagenesis was done either on A or B regions of the NLS by substitution of lysine residue into alanine. Mutagenesis of four lysines together in the A region resulted in 23 % efficiency of locating the fusion protein in the nucleus whereas mutagenesis of the two lysines together in the B region resulted in 57 % importing efficiency. It indicated that A region has more profound influence on nuclear importing than B region. Mutations in the first two lysines in the A region either together or separately reduced to 31-42 % of importing efficiency while no significance of nuclear import was observed when the third and the fourth lysines of A region were mutated either together or separately. In all, the LLA23 NLS has a function of nuclear importing. Both the first two lysine residues at A region and the two lysine residues at B region have the influence on nuclear location. However, the A region has more significant efficiency of importing than that of B region. The data herein demonstrate that LLA23 protein can import into nucleus of the cell.

壹、 中文摘要----------------------------------------------1
貳、 英文摘要----------------------------------------------3
參、 文獻整理----------------------------------------------5
一、 百合--------------------------------------------------5
二、 花藥之發育--------------------------------------------5
三、 與乾燥有關之蛋白和基因之研究--------------------------8
四、 蛋白質進入細胞核機制之相關研究-----------------------11
肆、 材料與方法-------------------------------------------18
一、 GFP-LLA23和GFP-∆LLA23兩融合蛋白質基因之構築----------18
二、 各組融合蛋白質DNA構築體的選殖------------------------19
三、 GFP-LLA23之生體外點突變------------------------------22
四、 各組重組竹嵌紋病毒載體的構築-------------------------23
五、 各組重組竹嵌紋病毒RNA的製備與接種--------------------23
六、 北方墨點法-------------------------------------------24
七、 西方墨點法-------------------------------------------27
八、 顯微鏡觀察-------------------------------------------30
九、 雷射共軛焦掃描顯微鏡數位照片之數據分析---------------30
伍、結果------------------------------------------------------31
一、 GFP-LLA23和GFP-∆LLA23融合蛋白質之構築----------------31
二、 LLA23蛋白質的核定位訊息序列為進核的訊息序列----------31
三、 LLA23蛋白質核定位訊息序列 A與B區的定點突變-----------33
四、 GFP-LLA23蛋白質核定位訊息序列 A區的定點突變----------34
五、 雷射共軛焦掃描顯微鏡觀察結果的螢光量化---------------34
陸、討論------------------------------------------------------37
柒、參考文獻--------------------------------------------------43
捌、圖表------------------------------------------------------55
玖、附圖------------------------------------------------------74

胡適宜。1991。被子植物之胚胎學,曉園出版社,25-33頁。
蔡月夏。1995。台灣農家要覽農作篇(二),財團法人豐年社主編,585-588頁。
Ach, R. A. and Gruissem, W. (1994) A small GTP-binding protein from tomato suppresses a Schizosaccharomyces prombe cell-cycle mutant. Proc. Natl. Acad. Sci. USA 91:5863-5867.
Allen, T. D., Cronshaw, J. M., Bagley, S., Kiseleva, E., and Goldberg, M. W. (2000) The nuclear pore complex: mediator of translocation between nucleus and cytoplasm. J. Cell Sci. 113:1651-1659.
Astwood, J. D. and Hill, R. D. (1996) Cloning and expression pattern of Hor v 9, the group 9 pollen isoallergen from barley. Gene 182: 53-62.
Ballas, N. and Citovsky, V. (1997) Nuclear localization signal binding protein from Arabidopsis mediates nuclear import of Agrobacterium VirD2 protein. Proc. Natl. Acad. Sci. USA 94:10723-10728.
Barik, S. (1997) Mutagenesis and gene fusion by megaprimer PCR. Methods Mol. Biol. 67:173-82.
Bassel, G., and Singer, R. H. (1997) mRNA and cytoskeletal filaments. Curr. Opin. Cell Biol. 9: 109-115.
Bayliss, R., Littlewood, T., and Stewart, M. (2000) Structural basis for the interaction between FxFG nucleoporin repeats and importin-β in nuclear trafficking. Cell 102:99-108.
Bedinger, P. A., Kristine, J. H., and Cynthia, A. L. (1994) Travelling in style: the cell biology of pollen. Trends Cell Biol. 4: 132-138.
Bischoff, F. R., and Ponsting, H. (1991) Catalysis of guanine nucleotide exchange on Ran by the mitotic regulator RCC1. Nature 354: 80-82.
Blometedt, C. K., Knox, R. B., and Singh, M. B. (1996) Generative cells of Lilium longiflorum possess translatable mRNA and functional protein synthesis machinery. Plant Mol. Biol. 31: 1083-1086.
Borg, S., Brandstrup, B., Jenson, T. J., and Poulsen, C. (1997) Identification of new protein species among 33 different small GTP-binding proteins encoded by cDNAs from Lotus japonicus, and expression of corresponding mRNAs in developing root nodules. Plant J. 11: 237-250.
Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 284-282.
Burnette, W. N. (1981) “Western blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radioiodinated protein A. Anal. Biochem. 112: 195-203.
Canel, C., Bailey-Serres, J. N., and Roose, M. L. (1995) Pummelo fruit transcript homologous to ripening-induced genes. Plant Physiol. 108: 1323-1324.
Chang, S., Puryear, J. D., Dias, M. A. D. L., Funkhouser, E. A., Newton, R. J., and Cairney, J. (1996) Gene expression under water deficit in loblolly pine: isolation and characterization of cDNA clones. Physiol. Plant. 97: 139-148.
Choi, I. R., Stenger, D. C., Morris, T. J., and French, R. (2000) A plant virus vector for systemic expression of foreign genes in cereals. Plant J. 23: 547-555.
Citovsky, V., Zupan, J., Warnick, D., and Zambryski, P. (1992) Nuclear localization of Agrobacterium VirE2 protein in plant cells. Scince 256: 1802-1805.
Close, T. J., Fenton, R. D., and Moonan, F. (1993) A view of plant dehydrins using antibodies specific to the carboxy terminal peptide. Plant Mol. Biol. 23: 279-286.
Close, T. J., Kortt, A. A., and Chandle, P. M. (1989) A cDNA-based comparison of dehydration-induced proteins (dehydrins) in barley and corn. Plant Mol. Biol. 13: 95-108.
Cokol, M., Nair, R., and Rost, B. (2000) Finding nuclear localization signals. EMBO Reports 1: 411-415.
Doye, V., and Hurt, E. (1997) From nucleoporin to nuclear pore complexes. Curr. Opin. Cell Biol. 9:401-411.
Dure, L. III., Crouch, M., Harada, J., Ho, T. H. P., Mundy, J., Quatrano, R., Thomas, T., and Sung, R. Z. (1989) Common amino acid sequence domain among Lea proteins of higher plants. Plant Mol. Biol. 12: 475-486.
Dure, L. III. (1993) A repeating 11-mer amino acid motif and plant desiccation. Plant J. 3: 363-369.
Eady, C., Lindsey, K., and Twell, D. (1994) Differential activation and conserved vegetative cell-specific activity of a late pollen promoter in species with bicelluar and tricellular pollen. Plant J. 5: 543-550.
Eady, C., Lindsey, K., and Twell, D. (1995) The significance of microspore division symmetry for vegetative cell-specific transcription and generative cell differentiation. Plant Cell 7: 65-74.
Engelmeier, L., Olivio, J.-C., and Mattaj, I. W. (1999) Receptor-mediated substrate translocation through the nuclear pore complex without nucleotide triphosphate hydrolysis. Current Biol. 9: 30-41.
Espelund, M., Saeboe-Larssen, S., Hughes, D. W., Galau, G. A., Larsen, F., and Jakobsen, K. S. (1992) Late embryogenesis abundant genes encoding proteins with different numbers of hydrophilic repeats are regulated differentially by abscisic acid and osmotic stress. Plant J. 2: 241-252.
Fu, X. D. (1995) The superfamily of arginine/serine-rich splicing factors. RNA 1:663-680.
Fu, X. D., Mayeda, A., Maniatis, T., and Krainer, A. R. (1992) General splicing factors SF2 and SC35 have equivalent activities in vitro, and both affect alternative 5′ and 3′ splice site selection. Proc. Natl. Acad. Sci. USA 89:11224-11228.
Ge, H., and Manley, J. L. (1990) A protein factor, ASF, controls cell-specific alternative splicing of SV40 early pre-mRNA in vitro. Cell 62: 25-34.
Ge, H., Zuo, P., and Manley, J. L. (1991) Primary structure of the human splicing factor ASF reveals similarities with Drosophila regulators. Cell 66:373-382.
Görlich, D. (1997) Nuclear protein import. Curr. Opin. Cell Biol. 9: 412-419.
Görlich, D., Dabrowski, M., Bischoff, F. R., Kutay, U., Bork, P., Hartmann, E., Prehn, S., and Izaurralde, E. (1997) A novel class of RanGTP binding proteins. J. Cell Biol. 138: 65-80.
Görlich, D., Henklein, P., Laskey, R. A., and Hartmann, E. (1996a) A 41 amino acid motif in importin-alpha confers binding to importin-beta and hence transit into the nucleus. EMBO J. 15: 1810-1817.
Görlich, D., Kostka, S., Kraft, R., Dingwall, C., Laskey, R. A., Hartmann, E., and Prehn, S. (1995) Two different subunits of importin cooperate to recognize nulcear localization signals and bind them to the nuclear envelope. Curr. Biol. 5: 383-392.
Görlich, D., Pante, N., Kutay, U., Aebi, U., and Bischoff, F. R. (1996b) Identification of different roles for RanGDP and RanGTP in nuclear protein import. EMBO J. 15: 5584-5594.
Görlich, D., Prehn, S., Laskey, R. A., and Hartmann, E. (1994) Isolation of a protein that is essential for the first step of nuclear protein import. Cell 79: 767-778.
Greber, U. F., Suomalainen, M., Stidwill, R. P., Boucke, K., Ebersold, W., and Helenius, A. (1997) The role of the nuclear pore complex in adenovirus DNA entry. EMBO J. 16: 5998-6007.
Gross, T., Richert, K., Mierke, C., Lutzelberger, M., and Kaufer, N. F. (1998) Identification and characterization of srp1, a gene of fission yeast encoding a RNA binding domain and a RS domain typical of SR splicing factors. Nucl. Acids Res. 26: 505-511.
Gui, J. F., Lane, W. S., and Fu, X. D. (1994) A serine kinase regulates intracellular localization of splicing factors in the cell cycle. Nature 369: 678-682.
Haizel, T., Merkel, T., Pay, A., Fejes, E., and Nagy, F. (1997) Characterization of proteins that interact with the GTP-bound form of the regulatory GTPase Ran in Arabidopsis. Plant J. 11: 93-103.
Hall, N. M., Craik, C.,and Hiraoka, Y. (1990) Homeodomain of yeast repressor alpha 2 contains a nuclear localization signal. Proc. Natl. Acad. Sci. USA 87: 6954-6958.
Hicks, G. R. and Raikhel, N. V. (1995) Nuclear localizaton signal binding protein in higher plant nuclei. Proc. Natl. Acad. Sci. USA 92: 734-738.
Hicks, G. R., Smith, H. M. S., Lobreaux, S., and Raikhel, N. V. (1996) Nuclear import in permeabilized protoplasts from higher plants has unique features. Plant Cell 8: 1337-1352.
Hirokawa, N. (1998) Kinesin and dynein superfamily proteins and the machenism of organall transport. Science 279: 519-526.
Hofmann, K., Bucher, P., Falquet, L., and Bairoch, A. (1999) The PROSITE database, its status in 1999. Nucl. Acids Res. 27: 215-9.
Hovland, R., Hesketh, J. E., and Pryme, I. F. (1996) The compartmentalization of protein synthesis: importance of cytoskeleton and role in mRNA targeting. Int. J. Biochem. Cell Biol. 28: 1089-1105.
Howard, E. A., Zupan, J. R., Citovsky, V., and Zambryski, P. C. (1992) The VirD2 protein of A. tumefaciens contains a C-terminal bipartite nuclear localization signal: implications for nuclear uptake of DNA in plant cells. Cell 68: 109-118.
Huang, J. C., Lin, S. M., and Wang, C. S. (2000) A pollen-specific and desiccation-associated transcript in Lilium longiflorum during development and stress. Plant Cell Physiol. 41: 477-485.
Hübner, S., Simith, H. M. S., Hu, W., Chan, C. K., Rihs, H.-P., Paschal, B., Raikhel, N. V., and Jeans, D. A. (1999) Plant importinα binds nuclear localization sequence with high affinity and mediates nuclear import independent of improtinβ. J. Biol.Chem. 247: 22610-22617.
Hurkman, W. J. and Tanaka, C. K. (1986) Solubilization of plant membrane proteins for analysis by two-dimensional gel electrophoresis. Plant Physiol. 81: 802-806.
Imagawa, M., Sakaue, R., Tanaba, A., Osada, S., and Nishihara, T. (2000) Two nuclear localization signals are required fir nuclear translocation of nuclear factor 1-A. FEBS Lett. 484: 118-124.
Iusem, N. D., Bartholomew, D. M., Hitz, W. D., and Scolnik, P. A. (1993) Tomato (Lycopersicon esculentum) transcript induced by water deficit and ripening. Plant Physiol. 102: 1353-1354.
Jans, D. A., Chan, C. K., and Hübner, S. (1998) Signals mediating nuclear targeting and their regulation: Application in drug delivery. Med. Res. Rev. 18: 189-223.
Jans, D. A. and Hübner, S. (1996) Regulation of protein transport to the nucleus: central role in phosphorylation. Physiol. Rev. 76: 651-685.
Jiang, C. J., Imamoto, N., Matsuki, R., Yoneda, Y., and Yamamoto, N. (1998) Functional characterization of a plant importinα homologue. J. Biol. Chem. 273: 24083-24087.
Johri, B. M. (1984) Embryology of angiosperms. Springer-Verlag, Berlin Heidelberg New York Tokyo, pp 53-76.
Kalderon, D., Roberts, B. L., Richardson, W. D., and Smith, A. E. (1984) A short amino acid sequence able to specify nuclear location. Cell 39: 499-509.
Kamalay, J. C. and Goldberg, R. B. (1980) Regulation of structural gene expression in tobacco. Cell. 19: 935-946.
Kataoka, N., Bachorik, J. L., and Dreyfuss, D. (1999) Transportin-SR, a nuclear import receptor for SR protein. J. Cell Biol. 145: 1145-1152.
Kinkema, M., Fan, W., and Dong, X. (2000) Nuclear localization of NPR1 is required for activation of PR gene expression. Plant Cell 12: 2339-2350.
Kiyosue, T., Yamaguchi-Shinozaki, K., and Shinozaki, K. (1994) Cloning of cDNA for genes that are early-responsive to dehydration stress (ERDs) in Arabidopsis thaliana L. : identification of three ERDs as HSP cognate genes. Plant Mol. Biol. 25: 791-798.
Koizumi, M., Yamaguchi-Shinozaki, K., Tsuji, H., and Shinozaki, K. (1993) Structure and expression of two genes that encode distinct drought-inducible cysteine proteinases in Arabidopsis thaliana. Gene 129: 175-182.
Kutay, U., Bischoff, F. R., Kostka, S., Kraft, R., and Görlich, D. (1997) Export of importinα from the nucleus is mediated by a specific nuclear transport factor. Cell 90: 1061-1071.
Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
Leffel, S. M., Marbon, S. A., and Neal, C. S. Jr (1997) Applications of green fluorescent protein in plants. Bio. Techinques 23: 912-918.
Makkerh, J. S., Dingwall, C., and Laskey, R. A. (1996) Comparative mutagenesis of nuclear localization signals reveals the importance of neutral and acidic amino acids. Curr. Biol. 6: 1025-1027.
Mascarenhas, J. P. (1990) Gene activity during pollen development. Annu. Rev. Plant Physiol. 41: 317-338.
Masuta, C., Yamana, T., Tacahashi, Y., Uyeda, I., Sato, M., Ueda, S., and Matsumura, T. (2000) Development of clover yellow vein virus as an efficient, stable gene-expression system for legume species. Plant J. 23: 539-546.
McCormick, S. (1993) Male Gametophyte Development. Plant Cell 5: 1265-1275.
Melchior, F. and Gerace, L., (1998) Two-way trafficking with Ran. Trends Cell Biol. 8:175-179.
Merkle, T., Haizel, T., Matsumoto, T., Harter, K., Dallmann, G., and Nagy, F. (1994) Phenotype of the fission yeast cell cycle regulatory mutant pim1-46 is suppressed by a tobacco cDNA encoding a small, Ran-like GTP-binding protein. Plant J. 6: 555-565.
Mermall, V., Post, P. L., and Mooseker, M, S. (1998) Unconventional myosins in cell movement, membrane traffic, and signal transduction. Science 279: 527-533.
Mundy, J. and Chua, N. H. (1988) Abscisic acid and water-stress induce the expression of a novel rice gene. EMBO J. 7: 2279-86.
Nagatani, A. (1998) Regulated nuclear targeting. Curr. Opin. Plant Biol. 1: 470-474.
Nigg, E. A. (1997) Nucleocytoplasmic transport: signals, mechanism and regulation. Nature 386: 779-787.
Niogret, M. F., Dubald, M., Mandaron, P., and Mache, R. (1991) Characterization of pollen polygalacturonase encoded by several cDNA clones in maize. Plant Mol. Biol. 17: 1155-64.
Nordin, K., Vahala, T., and Palva, E. T. (1993) Differential expression of two related, low-temperature-induced genes in Arabidopsis thaliana (L.) Heynh. Plant Mol. Biol. 21: 641-653.
Parra, M. M., del Pozo, O., Luna, R., Godoy, J. A., and Pintor-Toro, J. A. (1996) Structure of the dehydrin tas14 gene of tomato and its developmental and environmental regulation in transgenic tobacco. Plant Mol. Biol. 32: 453-60.
Rexach, M. and Blobel, G. (1995) Protein import into nuclei: association and dissociation reactions involving transport substrate, transport factors, and nucleoporins. Cell 83: 683-692.
Ribbeck, K., Lipowsky, G., Kent, H. M., Stewart, M., and Görlich, D. (1998) NTF2 mediates nuclear import of Ran. EMBO J. 17: 6587-6598.
Robbins, J., Dilworth, S. M., Laskey, R. A., and Dingwall, C. (1991) Two interdependent basic domains in nucleoplasmin nuclear targeting sequence: identification of a class of bipartite nuclear targeting sequence. Cell 64: 615-623.
Rouse, D. T., Marotta, R., and Parish, R. W. (1996) Promoter and expression studies on an Arabidopsis thaliana dehydrin gene. FEBS Lett. 381: 252-256.
Saalbach, G. and Christov, V. (1994) Sequence of a plant cDNA from Vicia faba encoding a novel Ran-related GTP-binding protein. Plant Mol. Biol. 24: 969-972.
Sambrook, J. E., Fritsch, E. T., and Maniatis, R. (1989) Molecular cloning: A laboratory manual. Cold Spring Harbor Laboratory. New York.
Sanford, J. C., Smith, F. D., and Russell, J. A. (1993) Optimizing the biolistic process for different biological applications. Methods Enzym. 27: 483-509.
Sasaya, T., Torrance, L., Cowan, G., and Ziegler, A. (2000) Aphid transmission studies using helper component proteins of potato virus Y expressed from a vector derived from potato virus X. J. Gen. Virol. 81: 1115-1119.
Schledz, M., Leclerc, D., Neuhaus, G., and Merkel, T. (1998) Characterization of four cDNAs encoding different importin alpha homologs from Arabidopsis. Plant Phsiol. 116: 868.
Scott, R. (1994) Anther development: a molecular perspective. In the molecular biology of flowering. Brian R. J. Eds. pp 141-193. CAB International. UK.
Silhavy, D., Hutvágner, G., Barta, E., and Bánfalvi, Z. (1995) Isolation and characterization of a water-stress-inducible cDNA clone from Solanum chacoense. Plant Mol. Biol. 27: 587-595.
Shoji, K., Iwasaki, T., Matsuki, R., Miyao, M., and Yamamoto, N. (1998) Cloning of a cDNA encoding an importin-alpha and down-regulation of the gene by light in rice leaves. Gene 212: 279-286.
Singh, M. O., O’Neill, P., and Knox, R. B. (1985) Inhibition of postmeiotic b-galactosidase synthesis during microsporogenesis in oilseed rape. Plant Physiol. 77: 225-228.
Sodeik, B., Ebersold, M. W., and Helenius, A. (1997) Microtubule-mediated transport of incoming herpes simplex virus 1 capsids to the nucleus. J. Cell. Biol. 136: 1007-1021.
Stacy, R. A. and Aalen, R. B. (1998) Identification of sequence homology between the internal hydrophilic repeated motifs of group 1 late-embryogenesis-abundant proteins in plants and hydrophilic repeats of the general stress protein GsiB of Bacillus subtilis. Planta. 206: 476-478.
Stacy, R. A., Espelund, M., Saeboe-Larssen, S., Hollung, K., Helliesen, E., and Jakobsen, K.S. (1995) Evolution of the Group 1 late embryogenesis abundant (Lea) genes: analysis of the Lea B19 gene family in barley. Plant Mol. Biol. 28: 1039-1054.
Stinson, J. and Mascarthas, J. (1985) Onset of alcohol dehydrogenase synthesis during microsporogenesis in maize. Plant Physiol. 77: 222-224.
Stoffler, D., Fahrenkrog, B., and Aebi, U. (1999) The nuclear pore complex: from molecular architecture to functional dynamics. Curr. Opin. Cell Biol. 11: 391-401.
Straub, P. F., Shen, Q., and Ho, T. D. (1994) Structure and promoter analysis of an ABA- and stress-regulated barley gene, HVA1. Plant Mol. Biol. 26: 617-630.
Takada, T., Iida, K., Awaji, T., Itoh, K., Takahashi, R., Shivui, A., Yoshida, K., Sugano, S., and Tsujimoto, G. (1997) Selective production of transgenic mice using green fluorescent protein as a marker. Natl. Biotechnol. 15: 458-461.
Twell, D., Soon, K. P., and Eric, L. (1998) Asymmertric division and cell-fate determination in developing pollen. Trends Plant Sci. 3: 305-310.
Varagona, M. J., Schmidt, R. J., and Raikhel, N. V. (1992) Nuclear localization signal(s) required for nuclear targeting of the maize regulatory protein Opaque-2. Plant Cell 4: 1213-1227.
Verwoerd, T.C., Dekker, B.M.M., and Hoekema, A. (1989) A small-scale procedure for the rapid isolation of plant RNAs. Nucl. Acids Res. 17: 2362.
Wang, C. S., Liau, Y. E., Huang, J. C., Wu, T. D., Su, C. C., and Lin, C.H. (1998) Characterization of a desiccation-related protein in lily pollen during development and stress. Plant Cell Physiol. 39: 1307-1314.
Wang, C. S., Lin, S. M., Wei, S. L. (1999) A stress-induceble protein associated with desiccation in lily pollen. Bot. Bull. Acad. Sin. 40: 199-205
Wang, C. S., Walling, L. L., Eckard, K. J., and Lord, E. M. (1992) Immunological characterization of a tapetal protein in developing anthers of Lilium longiflorum. Plant Physiol. 99: 822-829.
Wang, C. S., Wu, T. D., Chung, C. K. W., and Lord, E. M. (1996) Two classes of pollen-specific, heat-stable proteins in Lilium longiflorum. Physiol. Plant. 97: 643-650.
Wilkins, T. A., Bednarek, S. Y., and Raikhel, N. V. (1990) Role of propeptide glycan in post-translational processing and transport of barley lectin to vacuoles in transgenic tobacco. Plant Cell 2: 301-313.
Willing, R. P., Bashe, D., and Mascarenhas, J. P. (1984) An analysis of the complexity and diversity of mRNAs from pollen and shoots of Tradescantia. Plant Physiol. 75: 865-868.
Xu, H., Swoboda I., Bhalla, P. L., and Singh, M. B. (1999) Male gametic cell-specific expression of H2A and H3 histone genes. Plant Mol. Biol. 39: 607-614.
Yamaguchi-Shinozaki, K. and Shinozaki, K. (1993) The plant hormone abscisic acid mediates the drought-induced expression but not seed-specific expression of rd33, a gene responsive to dehyration stress in Arabidopsis thaliana. Mol. Gen. Genet. 238:17-25.
Zhao, Y., Hammond, J., Tousignant, M. E., and Hammond, R. W. (2000) Development and evaluation of a complementation-dependent gene delivery system based on cucumber mosaic virus. Arch. Virol. 145: 2285-2295

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