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研究生:陳勃聿
研究生(外文):Po -Yu Chen
論文名稱:TDZ誘導落花生種間雜種未熟胚形成多芽體及植株再生之研究
論文名稱(外文):Induction of multiple shoots from the immature embryos of interspeciefic hybrids by Thidiazuron and plants regeneration in peanut.
指導教授:胡 澤 寬
指導教授(外文):Tzer-Kaun Hu
學位類別:碩士
校院名稱:國立中興大學
系所名稱:農藝學系
學門:農業科學學門
學類:一般農業學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:中文
論文頁數:58
中文關鍵詞:TDZ落花生種間雜交
外文關鍵詞:TDZpeanutinterspeciefic cross
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  • 被引用被引用:4
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本試驗將落花生栽培種台南11號(A. hypogaea cv. TN11)和六個野生種落花生A. cardenasii、A. rigonii、A. chacoense、A. corrotina、A. spegazzinii及A. monticola之種間雜種未熟胚先經添加4 mg/l TDZ之MS培養基中誘導多芽體,再取其芽體及芽原基為培殖體,探討不同TDZ濃度誘導多芽體的效果與其增殖效率,及種間雜種F1植株的再生方法。結果摘述如下:
1. 六個雜交組合的芽體培養於添加0、0.5、1、2及4 mg/l TDZ培養基誘導多芽體,芽體的形成率在TDZ濃度間及雜交組合間均有顯著性差異。而平均芽體數在濃度間及雜交組合間則無顯著差異,但添加TDZ培養基的平均芽原基數顯著高於未添加TDZ的培養基。芽原基可再經添加TDZ的培養基誘導多芽體,但其效率低於以芽體為培殖體者。
2. 所有雜交組合之芽體以含2mg/l TDZ培養基連續繼代培養3次,芽體形成率皆維持在50﹪以上,平均芽體數也極穩定。
3. 含TDZ培養基所誘導之芽體為葉片短小肥厚的多芽體,經單純MS培養基繼代後,可提高芽體形成率及獲得較正常的芽體。將芽體繼代培養於單純MS培養基,所誘導芽體之發根率高於含TDZ培養基所誘導者,根系也較旺盛粗大。
4. 六個雜交組合之芽體移至含50 mg/l 抗壞血酸的MS培養基中,發根率隨NAA添加濃度的提高而呈上升的趨勢,在0.5 mg/1 NAA時,有最高的發根率,且所誘導的根也更為旺盛。六個雜交組合的芽體發根率均隨培養次數的增加而有下降的趨勢。
5. 六個雜交組合發根小苗之健化回復率介於32.16~54.66﹪之間,這些再生小苗經染色體數及外表特徵鑑定證實均為種間雜種F1植株。
Summary
The immature embryos of interspecific hybrids between cultivar TN11 and six wild type(A. cardenasii, A. rigonii, A. chacoense, A. corrotina, A. spegazzinii and A. monticola) were cultured on MS+4 mg/l TDZ to induce multiple shoot and bud primordia. The collected bud and bud primordia was them used to investigate the effects of TDZ on shoots induction and proliferation, and to establish plant regeneration technique. The results were summarized as follows:
1. Buds of six interspecific hybrids cultured on MS medium containing different TDZ concentrations to induce multiple shoot. The induced percentage of shoot were significantly different among TDZ levels and crosses, but the averaged shoots were non-significant different. The mean numbers of bud primordia cultured on MS media supplemented with TDZ were significantly higher than on MS medium. Bud primordia cultured on MS media containing TDZ could induce shoots, but the efficiency was lower than buds as explants.
2. In all crosses, the induced percentage of shoot induced from shoot through three times subculture on MS+2 mg/l TDZ medium could maintain above 50%, and the averaged shoot was stabilized.
3. TDZ induced the small and thickened multiple shoots, but MS medium without TDZ could increase the induced percentage of shoot and obtained normal shoots. Shoots subcultured with the MS medium without TDZ have the higher rooting percentage compare to MS medium containing TDZ, and root system was more healthy.
4. If the shoots of six interspecific hybrids transferred to MS media containing 50 mg/l ascorbic acid and three levels of NAA, the rooting percentage increased with NAA concentration. The MS medium containing 0.5 mg/l NAA produced the highest percentage of rooting and have more healthy root system. The percentage of rooting of all interspcific crosses were reduced with the subculture generation increased.
5. The recovery percentages of the rooted plantlets were ranged from 32.16% to 54.66% in six interspecific crosses. These plants were demonstrated as interspecific hybrids by chromosome number and morphological characteristics.
中文摘要..........1
前言..............3
前人研究..........6
材料方法..........11
結果..............15
討論..............46
參考文獻..........50
英文摘要..........57
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