(3.237.97.64) 您好!臺灣時間:2021/03/03 01:55
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果

詳目顯示:::

我願授權國圖
: 
twitterline
研究生:王瑞章
研究生(外文):Rei-Cheng Wang
論文名稱:黑豆及毛豆未熟子葉培養體胚形成之研究
論文名稱(外文):In Vitro Somatic Embryogenesis of Immature Cotyledon of Black Soybean and Vegetable Soybean
指導教授:葉茂生葉茂生引用關係
指導教授(外文):Mau-Shing Yeh, Ph. D.
學位類別:碩士
校院名稱:國立中興大學
系所名稱:農藝學系
學門:農業科學學門
學類:一般農業學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:英文
論文頁數:73
中文關鍵詞:黑豆毛豆未熟子葉體胚形成胚性組織
外文關鍵詞:Black SoybeanVegetable SoybeanImmature CotyledonSomatic EmbryogenesisEmbryogenic tissue
相關次數:
  • 被引用被引用:0
  • 點閱點閱:353
  • 評分評分:系統版面圖檔系統版面圖檔系統版面圖檔系統版面圖檔系統版面圖檔
  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:0
中文摘要
本研究利用栽培種黑豆台南三號(TN3)及毛豆高雄選一號(KS1)之未成熟種子(長度4-5mm)之子葉為材料,接種於以MSB為基礎培養基,各添加40mg/l 2,4-D及8g/l agar(代號M0a),2g/l gelrite(M0g),以及再各添加10g/l (M1a,M1g),30g/l(M2a,M2g),60g/l sucrose(M3a,M3g)等8種培養基;進行黑豆及毛豆未熟子葉胚性組織與體胚之誘導,並以石臘切片觀察體胚之發育及分化之過程。並將胚性組織團塊繼代於FN Lite培養基,進行胚性懸浮培養試驗。期能建立栽培種黑豆及毛豆之體胚培養技術,以作為基因轉殖研究及遺傳育種時之參考,結果如下︰
1. 黑豆台南三號及毛豆高雄選一號之胚性組織形成率有極顯著差異,黑豆台南三號胚性組織形成率顯著高於毛豆高雄選一號。在不同培養基間,胚性組織形成率亦達極顯著差異,含6%蔗糖及2g/l gelrite 之M3g培養基與含3%蔗糖及2g/l gelrite 之M2g培養基顯著高於其他培養基,未添加蔗糖之M0a及M0g對胚性組織誘導則皆無反應。
2. 胚性組織及球形胚之誘導在培養基與光照處理及品種之交感效應上均呈極顯著,而培養基之組成分的蔗糖及凝膠除在球形胚形成之誘導上與光照處理之交感效應不顯著外,與品種之交感作用及在胚性組織之誘導上均呈極顯著。黑豆台南三號之胚性組織形成率以在含6%蔗糖及2g/l gelrite 之M3g培養基,於暗處理2週後25μmol.m-2s-1 16小時照光6週環境下,其所誘導之胚性組織之形成率71.67%及球形胚46.67%(2.36個)最高,其次為含3%蔗糖及2g/l gelrite 之M2g培養基所誘導胚性組織之形成率33.33%及球形胚23.33%(2.5個)。毛豆高雄選一號則以含3%蔗糖及2g/l gelrite之 M2g培養基,於5-10μmol.m-2s-1 24小時照光8週環境下,誘導胚性組織形成率50%及球形胚36.67%(1.68個)最高。
3. 培養8週後,黑豆台南三號於不同培養基下,其所誘導體胚形態多為胚性組織及球形期胚。毛豆高雄選一號則由含6%蔗糖及8g/l agar 之M3a培養基、含3%蔗糖及2g/l gelrite 之M2g培養基及含6%蔗糖及2g/l gelrite 之M3g培養基誘導結果可看到胚性組織、球形期胚、魚雷期及子葉期胚。
4. 懸浮培養對黑豆及毛豆體胚增殖效果:球形胚增殖倍率黑
豆台南三號為2.8倍,優於毛豆高雄選一號之1.8倍。每個胚性組織平均形成球形胚數黑豆台南三號為3.2個,毛豆KS1則為2.6個。
5.組織學上的觀察,黑豆台南三號及毛豆高雄選一號未熟子葉培養八週後,皆可產生胚性組織及球形期、魚雷期之體胚。黑豆台南三號於培養第七週後開始產生胚性組織,八週後產生球、魚雷期之體胚。

In Vitro Somatic Embryogenesis of Immature Cotyledon of Black Soybean and Vegetable Soybean
Summary
The purposes of the experiments that are to establish a tissue culture system for somatic embryogenesis from immature cotyledon of soybean, and the results will be applied on inducing transgenic plant from explant and embryonic rescue of plant breeding. The explants, immature cotyledons of cv. TN3 of black soybean and cv.KS1 of vegetable soybean, were cultured on modified MSB basal medium containing 2,4-D and other nutrients to induce somatic embryo.
The results are summarized as follows:
1. The formative rates of embryonic tissue of cv. TN3 was significantly higher than it of cv.KS1 . The formative rates of embryonic tissue from immature cotyledons cultured on both M3g and M2g media were significantly higher than others.
2. There were significant interaction between medium and variety on formative rates both embryonic tissue and globular embryo. As to interaction between medium and light was also significantly influential on formation both embryonic tissue and globular embryo.
3. Most of immature cotyledons both cv. TN3 and cv.KS1 cultured in media formated embryonic tissue and globular embryo at 8 weeks olds of explant. However, embryonic tissue, globular embryos and monocotyledonous embryos were only induced with M2g,M3g and M3a media.
4. The formations ratioes of somatic embryos with suspension culture of cv. KS1 and cv. TN3 were 2.8 and 1.8, respectively, and the formations ratios of globular embryos were 2.6 and 3.2, respectively.
5. The differentiation of embryonic tissue from cotyledons of cv. TN3 appeaned at 7 weeks old of explants, and globular embryo and torpedo stage occurred at 8 weeks old of explants.

目錄
頁次
前 言………………………………………… 1
前人研究………………………………………… 3
材料與方法………………………………………20
結 果…………………………………………28
討 論…………………………………………52
中文摘要…………………………………………58
英文摘要…………………………………………60
參考文獻…………………………………………62

參考文獻
全中和、葉茂生。1991。大豆未熟胚培養之研究IV、不同培養基對大豆未熟胚軸及子葉之體胚與器官形成的影響中華農藝:237~249。
全中和、葉茂生。1994。大豆未熟胚培養之研究VII、未熟胚培養之器官形成及體胚形成上組織學的觀察。中華農學會報新166:6-15。
黃學林、李筱菊。2000。植物體細胞胚胎發生。科學農業48:120~127。
張平順、葉茂生。1991。大豆Soja亞屬不同基因型之多芽體形成能力的變異。農林學報40(2):75~86。
張平順、葉茂生。1992。大豆未熟胚培養之研究VI、大豆Soja亞屬不同基因型體胚形成能力的研究。農林學報41(1):23~36。
葉茂生。1989。大豆未熟胚培養之研究I、不同胚齡之未熟胚的體細胞胚形成及再生能力的比較。農林學報38(2):139~150。
葉茂生、全中和。1991。大豆未熟胚培養之研究III、未熟胚軸培養器官形成之探討。農林學報40(1):77~90。
葉茂生、全中和。1992。大豆未熟胚培養之研究V、瓊脂或凝膠及酪素水解物對大豆未熟胚軸及子葉之體胚與器官形成的影響。農林學報41(1):13~22。
蔡淑華。1988。植物組織切片技術綱要。72pp.。茂昌圖書有限公司,台北。
Amberger, L. A., R. G. Palmer, and R. C. Shoemaker. 1992. Analysis of culture-induced variation in soybean. Crop Sci. 32:1103-1108.
Bailey, M. A., H. R. Boerma, and W. A. Parrott. 1993a. Genotype effects on proliferative embryogenesis and plant regeneration of soybean. In Vitro Cell. Dev. Biol. 29P:102-108.
Bailey, M. A., H. R. Boerma, and W. A. Parrott. 1993b. Genotype-specific optimization of plant regeneration from somatic embryos of soybean. Plant Sci. 93:117-120.
Barwale, U. B., H. R. Kerns, and J. M. Widholm. 1986a. Plant regeneration from callus cultures of several soybean genotypes via embryogenesis and organogenesis. Planta 167:473-481.
Barwale, U. B., M. M. Meyer, Jr. and J. M. Widholm. 1986b. Screening of Glycine max and Glycine soja genotypes for multiple shoot formation at the cotyledonary node. Theor. Appl. Genet. 72:423-428.
Barwale, U. B. and J. M. Widholm. 1987. Somaclonal variation in plants regenerated from cultures of soybean. Plant Cell Rep. 6:365-368.
Bayley, J. K., J. King, and O. L. Gamborg. 1972. The ability of amino compounds and conditional medium to suspension cultures. Planta (Berl.) 105:25-32.
Beversdorf, W. D., S. L. Kimbal, and E. T. Bingham. 1975. Soybean tissue culture studies. Soybean Genet. Newsl. 2:51-52.
Blaydes, D. F. 1966. Interaction of kinetin and various inhibitors in the growth of soybean tissue. Physiol. Plant. 19:748-753.
Buchheim, J. A., S. M. Colburn, and J. P. Ranch. 1989. Maturation of soybean somatic embryos and the transition to plantlet growth. Plant Physiol. 89:768-775.
Buenrostro-Nava, M. T., H. M. Frantz, P. P. Ling, and J. J. Finer. 1999. Animation and image analysis for evaluation of soybean (Glycine max L. Merrill.) somatic embryo growth. Soybean Genet. Newsl. 26:http://www.soygenetics.org/articles/sgn1999-015.htm
Christianson, M.L., D.A. Warnick, and P.S. Carlson. 1983. A morphogenetically competent soybean suspension culture. Science 222:632-634.
Christou, P. and N. S. Yang. 1989. Developmental aspects of soybean (Glycine max) somatic embryogenesis. Ann. Bot. 64:225-234.
Dahmer, M. L., D. F. Hildebrand, and G. B. Collins. 1992. Comparative protein accumulation patterns in soybean somatic and zygotic embryos. In Vitro Cell. Dev. Biol. 28P:106-114.
Evans, D. A. 1981. Soybena tissue culture. Soybean Genet. Newsl. 8:27~29.
Finer, J. J. and M. D. McMullen. 1991. Transformation of soybean via particle bombardment of embryogenic suspension cultures. In Vitro Cell. Dev. Biol. 27P:175-182.
Finer, J. J. 1988. Apical proliferation of embryogenic tissue of soybean [Glycine max (L.) Merrill]. Plant Cell Rep. 7:238-241.
Finer, J. J. and A. Nagasawa. 1988. Development of an embryogenic suspension culture of soybean (Glycine max Merrill.). Plant Cell Tiss. Organ Cult. 15:125-136.
Gamborg, O. L., R. A. Miller, and K. Ojima.1968. Nutrient requirements of surpension cultures of soybean root cells. Exp. Cell Res. 50:151-158.
Gamborg, O. L., B. P. Davis, and R. W. Stahlquist. 1983. Somatic embryogenesis in cell cultures of Glycine species. Plant Cell Rep. 2:209-212.
Ghazi, T. D., H. V. Cheema, and M. W. Nabors. 1986. Somatic embryogenesis and plant regeneration from embryogenic callus of soybean, Glycine max L. Plant Cell Rep. 5:452-456.
Grant, J. 1984. Plant regeneration from cotyledary tissue of Glycine canesicens, a perennial wild relative of soybean. Plant Cell Tiss. Organ Cult. 3:169-173.
Graybosch, R. A., M. E. Edge, and X. Delannay. 1987. Somaclonal variation in soybean plants regenerated from the cotyledonary node tissue culture system. Crop Sci. 27:803-806.
Hammatt, N. and M. R. Davey. 1987. Somatic embryogenesis and plant regeneration from cultured zygotic embryos of soybean (Glycine max L. Merr.). J. Plant Physiol. 128:219-226.
Hammatt, N., M. R. Davey and R. S. Nelson. 1986. Plant regeneration from seedling cotyledons, leaves and petioles of Glycine candestine. Physiol. Plant. 68:125-128.
Hartweck, L. M., P. A. Lazzeri, D. Cui, G. B. Collins, and E.G. Williams. 1988. Auxin-orientation effects on somatic embryogenesis from immature soybean cotyledons. In Vitro Cell. Dev. Biol. 24:821-828.
Hepher, A., M. E. Boulter, N. Harris, and R. S. Nelson. 1988. Development of a superficial meristem during somatic enbryogenesis from immature cotyledons of soybean (Glycine max L.). Ann. Bot. 62:513-519.
Hildebrand, D. F., T. R. Adams, M. L. Dahmer, E. G. Williams, and G. B. Coillins. 1989. Analysis of lipid composition and morphological characteristics in soybean regenerants. Plant Cell Rep. 7:701-703.
Invers, D. R., R. G. Palmer, and W. R. Fehr. 1974. Anther culture in soybeans. Crop Sci. 891-893.
Kaltchuk-Santos, E., J. E. Mariath, E. Mundstock, C. Y. Hu, and M. H. Bodanese-Zanettini. 1997. Cytological analysis of early microspore divisions and embryo formation in cultured soybean anthers. Plant Cell Tiss. Organ Cult. 49:107-115.
Kerns, H. R., U. B. Barwale, M. M. J. Meyer, and J. M. Widholm. 1986. Correlation of cotyledonary node shoot proliferation and somatic embryoid development in suspension cultures of soybean [Glycine max (L.) Merr.]. Plant Cell Rep. 5:140-143.
Komatsuda, T. 1990. Ability of soybean (Glycine max L. Merr.) to produce somatic embryos on a medium containing a low concentration of sucrose. Japan J. Breed. 40:371-375.
Komatsuda, T. and K. Ohyama. 1988. Genotypes of high competence for somatic embryogenesis and plant regeneration in soybean Glycine max. Theor. Appl. Genet. 75:695-700.
Komatsuda, T., K. Kaneko, and S. Oka. 1991. Genotype x sucrose interactions for somatic embryogenesis in soybean. Crop Sci. 31:333-337.
Komatsuda, T. and S. W. Ko. 1990. Screening of soybean [Glycine max (L.) Merrill] genotypes for somatic embryo production from immature embryo. Japan J. Breed. 40:249-251.
Komatsuda, T., W. Lee, and S. Oka. 1992. Maturation and germination of somatic embryos as affected by sucrose and plant growth regulators in soybeans Glycine gracilis Skvortz and Glycine max (L.) Merr. Plant Cell Tiss. Organ Cult. 28:103-113.
Larkin, P. J., and W. R. Scowcroft. 1981. Somaclonal variation:A novel source of variability from cell cultures for plant improvement. Theor. Appl. Genet. 60:197-214.
Lazzeri, P. A., D. F. Hildebrand, and G. B. Collins. 1985. A procedure for plant regeneration from immature cotyledon tissue of soybean. Plant Mol. Biol. Rep. 3:160-167.
Lazzeri, P. A., D. F. Hildebrand, and G. B. Collins. 1987a. Soybean somatic embryogenesis: effects of nutritional, physical and chemical factors. Plant Mol. Biol. Rep. 10:209-220.
Lazzeri, P. A., D. F. Hildebrand, and G. B. Collins. 1987b. Soybean somatic embryogenesis: effects of hormones and culture manipulations. Plant Mol. Biol. Rep. 10:197-208.
Lazzeri, P. A., D. F. Hildebrand, J. Sunega, E. G. Williams, and G. B. Collins. 1988. Soybean somatic embryogenesis: interactions between sucrose and auxin. Plant Cell Rep. 7:517-520.
Li, B. J., W. H. R. Langridge, and A. A. Szalay. 1985. Somatic embryogenesis and plantlet regeneration in the soybean, Glycine max. Plant Cell Rep. 4:344-347.
Li, J., and E. A. Grabau. 1996. Comparison of somatic embryogenesis and embryo conversion in commercial soybean cultivars. Plant Cell Tiss. Organ Cult. 44:87-89.
Lippmann, B. and G. Lippmann. 1984. Induction of somatic embryos in cotyledonary tissue of soybean, Glycine max (L.) Merr. Plant Cell Rep. 3:215-218.
Liu, W., D. F. Hildebrand, P. J. Moore, and G. B. Collins. 1994. Expression of desiccation-induced and lipoxygenase genes during the transition from the maturation to the germination phases in soybean somatic embryos. Planta 194:69-76.
Liu, W., P. J. Moore, and G. B. Collins. 1992. Somatic embryogenesis in soybean via somatic embryo cycling. In Vitro Cell. Dev. Biol. 28P:153-160.
Miller, C. O. 1965. Evidence for the natural occurrence of zeatin and derivatives:Compounds from maize which promote cell division. Botany 54:473-479.
Murashige, T. and F. Skoog, 1962. A revised medium for rapid growth and bioassay with tabacco tissue culture. Physiol. Plant. 15: 473~479.
Nadolska-Orczyk, A. and W. Orczyk. 1994. New aspects of soybean somatic embryogenesis. Euphytica 80:137-143.
Oswald, T. H., A. E. Smith, and D. V. Philips. 1977. Callus and plantlet regeneration from cell culture of ladino clover and soybean. Physiol. Plant. 39:129-134.
Parrott, W. A., J. N. All, M. J. Adang, M. A. Bailey, H. R. Boerma, and C. N. Stewart, Jr. 1994. Recovery and evaluation of soybean [Glycine max (L.) Merr.] plants transgenic for a Bacillus thuringiensis var. kurstaki insecticidal gene. In Vitro Cell. Dev. Biol. 30P:144-149.
Parrott Lab. at UGA. 2000. Somatic embryogenesis of soybean.
http://mars.cropsoil.uga.edu/homesoybean/somprot.htm
11pp.
Parrott, W. A., L. M. Hoffman, D. F. Hildebrand, E. G. Williams, and G. B. Collins. 1989a. Recovery of primary transformants of soybean. Plant Cell Rep. 7:615-617.
Parrott, W. A., E. G. Williams, D. F. Hildebrand, and G. B. Collins. 1989b. Effect of genotype on somatic embryogenesis from immature cotyledons of soybean. Plant Cell Tiss. Organ Cult. 16:15-21.
Phillips, G. C. and G. B. Collins. 1981. Induction and development of somatic embryos from cell suspension cultures of soybean. Plant Cell Tiss. Organ Cult. 1:123-129.
Raghavan, V. 1985. The applications of embryo rescue in agriculture. Proceeding of the Intercenter Seminer on International Research Centers (IARCS) and Biotechnology. pp. 189-197. IRRI.
Rajasekaran, K. and J. W. Pellow. 1997. Somatic embryogenesis from cultured epicotyls and primary leaves of soybean [Glycine max (L.) Merrill]. In Vitro Cell Dev. Biol.- Plant 33:88-91.
Ranch, J. P., L. Oglesby, and A. C. Zielinski. 1985. Plant regeneration from embryo-derived tissue cultures of soybean by somatic embryogenesis. In Vitro Cell. Dev. Biol. 21:653-657.
Ranch, J. P., L. Oglesby, and A. C. Zielinski. 1986. Plant regeneration from tissue cultures of soybean by somatic embryogenesis. pp. 97-110. In: I. K. Vasil (ed.) Cell Culture and Somatic Cell Genetics of Plants. Academic Press, New York.
Samoylov, V. M., D. M. Tucker, and W. A. Parrott. 1998a. Soybean [Glycine max (L.) Merrill] embryogenic cultures: the role of sucrose and total nitrogen content on proliferation. In Vitro Cell Dev. Biol.- Plant 34:8-13.
Samoylov, V. M., D. M. Tucker,F. Thibaud-Nissen, and W. A. Parrott. 1998b. A liquid medium-based protocol for rapid regeneration from embryogenic soybean cultures. Plant Cell Rep. 18:49-54.
Santarem, E. R., and J. J. Finer. 1999. Transformation of soybean [Glycine max (L.) Merrill] using proliferative embryogenic tissue maintained on semi-solid medium. In Vitro Cell. Dev. Biol.-Plant 35:451-455.
Santarem, E. R., B. Pelissier, and J. J. Finer. 1997. Effect of explant orientation, pH, solidifying agent, and wounding on initiation of soybean somatic embryos. In Vitro Cell. Dev. Biol. Plant 33:13-19.
Santarer, E. R., and J. J. Finer. 1999. Transformation of soybean [Glycine max (L.) Merrill] using proliferative embryogenic tissue maintained on semi-solid medium. In Vitro Cell. Dev. Biol.-Plant 35:451-455.
Santos, K. G. B., E. Mundstock, and M. H. Bodanese-Zanettini. 1997. Genotype-specific normalization of soybean somatic embryogenesis through the use of an ethylene inhibitor. Plant Cell Rep. 16:859-864.
Sato, S., C. Newell, K. Kolacz, L. Tredo, J. Finer, and M. Hinchee. 1993. Stable transformation via particle bombardment in two different soybean regeneration systems. Plant Cell Rep. 12:408-413.
Sellars, R. M., G. M. Southward, and E. P. Gregory. 1990. Adventitious somtic embryogenesis from cultured immature zygotic embryos of peanut and soybean. Crop Sci. 30:408-414.
Shoemaker, R. C., L. A. Amberger, R. G. Palmer, L. Oglesby, and J. P. Ranch. 1991. Effect of 2,4-dichlorophenoxyacetic acid concentration on somatic embryogenesis and heritable variation in soybean [Glycine max (L) Merr.]. In Vitro Cell. Dev. Biol. 27P:84-88.
Shoemaker, R. C. and E. G. Hammond. 1988. Fatty acid composition of soybean [Glycine max (L.) Merr.] somatic embryos. In Vitro Cell. Dev. Biol. 24:829-832.
Slawinska, J. and R. L. Obendorf. 1991. Soybean somatic embryo maturation: composition, respiration and water relations. Seed Sci. Res. 1:251-262.
Stephens, P. A., U. Barwale-Sehr, C. D. Nickell, and J. M. Widholm. 1991. A ctyoplasmically inherited, wrinkled-leaf mutant in soybean. J. Hered. 82:71-73.
Stewart, C. N., Jr., M. J. Adang, J. N. All, H. R. Boerma, G. Cardineau, D. Tucker, and W. A. Parrott. 1996. Genetic transformation, recovery, and characterization of fertile soybean (Glycine max L.) Merrill) transgenic for a synthetic Bacillus thuringiensis CRYIA (c) gene. Plant Physiol. 112:121-129.
Thompson, J. F., Madison, J. T. and Muenster, A. M. E. 1977. In vitro culture of immature cotyledons of soybean (Glycine max L. Merr.). Ann Bot. 41:29-39.
Tian, L. N., D. C. W. Brown, H. Voldeng, and J. Webb. 1994. In vitro response and pedigree analysis for somatic embryogenesis of long-day photoperiod adapted soybean. Plant Cell Tiss. Organ Cult. 36:269-273.
Tilton, V. R. and S. H. Russell. 1984. In vitro culture of immature soybean embryos. J. Plant Physiol. 115:191-200.
Wang, C., K. P. C., and D. F. Hildebrand. 2001. α-Naphthaleneacetic induces the enpression of seedling lipoxygenases in cultured immature zygotic soybean embryo cotyledons. Plant Cell Rep. 20:85-91.
Williams, E. G. and G. Maheswaran. 1986. Somatic embryogenesis:Factors influencing coordinated behaviour of cells as an embryogenic group. Ann. Bot. 57:443-462.
Wright, M. S., K. L. Launis, R. Novitzky, J. H. Duesing, and C. T. Harms. 1991. A simple method for the recovery of multiple fertile plants from individual somatic embryos of soybean [Glycine max (L.) Merrill]. In Vitro Cell. Dev. Biol. 27P:153-157.
Wright, M. S., D. V. Ward, M. A. Hinchee, M. G. Carnes, and R. J. Kauf. 1987. Regeneration of soybean (Glycine max L. Merr.) from cultured primary leaf tissue. Plant Cell Rep. 6:83-89.

QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top
系統版面圖檔 系統版面圖檔