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研究生:黃鍵修
論文名稱:金絲桃屬植物抗氧化能力的比較研究
論文名稱(外文):Comparisons of antioxidant potentials of hypericum perforatum, hypericum sampsonii, and hypericum japonicum.
指導教授:陳宗禮陳宗禮引用關係
學位類別:碩士
校院名稱:國立中興大學
系所名稱:農藝學系
學門:農業科學學門
學類:一般農業學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:中文
論文頁數:57
中文關鍵詞:金絲桃屬抗氧化酵素抗氧化物
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本試驗分別以金絲桃屬植物中的貫葉連翹、元寶草及地耳草為材料,調查硫醇基、抗壞血酸、榖胱苷肽、總酚類化合物等抗氧化物質含量,超氧歧化酶(SOD)、穀胱苷肽還原酶(GR)、過氧化酶(POD)、抗壞血酸過氧化酶(APX)、脫氫抗壞血酸過氧化酶(DHAR)及榖胱苷肽過氧化酶(GPX)等抗氧化酵素活性及總抗氧化能力,同時比較熱風乾燥及冷凍乾燥處理後,材料所含的抗氧化物含量、抗氧化酵素活性及總抗氧化能力的改變,以提供開發金絲桃屬植物作為保健食品之參考。
結果顯示,三種金絲桃屬植物,硫醇基、抗壞血酸、榖胱苷肽、總酚類化合物等抗氧化物質含量皆有明顯的差異,其中元寶草>貫葉連翹>地耳草。Hypericin含量以及SOD、GR、POD、APX、DHAR及GPX等抗氧化酵素活性在於種之間有明顯差異,其中SOD活性明顯地高於其他酵素活性。比較不同乾燥的貫葉蓮翹,熱風乾燥之材料的抗氧化物質含量及hypericin含量增加,但抗氧化酵素活性及總抗氧化能力明顯降低。貫葉蓮翹hypericin及總抗氧化能力受萃取方式及溶劑影響,研磨萃取可得到較高的hypericin及總抗氧化能力,有機溶劑可萃取較多的hypericin。
Reactive oxygen free radical species (ROS) are highly reactive and potentially damaging transient chemical species formed in all cells. Cells are protected from ROS induced damage by a variety of endogenous ROS scavenging enzymes and chemical compounds. Recently there has been upsurge of interest in the therapeutic potential of medicinal plants as antioxidants in reducing such free radical induced tissue injury. In this regard, while the Hypericum perforatum, H. sampsonii, H. japonicum have long been used in herbal medicine for the treatment of burns, bruises, swelling, anxiety, antiaepressant, antinflammatory and antiseptic property, relatively little information is available concerning the antioxidant potential of these plant species. The objective of the present work was, therefore, to carry out a systematic survey of the relative levels of antioxidant potential in those selected medicinal plant species used in traditional or modern medical practice.
The results showed that all antioxidant properties including total thiol, glutathione, ascorbate, phenolic and hypericin content, the activities of superoxide dismutase, peroxidase, glutathione peroxidase, ascorbate peroxidase, dehydroascorbate reductase, and glutathione reductase were significantly difference among three selected medicinal plant species. The activity of superoxide dismutase was much higher than the others. Although the total antioxidant level and hypericin content in oven-dry material were higher than fresh tissue and lyophilized materials, but the antioxidant enzyme activities and total antioxidant capacity measured by the AAPH radical scavenging method were much lower than the others. Comparison the effects of extract solvent and extract methods indicated that acetone was a better solvent in the extraction of hypericin than water, and grinding extract was more efficiency to extract antioxidant substances which contributed the antioxidant capacity than infusion.
前言……………………………………………………1
前人研究……………………………………………6
材料與方法…………………………………………14
結果…………………………………………………24
討論…………………………………………………44
中文摘要……………………………………………49
英文摘要……………………………………………50
參考文獻……………………………………………52
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