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研究生:林惠雯
研究生(外文):Hui-Wen Lin
論文名稱:假性狂犬病毒UL41(vhs)基因的選殖表現及功能分析
論文名稱(外文):Cloning and functional expression of pseudorabies virus UL41 (vhs) gene
指導教授:張天傑
學位類別:碩士
校院名稱:國立中興大學
系所名稱:獸醫學系
學門:獸醫學門
學類:獸醫學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:中文
中文關鍵詞:假性狂犬病毒vhs蛋白UL41基因
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單純泡疹病毒一型(Herpes simplex virus type 1, HSV-1)之UL41基因產物virion host shutoff(vhs)蛋白,是病毒顆粒tegument的成分之一,感染細胞早期時會降解宿主mRNA,進而抑制宿主細胞蛋白質的合成。而假性狂犬病毒(pseudorabies virus, PRV)也有相同的基因。本實驗以假性狂犬病毒TNL株感染細胞後,純化病毒DNA,並以PCR的方法,得到1229 bp的產物;經DNA定序後,發現與Ka株vhs基因之核酸序列有97.8 %的相似性。再將所得產物分別選殖至原核表現載體pET32b與真核表現載體pcDNA3.1(-)。
為了確認我們所選殖的基因確實為vhs,我們將含vhs基因的pET32b表現載體(pET32b-vhs)送入大腸桿菌BL21(DE3)pLysS中進行表現。經IPTG誘導後,細菌的溶解液以SDS-PAGE分離蛋白質,再以鎳離子親和性色層分析法(affinity chromatography)將該表現產物純化,並以西方墨點法確認。結果顯示有一分子量為59 kDa的表現蛋白可被vhs蛋白之抗體所辨識。於是我們將 RNA marker和已純化之vhs蛋白一起反應,結果發現該蛋白確實有降解RNA 的能力。
由上述的結果得知,我們已成功選殖出PRV的vhs基因,接著我們利用chloramphenicol acetyltransferase (CAT) assay及plaque assay來檢測PRV的vhs基因在in vivo實驗中是否具有RNase的活性。我們將含vhs基因之載體pcDNA3.1-vhs及真核表現載體pCAT-Enhancer,一起轉染至細胞中,發現vhs確實會抑制CAT的表現量。另一方面,我們亦將vhs基因接在PRV的gE promoter(gEp)之後,利用轉染的技術將gEp-vhs送入已被PRV感染的細胞內,再以plaque assay來看vhs是否會抑制PRV病毒斑的形成。實驗結果顯示,vhs基因的表現能抑制PRV的病毒斑的形成達3.3倍之多。綜合上述的實驗結果可知,PRV的vhs是藉由降解細胞內的mRNA,進而抑制細胞內蛋白質的合成及病毒的複製。

目錄-------------------------------------------------------I
中文摘要---------------------------------------------------III
英文摘要---------------------------------------------------IV
圖次-------------------------------------------------------V
第一章、緒言-----------------------------------------------1
第二章、文獻探討-------------------------------------------2
第一節 假性狂犬病之歷史背景--------------------------------2
第二節 假性狂犬病毒之分類命名、型態、組成及其特性----------2
第三節 假性狂犬病毒之潛伏感染------------------------------3
第四節 假性狂犬病毒基因體的結構及目前已知基因的位置--------4
第五節 假性狂犬病毒基因的表現、基因產物及生物功能----------5
第六節 假性狂犬病毒醣蛋白的種類和特性----------------------8
第七節 假性狂犬病毒在感染細胞時之吸附(adsorption)、成熟(maturation)與釋出(egress)---------------------------------11
第八節 疱疹病毒的virion host shutoff (vhs) 基因的研究------12
第三章、材料與方法-----------------------------------------15
1. 病毒----------------------------------------------------15
2. 病毒增殖培養及純化--------------------------------------15
3. 載體之建構----------------------------------------------15
4. 原核表現及純化------------------------------------------17
5. 蛋白質膠體電泳------------------------------------------18
6. 西方墨點法----------------------------------------------19
7. N端胺基酸定序-------------------------------------------19
8. 試管內vhs 蛋白的RNase及DNase活性測試--------------------20
9. Chloramphenicol acetyl transferase (CAT) assay----------20
10. Plaque assay-------------------------------------------21
11. 抽取total RNA------------------------------------------21
12. 逆轉錄聚合酶連鎖反應(Reverse transcription polymerase chain reaction,RT-PCR)------------------------------------22
第四章、結果-----------------------------------------------23
第五章、討論-----------------------------------------------50
參考文獻---------------------------------------------------54

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