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研究生:黃俊展
論文名稱:環境中常在菌第1型integron之檢測
論文名稱(外文):The detection of class 1 integron in environmental microorganisms
指導教授:楊繼楊繼引用關係何素鵬
學位類別:碩士
校院名稱:國立中興大學
系所名稱:獸醫學系
學門:獸醫學門
學類:獸醫學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:中文
中文關鍵詞:抗藥性
外文關鍵詞:integron
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由於人類對於抗生素的廣泛利用,各種抗藥菌株的出現,使得我們不得不面對的如此重要的抗藥性問題。細菌發展出抗藥性甚至多重抗藥性,可能和細菌能獲得並傳遞外來的抗藥基因有關,而細菌獲得外來抗藥基因的模式,就一般所熟知,為藉可接合性抗藥性質體或帶有抗藥基因的跳躍子,將抗藥基因傳播給其他細菌。目前有學者提出一新的抗藥基因移動模式稱 “integron”。目前研究較多且在細菌中分布較廣的是第一型integron。為了探討目前在獸醫領域中,具致病性的細菌以及環境常在菌中抗藥性及第一型integron的情形,我們收集了25株Escherichia coli、25株Salmonella cholerasuis、32株Vibrio spp.及8株Edwardsiella tarda,以肉湯微稀釋法施行抗生素敏感性試驗,觀察抗藥性的情形。另利用聚合連鎖反應來偵測第一型integron,觀察其分布的情形。結果顯示在13株多重抗藥性的Escherichia coli中,檢測第一型integron有9株為陽性;在12株多重抗藥性的Salmonella cholerasuis中,有4株為陽性;在14株多重抗藥性的Vibrio spp.中,僅有1株為陽性;在1株多重抗藥性的Edwardsiella tarda中,檢測第一型integron的結果為陰性。
目錄
頁次
中文摘要‧‧‧ ‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧ I
英文摘要‧‧‧ ‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧ II
目錄‧‧‧‧‧ ‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧ III
表次‧‧‧‧‧ ‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧ IV
圖次‧‧‧‧‧ ‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧ V
壹、緒言‧‧‧ ‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧ 1
貳、文獻探討 ‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧ 3
參、材料與方法 ‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧ 12
肆、結果‧‧‧ ‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧ 21
伍、討論‧‧‧ ‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧ 26
陸、參考文獻‧ ‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧ 49
附錄一‧‧‧‧ ‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧‧ 56
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