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研究生:高源彬
研究生(外文):Yuan-Pin Kao
論文名稱:前花青素對streptozotocin誘發性糖尿病的影響
論文名稱(外文):Effect of proanthocyanidin on streptozotocin-induced diabetes
指導教授:陳昇明陳昇明引用關係高永旭
學位類別:碩士
校院名稱:國立中興大學
系所名稱:生命科學院碩士在職專班
學門:生命科學學門
學類:生物學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:中文
論文頁數:78
中文關鍵詞:前花青素胰島素依賴型糖尿病非胰島素依賴型糖尿病
外文關鍵詞:proanthocyanidinIDDMNIDDM
相關次數:
  • 被引用被引用:8
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摘 要
葡萄子前花青素是屬於生物類黃酮的一族,諸多研究顯示,具有抗氧化的作用,並且能減緩疾病的發生. 為了評估攝取葡萄是否有抗糖尿病的作用,我們以腹腔注射的方式,注射葡萄子萃取物(65%純度)至以STZ誘導產生糖尿病的雄性ICR老鼠體內中,處理十天後, 以糖尿病控制組比較,我們發現前花青素有意義的降低了糖尿病鼠血糖的濃度、體重、攝食量、排尿量及排糞量,但若以兒茶素與表兒茶素等結構上差異之單聚合體,處理糖尿病鼠則無此作用. 而依口服葡萄糖耐量試驗曲線圖來看,實驗顯示前花青素亦能增進葡萄糖之耐量. 我們亦發現糖尿病鼠,當前花青素停止處理時,血糖濃度會又會逐漸上昇,意味前花青素對降低血糖濃度的效果是可逆的. 另外何以前花青素會有抗糖尿病之作用,結果亦顯示,是因它能減緩STZ對胰島細胞的傷害. 總結的說對於糖尿病罹患率較低之某些歐洲國家或許與攝取葡萄之多寡有密切關係。
Abstract
Grape seed proanthocyanidins (OPC) are a family of polymerized bioflavanoids known to have influence on the antioxidant activities and the development of biomedical diseases. For clear assessment of the possible antidiabetic effects of grape consumption, we injected grape seed OPC extract (65% purity) intraperitoneally (ip) into diabetic male ICR mice which were induced ip by streptozotocin. We found that OPC, but not structurally-related catechin and epicatechin monomers, significantly reduced diabetic glucose levels, food intake, body weight, urine, and feces within 10 days as compared to the diabetic controls. Based on the oral glucose tolerance test, OPC also improved glucose hyperglycemia. The decrease in the diabetic blood glucose levels was reversible; when OPC administration was stopped, animals regained the diabetic glucose. The antidiabetic effect of OPC could be due to its increasing the number of pancreatic islets. It is possible that there is a relationship between the high consumption of grape and the low incidence of diabetes in some European countries.
目錄
頁次
中文摘要 1
英文摘要 2
縮寫字 3
壹、緒論 5
一、糖尿病 5
(一)定義 5
(二)流行病學 5
(三)分類 6
(四)可能的病因 6
二、前花青素 9
(一)歷史 9
(二)來源及分佈 10
(三)化學結構 11
(四)葡萄子內所含polyphenols之各組成 13
(五)生物合成 14
(六)吸收 15
(七)毒性 15
(八)代謝 15
(九)生物活性 16
三、研究動機與目的 26
貳、材料與方法
一 、實驗材料及設備 28

頁次
(一)動物品系及小白鼠飼料 28
(二)試藥與材料 28
(三)儀器設備 29
(四)OPC特性分析 30
(五)STZ致糖尿病的機制 32
(六)試劑製備 34
二、研究方法 36
(一)動物飼養及樣品收集 36
(二)誘導小白鼠糖尿病產生 36
(三)OPC依劑量對糖尿病的影響 37
(四)OPC對STZ誘發糖尿病reversible的研究 37
(五)OPC對糖尿病鼠葡萄糖耐量試驗 38
(六)預防糖尿病的研究 38
(七)前花青素單聚合體對血糖濃度的影響 39
(八)統計分析 40
參、結果 41
(一)以i.p STZ處理,誘導mice產生糖尿病, 血糖濃度的
變化 41
(二)OPC依劑量,對不同程度糖尿病鼠血糖、體重、攝食
量及飲水量之影響 41
(三)OPC對糖尿病鼠的血糖回復能力 43
(四)口服耐糖變化 43

頁次
(五)前花青素單聚合體對血糖濃度、體重、攝食量及飲
水量的變化 44
(六)糖尿病鼠經口服或經腹部注射OPC的比較 45
(七)OPC預防糖尿病之作用 45
(八)OPC對以STZ誘發糖尿病鼠Islet number的變化 46
(九)OPC依劑量,對糖尿病鼠生化因子與器官重量的變化 47
肆、討論 49
伍、結論 54
陸、表、圖目錄 55
表一.Flavonoid classes based on chemical characterizations 55
表二.前花青素單聚合體對糖尿病鼠體重、食物量及飲水
量的影響 56
表三.OPC以口服或i.p處理糖尿病鼠,血糖濃度、體重、
攝食量、飲水量、糞便量及尿液量的比較 57
表四.OPC處理依劑量對糖尿病生化因子及器官重量的
變化 58
圖一.類黃酮的基本結構 59
圖二.葡萄萃取物前花青素HPLC分析與化學結構 60
圖三,Streptozotocin(STZ)之化學結構 62
圖四.STZ誘發型糖尿病鼠血糖濃度的變化 63
圖五.OPC依劑量影響不同程度的STZ誘發型糖尿病鼠
之血糖濃度 64

頁次
圖六.OPC依劑量對不同濃度血糖之小白鼠體重的影響 65
圖七. OPC依劑量對不同血糖濃度之小白鼠攝食量的變化 66
圖八. OPC依劑量對不同血糖濃度之小白鼠飲水量的變化 67
圖九.OPC對糖尿病鼠,血糖濃度reversible的變化 68
圖十.OPC對糖尿病鼠,口服葡萄糖耐量試驗血糖濃度的
變化 69
圖十一.前花青素單聚合體對糖尿病鼠, 血糖濃度的
變化 70
圖十二.OPC對糖尿病的預防影響 71
圖十三.OPC依劑量對糖尿病鼠Islet number的影響 72
柒、參考文獻 73
一、英文參考文獻 73
二、中文參考文獻 78

柒、參考文獻
一、英文參考文獻
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