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研究生:林啟超
研究生(外文):Chi-Chau Lin
論文名稱:在攪拌式醱酵槽中加裝不織布對於Aspergillusflavus生產麴酸之影響
論文名稱(外文):The effect of equipping a non-woven fabrics in the fermenter on the production of kojic acid by Aspergillus flavus
指導教授:鄭智元鄭智元引用關係
指導教授(外文):Chu-Yuan Cheng
學位類別:碩士
校院名稱:國立成功大學
系所名稱:化學工程學系碩博士班
學門:工程學門
學類:化學工程學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:中文
論文頁數:70
中文關鍵詞:麴酸
外文關鍵詞:kojic acid
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本研究著重於評估不同麴菌的麴酸生產能力,從中選擇生產能力較佳者來探討醱酵條件對菌體生長型態與麴酸生產能力,並進一步討論在醱酵槽加裝不織布對麴酸生產的影嚮。所採用的不織布為”Polyfilling”噴交棉 (樹脂棉),乃利用PET回收料,經過再加工生產的聚酯再生棉。
在本研究所測試之5株黴菌菌株中,只有A. flavus CCRC 30010及A. oryzae CCRC 30102有較顯著量之麴酸產生,分別為24.4及6.86 g/l。而在YES broth中振盪培養時A. flavus CCRC 30010的菌體生長雖不如A. oryzae CCRC 30102,但其麴酸產量卻為A. oryzae CCRC 30102的5倍左右。當起始pH為6.5時A. flavus CCRC 30010麴酸之產生最佳而菌體之生長以pH為4.5時最佳。培養溫度則以25℃培養時,麴酸之產生與菌體之生長最佳。當以sucrose 為碳源時,A. flavus CCRC 30010麴酸之產量及菌體之生長最佳。氮源中以yeast extract 對麴酸之產生與菌體之生長最佳。在YES broth中,KH2PO4、 K2HPO4、 (NH)4HPO4、MgSO4˙7H2O之添加對A. flavus CCRC 30010麴酸及菌體之產生並無顯著性之影嚮。
在吾人之懸浮細胞批次醱酵槽實驗中,菌體於第3天才進入對數生長期末期,此時麴酸亦開始產生。而在接近第10天時麴酸的產量可達最高,其值為8.g/l。然而,在不織布系統中,麴酸生產的時間雖較晚(第4天)但在第11天時麴酸產量竟達20.85g/l。吾人亦得到一個結果,不織布的存在下可以增加菌絲體的完整性,有利麴酸的產生,使得菌體在醱酵槽中,以類似靜置培養的方式,能更快、更有效的生產麴酸。
This study intended to evaluate the productivity of different strains of fungi and to select a kojic acid producing strain with high productivity can be studied. Then we intend to produce kojic acid in the presence of nonwoven fabric and to investigate the effect. We used a nonwoven fabric (with the trade name of Polyfiling), which consists of PET.
Aspergillus flavus CCRC 30010 and A. oryzae CCRC 30102 produced significant highest amount of kojic acid (24.4&6.86 g/l) among the five tested strains of mold. More kojic acid production by A.flavus 30010 was noted in YES broth in shaking culture than A. oryzae CCRC 30102, although growth is less. YES broth with an initial pH of 6.5or a cultivation temperature of 25℃would result in the higher production of kojic acid by A. flavus CCRC 30010. While growth of A. flavus CCRC 30010 was best when cultivation was done with YES having an initial of pH of 4.5 and at 25℃. Greater growth and kojic acid production were observed when sucrose was used as the carbon source. Yeast extract was the best nitrogen source for the growth and kojic acid production by A. flavus CCRC 30010. In addition, it was found that addition of KH2PO4、 K2HPO4、 (NH)4HPO4、MgSO4˙7H2O to YES broth had no significant effects.
In the suspension fermentation, the cells entered the exponential growth end phase at 3 day, and the kojic acid production. The maximum kojic acid was 8g/l at 11 day. In contrast, the kojic acid was 20.85 at 11 day if using the non-woven-fabrics system. Based on these findings, the roles of the non-woven fabrics in the fermentation with A. flavus CCRC 30010 an improve completely of mold in the fermentation, and production kojic acid like static culture rapidly and effectively.
中文摘要Ⅰ
英文摘要Ⅲ
誌謝V
目錄Ⅵ
表目錄Ⅹ
圖目錄IX
第一章 緒論1
1-1 前言1
1-2 麴酸的介紹2
1-2-1 麴酸的結構2
1-2-2 麴酸的物理、化學性質2
1-2-3 麴酸的生物特性9
1-2-4 麴酸的安全性9
1-3麴酸的生合成途徑10
1-4 麴酸的應用15
1-5 不織布的應用17
1-6 研究目的19
第二章 實驗材料與方法20
2-1 實驗材料20
2-1-1 菌株20
2-1-2 培養基20
2-1-3 實驗儀器21
2-1-4 實驗藥品21
2-2 不織布的特性26
2-2-1 溫度對不織布的影嚮26
2-2-2 剪應力對不織布的影響26
2-2-3 不織布的疏水性27
2-3實驗步驟27
2-3-1菌株保存之雙重管的開封27
2-3-2 菌種保存、活化與接種源之製備28
2-3-3 麴酸生產菌株之篩選28
2-3-4 醱酵實驗29
(1) 振盪醱酵實驗29
a.自由細胞之振盪醱酵實驗29
b.置入不織布之振盪醱酵實驗29
(2) 醱酵槽實驗29
a.醱醱槽中自由細胞之批次醱酵實驗29
b.醱酵槽中置入不織布之批次醱酵實驗30
2-3-5 溶氧電極之校正30
2-4 分析方法31
2-4-1 菌體濃度之分析31
(1)自由細胞之菌體31
(2)不織布上之菌體31
2-4-2 培養液pH之測定31
2-4-3 麴酸之分析31
第三章 結果與討論35
3-1 麴酸生產菌株篩選.35
3-2 不同黴菌菌株在YES broth 振盪培養時之生長及麴酸之產生37
3-3 起始pH值對A. flavus CCRC 30010之生長及麴酸產生之影響42
3-4 培養溫度對A. flavus CCRC 30010之生長及麴酸產生之影響45
3-5 無機鹽類添加對A. flavus CCRC 30010之生長及麴酸產生之影響47
3-6 不同碳源對A. flavus CCRC 30010之生長及麴酸產生之影響48
3-7 不同氮源對A. flavus CCRC 30010之生長及麴酸產生之影響51
3-8 在搖瓶中置入不織布對麴酸產量之影響54
3-9 醱酵槽實驗55
3-9-1 懸浮細胞系統之批次醱酵57
3-9-2 在醱酵槽中置入不織布之批次醱酵57
第四章 結論64
參考文獻66
沈啟智,“在攪拌式醱酵槽中置入不織布對於Acinetobacer radioresistens 醱酵生產耐鹼性脂肪酵素的影響”,國立成功大學化學工程研究所碩士論文(1998)
張文亮, ”利用Aspergillus tamarii 生產麴酸” ,國立台灣大學食品科技研究所碩士論文(1995)
張文亮、周正俊, ”振盪與靜置培養下不同黴菌在二種培養基中之生長與麴酸之產量” ,食品科學, 23: 346-355(1996)
張文亮、周正俊, ”溫度、pH與一些化合物之添加對Aspergillus tamarii 麴酸產生之影響” ,食品科學, 23: 520-528(1996)
Ariff, AB;Salleh, MS; Ghani, B; Hassan, MA; Rusul, G and. Karim, MIA. Aeration and yeast extract requirements for kojic acid production by Aspergillus flavus link. Enzyme microb. Technol. 19: 545-550 (1996)
Baipai, P., Agrawala, P.K. and Lishwanathan, L. Enzymes relevant to kojic acid biosynthesis in Aspergillus flavus. J. Gen. Microbiol. 127: 131-136 (1981a)
Bajpai, P., Agrawala, P.K., and Vishwanathan, L. Optimization of fermentation parameters for kojic acid fermentation. Indian J. Microbiol. 21:42. (1981b)
Bajpai, P; Agrawala, PK; Vishwanathan, L. Production of kojic acid by resuspended myelia of Aspergillus flavus. Can. J. Microbiol. 28: 1340-1346 (1982a)
Bajpai, P., Agrawala, P.K., and Vishwanathan, L. Kojic acid : synthesis and properties. J. Sci. Ind. Res. 41: 185-194 (1982b)
Barham, H.N., and Smits, B.L. Production of kojic acid from xylose by Aspergillus flavus. Ind. Eng. Chem. 41:185. (1936)
Beard, R.L., and Walton, G.S. Kojic acid as an insecticidal mycotoxin. J. Invert. Pathol. 14:53. (1969)
Bentley, R. Preparation and analysis of kojic acid. Method Enzymol. 3: 238-241 (1957)
Bigelis, R. Flavor metabolites and enzymes from filamentous fungi. Food Technol. 46:151. (1992)
Birkimshaw, J.H., Charls, J.H.V., Lilly, C.H., and Raistricl, H. Studies in the biochemistry of micro-organisms. Part Ⅶ-Kojic acid (5-hydroxy-2- hydroxymethyl-γ-pyrone). Trans. Roy. Soc. London B220:127. (1931)
Chen, J.S., Wei, C., and Marshall, M.R. Inhibition mechanism of kojic acid on polyphenol oxidase. J. Agric. Food Chem. 39: 1897-1901. (1991a)
Coupland,K and Niehaus, WG. Effect of nitrongen supply ,Zn2+,and salt concentration on kojic acid and versicolorin biosynthesis by Aspergillus parasiticus. Exp. Mycol. 11: 206-213 (1987)
Detroy, R.W., Lillehoj, E.B., and Ciegler, A. Microbial Toxins, Ciegler, A., Kadis, S., and Aji, S.J. (Eds.) Academic Press, New York. p.68. (1971)
Foster , J.W. Chemical Activities of Fungi, Academic Press, New York p430-445 (1949)
Gould, B.S. The metabolism of Aspergillus tamarii Kita. Kojic acid production. Biochem. J. 32:797. (1938)
Iyengar, R; McEvily, AJ. Anti-browning agents: alternatives to the use of sulfites in foods. Trends Food Sci. Technol. 3: 60-64 (1992)
Katagiri, H., and Kitahara, K. The formation of kojic acid by Aspergillus oryzae. Bull. Agr. Chem. Soc. Japan 5:38. (1929)
Kawate, S., Koike, M., Fukuo, T., Hashiba, Y., and Ichikawa, T. Kojic acid fermentation. II. Nitrogen sources of kojic acid fermentation medium. Kogyo Kagaku Zasshi 72:513. (1969a)
Kawate, S., Fukuo, T., and Miyamoto, I. Kojic acid fermentation. Ⅳ. Effects of n-propyl alcohol as a stimulator for kojic acid-producing mold. Kogyo Kagaku Zasshi 74:2208. (1971)
Kwak, MY and Rhee, JS. Characteristics of immobilized Aspergillus oryzae for kojic acid production. Biotechnol. Bioeng. 39: 903-906 (1992)
Lee, H.F., and Rhee, J.S. controlled mycelial growth for kojic anid production using Ca-alginate-immobilized fungal cells. Appl. Microbiol. Biotechnol. 36:578. (1992b)
May, O.E., Moyer, A.J., Wells, P.A., and Herrick, H.T. The production of kojic acid by Aspergillus flavus. J. Am. Chem. Soc. 53:774. (1931)
Nandan, R., and Polasa, H. Inhibition of growth and kojic acid produced by Aspergillus luteovirescens. J. Bacteriol. 50:579. (1945)
Niwa, Y and Akamatsu, H. Kojic acid scavenges free radicals while Potentiating eukocyte functions including free radical generation. Inflammation 15: 303-316 (1991)
Ogawa, A; Wakisaka, Y; Tanaka, T and Nakanishi, K. Productin of kojic acid membrane-surface liquid culture of Aspergillus oryzae NRRL454. J. Ferment. Bioeng. 80: 41-45 (1995)
Ohyama, Y and Mishima, Y. Melanogenesis-inhibitory effect of kojic acid and its action mechanism. Fragrance J. 6: 53-58 (1990)
Sitaier, H., and I. Maliszewska. Porduction of exogenous lipase by bacteria, fungi and acitomycetes. Enzyme Microb. Technol., 10:492. (1990)
Takafumi, F., Mitsuyasu, O., Takayoshi, T., Kyouko, T., Toshio, M., and Yong, S.P. Improvement of production of kojic acid by a mutant strain Aspergillus oryzae, MK107-39 Journal of bioscience and bioengineering 91: 272-276 (2001)
Uchino, K; Nagawa, M; Tonosaki, Y; Oda, M and Fukuchi, A. Kojic acid as an anti-speck agent. Agric. Biol. Chem. 52: 2609-2610 (1988)
Wei, C.I., Huang, T.S., Chen, J.S., Marshall, M.R., and Chung, K.T. Production of kojic acid by Aspergillus candidus in three culture media. J. Food Prot. 54: 546-548 (1991a)
Wei, C.I., Huang, T.S., Fernando, S.Y., and Chung, K.T. Mutagenicity studies of kojic acid . Toxicol. Lett. 59:213. (1991b)
Werch, s.c., Oester, Y.T., and Friedemann,T.E. Kojic acid-acid convulsant. Science. 59:213. (1957)
Yahashi, Y., M. Hatsu, H. Horitsu, K. Kawai, T. Suzuki and K. Takamizawa. D-Glucose feeding for improvement of xylitol productivity from D-xylose using candida tropicalis immobilized on a non-woven fabric. Biotech. Lett.,18:1395-1400 (1996)
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