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研究生:王唯匡
研究生(外文):Wei-Kuang Wang
論文名稱:台灣特有水生植物大安水蓑衣的族群分化與親緣地理學之探討
論文名稱(外文):Population differentiation and phylogeogarphy of Hygrophila pogonocalyx (Acanthaceae) in Taiwan
指導教授:蔣鎮宇蔣鎮宇引用關係
指導教授(外文):Tzen-Yuh Chiang
學位類別:碩士
校院名稱:國立成功大學
系所名稱:生物學系碩博士班
學門:生命科學學門
學類:生物學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:中文
論文頁數:83
中文關鍵詞:逢機擴大多型性核酸大安水蓑衣親緣地理親緣網狀圖族群分化葉綠體去氧核糖核酸
外文關鍵詞:phylogeographyRAPDchloroplast DNAminimum spanning networkHygrophila pogonocalyxpopulation differentiation
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中文摘要
本研究藉由偵測葉綠體DNA之atpB及rbcL基因間的非轉譯區間序列和RAPD分子指紋變異來探討大安水蓑衣(Hygrophila pogonocalyx Hayata)的親緣地理型式,大安水蓑衣根據國際自然及自然資源保育聯盟(IUCN)之瀕危物種保育等級標準,已可列入嚴重瀕臨絕滅級(critically endangered)。由於大安水蓑衣主要依靠蜜蜂傳播花粉,其族群間基因交流可能受限於蟲媒物種的飛行能力,故推測西台灣與宜蘭的族群應具有地理上的隔離,本實驗共定序出8個族群70個個體葉綠體DNA之atpB及rbcL基因間的非轉譯區間的序列共計849鹼基對,而在RAPD指紋技術共計使用50個引子;從RAPD指紋技術資料進行統計分析,在階層性AMOVA分析中,顯示在地理區間(Φct = 0.934; P = 0.048),及族群間(Φst = 0.945; P < 0.001)皆有明顯的分化,而同一區域的不同族群間分化並不顯著(Φsc = 0.169; P < 0.001),利用葉綠體DNA資料估算族群間的基因交流值(Nm)和族群間分化指數(Fst),並將族群間地理距離與基因交流值做迴歸分析,結果呈現負相關,顯示大安水蓑衣其遺傳分化程度符合「isolation by distance」 模式(R= 0.745);根據由葉綠體DNA所構築的親緣網狀圖(minimum spanning network),檢測地理形式與clade間的關係,顯示大安水蓑衣的東西部兩大地理區所呈現的地理分佈模式,主要是受到過去棲地破碎化(past fragmentation)的影響,而距今五百萬年前中央山脈的開始隆起,可能是造成大安水蓑衣地理東西隔離的主因。
Abstract
Sequence variation of the atpB-rbcL noncoding spacer region of the chloroplast DNA and RAPD fingerprints were used to reconstruct the phylogeography of Hygrophila pogonocalyx. According to the criteria of the International Union for Conservation of Nature and Nature Resources, H. pogonocalyx is on the Red List Category due to its endangered status. Entomophilous plants of H. pogonocalyx are mostly pollinated by honeybees. Gene flow between populations is expected to be constrained by the migratory capacity of the pollinators. Hierarchical AMOVA analyses of RAPD fingerprints data, which used 50 random primers, indicated significant differentiation between geographical regions (Φct = 0.934; P = 0.048), among populations (Φst = 0.945; P < 0.001), and among populations within region (Φsc = 0.169; P < 0.001). A total of 849 bp of the cpDNA atpB-rbcL spacer was sequenced from 8 populations of H. pogonocalyx. Two geographically based groups, west and east area, were identified by a neighbor-joining tree of cpDNA. Both data revealed that the distribution of genetic variation followed an "isolation by distance" model (in cpDNA, R=0.745). Phylogeographic analysis based on the cpDNA network suggests that the present-day west and east areas of H. pogonocalyx is a result of past fragmentation. The differentiation between geographic regions may be ascribed to a long isolation since the formation of the Central Mountain Range five million years ago.
目錄
誌謝…………………………………………………………………………Ⅰ
中文摘要……………………………………………………………………Ⅱ
英文摘要……………………………………………………………………Ⅲ
目錄…………………………………………………………………………Ⅳ
表目錄………………………………………………………………………Ⅵ
圖目錄………………………………………………………………………Ⅶ


第壹章 緒言……………………………………………………………… 1
一、親緣地理的研究………………………………………………………1
二、大安水蓑衣的研究歷史回顧…………………………………………5
三、瀕危物種的保育與其遺傳變異………………………………………9
四、分子技術在探討族群結構的應用………………………………….13
五、研究目的…………………………………………………………….15
第貳章 材料與方法………………………………………………………16
一、研究材料…………………………………………………………… 16
二、實驗方法…………………………………………………………… 18
1. RAPD指紋分析………………………………………………………18
2.葉綠體DNA的非轉譯區間序列………………………………………19
第參章 結果…………………………………………………………… 25
一、 RAPD分子指紋………………………………………………………25
二、葉綠體DNA atpB-rbcL noncoding spacer片段………………… 28
三、大安水蓑衣的親緣地理…………………………………………… 30
第肆章 討論…………………………………………………………… 31
一、大安水蓑衣的遺傳變異…………………………………………… 31
二、大安水蓑衣的族群分化…………………………………………… 32
三、 RAPD指紋技術與葉綠體DNA結果的比較………………………… 34
四、大安水蓑衣的親緣地理模式……………………………………… 37
五、大安水蓑衣的保育………………………………………………… 40
第伍章 結論…………………………………………………………… 41
第陸章 參考文獻……………………………………………………… 42
表………………………………………………………………………… 49
圖………………………………………………………………………… 58
附錄……………………………………………………………………… 73
第陸章 參考文獻

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