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研究生:范文佳
研究生(外文):Wen-Gia Fan
論文名稱:低密度脂蛋白對於白血球或血小板與內皮細胞交互作用的影響
論文名稱(外文):Effects of LDL on Leukocyte- or Platelet- Endothelium Interaction
指導教授:陳洵瑛陳洵瑛引用關係
指導教授(外文):Hsiun-ing Chen
學位類別:碩士
校院名稱:國立成功大學
系所名稱:生理學研究所
學門:醫藥衛生學門
學類:醫學學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:中文
論文頁數:61
中文關鍵詞:內皮細胞動脈粥狀硬化低密度脂蛋白
外文關鍵詞:endothelial cellLDLatherosclerosis
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動脈粥狀硬化是一種慢性的發炎性疾病。過去的研究顯示,血漿中低密度脂蛋白濃度不正常的升高會導致白血球在內皮上滾動及黏著的情形增加,並且內皮上相關的黏著分子表現也會增加。有證據指出當培養的人類血管內皮細胞受到低密度脂蛋白作用之後,細胞內會有短暫鈣離子濃度上升的情形,並且啟動了內皮細胞黏著分子VCAM-1、E-selectin的表現。無論如何,低密度脂蛋白在內皮細胞與白血球或血小板之間的交互作用中扮演何種角色,仍然有許多未知。為了要去研究低密度脂蛋白是否會對此交互作用有影響,我們將低密度脂蛋白作用於培養的人類臍帶靜脈內皮細胞(HUVEC),並且運用扇形流體儀中流體剪力成線性變化的特性,來測量白血球與血小板在內皮黏著的情形。另以免疫染色觀察何種黏著分子參與此反應。
我們的結果顯示1)HUVECs在受到高濃度(1.4mg/ml)的低密度脂蛋白作用後,內皮細胞的型態會改變。2) 受到低密度脂蛋白作用後的HUVECs,在經過我們所測試的各個剪力強度下,白血球黏著的情形都明顯高於控制組;而其中單核球黏著又更為明顯,但是對多形性白血球卻沒有影響。3) 單核球較多形性白血球容易黏著上內皮。4)低密度脂蛋白也會使得HUVECs 的黏著分子P-selectin、 VCAM-1表現量增加。我們得到的結論為:低密度脂蛋白的作用會使得白血球(尤其是單核球)的黏著情形增加,而P-selectin、VCAM-1的表現或許參與了此反應。
Atherosclerosis is a chronic inflammatory disease. Previous studies have indicated that abnormally elevated concentrations of plasma LDL will result in an increase in rolling/adhesion of leukocyte and the expression of the relevant adhesion molecules. The existing evidence suggests that the treatment of native LDL (nLDL) in cultured human vascular endothelial cells will induce calcium transient and trigger vascular cell adhesion molecule-1 (VCAM-1) and E-selectin expression. However, the role of nLDL in the interaction of leukocytes/platelets and endothelial cells is largely unknown. To investigate whether the endothelium- leukocyte or platelet interactions could be altered by nLDL, cultured human umbilical vein endothelial cells (HUVECs) were treated with nLDL. Leukocyte and platelet adhesion to HUVECs was subsequently measured by using a tapered flow chamber that has a linear variation of shear stress across its flow channel. To verify which adhesion molecules are involved, the immunostaining were executed. Our data showed that 1) the pretreatment of high dose of nLDL (1.4mg/ml) would change cell morphology of HUVECs; 2) leukocytes, especially monocyte, but not neutrophil , adhered more readily to nLDL-treated HUVECs than to the control group at all shear stress levels tested; 3) the adhesiveness of monocyte on endothelium was higher than that of neutrophil; 4) the expression of P-selectin and VCAM-1 in HUVECs increased after nLDL pretreatment. We conclude that nLDL pretreatment enhances leukocytes adhesiveness, especially monocyte, and that P-selectin and VCAM-1 may be involved.
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方法 -------------------------------- 17
結果 ---------------------------------30
討論 ---------------------------------35
結論 --------------------------------40
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參考文獻 ----------------------------56
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