雷射光鉗是一項利用光壓特性的研究技術。在此文章中我們用Labview程式，模擬幾何光學的理論計算與環境條件，繪出幾何光學模型的曲線分布圖，用來與實驗比較。再利用852nm、50mW的雷射光對1μm及3μm聚苯乙稀微粒垂直捕捉與U937細胞水平捕捉的實驗，聚苯乙稀微粒的垂直捕捉力會因為聚苯乙稀微粒在樣本空間的位置不同而有所不同。當微粒在靠近樣本空間邊界時所需的捕捉力要比微粒在樣本的中央時所需的捕捉力來的大3-5倍。我們也將雷射光鉗應用在細胞上，當使用U937細胞，細胞的大小約為20μm。雷射光鉗能捕捉住細胞，並將其移動並固定到一定的地方。同時雷射光鉗也能應用在生命科學的研究上，例如利用雷射光鉗研究聚苯乙稀微粒與U937細胞之間的吸附力，或是不同細胞之間的作用力。

Using radiation pressure to manipulate micro-size particle, optical tweezers is a novel tool on studying cell molecular biology. In this thesis, a LabView program was constructed with ray optical model to calculate the radiation pressure difference inside a polystyrene sphere for comparison with the experimental data. A diode laser, 852nm and 50mW, was used to trap the polystyrene beads with 1 and 3 mm in diameter and the U937 living cells. Comparing the vertical trapping force on the polystyrene beads at different distance to the cover slit, we can study the boundary effects on the trapping conditions. The vertical trapping forces required near the glass boundary are 3 to 5 times larger than that they were in the center of the solvent. We also demonstrate the capabilities of trapping an U937 (about 20 mm in diameter) living cell with this tweezers. The trapping force on the cells is much smaller than the polystyrene beads due to their irregularity and the small index of refraction. Manipulation of living cells makes the measurement of single cell interaction and communication possible. Further studies may help us understand the mystery of life.

目錄
中文摘要I
英文摘要Ⅱ
目錄Ⅲ
表目錄Ⅵ
圖目錄Ⅶ
第一章、前言
1-1、發展起因1
1-2、光鉗的歷史1
1-3、光鉗的應用4
第二章、原理
2-1、理論模型6
2-2、RO 模型7
2-2-1、單一細光束的作用力推導9
2-2-2、整道光束的作用力推導12
2-3、力的量測14
2-3-1、垂直力(Vertical Force) 14
2-3-2、水平力(Transverse Trapping Force)15
第三章、實驗儀器
3-1、實驗系統16
3-2、醫工所的系統29
第四章、實驗系統
4-1、雷射光鉗的垂直作用力34
4-1-1、樣品的製作34
4-1-2、實驗注意事項35
4-2、細胞培養常用技術38
4-3、細胞39
第五章、實驗過程與分析
5-1、LabView程式撰寫41
5-2、實驗過程46
5-2-1、實驗的先前準備46
5-2-2、垂直力47
5-2-3、水平力54
5-2-4、醫工所的實驗55
5-2-4-1、水平力55
5-2-4-2、生物、醫學上的用途56
5-2-4-3、生物與物理的結合60
第六章、結論62
附錄一、細胞培養常用技術
附錄二、LabView程式圖
參考文獻

參考文獻[1] A. Ashkin, and J. M. Dziedzic, "Acceleration and trapping of particles by radiation pressure", Phys. Rev. Lett. 24, 156 (1970).[2] A. Ashkin , J. m. Dzieic, "Optical Levitation of Liquid Drops by Radiation Pressure", Science 183, 1073 (1975).[3] A. Ashkin and J. M. Dziedzic, "Observation of Resonances in the Radiation Pressure on Dielectric Spheres", Phys. Rev. Lett. 38, 1351 (1977).[4] A. Ashkin, "Applications of Laser Radiation Pressure", Science 210, 1081 (1980).[5] J. P. Gordon, and A. Ashkin, "Motion of atoms in a radiation trap" Phys. Rev. A 21, 1606 (1980).[6] A. Ashkin, and J. M. Dziedzic, "Optocal levitation in high vacuum", Appl. Phys. Lett. 28, 333 (1976).[7] A. Ashkin, and J. M. Dziedzic, ”Observation of light scattering from nonspherical particles using optical levitation", Appl. Opt. 19, 660 (1980).[8] A. Ashkin, J. M. Dziedzic, J. E. Bjorkholm, S. Chu, "Obserbation of a single-beam gradient force optical trap for dielectric particles ", Opt. Lett. 11, 288 (1986).[9] 黃鈞正、吳崇安、邱爾德，"光學嵌住之理論探討"，物理雙月刊，二十二卷，5期 485 (2000).[10] A. Ashkin, and J. M. Dziedzic, "Optical Trapping and Manipulation of Viruses and Bacteria", Science 235, 1517 (1987).[11] H. Misawa, M. Koshioka, K. Sasaki, N. Kitamura, "Three- dimensional optical trapping and laser ablation of a single polymer latex particle in water", J. Appl. Phys. 70, 3829 (1991).[12] W. H. Wright, G. J. Sonek, and M. W. Berns, "Parametric study of the forces on microspheres held by optical tweezers", Appl. Opt. 33, 1735 (1994).[13] R. Omori, T. Kobayashi, and A. Suzuki, "Observation of a single-beam gradient-force optical trap for dielectric particles in air", Opt. Lett. 22, 816 (1997).[14] M. W. Berns, W. H. Wright, B. J. Tromberg, G. A. Profeta,J. J. Andrews, and R. J. Walter, "Use of a laser-induced optical force trap to study chromosome movement on the mitotic spindle", Proc. Natl. Acad. Sci. USA Cell Biology, 80, 4539 (1989).[15] S. Hénon, G. Lenormand, A. Richert, and F. Gallet, "A New Determination of the Shear Modulus of the Human Erythrocyte Membrane Using Optical Tweezers", Biophys. J. 76, 1145 (1997).[16] C. Reichle, T. Schnelle, T. Müller, T. Leya, G. Fuhr, "A new microsystem for automated electrorotation measurements using laser tweezers", BBA, 1459, 218 (2000).[17] T. T. Perkins, D. E. Smith, R. G. Larson, and S. Chu, Science, 268, 83 (1995).[18] W. H. Wright, G. J. Sonek, and M. W. Berns, "Parametric study of the forces on microspheres held by optical tweezers", Appl. Opt. 33, 1735 (1994).[19] Laser Tweezers Theory, Cell Robotics,Inc. [20] POTICS, Hecht 3rd,Addison Wesley Longman,Inc.(1998).[21] R. Omori, T. Kobayashi, and A. Suzuki, "Observation of a single-beam gradient-force optical trap for dielectric particles in air", Opt. Lett. 22, 816 (1997).[22] www.newport.com[23] www.casix.com[24] www.mellesgriot.com[25] www.sinopt.com[26] www.polysciences.com[27] Microscope Workstation User's Manual, Cell Robotics, Inc. Manual Series[28] www.merck.com.tw[29] 動物細胞株目錄，財團法人食品工業發展研究所[30] 國家衛生研究院 (www.nhri.org.tw/b5/plan/catalogindex.htm)[31] G. R. Froemming, and N. M. O'Brien "U937 cells as a model tostudy the effect of phytochemicals on superoxide anion production", Nutrition Research, 17, 1091 (1997).[32] www.lifetech.com[33] M. W. Berns "Laser Scissors and Tweezers", Sci. Ame. 278, 62 (1998).