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研究生:陳嘉齡
論文名稱:雷射鑷夾捕捉力之量測與分析
論文名稱(外文):Measurement and analysis of the trapping forces of a laser tweezers system
指導教授:徐琅
學位類別:碩士
校院名稱:國立交通大學
系所名稱:電子物理系
學門:自然科學學門
學類:物理學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:中文
中文關鍵詞:雷射鑷夾
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雷射鑷夾具有非接觸性捕捉與操縱微米尺度的小粒子之特性,日益成為分子生物學中量測微小生物的運動力的工具。本論文的目標即在利用影像處理技術架設一套可即時量測雷射鑷夾橫向捕捉力的工作平台。
理論上,在雷射鑷夾橫向捕捉範圍內,其捕捉力與微粒子位移量成近似線性作用力關係,有如一「光學彈簧」。對此,我們設計了一雷射鑷夾橫向捕捉力量測實驗,利用已知的水流黏滯力與未知的雷射鑷夾捕捉力達力平衡狀態,據此量化「雷射鑷夾橫向捕捉力」的大小。同時利用影像處理技術測得「微粒子位移量」與「雷射鑷夾橫向捕捉力」的線性關係,來得到光學彈簧的「彈力係數」,並且校正實驗誤差。
在5個不同的雷射功率捕捉之下,實驗結果與理論曲線趨勢相當近似,而且絕對值都相差0.83倍,其誤差來源可能為水黏滯係數隨溫度改變,只要將實驗結果作一比例轉換的校正,仍可作為定量上的分析。
中文摘要 LLLLLLLLLLLLLLLLLLLLLLLL Ⅰ
英文摘要 LLLLLLLLLLLLLLLLLLLLLLLL Ⅱ
誌謝 LLLLLLLLLLLLLLLLLLLLLLLL Ⅲ
目錄 LLLLLLLLLLLLLLLLLLLLLLLL Ⅳ
圖目錄 LLLLLLLLLLLLLLLLLLLLLLLL Ⅴ
表目錄 LLLLLLLLLLLLLLLLLLLLLLLL Ⅶ
一 介紹 LLLLLLLLLLLLLLLLLLLLLL 1
1.1 雷射鑷夾之發展背景與特色 LLLLLLLLLLLL 1
1.2 研究動機與目標 LLLLLLLLLLLLLLLLL 2
1.3 雷射鑷夾橫向捕捉力量測 LLLLLLLLLLLLL 2
1.4 論文各章節簡介 LLLLLLLLLLLLLLLLL 3
二 原理 LLLLLLLLLLLLLLLLLLLLLL 4
2.1 雷射鑷夾理論模型之介紹 LLLLLLLLLLLLL 4
2.2 幾何光學模型 LLLLLLLLLLLLLLLLLL 5
2.3 電磁波模型 LLLLLLLLLLLLLLLLLLL 10
三 雷射鑷夾捕捉力之量測實驗 LLLLLLLLLLLL 15
3.1 雷射鑷夾捕捉力量測實驗之概念及方法 LLLLLLL 15
3.1.1 概念 LLLLLLLLLLLLLLLLLLLLLL 15
3.1.2 方法 LLLLLLLLLLLLLLLLLLLLLL 17
3.2 雷射鑷夾捕捉力量測實驗之實驗裝置及步驟 LLLLL 18
3.2.1 實驗裝置 LLLLLLLLLLLLLLLLLLLL 18
3.2.2 實驗步驟 LLLLLLLLLLLLLLLLLLLL 20
3.3 雷射鑷夾捕捉力量測實驗之影像分析系統 LLLLLL 20
四 結果與討論 LLLLLLLLLLLLLLLLLLL 22
4.1 比較實驗結果與理論預測結果 LLLLLLLLLLL 22
4.2 光學彈簧之彈力係數計算 LLLLLLLLLLLLL 31
五 結論 LLLLLLLLLLLLLLLLLLLLLL 34
參考文獻 LLLLLLLLLLLLLLLLLLLLLLL 36
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