(54.236.58.220) 您好!臺灣時間:2021/03/06 21:58
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果

詳目顯示:::

我願授權國圖
: 
twitterline
研究生:朱俊良
研究生(外文):Tsun-Liang Chu
論文名稱:DNA配對光學量測理論及實驗
論文名稱(外文):An Optical Transduction for Detection of DNA Hybridization
指導教授:林振德林振德引用關係
指導教授(外文):Jenn-Der Lin
學位類別:碩士
校院名稱:國立交通大學
系所名稱:機械工程系
學門:工程學門
學類:機械工程學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:中文
中文關鍵詞:DNA配對(雜交)DNA裂解(變性)薄膜光學
外文關鍵詞:DNA HybridizationDNA DenaturationThin Film Optics
相關次數:
  • 被引用被引用:0
  • 點閱點閱:223
  • 評分評分:系統版面圖檔系統版面圖檔系統版面圖檔系統版面圖檔系統版面圖檔
  • 下載下載:57
  • 收藏至我的研究室書目清單書目收藏:0
本論文欲建立一光學折射率之量測理論模式,並設計一實際量測之實驗,用以偵測DNA於溶液中之配對情況。在考量一般現有的生物晶片之設計後,我們決定使用一多層膜之結構,其中膜的厚度及排列方式各有不同組合,受測之試樣為其中之一層,將光源入射到此結構,依照各層光學性質的不同,經過多重反射之傳輸過程後,會產生部分反射、部分穿透和部分吸收的效果,當膜的厚度小到某一程度後,干涉效應應被考慮。我們首先推導出多層膜結構之光學反射率及穿透率的公式,用來描述光在不同的光學折射率及厚度的情形下,能量傳輸的結果,再利用最小平方誤差法,來進行光學折射率之逆運算分析。我們將探討不同參數變化對於光反射及光穿透之影響,以期在逆運算分析時,能取得最佳的實驗量測組合。在實驗的部分,我們利用建立的量測理論,偵測含有DNA樣品之試液在不同的濃度及配對程度下之光學折射率,並探討此方法在生物分子定量量測之應用性。關於DNA配對過程中雙鏈DNA之解離,我們採取熱學方式來加以控制。

This thesis purposes to develop an optical transduction for detection of DNA hybridization. After considering existing biosensors, the physical model adopted is a multi-layer structure for the present study. The arrangement of layer sequences and thicknesses might be random, and the testing sample layer is included. Due to various optical properties of layers, there exist different degrees of reflection, transmission and absorption of electromagnetic energy with multiple reflections inside the multi-layer structure. Interference effect should be considered as the layer thickness is small. We firstly derive the expressions for reflectance and transmittance of the multi-layer structure, which describe the energy transport under different combination of optical properties and thicknesses of layers. For inverse estimation of optical properties of a specific layer in the structure, the least squared error method is then utilized. Results of the inverse analysis should lead to an optimal design for the experimental measurements. The optical properties of DNA fragment solution in different degrees of hybridization are then measured in association with above-mentioned analysis. As to the controlling of DNA hybridization degrees, the thermal denaturation is utilized.  

目錄
中文摘要 ………………………………………………………… i
英文摘要 ………………………………………………………… ii
誌謝 ………………………………………………………… iii
目錄 ………………………………………………………… iv
表目錄 ………………………………………………………… vi
圖目錄 ………………………………………………………… vii
符號表 ………………………………………………………… ix
第一章 緒論…………………………………………………… 1
1.1 前言…………………………………………………… 1
1.2 研究背景……………………………………………… 2
1.3 研究目的……………………………………………… 4
第二章 量測理論推導及逆運算法…………………………… 6
2.1 物理模式……………………………………………… 6
2.2 基本薄膜光學理論…………………………………… 6
2.3 反射率和穿透率之推導……………………………… 9
2.4 逆運算方法…………………………………………… 11
第三章 實驗方法……………………………………………… 14
第四章 結果與討論…………………………………………… 18
4.1 反射率及穿透率之直接解…………………………… 18
4.2 逆運算結果分析……………………………………… 20
4.3 實驗量測結果………………………………………… 22
第五章 結論…………………………………………………… 27
參考文獻 ………………………………………………………… 28
表 ………………………………………………………… 30
圖 ………………………………………………………… 32

1.Junhui Zhai, Hong Cui and Ruifu Yang, “DNA Based Biosensors,” Biotech. Adv., Vol. 15, No. 1, pp. 43-58, 1997.
2.M. Alvarez-Icaza, H. M. Kalisz, H. J. Hecht, K. D. Aumann, D. Schomburg and R. D. Schmid, “The Design of Enzyme Sensors Based on the Enzyme Structure,” Biosens. Bioelectron. , Vol. 10, pp. 735-742, 1995.
3.M.H.V. Van Regenmortel , Journal of Immunological Method (Special Issue: Uses of Biosensors in Immunology), Vol. 183, pp. 3-175, 1995.
4.I. Karube, “Microbial sensor,” J. Biotechnol. , Vol. 15, pp. 255-265, 1990.
5.K. M. Millan, A. Saraullo and S. R. Mikkelsen, “Voltammetric DNA Biosensor for Cystic Fibrosis Based on a Modified Carbon Paste Electrode,” Anal. Chem., Vol. 66, pp. 2943-2948, 1994.
6.H. Su, P. Williams and M. Thompson, “Platinum Anticancer Drug Binding to DNA Detected by Thickness-shear-mode Acoustic Wave Sensor,” Anal. Chem., Vol.67, pp. 1010-1013, 1995.
7.Y. Okahata, Y. Matsunobu, I. Kunibara, M. Masayuki, A. Murukani and K. Makino, “Hybridization of Nucleic Acids Immobilized on a Quartz Crystal Microbalance,” J. Am. Chem. Soc., Vol. 114, pp. 8229-8300, 1992.
8.P. A. E. Piunno, U. J. Krull, R. H. E. Hudson, M. J. Damha and H. Cohen, “Fiber Optic Biosensor for Fluorometric Detection of DNA Hybridization,” Analytica Chimica Acta, Vol. 288, pp. 205-214, 1994.
9.P. A. E. Piunno, U. J. Krull, R. H. E. Hudson, M. J. Damha and H. Cohen, “Fiber-optic DNA Sensor for Fluorometric Nucleic Acid Determination,” Anal. Chem., Vol. 67, pp. 2635-2643, 1995.
10.A. Thiel, A. Frutos, C. Jordan, R. Corn and L. Smith, “In Situ Surface Plasmon Resonance Imaging Detection of DNA Hybridization to Oligonucleotide Arrays on Gold surfaces,” Anal. Chem., Vol. 69, pp. 4948-4956, 1997.
11.H. Watts, D. Yeung and H. Parkes, “Real-time Detection and Quantification of DNA Hybridization by an Optical Biosensor,” Anal. Chem., Vol. 67, pp. 4283-4289, 1995.
12.A. Hanning, J. Roeraade, J. J. Delrow, R.C. Jorgenson, “Enhanced Sensitivity of Wavelength Modulated Surface Plasmon Resonance Devices Using Dispersion from a Dye Solution,” Sensors and Actuators B, Vol. 54, pp. 25-36, 1999.
13.T. Vo-Dinh, K. Houck, and D. L. Stokes, “Surface-Enhanced Raman Gene Probes,” Anal. Chem. Vol. 66, pp. 3379-3383, 1994.
14.S. S. Yee, J. Homola, G. Gauglitz, Sensors and Actuators B: Chemical, Vol. 54, Issue: 1-2, Jan 25, 1999.
15.R. R. Monaco and F. H. Hausheer, “Binding Site for Ethidium Cation in the Major Groove of B-form DNA,” J. Biomolecular. Struct. Dynamics, Vol. 10, pp. 675-680, 1993.
16.T. Bunemann, “Immobilization of Denatured DNA to Macroporous Supports: II. Steric and Kinetic Parameters of Heterogeneous Hybridization Reactions,” Nucleic Acids Res., Vol.10, pp. 7181-7196, 1982.
17.O. S. Heavens, Optical Properties of Thin Solid Films, Dover Publications, New York, 1991.
18.E. Elizalde and F. Rueda, “On The Determination of The Optical Constants n(λ) and α(λ) of Thin Supported Films”, Thin Solid Films, Vol.122, pp. 45-57, 1984.
19.J. J. Chen, J. D. Lin and L. J. Sheu, “Simultaneous Measurement of Spectral Optical Properties and Thickness of an Absorbing Thin Film on a Substrate”, Thin Solid Films, Vol. 354, pp. 176-186, 1999.
20.R. F. Boyer, Modern Experimental Biochemistry, The Benjamin/ Cummings Publishing Company, 1986.

QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top
系統版面圖檔 系統版面圖檔