在微流道電泳中控制流體的流動主要是以電滲流幫浦來作為驅動的力量，樣品在樣品流道及分離流道的交匯處容易會發生滲漏，發生的原因歸咎於樣品本身的擴散及對流的流體力學效應，而為了增加分離時的分離效率及解析度，抑制樣品的滲漏是重要的，所以需要運用無閥門的流體控制技術將樣品在交匯處的區帶最佳化。
實驗中將會引用文獻中兩種抑制樣品滲漏的技術並作討論，第一種是抑制電壓的方式，在分離樣品的步驟時施加電壓於樣品槽及樣品廢液槽使得流道交匯處的樣品滲漏被抑制住，而這個電壓就是最佳的抑制電壓。另外一種是設計窄的樣品管道運用在交匯處，傳統的樣品進樣是在一個深寬比都一樣的管道中進行，一種新的設計是將樣品管道的寬度及深度變窄，使得在未加抑制電壓的情況下分離結果的表現有所改善。
兩種技術運用在微流道電泳上都具有不錯的抑制樣品滲漏的表現，在抑制電壓的系統中，樣品的分離能夠得到不錯的結果，尤其是fluoresce in及FITC也能夠在900 V/cm時得到完全的分離；在窄的樣品管道的實驗之中，雖然樣品在分離上也有不錯的表現，而且在800 V/cm時就能將fluorescein及FITC完全分離，雖然樣品峰在金屬線熱壓的微流體膠片中的對稱性表現不佳，但是亦能證明其抑制樣品滲漏的功能。

In microchip CE controlling fluid flow by electroosmotic pump is an important issue. Sample leakage easily happened at intersection from sample channel to separation channel. Sample leakage was shown to arise from both diffusive and convective hydrodynamic effects. To use valveless control of fluid flow to set a well—defined sample plug is required. It could be increasing resolution and efficiency for separation.
Two different techniques of inhibiting sample leakage was experienced and discussed in this study. One is pinched voltage system. In separation step, application of potentials to the sample reservoir and sample waster when intersecting channels could inhibiting sample leakage. It is a optimized voltage of pinched voltage. The other one is Narrow Sample Channel form at intersection when floating type in separation step. Conventional injector with uniform channel deepth and width. A novel injector design using reduced sample channel deepth and width to improve separation performance.
For two different techniques for inhibiting sample leakage were shown well in microchip CE. In pinched voltage system, FITC and fluorescein could be separated completely when separation electronic field of 900 V/cm. In Narrow Sample Channel form, last two of laser dyes could be separated in the same conditions and separation electronic field down to 800 V/cm. Even though symmetry of peak was not good when microchannel was made by hot embossing with different size of metal line.

目 錄
中文摘要 ………………………………………………………………… i
英文摘要 ………………………………………………………………… ii
誌謝 ………………………………………………………………… iii
目錄 ………………………………………………………………… iv
表目錄 ………………………………………………………………… vi
圖目錄 ………………………………………………………………… vii
一、 緒論…………………………………………………………… 1
1.1 前言…………………………………………………………… 1
1.2 生物晶片的種類……………………………………………… 2
1.2.1 檢測型晶片…………………………………………………… 2
1.2.2 處理型晶片…………………………………………………… 3
1.3 研究動機及背景……………………………………………… 4
1.4 相關資訊……………………………………………………… 5
二、 毛細管電泳的及微流道電泳的簡介………………………… 10
2.1 毛細管電泳的介紹…………………………………………… 10
2.1.1 毛細管電泳的發展…………………………………………… 10
2.1.2 毛細管電泳的原理…………………………………………… 11
2.1.3 分離方式的種類……………………………………………… 13
2.1.4 偵測方式的種類……………………………………………… 15
2.2 微流道電泳的介紹……………………………………… 17
2.2.1 微流道電泳的起源……………………………………… 17
2.2.2 微流道電泳的應用上的分類…………………………… 18
2.2.3 偵測方式的種類……………………………………………… 19
2.2.4 各類製程的簡介……………………………………………… 21
三、 實驗的架構…………………………………………………… 30
3.1 軟體的撰寫…………………………………………………… 30
3.1.1 Lab-View的介紹…………………………………………… 30
3.1.2 撰寫的目的…………………………………………………… 30
3.1.3 主程式概念的介紹…………………………………………… 30
3.2 硬體的設備…………………………………………………… 32
3.2.1 主要儀器的介紹……………………………………………… 32
3.2.2 儀器架設……………………………………………………… 34
3.3 膠片製作……………………………………………………… 34
3.3.1 線壓方式……………………………………………………… 34
3.3.2 軟性蝕刻……………………………………………………… 35
3.4 基材簡介……………………………………………………… 36
3.5 藥品…………………………………………………………… 39
四、 抑制樣品滲漏之研究………………………………………… 52
4.1 實驗的理由及目的…………………………………………… 52
4.2 抑制電壓於微膠片電泳……………………………………… 54
4.2.1 基本架構的設計……………………………………………… 54
4.2.2 實驗條件……………………………………………………… 55
4.2.3 其他儀器條件………………………………………………… 55
4.2.4 膠片規格……………………………………………………… 56
4.3 樣品注入流道大小於微膠片電泳…………………………… 56
4.3.1 實驗條件……………………………………………………… 56
4.3.2 其他儀器條件………………………………………………… 56
4.3.3 膠片規格……………………………………………………… 57
五、 結果與討論…………………………………………………… 58
5.1 抑制電壓對樣品滲漏的影響………………………………… 58
5.1.1 無抑制電壓的效應…………………………………………… 58
5.1.2 抑制電壓的使用……………………………………………… 58
5.1.3 抑制電壓的判定……………………………………………… 59
5.1.4 過量抑制電壓的效應………………………………………… 59
5.1.5 最佳抑制電壓的效應………………………………………… 60
5.1.6 無法施加小於最佳抑制電壓的原因………………………… 60
5.1.7 多種分析物時，無抑制電壓及最佳抑制電壓的差異……… 61
5.1.8 不同分離電場時，最佳抑制電壓的變動性………………… 62
5.1.9 不同電場下所對應的分離效率……………………………… 62
5.1.10 不同抑制電壓時，電流值監測的重要性…………………… 63
5.2 樣品流道寬窄對於抑制樣品滲漏之影響…………………… 64
5.2.1 相同的流道寬度……………………………………………… 64
5.2.2 以PET為材質時，製作交匯處截面積不同的正交的微流道 65
5.2.3 以PDMS為材質時，製作交匯處寬度不同的正交的微流道 66
5.3 其他影響樣品滲漏之因素…………………………………… 68
5.3.1 高電壓儀及繼電器的操作…………………………………… 68
5.3.2 膠片流道設計………………………………………………… 69
5.3.3 焦耳熱的抑制………………………………………………… 70
5.3.4 微流道膠片貼合……………………………………………… 70
六、 文獻參考……………………………………………………… 83

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