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研究生:韓淳如
研究生(外文):Chun-Ju Han
論文名稱:使用含界面活性劑之冷媒R134a於逆流層析儀萃取親水性物質
論文名稱(外文):Application of Surfactant-Containing Refrigerant R134a to Extract Hydrophilic Compounds in Countercurrent Chromatography
指導教授:余艇
指導教授(外文):Tiing Yu
學位類別:碩士
校院名稱:國立交通大學
系所名稱:應用化學系
學門:自然科學學門
學類:化學學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:中文
論文頁數:76
中文關鍵詞:逆流層析反微胞冷媒R134a親水性物質
外文關鍵詞:countercurrent chromatographyreversed micelles1112-tetrafluoroethaneHydrophilic Compound
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本研究嘗試將含界面活性劑之冷媒R134a(1,1,1,2-Tetrafluoroethane)應用於高速逆流層析儀(High Speed Countercurrent Chromatography)萃取親水性物質,以冷媒R134a取代反微胞萃取法中所使用的有機溶劑,主要是因液態R134a在常溫常壓下為氣體,可與萃取物直接分離,無須額外的濃縮步驟;而高速逆流層析儀提供萃取液R134a與水樣品之間充分的混和,提高萃取效益。在克服高速逆流層析管柱末端壓力降的問題之下,我們以含陽離子型界面活性劑TOMAC(1,1,1,2-trioctylmethylammonium chloride)的液態R134a作為動相,成功的萃取水溶液中的甲基橙,並可直接在管柱末端由R134a釋放,收集濃縮於小量的收集液中。在動相為含2%陽離子界面活性劑TOMAC的17bar R134a萃取甲基橙水溶液時,回收率估計約為80%。由於逆流層析儀管柱耐壓的問題,無法繼續提高壓力,使得目前的儀器系統無法萃取蛋白質。

We try to use surfactant-containing refrigerant R134a (1,1,1,2-tetrafluoro-ethane) to extract hydrophilic matters in High-Speed Countercurrent Chromatography (HSCCC) in this study. Because R134a can separate with extracts without any
concentration after degassing under ambient condition , it is here in use to replace organic solvent in reversed micelle extraction. Complete mixture of R134a and aqueous solution in HSCCC enhaces extraction efficiency. After pressure decreasing at the end of column in HSCCC is overcome, we extract methyl orange from aqueous solution successfully with liquid R134a containing cationic surfactant, trioctylmethylammonium chloride(TOMAC), and concentrate it in small volume of collecting liquid after releasing R134a at the end of column. When using R134a under prsssure,17bar, containing TOMAC which volume concentration is 2% to extract methyl orange from aqueous solution, recovery is estimated to be 80% .We cannot extract proteins in this apparatus due to the deficiency of prssure endurance of column in HSCCC.

目錄
中文摘要…………………………………………………………i
英文摘要…………………………………………………………ii
誌謝………………………………………………………………iii
目錄………………………………………………………………iv
表目錄……………………………………………………………vii
圖目錄……………………………………………………………viii
第一章 緒論…………………………………………………… 1
第二章 研究背景與理論介紹………………………………… 3
2.1 界面活性劑介紹……………………………………… 3
2.1.1 界面活性劑的分類……………………………… 3
2.1.2 界面活性劑的性質與應用……………………… 5
2.2 反微胞萃取法……………………………………………6
2.2.1簡介…………………………………………………6
2.2.2反微胞的鑑定………………………………………7
2.2.3萃取程序……………………………………………7
2.2.4反微胞萃取的機制…………………………………8
2.2.5影響反微胞萃取效率的變因………………………8
2.2.6反微胞萃取法的其他發展…………………………12
2.3環保冷媒R134a……………………………………………12
2.3.1新冷媒R134a的性質……………………………… 13
2.3.2環保冷媒R134a與超臨界流體二氧化碳的比較與應... 14
2.4逆流層析儀………………………………………………… 16
2.4.1簡介………………………………………………………… 16
2.4.2起源與發展………………………………………………… 16
2.4.3理論基礎…………………………………………………… 17
2.4.4儀器系統的基本結構……………………………………… 18
2.4.5反扭轉機構………………………………………………… 19
2.4.6影響靜相滯留的因素……………………………………… 21
2.4.7逆流層析的應用…………………………………………… 22
第三章 實驗
3.1試藥……………………………………………………… 24
3. 1. 1藥品……………………………………………… 24
3. 1. 2溶劑……………………………………………… 25
3.2 儀器設備…………………………………………………26
3.3實驗步驟………………………………………………… 27
3. 3. 1含界面活性劑之R134a萃取蛋白質………………27
3. 3. 2以高壓樣品槽觀測蛋白質的溶解……………… 29
3. 2. 3含界面活性劑之R134a萃取染料…………………29
第四章 結果與討論……………………………………………… 31
4.1 HSCCC儀器的改善……………………………………… 31
4.2影響靜相滯留的因素…………………………………… 31
4.2.1 注入靜相的體積……………………………………31
4.2.2 HSCCC轉速的控制………………………………… 32
4. 2 .3動相流速的控制………………………………… 32
4.2.4壓力的影響…………………………………………33
4.3以含界面活性劑的R134a萃取靜相水溶液中的蛋白質…33
4.4以含界面活性劑的R134a萃取靜相水溶液中的染料甲基橙… 36
第五章 結論……………………………………………………… 39
參考文獻……………………………………………………… 40

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