(44.192.112.123) 您好!臺灣時間:2021/03/06 06:44
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果

詳目顯示:::

我願授權國圖
: 
twitterline
研究生:王明滄
研究生(外文):Ming-Tsang Wang
論文名稱:探討氧化還原電位作為Clostridiumbutyricum連續產氫之研究
論文名稱(外文):Effect of ORP on hydrogen production by clostridium butyricum ia bubble column bioreactor
指導教授:徐敬衡徐敬衡引用關係
指導教授(外文):Chin-Hang Shu
學位類別:碩士
校院名稱:國立中央大學
系所名稱:化學工程與材料工程研究所
學門:工程學門
學類:化學工程學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:中文
論文頁數:56
中文關鍵詞:氧化還原電位氫氣
外文關鍵詞:hydrogenoxidation reduction potential
相關次數:
  • 被引用被引用:15
  • 點閱點閱:363
  • 評分評分:系統版面圖檔系統版面圖檔系統版面圖檔系統版面圖檔系統版面圖檔
  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:1
摘要
氫氣是替代石化燃料的最好選擇。由於石化燃主要來自石油本身,而其蘊藏量不斷地在減少中,並且使用石油後所造成的環保問題與防治成本也不斷地在惡化與增加中。相對地,氫氣燃燒後的產物是水,不會造成環境的負荷,可謂是最乾淨的燃料。因此,氫氣被科學家認為是取代石油世紀的主要能源。氫氣的使用,目前尚未普及化的主要瓶頸是缺乏重要相關科技之研發,如生產技術、儲存、運輸與應用科技。過去二十多年的研發,已開啟許多不同方式來生產氫氣。科學家在文獻中也強調生物法生產氫氣較物理法和化學法為優。
氧化還原電位 ( Oxidation Reduction Potential,ORP )可以用來量測水溶液中碳、氮、硫、磷以及金屬類之間的氧化還原狀態,所以從1983年迄今,一直被用來作為監測水質狀況以及生物反應趨向的指標,廣泛地應用於廢水處理的監測。
一般而言,進行連續式饋料反應均是以碳源的使用情形來判斷是否進料,本實驗則是利用ORP在產氫反應開始時會下降至低點的特性,進行進料的動作。而根據實驗亦證實此法確實可行。
本實驗分別兩大部分討論。第一部分是進行控制不同pH的厭氧醱酵產氫;二是以最佳產氫pH為操控值,並利用ORP作為進料指標來進行連續式饋料產氫。實驗結果顯示,在控制pH=4.5時的批次厭氧產氫有最大產氫量達4630 ml。而依pH值控制的高低不同,產氫時ORP值之最低點及產氫速度會有所不同,pH值6.5、5.5、5.0及4.5的產氫速度依次為:4.98、7.02、11.31及11.38 ml / min。而進行連續饋料產氫方面,利用ORP降至低點時進料共進料五次,得到約19000ml氫氣。在前三次進料時產氫速度均維持在11.37 ml/min,到第四、第五次才依序減為6.42 ml/min及3.90ml/min。
Hydrogen is considered to be one of the best alternative fuels to petroleum. Most of pollutants of our environment are resulted from widely using petroleum as fuels. Hydrogen is also the cleanest energy because its product is water after burning with oxygen. It is believed that hydrogen will replace the petroleum as the major fuel in the next era.
目錄
摘要……………………………………………………………. Ⅰ
目錄……………………………………………………………. Ⅲ
圖索引…………………………………………………………..Ⅴ
表索引………………………………………………………….Ⅶ
第一章 緖論……………………………………………………1
1-1研究動機…………………………………………………1
1-2研究目的…………………………………………………2
第二章 文獻回顧………………………………………………3
2-1前言………………………………………………………3
2-2燃料電池簡介……………………………………………5
2-3 微生物產氫簡介………….………………………………7
2-4 Clostridium…………………………………………………9
2-5 厭氧微生物產氫機制……………………………………12
2-6 環境因子對菌種產氫的影響……………………………18
2-7 氧化還原電位……………………………………………22
第三章 材料與方法……………………………………………26
3-1 實驗材料…………………………………….……………26
3-1-1 微生物……………………………………………………26
3-1-2 培養基組成………………………………………………26
3-1-3 實驗藥品…………………………………………………26
3-1-4 實驗儀器與設備…………………………………………27
3-2 實驗方法…………………………………………….……29
3-2-1菌種保存…………………………………………………30
3-2-2接種菌體培養……………………………………………30
3-2-3厭氧微生物反應器………………………………………30
3-2-4生長曲線的測定…………………………………………32
3-2-5氣體分析…………………………………………………32
3-2-6葡萄糖的分析……………………………………………33
3-2-7代謝酸的分析……………………………………………34
3-3 有機酸對C. butyricum產氫的影響…………………….35
3-4 pH值對C. butyricum產氫的影響………………………35
3-5 連續式饋料產氫……………………..…………………...35
第四章 結果與討論……………………………………………..36
4-1有機酸對C.butyricum產氫之影響………………………36
4-2無pH控制之批次厭氧醱酵……………………………...37
4-3控制pH值之批次厭氧醱酵……………………………...38
4-3-1 pH值對菌體生長的影響………………………………..41
4-3-2 ORP與產氫之關係………………………………….…..42
4-3-3 代謝酸與產氫的影響……………………………….…...45
4-3 連續式饋料厭氧產氫醱酵…………………………..……47
第五章 結論…………………………………………..………...49
參考文獻………………………………………………..………...51
參考文獻王前淇 (1995), “ ORP對固定化污泥反應槽操作之影響”, 國立清華大學化學工程研究所碩士論文白明德, “厭氧生物產氫機制與操作策略之研究”, 成功大學研究所碩士論文, 1999, 台南。許駿發, “工業技術人才培訓計畫講義─高溫丁醇發之理論與應用”, 經濟部工業局, 1998, 台北。Allan E. K., “Distribution and Activity of Microorganisms in Lakes;Effect of Physical Processes. “,Ford Y. E. eds. , pp.47-68, Aquatic Microbiology., Blackwell Scientific Publications, Inc., 1993, Boston.Brosseau, J. D. and Zajic, J. E.,” Hydrogen gas production with Citrobacter intermedius and Clostridium pasteurianum.”, J. Chem. Tech. Biotechnol., 32 496, 1982. Charpentier, J., Martin, G., Wacheux, H. and Gilles, P., (1998), “ ORP regulation and activated sludge:15 years of experience.” ,Wat. Sci. Technol., 38(3):197-208Gest, H. and Peck, H. D., Jr., “A study of the hydrogenlyase reaction with systems derived from normal and anaerobic coli-aerogenes bacteria.”, J. Bacteriol., 70, 326, 1955Gerhard G., “ Bacterial Metabolism .“ , Spring-Verlag New York, pp. 208-282, 1986.Gray, C. T. and Gest, H., “Biological formation of moleculat hydrogen.”, Science, 148, 186, 1965Heydrickx M., Vos De P., Thibau B., Stevens P. and Ley De J. ,” Effect of Various External Factors on the Fermentative Production of Hydrogen Gas from Glucose by Clostridium butyricum Strains in Batch Culture .“ , System. Appl. Microbiol., Vol. 9 pp. 163-168, 1987.Heydrickx M., Vos De P., Vancanneyt M. and Ley De J.,“ The fermentation of Glycerol by Clostridium butyricum LMG 1212t2 and 1213t1 and C. pasteurianum LMG 3285.”, Appl. Microbiol. Biotechnol., Vol. 34, pp. 637-642, 1991.Johns, A. T., “The mechanism of propionic acid formation by Veillonella gazogenes.”, J. Gen. Microbiol., 5, 326, 1951.Johns, A. T. and Barker, H. A., “Methane formation, fermentation of ethanol in the absence of CO2 by methanobacillus omelianskii.”, J. bacterial., 80, 837, 1960.Joseph S. T. and Eva R. K ., “ Intracellular Conditions Required for Initiation of Solvent Production by Clostridium acetobutylicum.”, Appl. and Environ. Microbiol., Vol.52, No.1, pp.86-91,1986.Jun M. Y., Kim S. K. and Kim S.Y. ,“ Riboflavin-Sensitized Phoooxidation of Ascorbic Acid;Kinetics and Amino Acid Effects .”, Food Chemistry, Vol. 53, No. 4, pp.397-403, 1995.Kataoka N., Miya A. and Kiriyama K., “ Studieson Hydrogen Production by Continuous Culture System of Hydrogen-Producing Anaerobic Bacteria .“, Wat. Sci. Tech., Vol. 36, pp. 41-47, 1997.Karube, I., Matsunaga, T., Tsuru, S., and Suzuki, S., “Continuous hydrogen production by immobilized whole cells of Clostridium butyricum.” , Biochim biophys. Acta, 444 , 338 , 1976.Karube I., Urano N., Matsunaga T. and Suzuki S., “ Hydrogen Production from Glucose by Immobilized Growing Cells of Clostridium butyricum.”, Eur. J. Appl. Microbiol. Biotechnol., Vol. 16, pp.5-9, 1982.Koch, F. A. and Oldham, W. K. (1985) , “ Oxidation-reduction potential- a tool for monitoring , control and optimization of biological nutrient removal systems.” , Wat. Sci. Technol., 17:259-281Laurence G., Christian C., Isabel V. and Philippe S. ,“ Regulation of Metabolic Shifts in Clostridium acetobutylicum ATCC 824.” , FEMS Microbiology Reviews, Vol. 17, pp. 287-297, 1995.Lawier, A. , ”Walker Bill to boost hydrogen sparks democratic grumbling .”, Science, 267, 613, 1995.May, P. S., Blanchard, G. C., and Foley, R. T., “Biochemical hydrogen generators:18th Annual Proceedings Power Sources Conferences.”, 1964, May 19-21Mitsui, A. In.,“Solar-Hydrogen Energy System.”, Pergamon, Oxford and New York,p.171,1979.Pakes, W. C. C. and Jollyman , W . H., “The bacterial decomposition of formic acid into CO2 and H2 .”, J. Chem, Soc., 79, 386, 1901Pourbaix, M. (1963), “ atlas dequilibres electrochimiques.”, Gauthiers — Villars et Cie, Editeur-Imprimeur-Libraire, 55, Quai des Grands Augustin, Paris.Rheinheimer G., “ The Influence of Environmental Factors on the Development of Microorganisms.”, Rheinheimer G. eds., Aquatic Microbiology 4th ed., pp.111-147, Baffins Lane, 1992, England.R. Nandi and S. Sengupta ,“Microbial Production of Hydrogen: An Oveview.”, Rohrback, G. H., Scott, W. R., and Canfield, J. H., in proceedings of the 16th Annual Power Sources Conference, 18, 1962.Suzuki, S., Karube, I., Matsunga, T., and Kuriyama, S.,” Biochemical energy conversion using immobilized whole cells of Clostridium butyricum.”, Biochimie, 62, 353, 1980.Suzuki, S., Karube, I., and Matsunaga, T., “Application of a biochemical fuel cell to wastetaters.”, Biotechnol. Bioeng. Symp. No. 8 , 501, 1978.Taguchi, F., Chang, J. D., Mizukami, N., Saito-Taki, T., Hasegawa, K., and Morimoto, M., “Isolation of a hydrogen productionbacteria, Clostridium beijerinckii strain AM 21B from termites.”, Can. J. Microbiol., 39, 726, 1993.Taguchi , F., Hasegawa , K., Saito-Taki, T., and Hara, K.,” Simultaneous production of xylanase and hydrogen using xylan in batch culture of Clostridium sp.”, strainX53, J. Ferment. Bioeng., 81, 178, 1996Taguchi, F., Mizukami, N., Hasegawa, K., Hasegawa, K., and Saito-Taki, T.,” Direct conversion of cellulosic materials to hydrogen by Clostridium sp. Strain no. 2.”, Enzyme Microbiol. Technol., 17, 147, 1995.Taguchi, F., Mizukami, N., Saito-Taki , T., and Hasegawa , K.,”Hydrogen production from continuous fermentation of xylose during growth of Clostridium sp. Strain no. 2, Can. J. Microbiol.”, 41, 536, 1995.Taguchi , F., Yamada , K., Hasegawa , K., Taki-Saito, T., and Hara, K., “Continuous hydrogen production by Clostridium sp. Strain no. 2 from cellulose hydrolysate in an aqueous two-phase system.”, J. Ferment. Bioeng., 82, 80, 1996.Thauer, R. K., Jungermann, K., and Decker, K.,” Energy conservation in chemotrophic anaerobic bacteria, Bacteriol.” , Rev.,41, 100, 1977.Twarog, R. and Wolfe, R. S., “Role of butyryl phosphate in the energy metabolism of Clostridium tetanomorphum.”,J. Bacteriol., 86, 112, 1965Wareham, D. G., Hall, K. J. and Mavinic, D. S. (1993) , “ Real-time control of aerobic-anoxic sludge digestion using ORP.”,J. Environ. Engineer., 119(1):120
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top
系統版面圖檔 系統版面圖檔