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研究生:許峰銘
研究生(外文):Feng-Ming Hsu
論文名稱:表面電漿共振影像系統於DNA微陣列雜交探測
論文名稱(外文):surface plasmon resonance image system for DNA micro-array hybridization
指導教授:陳顯禎
指導教授(外文):S.-J. Chen
學位類別:碩士
校院名稱:國立中央大學
系所名稱:機械工程研究所
學門:工程學門
學類:機械工程學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:中文
論文頁數:67
中文關鍵詞:表面電漿影像系統DNA微陣列相移式干涉術Mach-Zehnder 干涉儀
外文關鍵詞:DNA hybridizationphase+-shift interometryMAch-Zehnder interferometerSPR imagingbiomolecular interaction analysis
相關次數:
  • 被引用被引用:7
  • 點閱點閱:155
  • 評分評分:
  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:0
表面電漿共振影像儀(surface plasmon resonance image system,SPR image system)為利用相位移干涉術(phase shift interferometry,PSI)來檢測微陣列(micro-array)生物分子在固體與液體或固體與氣體界面發生交互作用,所造成界面上之介電常數或厚度微小改變時,反射光波的相位於空間上變化情形,可在無須對生物分子做任何的標記下(labeling),分析生物分子間的交互作用(bio-molecular interaction analysis,BIA)。由於SPR影像系統具有快速地、靈敏度高以及大量平行篩檢(high-throughput screening)等優點,可預期將被廣泛應用在很多生物分子診斷的領域上,諸如抗原與抗體之交互作用、蛋白質分子的非特定吸附、薄膜與蛋白質間交互反應或DNA雜交等。
本論文提出一有別於的其他SPR影像系統的檢測方法,此方法以改良式的Mach-Zehnder相位移干涉術為主,配合五步相位還原演算法來觀測共振角下,反射光波之空間相位因表面電漿共振特性改變而造成的相位飄移(phase drift)情形。
系統目前的共振角搜尋可精準到 度,共振相位計算穩定達 ,可靈敏地量測到折射率變化量為 。配合程式的撰寫,可以即時觀測到二維的微小相位變化趨勢。
Surface plasmon resonance image system was developed to detected interactions between liquid and solid by bio-molecuar microarray or distinguish different gases. This paper propose a different method from other SPR image system. We used the method of phase shift interferometry (PSI) and the structure was base on modified Mach-Zehnder interferometer. With the program was done, we could analysis the real-time distribution of spatial phase of reflective wave resulted from the condition of SPR changed. This system, at present, had the feature to distinguish the difference of refraction index to the level of locally. Therefore, we predict it could apply to high- throughput detection of bio-interaction.
摘要A
ABSTRACTII
誌謝辭III
目錄IV
圖目錄VII
表目錄X
第一章 緒論1
1-1前言1
1-2文獻回顧2
1-3研究動機5
1-4研究方法5
1-5論文架構6
第二章 光激發之表面電漿波7
2-1表面電漿波之色散關係式7
2-2表面電漿波的激發12
2-3 KRETSCHMANN組態下SPR反射率及相位推算15
2-4 以LORENTZIAN曲線描述SPR共振曲線19
第三章 相位移干涉術22
3-1相位跳躍22
3-2光之干涉23
3-3相移式干涉術28
第四章 表面電漿共振影像儀之研製37
4-1表面電漿共振感測器設計37
4-2表面電漿共振影像儀之設計44
4-3人機介面程式開發52
第五章 實驗結果與討論54
5-1實驗器材及說明54
5-2實驗準備、操作及注意事項55
5-3實驗結果57
5-4實驗討論61
第六章 結論64
參考文獻65
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