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研究生:陳志杰
研究生(外文):Chi-Ja Chen
論文名稱:福木對金黃葡萄球菌之抗菌活性成份
論文名稱(外文):ANTIBACTERIAL COMPONENT OF GARCINIA SUBELLITICA LEAVES AGAINST PENICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS
指導教授:楊玲玲楊玲玲引用關係陳立耿
指導教授(外文):Ling-Ling Yang Ph.D.Lih-Geeng Chen Ph.D.
學位類別:碩士
校院名稱:國立嘉義大學
系所名稱:生物科技研究所
學門:生命科學學門
學類:生物科技學類
論文種類:學術論文
論文出版年:2003
畢業學年度:90
語文別:中文
論文頁數:40
中文關鍵詞:抗藥性金黃色葡萄球菌福木Morelloflavone協同作用
外文關鍵詞:drug-resistantStaphylococcus aureusGarcinia subelliticamorelloflavonesynergy
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  • 被引用被引用:1
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金黃葡萄球菌(Staphylococcus aureus)是主要的院內感染源,由於抗生素之濫用其抗藥性也愈來愈嚴重,特別是MRSA (methicillin-resistant Staphylococcus aureus),因此新抗生素的開發成為醫藥界迫切的需求。從天然物開發新藥是新的趨勢,本研究以瓊脂紙錠擴散法(disc agar diffusion method)篩選二十種中草藥對金黃葡萄球菌的抗菌活性,並將具有抗菌活性之藥材繼續進行其活性成份之分離之研究。福木(Garcinia subellitica)是其中最具開發潛力的中草藥之一。福木甲醇萃取物溶於水後以乙酸乙酯進行分配(partition),以抗菌活性追蹤顯示乙酸乙酯層較具活性,乙酸乙酯層再依序以Diaion HP-20 、Silica gel 、ODS 等膠體進行管柱層析,分離出主要的抗菌成分morelloflavone,其結構屬於biflavone類,此化學結構經紫外線吸收光譜、核磁共振光譜及質譜儀分析確認之。Morelloflavone最小抑菌濃度(MIC)為256μg/ml。此外其在低於其MIC濃度時與ampicillin產生協同作用,可降低ampicillin對penicillin抗藥性金黃葡萄球菌(ATCC14154)的最小抑制濃度,而且其協同作用與劑量呈現依存性關係。本研究從天然物中尋找出具抗菌活性物質,與目前使用之抗生素協同作用,可減少抗生素的使用量並降低抗生素的副作用,可開發成為治療MRSA感染的新天然藥物。
Key word: 抗藥性、金黃色葡萄球菌、福木、Morelloflavone、協同作用。

Methicillin-resistant Staphylococcus aureus (MRSA) has gradually become a critical pathogenic source of nosocomial infections. There is an urgent need to discover new classes of antibiotics from natural products in the stream of developing new medicine. In this study, the antibacterial activities of 20 folk medicine extracts against Staphylococcus aureus were screened by agar diffusion method. Garcinia subellitica (Guttiferae) is the potential plant of those. The methanol extract of Garcinia subellitica was partitioned between H2O and EtOAc. An active compound morelloflavone, a kind of biflavone, was isolated from EtOAc layer by a series of Diaion HP-20 、silica gel 、ODS column chromatography. The structure was identified by UV、 Mass、NMR spectroscopy. Morelloflavone, less than it’s minimal inhibit concentration (256μg/ml) dose-dependently synergies with ampicillin to inhibite the penicillin-resistant S. aureus (ATCC14154), and reduce the dosage and the side-effects. Therefore, an anti-Staphylococcus aureus component from Garcinia subellitica is expected to be a potential phytotherapeutic agent for MRSA infections.
Key word: drug-resistant; Staphylococcus aureus; Garcinia subellitica; morelloflavone; synergy.

目 錄
目錄-----------------------------------------------------I
縮寫表-------------------------------------------------III
表目錄--------------------------------------------------IV
圖目錄 --------------------------------------------------V
中文摘要 -----------------------------------------------VI
英文摘要-----------------------------------------------VII
I. 前言--------------------------------------------------1
II. 材料-------------------------------------------------4
II-1. 實驗用中藥材及藥用植物-----------------------------4
II-2. 試驗菌株-------------------------------------------5
II-3. 儀器-----------------------------------------------5
II-4. 試劑、試藥-----------------------------------------6
II-5. 層析用膠體-----------------------------------------6
II-6. 溶劑-----------------------------------------------6
III. 方法------------------------------------------------7
III-1. 藥材萃取物之製備及預試驗-------------------------7
1.植物材料的萃取-----------------------------------------7
2. 植物萃取物之化學成份預試驗----------------------------7
III-2. 福木抗菌活性成份之分離純化------------------------9
1. 福木葉萃取物之高效液相層析指紋圖譜及活性追蹤----------9
2. 福木葉萃取--------------------------------------------9
3. 福木葉成分之分離純化----------------------------------9
III-3. 抗菌活性試驗-------------------------------------12
1. 菌株之活化-------------------------------------------12
2. 細菌之計數-------------------------------------------12
3. 金黃色葡萄球菌生長曲線-------------------------------12
4.檢測藥物紙錠之製備------------------------------------13
5. 培養液、PBS及培養基之配製----------------------------13
6. 瓊脂紙錠擴散法---------------------------------------13
7. 最小抑制濃度-----------------------------------------14
IV. 結果------------------------------------------------15
V. 討論-------------------------------------------------36
VI. 參考文獻--------------------------------------------38

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