臺灣博碩士論文加值系統

硬骨魚類棲息在水中，易隨著外界環境眾多因子的變化而引起生理的反應，但對鹽度改變造成免疫反應的影響所知較少，本實驗針對鹽度改變（包括長期適應及短期改變）引起三種吳郭魚免疫性蛋白質基因（MHC class I、MHC class II、Ig）以及Hb基因表達的影響做研究，並分別探討其在不同的免疫相關組織中的基因表現。
將廣鹽性的莫三比克吳郭魚（Oreochromis mossambicus），由淡水轉移至25 ppt人工配製的海水中，經過不同的轉移時間（0 h、4 h、8 h、24 h以及2週長期適應）後，將鰓、腎臟、肝臟、脾臟、小腸、肌肉以及血液等組織分別取下，並萃取其total RNA，與上述基因片段標定放射性32P之探針進行雜合反應。結果發現長期海水適應與長期淡水適應的比較，除小腸外，各組織間MHC I、MHC II、Ig及Hb的表現大多長期海水適應者較多。短期海水轉換的實驗中，四種基因在鰓的表現會隨著轉換時間而增加，但在長期適應組的表現量較24 h組為低。腎臟、脾臟及小腸中則出現先降再升的情形，尤其脾臟最為明顯，MHC I、MHC II以及Ig這三種基因在脾臟中皆出現此情形，可能是鹽度急遽改變使細胞活性降低的緣故，而MHC I及Ig基因在小腸中各時間點的表現量呈現不規則的變化。
綜合上述結果，鹽度改變對吳郭魚免疫相關器官中三種免疫性蛋白質基因的表現確實有影響，這個結果將可運用在養殖漁業上提高魚類的免疫能力，降低養殖業者因魚類疾病或養殖過程中各種環境壓力造成之漁獲量的損失，進而提升魚類的經濟效益。

Teleost are susceptible to the environmental changes caused the physiological responses, however, the understanding of immunological responses due to salanity change is rare. This study was made attempt to examine MHC class I, MHC class II, immunoglobulin and hemoglobin expressions due to acute seawater（25 ppt）transfer or seawater-adaptation（2 weeks）, and investigated the tissues distribution of four genes in tilapia. Total RNA extracted from various tissue（gill, kidney, spleen, intestine, muscle and blood）of tilapia after seawater transfer, and was applied for the Northern blotting. The data showed that four genes expressions of all tested tissues except intestine in seawater-adaptation fish were higher than those in freshwater-adaptation fish. During acute seawater transfer, four genes expression of tilapia gill were elevated with respect to increasing transfer time. In addition, the expression patterns of four genes in kidney, spleen and intestine were declined at first and increased lately. Interestingly, the expressions of four genes were lower at 24 h seawater transfer than that at seawater-adaptation. In conclusion, the elevated expressions of change immuno-related genes in tilapia respond to the salanity.

中文摘要
英文摘要
壹、 前言
貳、 實驗設計
參、 實驗材料
肆、 實驗方法
伍、 實驗結果
陸、 討論
柒、 結論
捌、 參考文獻圖目錄
表目錄

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