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研究生:賴慧儒
研究生(外文):Hui-Ju Lai
論文名稱:鹽度急遽變化對吳郭魚免疫相關基因之影響
指導教授:翁慶豐翁慶豐引用關係
指導教授(外文):Ching-Feng Weng
學位類別:碩士
校院名稱:國立東華大學
系所名稱:生物技術研究所
學門:生命科學學門
學類:生物學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:中文
論文頁數:92
中文關鍵詞:免疫反應滲透壓鹽度改變吳郭魚
外文關鍵詞:HbIgMHC
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硬骨魚類棲息在水中,易隨著外界環境眾多因子的變化而引起生理的反應,但對鹽度改變造成免疫反應的影響所知較少,本實驗針對鹽度改變(包括長期適應及短期改變)引起三種吳郭魚免疫性蛋白質基因(MHC class I、MHC class II、Ig)以及Hb基因表達的影響做研究,並分別探討其在不同的免疫相關組織中的基因表現。
將廣鹽性的莫三比克吳郭魚(Oreochromis mossambicus),由淡水轉移至25 ppt人工配製的海水中,經過不同的轉移時間(0 h、4 h、8 h、24 h以及2週長期適應)後,將鰓、腎臟、肝臟、脾臟、小腸、肌肉以及血液等組織分別取下,並萃取其total RNA,與上述基因片段標定放射性32P之探針進行雜合反應。結果發現長期海水適應與長期淡水適應的比較,除小腸外,各組織間MHC I、MHC II、Ig及Hb的表現大多長期海水適應者較多。短期海水轉換的實驗中,四種基因在鰓的表現會隨著轉換時間而增加,但在長期適應組的表現量較24 h組為低。腎臟、脾臟及小腸中則出現先降再升的情形,尤其脾臟最為明顯,MHC I、MHC II以及Ig這三種基因在脾臟中皆出現此情形,可能是鹽度急遽改變使細胞活性降低的緣故,而MHC I及Ig基因在小腸中各時間點的表現量呈現不規則的變化。
綜合上述結果,鹽度改變對吳郭魚免疫相關器官中三種免疫性蛋白質基因的表現確實有影響,這個結果將可運用在養殖漁業上提高魚類的免疫能力,降低養殖業者因魚類疾病或養殖過程中各種環境壓力造成之漁獲量的損失,進而提升魚類的經濟效益。
Teleost are susceptible to the environmental changes caused the physiological responses, however, the understanding of immunological responses due to salanity change is rare. This study was made attempt to examine MHC class I, MHC class II, immunoglobulin and hemoglobin expressions due to acute seawater(25 ppt)transfer or seawater-adaptation(2 weeks), and investigated the tissues distribution of four genes in tilapia. Total RNA extracted from various tissue(gill, kidney, spleen, intestine, muscle and blood)of tilapia after seawater transfer, and was applied for the Northern blotting. The data showed that four genes expressions of all tested tissues except intestine in seawater-adaptation fish were higher than those in freshwater-adaptation fish. During acute seawater transfer, four genes expression of tilapia gill were elevated with respect to increasing transfer time. In addition, the expression patterns of four genes in kidney, spleen and intestine were declined at first and increased lately. Interestingly, the expressions of four genes were lower at 24 h seawater transfer than that at seawater-adaptation. In conclusion, the elevated expressions of change immuno-related genes in tilapia respond to the salanity.
中文摘要
英文摘要
壹、 前言
貳、 實驗設計
參、 實驗材料
肆、 實驗方法
伍、 實驗結果
陸、 討論
柒、 結論
捌、 參考文獻圖目錄
表目錄
1.Dixon B., Stet. R.J. The relationship between major histocompatibility receptors and innate immunity in teleost fish. Dev. Comp. Immunol. 25:683-699, 2001.

2.Köllner B., Wasserrab B., Kotterba G., Fischer U. Evaluation of immune functions of rainbow trout(Oncorhynchus mykiss)--how can environmental influences be detected? Toxico. 131: 83-95, 2002.

3.Barton B.A., Zitzow R.E. Physiological responses of juvenile walleyes to handling stress with recovery in saline water. Prog. Fish-Cult. 57: 267-276, 1995.

4.Buchman K., Østergaard L., Glaman J. Affinity purification of antigen-specific serum immunoglobulin from the European Eel ( Anguilla anguilla ).Scan. J. Immunol. 36: 89-97, 1992.

5.Pohajdak B., Dixon B., Stuart R. Immune system. Biochem. mole. Boil. Fishes. 2: 191-205, 1993.

6.Brattgjerd S, Evensen O. A sequential light microscopic and ultrastructural study on the uptake and handling of Vibrio salmonicida in phagocytes of the head kidney in experimentally infected Atlantic salmon (Salmo salar L.).Vet Pathol. 33:55-65, 1996.

7.Mcl. C., Evensen Ø. The morphology of the immune system in teleost fishes. Fish and Shellfish Immunol. 9: 309-318, 1999.
8.Dalmo R. A., Ingebrigtsen K., Bøgwald J. Non-specific defence mechanisms in fish, with particular referance to the reticuloendothelial system (REM). J. Fish Diseases. 20: 241-273, 1997.

9.Dijkstra J.M., Köllner B., Fischer U., Aoyagi K., Kuroda A., Ototake M., Nakanishi T. The rainbow trout classical MHC class I molecule Onmy-UBA*501 is N-glycosylated and expressed in similar cell types as mammalian classical MHC class I molecules. Dev. Comp. Immunol. 24, 2000.

10.Günther E., Walter L. The major histocompatibility complex of the rat(Rattus norvegicus). Immunogenetics. 53: 520-42, 2001.

11.Evans D. H. The physiology of fishes. CRC press, New York. 157-176, 1997.

12.Ferguson H. W., Rice D. A. , Lynas, J. K. Clinical pathology of myodegeneration (pancreas disease) in Atlantic salmon (Salmo salar). Vet. Rec. 119: 297-299, 1986.

13.Hatten F., Fredriksen Å., Hordvik I., Endresen C. Presence of IgM in cutaneous mucus, but not in gut mucus of Atlantic salmon, Salmo salar. Serum IgM is rapidly degraded when added to gut mucus. Fish and Shellfish Immunol. 11: 257-268, 2001.

14.Georgopoulou U., Vernier J. M. Local immunological response in the posterior intestinal segment of the rainbow trout after oral administration of macromolecules. Dev. Comp. Immunol. 10: 529-537, 1986.
15.Grace M. F., Manning M.J. Histogenesis of the Lymphoid organs in rainbow trout, Salmo gairdneri. Dev. Comp. Immunol. 4: 255-264, 1980.

16.Gregory W. W. The immunoglobulin genes of fish. Dev. Comp. Immunol. 19: 1-12, 1995.

17.Hordvik I., Thevarajan J., Samdal I., Bastani N., Krossøy B. Molecular cloning and phylogenetic analysis of the Atlantic salmon immunoglobulin D gene. Scan. J. Immunol. 50: 202-210, 1999.

18.Ishiguro H, Kobayashi K, Suzuki M, Titani K, Tomonaga S, Kurosawa Y. Isolation of a hagfish gene that encodes a complement component. EMBO J. 11: 829-37, 1992.

19.Jobling M. Enviromental biology of fishes. Chapman and Hall, London. 211-249, 1995.

20.Kaattari S.L., Irwin M.J. Salmonid spleen and anterior kidney harbor populations of lymphocytes with different B cell repertoires.
Dev Comp Immunol. 9: 433-44, 1985.

21.Karnaky K. J. Jr. Osmotic and ionic regulation. Fish Physiology (eds. Evan, D. H.) CRC Press, New York. 76: 368-378, 1998 .

22.Laurent P. Gill internal morphology. Fish Physiology (eds. Hoar, W. S. and Randall, D. J.) Academic Press, Orlando. XA: 73-184, 1984 .


23.Lin L.Y., Weng C. F. , Hwang P. P. Effects of Cortisol on Ion Regulation in Developing Tilapia (Oreochromis mossambicus) Larvae on Seawater Adaptation. Physiol. Biochem. Zool. 72:397-404, 1999.

24.Lobb C.J., Clem L.W. The metabolic relationships of the immunoglobulins in fish serum, cutaneous mucus, and bile. Dev and J. Immunol. 127:1525-1529, 1981b.

25.Tsuzuki, M.Y., Ogawa K., Strüssmann C.A., Maita M., Takashima F.. Physiological responses during stress and subsequent recovery at different salinities in adult pejerrey Odontesthes bonariensis. Aquqculture. 200: 349-362, 2001.

26.Mazik, P.M., Simco B.A., Parker N.C. Influence of water hardness and salts on survival and physiological characteristics of striped bass during and after transport. Trans. Am. Fish. Soc. 120: 121-126, 1991.

27.McCormick S. D. Effects of growth hormone and insulin-like growth factor I on salinity tolerance and gill Na+,K+-ATPase in Atlantic salmon (Salmo salar):interaction with cortisol. Gen. Comp. Endocrinol. 101: 3-11, 1996.

28.Meseguer J, Lopez-Ruiz A, Garcia-Ayala A. Reticulo-endothelial stroma of the head-kidney from the seawater teleost gilthead seabream (Sparus aurata L.): an ultrastructural and cytochemical study. Anat Rec. 241: 303-309, 1995.

29.Morgan J. D., Sakamoto T., Grau E. G. , Iwama G. K. Physiological and respiratory responses of the Mozambique tilapia (Oreochromis mossambicus) to salinity acclimation. Comp. Biochem. Physiol. 177A: 391-398, 1997.

30.Nuno M.S., Dos Santos J.J. Taverne-Thiele, Andy C. Branes, Willem B. van Muiswinkel, Anthony E. Ellis and Jan H.W.M. Rombout. The gill is a major organ for antibody secreting cell production following direct immersion of sea bass(Dicentrarchus labrax, L.)in a Photobacterium damselae ssp. Piscicida bacterin: an ontogenetic study. Fish and Shellfish Immunol. 11: 65-74, 2001.

31.Pilstrom L. Immunology of fishes. Immunoglobulin. In Handbook of Vertebrate Immunology(P.P. Pastoret, P.Griebel, H. Bazin&A. Govaerts, eds),15-23, 1998.

32.Pedro N. S. R., Trudi T. H., Jan H. W. M. R., Egbert E. and Rene J. M. Stet. Detection of MHC class II transcripts in lymphoid tissues of the common carp (Cyprinus carpio L.). Dev. Comp. Immunol. 19:483-496, 1995.

33.Potts W. T. W., Foster M. A., Rudy P. P. , Howells G. P. Sodium and water balance in the cichild teleost (Oreochromis mossambicus). J. Exp. Biol. 47: 461-470, 1987.

34.Press, C. McL., Evensen, Ø., Reitan, L. J., Landsverk, T. Retention of furunculosis vaccine components in Atlantic salmon Salmo salar L., following different routes of administration. J. Fish Diseases. 19: 215-224, 1996.

35.Peter J. van den Elsen, Sam J. P. Gobin, Marja C. A. J. yan Eggermond, Ad Peijnenburg. Regulation of MHC class I and II gene transcription: differences and similarities. Immunogenetics. 48: 208-221, 1998.

36.Rene J.M.Stet, Brain Dixon, Saskia H.M. van Erp, Marie-Jose C. van Lierop, Pedro N.S. Rodrigues , Egbert Egberts. Inference of structure and function of fish Major Histocompatibility Complex(MHC)molecules from expressed genes. Fish and Shellfish Immunol. 6: 305-318, 1996.

37.Espelid S., Halse M., Solem ST., Jørgensen TØ.. Immunoglobulin genes and antibody responses in the spotted wolfish (Anarhichas minor Olafsen ). Fish and Shellfish Immunol. 11: 399-413, 2001.

38.Stenvik J., Jørgensen T.Ø. Immunoglobulin D(IgD)of Atlantic cod has a unique structure. Immunogenetics. 51: 452-461, 2000.

39.Nakanishi T., Fischer U., Dijkstra J.M, Hasegawa S., Somamoto T., Okamoto N., Ototake M. Cytotoxic T cell function in fish. Dev. Comp. Immunol. 26: 131-139,2002.

40.Wilson M., Brngten E., Miller N.W., Clem L.W., DuPasquier L., Warr G.W. A novel chimeric Ig Heavy chain from a teleost fish shares similarities to IgD. PNAS(USA) 97: 4593-4597, 1997.

41.Zelikoff J.T. Biomarkers of immunotoxicity in fish and other non-mammalian sentinel species: predictive value for mammals? Toxicol. 129: 63-71, 1998.

42.江佳昌。鹽度改變初期肌酸激酶供應鈉-幫浦能量維持吳郭魚體內滲透壓之恆定。臺灣大學漁業科學研究所碩士論文,台北,民國八十九年。

43.李宗翰。吳郭魚鰓表皮MR細胞的形態與功能研究。臺灣大學動物研究所博士論文,台北,民國八十五年。

44.Hwang P. P., Lee T. H., Weng C. F., Fang M. J. , Cho G. Y. Presence of Na+,K+-ATPase in mitochondria-rich cells in yolk-sac epithelium of larvae of the teleost, Oreochromis mossambicus. Physiol. Biochem. Zool. 72: 138-144, 1999.

45.Kultz D. , Jurss K. Biochemical characterization of isolated branchial mitochondria-rich cells of Oreochromis mossambicus acclimated to fresh water or hyperhaline sea water. J. Comp. Physiol. 163B: 406-412, 1993.

46.McCormick S. D. Cellular and Molecular Approaches to Fish Ionic Regulation, In:Fish Physiology (eds. Hoar, W. S., Randall, D. J. and Farrell, A. P.) Academic Press, San Francisco, 14: 285-315, 1995.

47.Loretz C.A. Electrophysiology of ion transport in teleost intestinal cells. Cell. Mol. Appro. Fish Ion. Reg. 25-56, 1995.

48.Marshall W.S. Transport processes in isolated teleost epithelia: Opercular epithelium and urinary bladder. Cell. Mol. Appro. Fish Ion. Reg. 1-23, 1995.

49.Cann A.J: Principles of Molecular Virology. Academic Press, 2nd Edition, 1997.

50.蕭伊珊。水溫、氨-氮及亞硝酸-氮對台灣鮑魚(Haliotis diversicolor supertexta)免疫反應之影響。屏東科技大學熱帶農業研究所碩士論文,屏東,民國九十一年。

51.曾怡婷。內因性因子與外因性因子-氨、亞硝酸及硫酸銅影響白蝦免疫反應。國立海洋大學水產養殖學系碩士論文,基隆,民國九十一年。

52.劉俊宏。溶氧對淡水長臂大蝦血淋巴生理及免疫反應的影響。屏東科技大學熱帶農業研究所碩士論文,屏東,民國九十年。

53.Weng C.F., Chiang C.C., Gong H.Y., Chen M.H., Lin C.J., Huang W.T., Cheng C.Y., Hwang P.P., Wu J.L. Acute changes in gill Na+-K+-ATPase and creatine kinase in response to salinity changes in the euryhaline teleost, tilapia (Oreochromis mossambicus). Physiol Biochem Zool.75: 29-36, 2002.

54.Cann A.J. Principles of Molecular Virology.1997.
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