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研究生:蔡住當
研究生(外文):Tsai Chu-Dang
論文名稱:陰道滴蟲受鐵離子調控基因啟動子的分析
論文名稱(外文):Characterization of an Iron-responsive Promoter in the Protozoan Pathogen Trichomonas vaginalis
指導教授:戴榮湘
指導教授(外文):Tai Jung-Hsiang
學位類別:博士
校院名稱:國防醫學院
系所名稱:生命科學研究所
學門:生命科學學門
學類:生物學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:中文
論文頁數:78
中文關鍵詞:陰道滴蟲啟動子基因調控鐵離子
相關次數:
  • 被引用被引用:1
  • 點閱點閱:195
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  • 下載下載:17
  • 收藏至我的研究室書目清單書目收藏:0
中文摘要
亞鐵離子會影響陰道滴蟲細胞內許多基因的表現以及含鐵離子酵素的活性,本文的研究目的就是要探討陰道滴蟲的黏附蛋白基因(ap65-1)表現受到亞鐵離子濃度調控的機制。
利用DNA短暫轉染技術,來分析ap65-1基因啟動子區域受亞鐵離子濃度調控的DNA序列,發現啟動子區-109/-56的序列與亞鐵離子誘導轉錄活性有關,而-121/-102,-52/-39和initiator element與基礎轉錄活性有關。再以陰道滴蟲細胞核蛋白質對有關的啟動子區調控序列進行結合反應,發現
-95TAACGATAT-87 與轉錄因子MYB-family的DNA 結合位置序列(c/tAACGg/t)相似,而且-95TAACGATAT-87的序列與亞鐵離子誘導轉錄活性有關。啟動子區遠端的T-rich region能與核蛋白質結合並能增強基礎轉錄與誘導轉錄活性的表現。
由以上實驗說明本研究已在陰道滴蟲建立起DNA短暫轉染系統,並且運用此一系統發現ap65-1基因啟動子區的調節序列。未來的研究可利用此一模式探討陰道滴蟲其它基因的轉錄調控機制。

英文摘要
Iron is a key modulator of gene expression in Trichomonas vaginalis. In this study, iron induced gene regulation of T. vaginalis adhesin protein(AP65-1)was investigated.
A transient DNA transfection system was established and for the analysis of ap65-1 promoter activity. The region -109/-56 was found to be critical for iron-induced transcription and -121/-102、-52/-39 and initiator element were found to be important for basal transcription. Nuclear proteins binding sites in these regions were identified by electrophoretic mobility shift assay, was shown to be
-95TAACGATAT-87 and T-rich sequence in the distal promoter region.
According to these results, DNA transient transfection system is usfeul to further study basal and iron-induced transcriptional regulation in T. vaginalis.

正文目錄

正文目錄. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Ⅰ
表目錄. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Ⅱ
圖目錄. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Ⅲ
附錄目錄. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Ⅳ
縮寫表. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Ⅴ
中文摘要. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Ⅵ
英文摘要. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Ⅶ
第一章 緒言. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1
第二章 實驗設計. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8
第三章 結果. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11
第四章 討論. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16
第五章 參考文獻. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .52
附錄

第五章 參考文獻
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