臺灣博碩士論文加值系統

內毒素是細菌細胞壁上的脂多醣體，會造成體循環中的血管舒張，產生敗血症；氣喘病患中，內毒素會引起平滑肌的收縮，引發哮喘。因此，我們探討氣管、肺動脈與胸主動脈等培養平滑肌細胞，對於內毒素反應之差異。實驗結果顯示，內毒素誘導培養細胞上清液中的亞硝酸鹽累積量會隨著作用時間增加(5 ~ 48小時)而增加；氣管平滑肌在100 microgram/ml內毒素作用48小時之後，所得到亞硝酸鹽累積量的濃度為12.03 ± 0.70 microM，顯著小於血管平滑肌的30.02 ±1.68 microM。控制組以及給予內毒素在靜止時期細胞內游離鈣離子的濃度在氣管平滑肌分別是0.589 ±0.009與0.572 ±0.009 (F/Fo)；在肺動脈平滑肌則是0.558 ±0.006與0.573 ±0.007；在胸主動脈平滑肌是0.585 ±0.008與0.582 ±0.007。顯示內毒素並不會改變細胞內鈣離子的濃度，另外在利用三磷酸腺引發的鈣離子釋放，在氣管、肺動脈以及胸主動脈平滑肌中分別為0.47 ±0.041、0.486 ±0.029以及0.527 ±0.027 (ΔF/Fo)，而給予內毒素處理過後，鈣離子釋放量分別為0.697 ±0.033、0.646 ±0.03以及0.618 ±0.028 (ΔF/Fo)，在三種平滑肌細胞中均有顯著的增加。
此研究證實了血管平滑肌細胞在受到內毒素刺激之後，較氣管平滑肌細胞容易產生較多的一氧化氮。細胞內釋放鈣離子的機轉可能受到一氧化氮的調控，並且在氣管與血管平滑肌細胞之間，受到調控的程度不同。

The predominant hemodynamic changes in sepsis and endotoxemia are systemic hypotension and tracheal contraction. Thus we explored the differences among aortic smooth muscle cells of aorta, pulmonary artery, and trachea. In this study, the nitrite accumulation and calcium mobilization (induced by 30 microM ATP), detected by a calcium-sensitive fluorescent dye (fura-2), among cultured tracheal, pulmonary artery, and aortic smooth muscle were evaluated during LPS exposure. And the result indicated that LPS increased nitrite accumulations in supernatant in a time-dependent manner. However, after exposure with LPS (100 microgram/ml) for the nitrite accumulation in TSMCs (12.03 ±0.70 microM) was significantly lower than in PASMCs and ASMCs (30.02 ±1.68 and 30.44 ±2.38 mM). The resting [Ca2+]i in TSMCs, PASMCs and ASMCs were 0.589 ±0.009, 0.558 ±0.006 and 0.585 ±0.008 (F/Fo) respectively. And after LPS treatment the resting [Ca2+]i were 0.572 ±0.009, 0.573 ±0.007 and 0.582 ±0.007. The amplitude of calcium mobilization (peak [Ca2+]i minus resting [Ca2+]i) after LPS treatment was significantly increased from 0.47 ± 0.041 to 0.697 ±0.033, 0.486 ±0.03 to 0.646 ±0.02 and 0.527 ±0.027 to 0.618 ±0.028 (ΔF/Fo) respectively. To conclude, the modulation of intracellular calcium might be influenced by nitric oxide with different degree in vascular and tracheal smooth muscle cells.

目錄………………………………………………………………………I
圖次………………………………………………………………………II
表次………………………………………………………………………III
中文摘要…………………………………………………………………IV
英文摘要…………………………………………………………………VI
緒論………………………………………………………………………1
研究目的…………………………………………………………………6
實驗材料與方法…………………………………………………………7
實驗結果…………………………………………………………………12
討論………………………………………………………………………17
結論………………………………………………………………………22
參考文獻…………………………………………………………………29

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