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研究生:姜蜀海
研究生(外文):Jiang , Shu-Hai
論文名稱:高壓氧對於缺血性中風大白鼠神經細胞存活的影響
論文名稱(外文):The Effect of Hyperbaric Oxygen on Neuron Survival after the Postischemic Stroke in Rats
指導教授:劉江川王順德吳慶祥
指導教授(外文):Liu, Jiang-ChuanWang, Shwun-DeWu, Ching-Hsiang
學位類別:碩士
校院名稱:國防醫學院
系所名稱:生物及解剖學研究所
學門:生命科學學門
學類:生物訊息學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:中文
中文關鍵詞:高壓氧缺血性中風細胞凋亡
外文關鍵詞:TUNEL stainTTC stainMCAO modelHBO
相關次數:
  • 被引用被引用:1
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  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:0
因缺血性缺氧所造成的腦部傷害可能由於腦神經細胞死亡所產生。經由高壓氧治療可以增加溶解於血漿中氧氣含量,因此當暫時性腦缺血事件發生後接受早期高壓氧治療證實可以改善傷害的程度,但高壓氧治療在劑量及方式上是一個重要問題。高壓氧治療機轉可能包含增加周圍細胞供氧量及缺血週邊氧量。先前一些文獻報告高壓氧治療可以減少腦部梗塞面積,腦梗塞機率及缺血性中風運動症狀缺損,然而卻有學者提出晚期治療其結果是有害的。目前高壓氧治療對於細胞層次的機轉仍然不清楚。本實驗利用中大腦動脈阻塞方式,造成大白鼠缺血性中風後,將大白鼠放入高壓氧艙中接受治療,然後先評估大白鼠行為症狀,接著用TTC染色來觀察其腦梗塞面積變化,再利用TUNEL染色來觀察神經細胞凋亡的情形。實驗結果在接受早期高壓氧治療後之大白鼠其大腦皮質有降低細胞凋亡數目,顯示早期高壓氧治療可能有助於增加缺血性中風大白鼠神經細胞存活。

Hypoxic-ischemic brain injury occurred could lead to brain cell death. To increase the amount of oxygen dissolved in blood plasma is by means of hyperbaric oxygen (HBO). HBO can improve the outcome after temporary focal ischemia when the treatment is started early after ischemia. Potential mechanisms of HBO treatment include enhanced oxygen supply to marginally perfused cells and ischemic periphery. Several reports in the literature had shown that hyperbaric oxygen reduced cerebral infarction area in animals. Recent reports showed the HBO therapy can decrease the incidence of infarction and attenuate motor deficits. So we want to know the mechanism of the HBO treatment. We applied the middle cerebral artery (MCA) occlusion model, then placed the rats in the HBO chamber according to the protocol. The rats were evaluated with behavioral neurological test and cerebral infarction area. The apoptosis was also examined with TUNEL stain and counterstained with DAPI. Our data suggest that the early treatment of HBO decrease the apoptotic cells after postischemic stroke in rats. So the early treatment of HBO may enhance the neuron survival after postischemic stroke in rats.

目錄…………………………………………………………… I
圖目錄 …………………………………………………… III
中文摘要…………………………………………………… IV
英文摘要…………………………………………………… V
第一章 緒論………………………………………………….1
第一節 簡介………………………………………………… 1
第二節 缺血性中風造成腦細胞受傷的可能機轉………… 2
第三節 缺血性腦中風動物實驗模式……………………… 5
第四節 高壓氧簡介………………………………………… 6
第五節 高壓氧治療效果的可能機制……………………… 7
第六節 高壓氧治療之副作用……………………………… 8
第七節 高壓氧與腦中風治療……………………………… 8
第八節 實驗目的.................................. 9
第二章 材料與方法…………………………………………10
第一節 大腦中動脈阻塞手術方法…………………………10
第二節 大白鼠運動行為的評估方法………………………10
第三節 TTC染色………………………………………… 11
第四節 組織標本製作…………………………………… 12
第五節 TUNEL染色及 DAPI染色……………………… 12
第六節 資料分析與統計.......................... 13
第三章 實驗結果……………………………………………14
第一節 運動行為評估………………………………………14
第二節 TTC染色結果……………………………………… 14
第三節 TUNEL染色及 DAPI 染色結果…………………… 15
第四章 討論......................................16
第五章 結論……………………………………………… 20
第六章 參考文獻................................ 30

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