臺灣博碩士論文加值系統

以微透析裝置連結高效能液相層析儀分析茶品的四種兒茶素含量，就HPLC系統和微透析系統進行多項變因的探討。層析條件包括動相和層析管柱的選擇；透析條件包括樣品溶液的pH值和添加鹽類濃度、灌注液添加極性修飾劑比例、灌注液流速和pH值及收集時間等影響透析之因素，找出最佳條件。
實驗結果以100%甲醇為灌注液，流速為1.06 μl/min，收集30分鐘條件下，連線HPLC動相為pH 4，10-3M磷酸緩衝水溶液混和氫甲烷，搭配逆向C-8管柱進行梯度沖提，分析主要四種兒茶素。
在最佳層析及微透析條件下分析得回收率為EGC：77﹪、EC：73﹪、EGCG：80﹪、ECG：74﹪；偵測極限為，EGC：0.7ppm、EC：1.2ppm、EGCG：3.2ppm、ECG：0.6ppm。研究並針對市售茶飲進行兒茶素含量的檢測。

The purpose of the study was to analyze 4 catechins, namely epicatechin (EC), epigallocatechin (EGC), epicatechin gallate (ECG) and epigallocatechin gallate (EGCG), in tea drinks by micro-dialysis connected with HPLC. The conditions of HPLC analysis included the stationary phase and the mobile phase were examined. Also the conditions of micro-dialysis which included the pH and the concentration of salt adding of the sample as well as the pH, flow rate, and the modifier of perfusate were examined.
The optimal conditions of the micro-dialysis sampling were 100% methanol as perfusate with the flow rate of 1.06 mL/min and 30 min. collection time. And the optimal conditions of HPLC analysis were C8 reversed phase column with the mobile phase of acetonitrile and pH4, 10-3 M phosphate buffer aqueous solution by gradient condition.
By the optimal conditions, the recoveries of micro-dialysis were 77%, 73%, 80%, and 74% for EGC, EC, EGCG and ECG, respectively. And the detection limites of 0.7, 1.2, 3.2, and 0.6 ppm were found for EGC, EC, EGCG and ECG, respectively, for the system of micro- dialysis connected with HPLC. The commercial tea drinks were also examined by the method and the results are reported.

謝誌­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­Ⅰ
摘要­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­Ⅱ
英文摘要(Abstract) ­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­Ⅲ
目錄­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­Ⅳ
圖次­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­Ⅵ
表次­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­Ⅷ
簡語表­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­Ⅸ
第一章緒論­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­1
一、前言­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­1
二、兒茶素的介紹­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­2
三、微透析的介紹­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­12
四、研究動機­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­22
第二章研究方法­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­24
一、實驗藥品、器材、儀器設備­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­24
二、藥品配製­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­27
三、實驗過程­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­30
四、透析暨層析方法再現性測試­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­36
五、校正曲線的建立­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­37
六、透析回收率的探討­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­37
七、偵測極限的測定­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­37
八、真實樣品的分析­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­38
第三章 結果與討論­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­39
一、液相層析方法的建立­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­39
二、微透析方法的建立­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­47
三、透析暨層析方法再現性的測試­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­62
四、透析回收率的探討­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­62
五、偵測極限的探討­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­62
六、真實樣品的分析­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­66
第四章結論­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­70
第五章參考文獻­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­71

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