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研究生:陳玉樹
研究生(外文):Yu-Shu Chen
論文名稱:鹽分與乾旱逆境對烏山頂泥火山地區植物分佈之影響
論文名稱(外文):Effects of salt and drought stress on plant distribution in Wushanting Mud Volcanic areas
指導教授:廖麗貞廖麗貞引用關係
指導教授(外文):Li-Jen Liao
學位類別:碩士
校院名稱:國立高雄師範大學
系所名稱:生物科學研究所
學門:生命科學學門
學類:生物學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:中文
中文關鍵詞:鹽分逆境乾旱逆境植物分佈烏山頂泥火山
外文關鍵詞:salt stressdrought stressplant distributionWushanting Mud Volcano
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本研究以烏山頂泥火山地區全區優勢植物大花咸豐草(Bidens pilosa L. var. radiata Sch.)以及局部優勢植物鯽魚膽(Pluchea indica (L.) Less.),兩種於烏山頂泥火山地區不同分佈狀況的植物為實驗材料,除野外採樣外,另以不同泥漿比例(體積比)栽種控制組,並於種植一個月後進行乾旱處理,探討鹽分與乾旱逆境對其分佈的影響。
結果顯示,大花咸豐草野外樣品總蛋白質含量隨著旱季的持續略有下降,鯽魚膽則無此現象;脯氨酸的含量,大花咸豐草於不同樣點與不同時間都有變化,而鯽魚膽僅於不同樣點間有較大變化;總蛋白質電泳分析可以發現,大花咸豐草於樣點4到樣點8在77KD、55KD、40KD與26KD附近有明顯條帶出現,鯽魚膽則於50KD與26KD附近各出現一蛋白質條帶;大花咸豐草過氧化酶活性於實驗期間隨著旱季的持續而增加,鯽魚膽也有相同的情形,但不如大花咸豐草明顯;由過氧化酶IEF電泳分析發現,當兩種植物處於高鹽分與乾旱時,酸性的過氧化酶條帶會出現增加與增強的情形。
大花咸豐草於乾旱處理前,不同泥漿比例的控制組,雖然總蛋白質含量變化不大,但高泥漿比率處理組(大於50﹪),脯氨酸含量卻隨著時間大量增加至4000μg/g以上,之後即有植株死亡的現象,低泥漿比率處理組(小於33.3﹪)於乾旱處理後總蛋白質含量大量上升,但脯氨酸的含量並不如預期會於乾旱處理時上升,反而下降;總蛋白質電泳可發現大花咸豐草於前4次採樣期間,總蛋白質電泳的條帶數均呈現增加的情形,以55KD、40KD與26KD附近較為明顯,而鯽魚膽100﹪與75﹪泥漿組,第2次採樣時於26KD附近有另一明顯條帶,第5次採樣時則於30KD附近有另一條帶出現;控制組大花咸豐草過氧化酶活性於高泥漿比率(50﹪以上),明顯高於低泥漿比率(33.3﹪以下),且各處理內過氧化酶活性差可達7倍,鯽魚膽控制組過氧化酶活性的變化,乾旱處理前各處理間差異不大,乾旱處理後,100﹪與75﹪泥漿組過氧化酶活性增加量達40倍以上,而50﹪與25﹪泥漿組過氧化酶活性增加達8倍以上,0﹪泥漿組則僅有2倍;控制組過氧化酶IEF電泳亦發現酸性過氧化酶條帶於高鹽分與乾旱會出現增加與增強的情形。
過氧化酶活性與脯氨酸累積量跟植物的生長以及逆境的抗性有關,大花咸豐草與鯽魚膽,對於逆境的反應並不相同,特別是在過氧化酶活性與脯氨酸累積量方面,兩者有很大的不同其差異可能導致對環境適應上的不同,使得鯽魚膽僅能分佈於高鹽分但較潮濕的環境(泥火山周邊),而大花咸豐草則可以分佈於高鹽環境以外的所有區域。
Abstract
This research is based on two different plants were sampling from the peak of Wushanting Mud Volcanic areas. The plants mentioned in this abstract consist of Bidens pilosa, a widely sprawling plant in the area, and Pluchea indica, a regional dominant plant found in this mountain. Aside from taking the samples from Wushanting, different ratios of mud were added into the planting, and the situation of drought was simulated a month later, so as to analyze the impact of salt and drought on the distribution of these plants.
The result shows that the protein in Bidens pilosa sampled from the wild decreases slightly with the impact of drought, whereas Pluchea indica does not have this phenomenon. The amount of proline in Bidens pilosa alters with place and time, whereas the amount in Pluchea indica varies only with place. The total protein electrophoresis analysis for Bidens pilosa shows that a protein belt appears in places number 4 and 8 at 77KD, 55KD, 40KD, and 26KD. The analysis for Pluchea indica calls for a protein belt in 50KD and 26KD. Both Bidens pilosa and Pluchea indica demonstrate an increase of peroxidase as drought arrives, with the situation more apparent in Bidens pilosa. The results in peroxidase electrophoresis analysis show that when these two plants are situated under saline and water crisis situations, acid peroxidase belts appear to intensify.
Although the total amount of protein for different mud proportions of Bidens pilosa does not alter greatly before the dehydration, the amount of proline in high proportions of mud (above 50%) will augment to over 4000ug/g, then show signs of death. The protein in low mud groups (below 33.3%) increases greatly after dehydration, but the amount of proline decreases, instead of increasing as expected. From total protein electrophoresis, we can discover that the total protein electrophoresis belt in the first 4 sample collection periods, particularly apparent near 55KD, 40KD and 26KD. Pluchea indica 100% and 75% mud groups demonstrated an obvious belt during the second sample collection at 26KD, and another during the fifth sample collection at 30KD. The peroxidase activity in high mud groups (over 50%) of Bidens pilosa is evidently more prominent than in low mud groups (below 33.3%), and it could reach up to 7 times the difference within groups. The peroxidase activity changes within Pluchea indica showed little difference before and after dehydration. After dehydration, the peroxidase of 100% and 75% lava groups increased 40 times the original amount, and the peroxidase activity of 50% and 25% lava groups increased 8 times. As for the 0% group, the increase of only 2 times was found. From the peroxidase IEF electrophoresis, we have discovered that the acid peroxidase belt intensifies with the increase of salt and dehydration.
Peroxidase activity and proline affect the plants’ growth and their ability to resist tough environments, which in turn influences their adaptability of different surroundings. This research illustrates that Bidens pilosa and Pluchea indica show different reactions to difficult environments, particularly in the aspects of peroxidase activity and proline. Pluchea indica survives in highly saline areas (around the mud volcano) whereas Bidens pilosa distributes in non-saline environments.
目 錄
中文摘要.........................I
英文摘要.........................III
壹、前言.......................1
貳、前人研究.....................3
參、材料方法.....................11
一、樣點位置與概況................11
二、氣候概況...................14
三、實驗材料...................14
四、實驗方法...................16
肆、結果.......................22
一、土壤理化性質分析...............22
二、總量蛋白質..................29
三、總蛋白質電泳分析...............33
四、過氧化酶活性測定...............38
五、游離脯氨酸含量測定..............47
六、過氧化酶等電點電泳分析............50
伍、討論.......................58
陸、參考文獻.....................66
陸、參考文獻
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