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研究生:鄧蕙芳
研究生(外文):Huey-Fang Teng
論文名稱:菊花端節酵素基因表現之研究
論文名稱(外文):Studies on Telomerase Gene Expression of Chrysanthemum
指導教授:廖麗貞廖麗貞引用關係
指導教授(外文):Li-Jen Liao
學位類別:碩士
校院名稱:國立高雄師範大學
系所名稱:生物科學研究所
學門:生命科學學門
學類:生物學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:中文
論文頁數:46
中文關鍵詞:telomerase
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中文摘要
菊花是歐美國家三大花卉之一,在台灣及日本則為最重要的切花花卉作物,菊花切花瓶插壽命長,其花色、花型多且變化大,容易進行產期調節,又可以全年栽培。
在菊花栽培上,早期大多數以扦插進行商業繁殖,後來許多研究顯示已可成功地藉由微體繁殖和各種組織的芽體生成(器官生成),以及癒傷組織培養等進行大量增殖。本研究以大黃菊花瓣及無菌苗葉片為材料,花瓣培植體以MS基礎培養基添加10 ppm IAA、10 ppm BA及0.1 ppm kinetin;葉片培植體以全量MS基礎培養基添加5.0 ppm IAA、5.0 ppm BA及0.05 ppm kinetin誘導癒傷組織的生成,結果發現無論在葉片或花瓣均能誘導癒傷組織生成,並成功地生成芽體與再生幼葉,且在葉片培養上的生長分裂速度較花瓣快速。
以含有2﹪cellulase RS、1﹪macerozyme R-10的1/4MS基礎培養基分離黃菊葉肉原生質體與菸草葉肉原生質體,結果發現二者在培養初期之第一次及第二次細胞分裂時,其分裂的速度即有明顯不同,以菸草的分裂速度較快,數目也較多,而黃菊葉片的原生質體在本試驗培養基中僅能持續細胞分裂至小細胞團為止,無法進行分化。
在端節酵素活性的研究方面上,將已分化組織,如葉片及花瓣經培養去分化形成癒傷組織,其端節酵素活性能被活化,且在未分化細胞,如原生質體有高的活性表現,尤其以進入第一次分裂及第二次分裂時的表現量最高。在植物開花時期,其細胞分裂與分化旺盛,端節酵素活性亦再度被活化。因此,可得知端節酵素活性在植物中,並非一直處於高度表現狀態,會因生長階段不同而有所差異。

Abstract
Chrysanthemum is the one of the three major flower harvest in Occident, and it is also the appreciate cut-flower in Taiwan and Japan. The color and shape of Chrysanthemum is enormous variety, and the cut-flower is long-lived. It is easy to adjust the harvest period of Chrysanthemum, and it can plant in the whole year.
In the earlier cultivation of Chrysanthemum, it was used to using cuttings in business. Later, it is succeed to proceed to enormous proliferation in micropropagation, shoots regeneration (organosynthesis ) and callus culture. The materials of this study are petals of Dendranthema grandiflourm and leaves of aseptic seedlings. Callus formation from petals explants were cultured on the medium containing basal MS medium, 10 ppm of IAA, 10 ppm of BA and 0.1 ppm of kinetin;leaves explants were on the medium containing basal MS medium, 5.0 ppm of IAA, 5.0 ppm of BA and 0.5 ppm of kinetin. The result is no matter using leaves or petals, both can induce callus formation, organosynthesis and reborn young leaves. The rate of growth and division in leaves culture is rapider than in the petals culture.
Isolation of protoplasts in mesophyll of Chrysanthemum and tobacco (Nicotiana tobacum L.) by 1/4 MS medium supplemented with 2﹪ cellulase RS, 1﹪ macerozyme R-10. The result is their division ratesss obviously different during the first and second cell division in the early cultivated stage .The division rate and numbers of tobacco are rapider than of chrysanthemums. The protoplasts from chrysanthemum leaves just can grow into small cell groups, but not into differentiation in this experiment.
In the research of telomerase activity, it was activated in the dedifferentiation tissue which is callus culture from leaves and petals. Especially during the period of the first cell division into the second cell division, the telomerase activity of undifferentiating cell, like protoplasts, is more active. During the period of blooming, plant cells divide and differentiate lively, and their telomerases are reactive. The telomerase activity in plants is not still in higher expressional state. It depends on the different growth stage.

目 錄
中文摘要…………………………………………………I
英文摘要…………………………………………………III
目錄………………………………………………………V
壹、前言…………………………………………………1
貳、前人研究……………………………………………3
一、菊花的生物學特性……………………………3
二、菊花的組織培養………………………………4
三、染色體端節與染色體端節酵素………………6
參、材料與方法…………………………………………10
一、實驗材料………………………………………10
二、菊花葉片培養…………………………………15
三、菊花小花花瓣培養……………………………15
四、原生質體的分離、純化與培養………………15
五、細胞萃取液(cell lysate)的製備……………18
六、細胞萃取液蛋白質定量分析…………………19
七、染色體端節酵素活性(telomerase activity)
的偵測…………………………………………20
肆、結果…………………………………………………24
一、植物組織培養…………………………………24
二、端節酵素活性…………………………………28
伍、討論…………………………………………………40
陸、參考文獻……………………………………………44

陸、參考文獻
王自存。1995。切花的保鮮。園產品處理學實習講義。
江雅純、黃敏展。1997。菊花葉片癒傷組織之培養。興大園藝。 22:73~85。
林郁涵。2001。硫代硫酸銀及硫酸鋁對延長玫瑰與洋桔梗切花保鮮及玫瑰切花利用硫代硫酸銀處理與花瓣端節酵素活性變化之研究。國立高雄師範大學生物科學研究所碩士論文。中華民國。
洪信慧。1985。菸草原生質體培養之研究。國立台灣大學農藝學研究所碩士論文。中華民國。
洪嬡婷。1999。植物開花期間細胞內端節酵素之表現。國立高雄師範大學生物科學研究所碩士論文。中華民國。
倪月荷、汪先覺。1994。實用生活叢書。園藝系列14菊花。
黃勝忠、許謙信。2002。菊花品種的改良-從雜交育種到生物技術之應用。科學發展。351:18~23。
廖麗貞。1986。菸草屬植物種間原生質體分離、融合與種間體細胞雜種培育之研究。國立台灣大學農藝學研究所碩士論文。中華民國。
歐貞君。2000。菊花原生質體培養與基因轉質系統之建立。國立高雄師範大學生物科學研究所碩士論文。中華民國。
Bhattacharya, P., Dey, S., Das, N., and Bhattacharyya, B. C. 1990. Rapid mass propagation of Chrysanthemum morifolium by callus derived from stem and leaf explants. Plant Cell Reports. 9:439~442.
Bryan, T. M., Sperger, J. M., Chapman, K. B., and Cech, T. R. 1998. Telomerase reverse transcriptase genes identified in Tetrahymena thermophila and Oxytricha trifallax. Proc. Natl. Acad. Sci. USA. 95:8479~8484.
Broun, P., Ganal, M. W., and Tanksley, S. D. 1992. Telomeric arrays display high levels of heritable polymorphism among closely lelated plant varieties. Proc. Natl. Acad. Sci. USA. 89:1354~1357.
Burr, B., Burr, F. A., Matz, E. C., and Romero-Severson, J. 1992. Pinning down loose ends: mapping telomeres and factors affecting their length. Plant Cell. 4:953~960.
Fajkus, J., Fulneckova, J., Hulanova, M., Berkova, K., Riha, K., and Matyasek, R. 1998. Plant cells express telomerase activity upon transfer to callus culture, without extensively changing telomere lengths. Mol Gen Gent. 260:470~474.
Fajkus, J., Kovarik, A., and Kralovics, R. 1996. Telomerase activity in plant cells. FEBS Letters. 391:307~309.
Fitzgerald, M. S., Riha, K., Gao, F., Ren, S., McKnight, T. D., and Shippen, D. E. 1999. Disruption of the telomerase catalytic subunit gene from Arabidopsis inactivates telomerase and leads to a slow loss of telomeric DNA. Proc. Natl. Acad. Sci. USA. 96(26):14813~14818.
Fitzgerald, M. S., McKnight, T. D., and Shippen, D. E. 1996. Characterization developmental patterns of telomerase expression in plants. Proc. Natl. Acad. Sci. USA. 93:14422~14427.
Fuchs, J., Branders, A., and Schubert, I. 1995. Telomere sequence localization and karyotype evolution in higher plants. Plant Syst. Evol. 196:227~241.
Greider, C. W., and Blackburn, E. H. 1996. Telomoeres, Telomoerase and Cancer. Scientific American .274:80~85.
Heller, K., Kilian, A., and Piatyszek, M. A. 1996. Teleomerase activity in plant extracts. Mol Gen Genet.252:342~345.
Jong, J. de., Rademaker, W., and Wordragen, M. F. 1993. Restoring adventitious shoot formation on chrysanthemum leaf explants following cocultivation with Agrobacterium tumefaciens. Plant Cell. 32:263~270.
Killan, A., Heller, K., and Kleinhofs, A. 1998. Development patterns of telomerase activity in barley and maize. Plant Mol. Biol. 37:621~628.
Killan, A., Stiff, C., and Kleinhofs, A. 1995. Barley telomeres shorten during differentiation but grow in callus culture. Proc. Natl. Acad. Sci. USA. 92:9555~9559.
Lu, C. Y., Nugent, G., and Wardley, T. 1990. Efficient, direct plant regeneration from stem segments of chrysanthemum. Plant Cell Reports. 8:733~736.
Marcand, S., Brevet, V., Mann, C., and Gilson, E. 2000. Cell cycle restriction of telomere elongation. Current Biology. 10:487~490.
McKnight, T. D., Fitzgerald, M. S., and Shippen, D. E. 1997. Plant telomeres and telomerases. A review. Biochemistry (Moscow). 62:1224~1231.
McClintock, B. 1941. The stability of broken ends of chromosome in Zeamays. Genetics. 41:234~282.
Nugent, C. I., and Lundblad, V. 1998.The telomerase reverse transcriptase: components and regulation. Genes & Development. 12:1073~1085.
Oguchi, K., Liu, H., Tamura, K., and Takahashi, H. 1999. Molecular cloning characteristion of AtTERT, a telomerase reverse transcriptase homolog in Arabidopsis thaliana. FEBS Letters. 457:465~469.
Riha, K., Fajus, J., Sirokey, J., and Vyskot, B. 1998. Developmental control of telomere lengths and telomerase activity in plants. Plant Cell. 10:1691~1698.
Sauvadet, Marie-A., Brochard, P., and Boccon-Gibod, J. 1990. A protoplast-to-plant system in chrysanthemum : differential responses among several commercial clons. Plant Cell Rep. 8:692~695.
Schum, A., and Preil, W. 1981. Regeneration von Kallus aus Mesophyll - Protoplasten von Chrysanthemum morifolium Ramat.
Gartenbauwissenschaft. 469:91~93.
Segers, G., Gadisseur, I., Bergounioux, C., Engler, J. de A., Jacqmard, A., Montagu, M. V., and Inze, D. 1996. The Arabidopsis cyclin- dependent Kinase gene cdc2bAt is preferentially expressed during S and G2 phases of the cell cycle. The Plant Journal. 10(4):601~612.
Tamura, K., Liu, H., and Takahashi, H. 1999. Auxin induction of cell cycle regulated activity of tobacco telomerase. J. Biol. Chem. 274: 20997~21002.
Trehin, C., Planchais, S., Glab, N., Pernnes, C., Tregear, J., and Bergounioux, C. 1998. Cell cycle regulation by plant growth regulators: involvement of auxin and cytokinin in the re-entry of Petunia protoplasts into the cell cycle. Planta. 206:215~224.
Trigiano, R. N. 1996. Isolation of protoplasts from leaves of chrysanthemum and orchardgrass. Plant Tissue Culture Concepts and Laboratory Exercises. PP.227~233.
Zentgraf, U. 1995. Telomere-binding proteins of Arabidopsis thaliana. Plant Mol. Biol. 27:467~475.

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