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研究生:林鴻仁
研究生(外文):Hung-Jen Lin
論文名稱:文心蘭花粉稔實之研究
論文名稱(外文):Studies on Pollen Fertility of Oncidium spp.
指導教授:廖麗貞廖麗貞引用關係陳燿煌
指導教授(外文):Li-Jen LiaoYao-Huang Chen
學位類別:碩士
校院名稱:國立高雄師範大學
系所名稱:生物科學研究所
學門:生命科學學門
學類:生物學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:中文
論文頁數:78
中文關鍵詞:文心蘭
外文關鍵詞:Oncidium
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中文摘要
文心蘭雜交成功率低,限制新品種的開發。花粉不稔實為雜交失敗主因之一。花粉之稔實性取決於花粉本身是否成熟有活力,自交或異交與柱頭的親合性,花粉管是否能在中空花柱引導組織中生長,及能否順利進入子房與胚珠中之卵受精。觀察花粉之染色、體外及體內萌發過程,有助於建立文心蘭花粉發育與授粉行為之基本資料,以提供日後研究參考。本實驗結果顯示:文心蘭開花時,四分體細胞中無澱粉累積,但具有粒腺體活性與乙酯酶的活力。體外發芽實驗中,添加3%蔗糖的培養液可達最佳萌發效果,以此濃度作為標準,可作為初步篩選稔實花粉參考,而得出不同種文心蘭花粉,具有不同的稔實性差異。柱頭發芽實驗中,Angel ×Golden Shower "Liu Li "或 Ramsey ×Golden Shower "Liu Li "之組合,其親合性花粉塊授粉於柱頭上,2天即見發芽,5天可見伸入柱頭生長,8-11天可見伸入子房上部,14天時花粉管生長可抵子房末端,23-25天可見花粉管穿入胎座生長,估計於28-35天期間完成受精,42天時可見發育中的胚囊。

Abstract
The low successful ratio of intra- or inter-cross in Oncidium defines new species product. Pollen viability is one of the main reasons. Successful inter-cross is closely related with the compatibility of the pollen grains on stigma, the capability of pollen grains growth in the style tracting tissues, and the successful entrance into the ovary for pollination. The pollen was observed by stain methods and in vitro or in vivo tests germination in order to found Oncidium pollination basic data for hybrid breeding.
The results of this research are summarized as follow: it is clear that Oncidium pollen grains develop stop at tetrad stage and can not reach the true mature, so there is no starch accumulation. But the viability of the mitochondria and esterase had expression. When 3% sucrose were added to the culture medium, it was found that Oncidium pollen grains exhibited high viability. When using this culture medium to investigate different species pollen grains of the Oncidium, showed the different species pollen grains displayed different germination potential and viability.
Angel ×Golden Shower "Liu Li " or Ramsey ×Golden Shower "Liu Li " belongs to the compatible inter-cross. After pollination, Golden Shower "Liu Li " pollen grains germinated about 2 days on stigma surface, grew into stigma about 5 days, grew into the upper ovary 8-11 days, and grew into the placenta 23-25 days. Approximately 28-35 days for inseminating and unmature seeds were visible about 42 days after pollination .

目錄
中文摘要.......................... i
英文摘要.......................... ii
目錄
壹、前言.......................... 1
貳、前人研究........................ 2
一、花粉的發育..................... 2
二、花粉萌芽活力之探討................. 5
三、柱頭上花粉的萌發及誘導研究............. 7
四、柱頭-花粉等之自交不親合性.............. 8
五、花粉管與花柱引導組織( stylar transmitting tract )..... 14
六、花粉管的生長....................14
七、胚囊引導花粉之受精作用...............19
參、材料及方法.......................22
肆、結果.......................... 27
一、以染色技術偵測花粉稔實能力試驗........... 27
〈一〉碘液染色................... 27
〈二〉MAO染色...................27
〈三〉螢光染色................... 28
二、花粉發芽試驗.................... 28
〈一〉花粉體外發芽................. 28
〈二〉花粉體內發芽與花粉管生長........... 30
伍、討論.......................... 68
(一)以染色技術偵測花粉稔實能力探討.......... 68
(二)花粉發芽試驗之探討................ 69
陸、參考文獻........................ 74

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