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研究生:楊苑欣
研究生(外文):Yang Yuann Shin
論文名稱:火鶴花試管苗葉片培養植株再生之研究
論文名稱(外文):Plant Regeration from in vitro Lamina Culture of Anthurium
指導教授:陳福旗陳福旗引用關係
指導教授(外文):Fure chyi chen
學位類別:碩士
校院名稱:國立屏東科技大學
系所名稱:熱帶農業研究所
學門:農業科學學門
學類:一般農業學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:中文
論文頁數:47
中文關鍵詞:火鶴花
外文關鍵詞:Anthurium
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本試驗以火鶴花試管苗葉片為材料,培養於添加不同細胞分裂素之修正MS配方,結果以添加0.1~0.5 mg/l TDZ 癒合組織誘導率97.5~100%較高。在含有0.5 mg/l TDZ之培養基所產生之癒合組織最大。以0.1、0.2 mg/l BA、或0.5 mg/l Kinetin等三種處理之培植體產生的不定芽最多,約為70%。添加0.2 mg/l BA之培養基誘導產生的不定根數最多。0.5mg/l TDZ 與0.5 mg/l 2, 4-D組合促進不定芽、不定根形成,TDZ與Picloram組合,誘導產生體胚狀構造。生長素單獨使用不利於火鶴花T2品種葉片再生,半量MS的鹽類濃度有助於癒合組織的形成。不同品種間不定芽的再生能力有差異。
Abstract
Lamina of in vitro grown anthurium plantlets were used for the study of callus induction and shoot regeneration. Modified MS medium containing three cytokinins was used as regeneration medium. The highest callus formation was observed on basal medium supplemented with 0.1~0.5 mg/l TDZ, which also induced larger calli. On the media containing 0.1~0.2 mg/l BA, or 0.5mg/l kinetin, 70% of the explants regenerated adventitious shoots. Root formation was observed on medium containing 0.2 mg/lBA. Somatic embryos were induced on medium containing 0.5mg/l TDZ and 0.2-0.5 mg/l picloram. Auxin alone was not effective for callus induction and shoot regeneration from T2 lamina. Reducing salt strength to one half promoted callus induction. Regeneration ability is statistically different among genotypes.
目 錄
中文摘要 Ι
英文摘要 ΙΙ
誌謝 ΙΙΙ
目錄 ΙV
圖次索引 VΙ
表次索引 VΙΙ
壹、前言 1
貳、前人研究 2
一、火鶴花的分類地位及特徵 2
二、組織培養 3
(一)培植體的來源 4
(二)基因型的差異 4
(三)消毒方式 5
(四)組織的成熟度 5
(五)培養基的組成 5
1、培養基的形態 5
2、荷爾蒙誘導培植體再生 6
3、醣類對癒合組織生成及體胚發生之影響 7
4、荷爾蒙促進叢生芽增殖之影響 7
5、培養基的含量 8
6、氮含量對再生之影響 8
(六)物理因子 8
三、火鶴花的染色體數 9
參、材料與方法 11
一、植物材料 11
二、植物生長調節劑對火鶴花葉片再生之影響 11
三、統計分析 13
四、根尖染色體數的檢查 13
五、組織切片與觀察 14
(一)植物材料 14
(二)組織切片方法 14
肆、結果 17
一、植物生長調節劑對火鶴花試管苗葉片再生之影響 17
(一)不同細胞分裂素對T2品種再生之影響 17
(二)細胞分裂素及生長素對T2及T18品種再生之影響
23
(三)生長素單獨處理對火鶴花T2品種葉片再生之影響
23
(四)鹽類濃度對火鶴花葉片再生之影響 24
(五)品種間不定芽發生的比較 24
二、再生植株的根尖染色體數 25
伍、討論 40
陸、參考文獻 44
陸、參考文獻
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