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研究生:閻大方
研究生(外文):Da-Fun Yan
論文名稱:白蝦高密度循環水養殖及其脫殼週期與大小對血淋巴因子變動之研究
論文名稱(外文):Studies on the super-intensive recirculation system for culturing the whiteleg shrimp, Litopenaeus vannamei Boone, and the effects of its molting cycle and size on hemolymph variables
指導教授:鄭文騰鄭文騰引用關係葉信平葉信平引用關係
指導教授(外文):Winton ChengShinn-Pyng Yeh
學位類別:碩士
校院名稱:國立屏東科技大學
系所名稱:熱帶農業研究所
學門:農業科學學門
學類:一般農業學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:中文
論文頁數:77
中文關鍵詞:白蝦攜氧血藍素蛋白質滲透壓離子性別大小脫殼週期高密度養殖
外文關鍵詞:Litopenaeus vannameiOxyhemocyaninProteinOsmolalitiyIonsSexSizeMolt stageIntensive culture
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本文第一部份在係利用循環水以小面積水泥池打氣及池頂用蘭花網遮蓋。由於放養期間歷經冬季自11月至隔年2月,故池水溫度皆低於20℃。白蝦當在24℃低溫下,會停止攝餌及成長。故養殖期間延長,養殖天數增加,在放養後第190天,蝦重約達10公克。在高密度養殖白蝦蝦苗396.83尾/m2,活存率高達70%。單位產量達每噸2.92kg。
第二部份主為研究白蝦 (Litopenaeus vannamei. Boone, 1931)不同大小、性別及脫殼週期之血淋巴攜氧血藍素、蛋白質、滲透壓及離子濃度之相關性。結果發現不同性別之白蝦(4.69~32.57g),其血淋巴之相關數值間並無顯著性差異。在脫殼週期上發現,D0/D1(脫殼前期)之血淋蛋白質及攜氧血藍素濃度最高 (132.56±2.79及1.70±004 mmol/l),而在脫殼A期 (脫殼後期)濃度最低 (87.54±2.28 mg/ml及1.08±0.05 mmol/l)。血淋巴滲透壓及氯離子濃度在D0/D1期最高(798±6.3 mOsm/kg及276.8±2.2 mmol/l),而在脫殼A期則濃度最低 (760.7±2.3 mOsm/kg 及241.5±3.8 mmol/l)。血淋巴蛋白質、攜氧血藍素、滲透壓、氯離子、鈉離子、鉀離子、鎂離子及鈣離子濃度會隨體型增加而增加,可能與白蝦在蝦池成熟有關。蛋白質、滲透壓、氯離子、鈉離子及鉀離子值在脫殼後期時最低,但在脫殼間期及早期之脫殼前期最高,這可能是與脫殼時之吸水有關。
The first part of this study is to use recirculation technology to reduce water requirements less discharge from the culture system, minimize environmental impacts, and offer a high degree of biosecurity. Culture tanks A, B, C were stocked at 396.8 pcs/m2 and harvested after 189 days, with an average weight 4 ponds 10.4, 10.3, 9.5g. Ponds D and E were stocked at the density as above and harvested after 189, 264 days. With average weight of 10.2 and 15.1g, respectively. Unit production was 1.98~2.12kg/m2 of these trials cultured Litopenaeus vannamei shrimp in tanks A, B, C, D. The days of stocked are 190. When period in rearing time pass seasons cool. Its temperature low to 20℃ from mid-october to next year march . Water temperature less than 24℃ will or halt feeding in whiteleg shrimp(Litopenaeus vannamei). But them are good survival at up to 70%.
The second part of this study is the whiteleg shrimp Litopenaeus vannamei (Boone, 1931) was examined for hemolymph oxyhemocyanin, protein, osmotic and ion concentration in relation to sex, size and molt stage. No significant differences in hemolymph variables were observed between male and female shrimps with body weight in the range of 4.7-32.6 g. Among the molt cycles, hemolymph protein and oxyhemocyanin levels were highest (132.56±2.79 mg/ml and 1.70±0.04 mmol/l) at stage D0/D1, and lowest (87.54±2.28 mg/ml and 1.08±0.05 mmol/l) stage A, respectively. In addition, Hemolymph osmolarity and chride concentration were the highest (798±6.3 mOsm/kg and 276.8±2.2 mmol/l) at stage D0/D1, and lowest (760.7±2.3 mOsm/kg and 241.5±3.8 mmol/l at stage A. The increase of hemolymph protein, oxyhemocyanin, osmolality, Na+, Cl-, K+ Mg2+, and Ca2+ levels with the increase shrimp size indicating that the shrimp mature in the culture ponds. The fact that the hemolymph protein, osmolality, Na+, K+ and Cl-, levels were lower during the postmolt and higher during intermolt and early premolt are considered to be associated with water uptake at molt stage.
中文摘要....................... Ⅰ
英文摘要....................... Ⅲ
第一章 緒言..................... 1
第二章 文獻整理................... 3
第三章 材料與方法.................. 11
第四章 結果..................... 17
第五章 討論..................... 24
第六章 結論..................... 32
第七章 參考文獻.......................... 34
圖.......................... 40
表.......................... 57
附錄......................... 59
作者簡介....................... 77
中華民國台灣地區漁業統計年報,2000。行政院農業委員會漁業署。
吳昔家(1995)淡水也可以養草蝦。養魚世界。19:22-27。
王世經、張銘智(2000)。超集約室外循環水系統養殖日本鰻。生物資源生物技術,vol. 2(3):51-56。
林明男、曾寶順(1997)。室內養蝦的基礎研究-沙蝦以每平方公尺500尾的高密度養殖。養魚世界,6:14-21。
余俊億 (2000)。太平洋白蝦感染桃拉症病毒後血漿之分析,國立台灣大學動物研究所碩士論文。
邱建樺 (2001)。白蝦在不同鹽度環境的成長與生理反應之研究,國立台灣大學動物研究碩士論文。
陳弘成(1995)。水質管理。農委會漁業特刊,51:1-5。
陳弘成(1994)。蝦類養殖病害與管理。養魚世界。2:23-29。
陳弘成(1992)。成功的草蝦養殖法-超集約式高產量的蝦類養殖法。農委會特刊,13:25-26。
陳獻、梁榮元、賴國興、張明輝、林明男、丁雲源(2000)。室內立體式自動化養蝦系統之研發。台南水產試驗所研究報告,3:65-78。
廖一久(1995)。台灣養殖漁業自動化研究發展的回顧與展望。養殖漁業生產自動化成果發表暨研討會論文摘要,1-2。
盧民益、黃美瑩、張錦宜、張振元、劉文御、徐崇仁(2000)。白蝦(Litopenaeus vannamei)室內高密度養殖初步檢驗。養魚世界,12:69-77。
Balazs, G. H., Olbrich, S. E., Tumbleson, M. E., 1974. Serum constituents of the Malaysian prawn (Macrobrachium rosenbergii) and pink shrimp (Penaeus marginatus). Aquaculture 3:147-157.
Bendschneider, K., and Robinson, R. J., 1952. A new spectrometric method for the determination of nitrite in the sea water. J. Mar. Res. 11:87-96.
Bradford, M. M., 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein using the principle of protein-dye binding. Analyt. Biochem. 72:248-254.
Bursey, C. R., Lane, C. E., 1971. Ionic and protein concentration changes during the molt cycle of Penaeus duorarum. Com. Biochem. Physiol. 40A:155-162.
Busselen, P., 1970. Effects of moulting cycles and nutritional conditions on haemolymph proteins in Carcinus maenas. Comp. Biochem. Physiol. 37-83.
Castille, F. L. Jr., Lawrence, A. I., 1981. The effect of salinity on the osmotic, sodium, and chloride concentrations in the haemolymph of euryhaline shrimp of the genus Penaeus. Comp. Biochem. Physiol. 68 A:75-80.
Chen, J. C., Chen, C. T., 1996. Changes of osmotic and electrolyte concentrations in the haemolymph of Penaeus japonicus exposed to ambient ammonia. Comp. Biochem. Physiol. 114C:35-38.
Chen, J. C., Cheng, S. Y., 1993. Studies on haemocyanin and haemolymph protein levels of Penaeus japonicus based on sex, size and moulting cycle. Comp. Biochem. Physiol. 106 B:293-296.
Chen, J. C., Chia, P. G., 1997. Oxyhaemocyanin, protein, osmolality and electrolyte levels in the hemolymph of Scylla serrata in relation to size and molt cycle. J. Exp. Mar. Biol. Ecol. 217:93-105.
Cheng, W., Liu, C. H., Cheng, C. H., Chen, J. C., 2001. Hemolymph oxyhemocyanin, protein, osmolality and electrolyte levels of Macrobrachium rosenbergii in relation to size and molt stage. Aquaculture 198:387-400.
Clark, J. V. 1986. Inhibition of moulting in Penaeus semisulcatus (De Haan) by long-term hypoxic. Aquaculture 52:253-254.
DeLeersnyder, M., 1967. Le milieu interieur d’Eriocheir sinensis Milne-Edwards et ses variations. I. Etude dans le milieu naturel. Cahiers de Biologie Marine 8:195-218.
Djangmah, J. S., 1970. The effects of feeding and starvation on coper in the blood and hepatopancreas, and on blood proteins of Crangon vulgaris (Fabricius). Comp. Biochem. Physiol. 32:709-731.
Djangmah, J. S., Grove, D. J., 1970. Blood and hepatopancreas copper in Crangon vulgaris (Fabricius). Comp. Biochem. Physiol. 32: 733-745.
Duncan, D. B., 1955. Multiple-range and multiple F test. Biometrics. 11:1-42.
Farfante, I. P., Kensley, B., 1997. Penaeid and sergestoid shrimps and prawns of the world: Keys and diagnoses. Memoires du Museum National D’Histoire Naturelle, Paris.
Ferraris, R. P., Parado-Estepa, F. D., Ladja, J. M., 1986. Effect of salinity on the osmotic, chloride, total protein and calcium concentrations in the hemolymph of the p rawn Penaeus monodon (Fabricius). Comp. Biochem. Physiol. 83A:701-708.
Fieber, L. A., Lutz, P. L., 1982. Calcium requirements for molting in Macrobrachium rosenbergii. J. World Maricult. Soc. 13:21-27.
Gills, R., 1977. Effects of osmotic stresses on the protein concentration and pattern of Eriocheir sinensis blood. Comp. Biochem. Physiol. 56A:109-114.
Greenaway, P., 1985. Calcium balance and moulting in the Crustacean. Bio. Rev. 60:425-454.
Hagerman, L., 1983. Haemocyanin concentration of juvenile lobster (Homarus gammarus) in relation to moulting cycle and feeding conditions. Mar. Biol. 77:11-17.
Hagerman, L., 1986. Haemocyanin concentration in the shrimp Crangon crangon (L.) after exposure to moderate hypoxia. Comp. Biochem. Physiol. 85A:721-724.
Knowlton, R. E., Williams, A. B. 1970. The life history of Palaemonetes vulgaris (Say) and P. pugio holthuis in coastal North Carolina. J. Elisha Mitchell Sci. Soc. 86:158.
Lightner, D. V., Hedrick, R. P., Fryer, J. L., Chen, S. N., Liao, I. C. And Kou, G. H., 1987. A survey of cultured penaeid shrimp in Taiwan for viral and other important disease. Fish. Pathol. 22:127-140.
Lo, C. F., Lou, G. H., 1998. Virus-associated white spot syndrome of shrimp in Taiwan: A review. Fish Pathol. 33:365-371.
Menz, A., Blake, B. F., 1980. Experiments on the growth of Penaeus vannamei Boone. J. Exp. Mar. Biol. Ecol. 48:99-111.
Mercaldo-Allen, R., 1991. Changes in the blood chemistry of the American lobster, Homarus americanus, H. Milne Edwards, 1837, over the molt cycle. J. Shellfish Res. 10:147-156.
Nicol, J. A., 1967. The biology of marine animals. Pitman London. 699.
Nickerson, K. W., Van Holde, K. E., 1971. A comparison of molluscan and arthropod hemocyanin. Ⅰ. Circular dichroism and absorption spectra. Comp. Biochem. Physiol. 39B:855-872.
Ong, K. S., 196. The early developmental stages of Scylla scrrata Forskal (Crustacea Portunidae), reared in the laborary. Indo-pac. Fish. Coune. Proc. 11th Sess., Sect., 2:135-146.
Parado-Estepa, F. D., Ladja, J. M., de Jesus, E. G., Ferraris, R. P., 1989. Effect of salinity on hemolymph calcium concentration during the molt cycle of the prawn Penaeus monodon. Mar. Biol. 102:189-193.
Perez-Farfante, I., Kensley, B. F., 1997. Penaeoid and sergestoid shrimp and prawns of the world: keys and diagnoses for the families and genera. Memoires Du Museum National D, Histoire naturelle Tome 175:1-233.
Prosser, C., 1973. Water: Osmotic balance, hormonal regulation; Inorganic ions. In ”Comparative Animal Physiology” Sauners. Philadelphia. 1-100.
Roberts, M. H., 1971. Larval development of Pagirrus longicarpus Say reared in the laboratory. Ⅱ. Effects of reduced salinity on larval development. Biol. Bull. Woods Hole. 140:104-116.
Robertson, J. D., 1960 Ionic regulation in the crab Carcinus maenas (L.) in relation to the molting cycle. Comp. Biochem. Physiol. 1: 183-212.
Robertson, L., Bray, W., Leung-Trujillo, J., Lawrence, A., 1987. Practical molt staging of Penaeus setiferus and Penaeus stylirostris. J. World Aquacult. Soc. 18:180-185.
SAS, 1988. SAS/STAT user’s guide. 6.03 edition, SAS Institution Inc., Cary, NC.
Snieszko, S. F., 1973. Diseases of fishes and their control in the U. S. In: The Two Lakes Fifth Fishery Management Tralining Course Report. Jansen, London. 55-66.
Towle, D. W., Mangum, C. P., 1985. Ionic regulation and transport ATPase activities during the molt cycle in the blue crab Callinectes sapidus. J. Comp. Physiol. 5:216-223.
Truchot, J. P., 1978. Variations de la concentration sanguine d’hemocyanin fonctionelle au cours du cycle d’intermue chez le crabe Carcinus maenas (L.). Arch. Zool. Exp. Gen. 119:265-282.
Tu, C., Huang, T. H., Chuang, S. H., Hsu, J. P., Kuo, S. T., Li, N. J., Hsu, T. L., Li, C. M., Lin, S. Y., 1999. Taura syndrome in Pacific white shrimp Penaeus vannamei cultured in Taiwan. Dis. Aquatic. Org. 38:159-161.
Vazquez-Boucard, C. G., Moureau, C. E.,m Ceccaldi, H., 1985. Etude preliminaire des variations circadiennes des proteins de I’hemolymphe de Penaeus japonicus Bate.J. Exp. Biol. Ecol. 85: 123-133.
Vernberg, F. J., Vermberg, W. B., 1970. “The Animal in the Environment. “Holt, Rinehart and Winston, New York. 1-462.
Vijayan, K. K., Diwan, A. D., 1996. Fluctuations in Ca, Mg and P levels in the hemolymph, muscle, midgut gland and exoskeleton during the moult cycle of the indian white prawn, Penaeus indicus (Decapoda: Penaeidae). Comp. Biochem. Physiol. 114A:91-97.
Wright, D. A., 1979. Calcium regulation in intermolt Gammarus pulex. J. Exp. Biol. 83:131-144.
Wyban, J., Walsh, W. A., Godin, D. M., 1995. Temperature effects on growth, feeding rate and feed conversion of the Pacific white shrimp (Penaeus vannamei). Aquaculture. 138:267-279
Van Wyk, Peter M., Pierce, F. 2000. Culture of Penaeus vannamei in single-phase and three-phase recirculating aquaculture systems. The Advocate. June. 41-43
Yu, C.I., Song, Y. L., 2000. Outlooks of Taura syndrome in Pacific white shrimp Penaeus vannamei culture in Taiwan. Fish Pathol. 35:21-24.
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