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研究生(外文):Chung-Ren Lin
論文名稱(外文):Exploiting the Potential Therapy for Neuropathic Pain Through Cellular and Molecular Approaches
指導教授:陳 錦 翠
指導教授(外文):Jiin-Tsuey Cheng
外文關鍵詞:gene therapyneuropathic paincell therapyelectroporationnon-viral gene transferintrathecal
  • 被引用被引用:0
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1.0 Background
1.1 Neuropathic Pain
1.2 Cell Therapy for Neuropathic Pain
1.3 Gene therapy for Neuropathic Pain
2.0 General Research Objectives
2.1 Specific Aims
3.0 Experimental Design
3.1 Intrathecal Spinal Progenitor Cell Therapy
3.2 Intrathecal Electroporation Therapy
4.0 Materials and Methods
4.1 Animal Preparation
4.2 Animal Model of Neuropathic Pain
4.3 Intrathecal Catheterization
4.4 Electrode-Catheter Construction
4.5 Mini Osmotic Pump Implantation
4.6 Behavioral Assessment
4.7 CSF Microdialysis
4.8 Preparation of SPCs
4.9 Flow Cytometry Analysis
4.10 Amino Acid Analysis
4.11 RT-PCR for Ribonucleic Acid (RNA) Analysis
4.12 Western Blotting
4.13 Human Pro-opiomelanocortin (POMC) cDNA Expression Plasmid Construction
4.14 Perfusion-Fixation in vivo
4.15 Immunohistochemical Staining
4.16 Immunoreactive Cells Counting
4.17 c-Fos Antibody Electroporation
4.18 Luciferase Assay for AP-1 Promoter Activity
4.19 Data Analysis
5.0 Results
5.1 Intrathecal Spinal Progenitor Cell Therapy
5.2 Intrathecal Electroporation Therapy
6.0 Discussion
7.0 References
8.0 Table
9.0 Figures and Legends
Publications and Presetations Related to this Thesis
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