臺灣博碩士論文加值系統

EB病毒的感染在懷孕的婦女身上是很常見的，母親的免疫系統被壓抑，而各種抗體越過胎盤保護胎兒對抗初級感染達數個月。LMP1在EB病毒的潛伏性感染扮演一個很重要的角色，包括LMP1會造成B細胞表面附著分子的增加，導致細胞聚集，使得細胞間分泌的自主性生長因子濃度增高，可造成細胞生長速率變快，刺激細胞轉型或增生，其次是改變B細胞表面標記抑制細胞自殺，抑制表皮細胞的分化，藉由細胞訊號傳遞路徑來調節對細胞生長的影響。我們藉由高雄市小港區，45例人類胎盤EB病毒LMP1的檢驗，發現共8例(18%)呈現陽性反應，其餘37例(82%) 皆為陰性反應，且細胞膜及細胞質具有深紅色陽性染色結果都在靠近母體那一側的細胞，而在靠近胎兒那一側的細胞染色則呈現陰性反應。EB病毒無法經由胎盤進入胎兒內說明胎盤對胎兒提供了適當的保護。

In normal pregnant women, reactivation of Epstein-Barr virus (EBV) frequently occurs. Cellular immune response is apparently supperessed, but the antibodies cross the placenta and protect the infant against primary infection for many months. The Epstein—Barr virus (EBV) encoded latent membrane protein 1 (LMP1) which plays a crucial role in the long-term persistence of this virus within the cells of the immune system. Not only is this protein critical for the transformation of resting B cells by EBV, it also displays pleiotropic effects on various cellular proteins expressed in the host cell. These include upregulation of express- ion of B cell activation antigens, adhesion molecules, and various components of the antigen processing pathway. Here we test 45 human placenta samples for LMP1 of Epstein—Barr virus (EBV) by RT-PCR and immunohistochemistry. Eight of forty five (18%) are positive , and thirty seven of forty five (82%) are negative for LMP expression. It is noticed that all LMP protein expression were shown on the maternal side but not on the fetal side. The placenta may serve as a protection barrier for fetus against Epstein-Barr virus infection.

目錄壹、緒論---------------------------------------------------------4
一、前言-------------------------------------------------------4
二、胎盤(placenta) ---------------------------------------------5
三、EBV---------------------------------------------------------6
四、LMP1（潛伏性膜蛋白1）---------------------------------------7
五、最近研究所知的LMP1功能-------------------------------------8
貳、研究目的-----------------------------------------------------12
參、材料與方法---------------------------------------------------13
一、培養血癌細胞RPMI--------------------------------------------13
二、胎盤檢體---------------------------------------------------13
三、Total RNA之萃取--------------------------------------------14
四、反轉錄酵素反應----------------------------------------------15
五、LMP1 mRNA的PCR增殖反應------------------------------------15
六、定序與序列比對----------------------------------------------16
七、免疫組織染色(immunohistochemistry; IHC) ---------------------17
肆、實驗結果-----------------------------------------------------19
伍、討論----------------------------------------------------------22
陸、結論----------------------------------------------------------23
柒、參考文獻------------------------------------------------------39
表:
表一-------------------------------------------------------------24
表二-------------------------------------------------------------25
圖:
圖一-------------------------------------------------------------27
圖二-------------------------------------------------------------28
圖三-------------------------------------------------------------29
圖四-------------------------------------------------------------30
圖五-------------------------------------------------------------31
圖六-------------------------------------------------------------32
圖七-------------------------------------------------------------33
圖八-------------------------------------------------------------34
圖九-------------------------------------------------------------35
圖十-------------------------------------------------------------36
圖十一-----------------------------------------------------------37

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