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研究生:徐秀敏
研究生(外文):Shiou-Min Shiu
論文名稱:以粒線體核酸分析探討台灣長鬃山羊之親緣關係與生物地理
論文名稱(外文):Phylogeny and Biogeography of the Capricornis swinhoei Based on Mitochondrial DNA Sequences
指導教授:張學文張學文引用關係
指導教授(外文):Hsueh-Wen Chang
學位類別:碩士
校院名稱:國立中山大學
系所名稱:生物科學系研究所
學門:生命科學學門
學類:生物學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:中文
論文頁數:99
中文關鍵詞:粒線體核酸台灣長鬃山羊親緣關係生物地理
外文關鍵詞:Capricornis swinhoeiMitochondrial DNAPhylogenyBiogeography
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  • 被引用被引用:6
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摘 要
台灣長鬃山羊(Capricornis swinhoei)為台灣特有的保育類野生動物,本研究的目的乃藉由分子生物學以及形態學的特徵,探討台灣長鬃山羊族群內的遺傳歧異度、觀測族群之架構、分化情形以及生物地理親緣關係。採樣包括本島各縣市之山地及其主要山脈的野生長鬃山羊族群樣本,使用粒線體DNA的控制區序列作為比較的依據,因其具有不受有性生殖再重組的影響,以及演化速度快的優點,可解答種內地理親緣關係的問題,以此片段利用GCG巨分子分析軟體排序及分析,並以MEGA分析軟體計算其遺傳距離進行鄰接法(neighbor-join) 、最大簡約法(maximum parsimony)支序分析建構其親緣關係樹,研究的結果將台灣長鬃山羊分為南北兩群,而頭骨形態測量分析亦支持這樣的結果,配合地理分佈、生態習性與巨觀的地質資料,建立起台灣長鬃山羊的族群以及親緣關係的資訊,將可提供保育工作之參考。
Abstracts
Capricornis swinhoei is one of the indangered wild animals in Taiwan. The objective of this study is using molecular biology and morphology methods to analyze the genetic diversity, observed population structure, differentiation and biogeographic relationships among the population of Capricornis swinhoei. Samples used in this study were collected from the Central Mountains located in Taiwan. DNA sequences (1099 bp) from the mitochondrial DNA (mtDNA) control region, D-loop, were used to test the phylogeographic relationships among the Capricornis swinhoei. A phylogenetic tree was constructed on the basis of GCG-Seq Web and MEGA softwares. In addition, distance analyses, neighbor-join, and maximum parsimony to resolve these phylogenetic relationships were performed. The C. sumatraensis and C. crispus sequence were also determined and used as the outgroups. Our findings clearly demonstrate that Capricornis swinhoei can be clustered into two groups, north and south groups, and consistent with the truth of geographic isolation. The data obtained from this study may facilitate the program of wildlife conservation in Taiwan.
目 次
中文摘要--------------------------------------------------------------------------- i
英文摘要-------------------------------------------------------------------------- ii
目 次------------------------------------------------------------------------- iii
表 次------------------------------------------------------------------------- v
圖 次------------------------------------------------------------------------- vi
第一章 前言------------------------------------------------------------------- 1
第二章 材料與方法---------------------------------------------------------- 5
第一節 樣本採集-------------------------------------------------------- 5
第二節 Total DNA萃取------------------------------------------------ 5
第三節 聚合酶鏈反應------------------------------------------------- 7
第四節 電泳膠分析---------------------------------------------------- 8
第五節 PCR產物純化------------------------------------------------ 8
第六節 DNA自動定序---------------------------------------------- 9
第七節 親緣關係分析軟體----------------------------------------- 9
第八節 頭骨型態特徵分析----------------------------------------- 11
第三章 結果--------------------------------------------------------------------19
第一節 粒線體DNA序列分析------------------------------------ 19
第二節 序列組成內容與鹽基置換-------------------------------- 19
第三節 序列相似度比較---------------------------------------------- 19
第四節 遺傳距離------------------------------------------------------- 20
第五節 親緣關係分析------------------------------------------------ 20
第六節 中性假說測試------------------------------------------------- 21
第七節族群內遺傳組成多型性之估算、族群結構與分化情形---------------------------------------------------- 21
第八節族群內遺傳組成多型性之估算--------------------------- 22
第九節族群間分化程度的估算------------------------------------ 22
第十節頭骨形態特徵分析------------------------------------------ 22
第四章 討 論--------------------------------------------------------------- 25
第五章 結 論--------------------------------------------------------------- 31
第六章 參考文獻----------------------------------------------------------- 32
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