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研究生:蔡政諭
研究生(外文):Jeng-Yu Tsai
論文名稱:草酸對於腎細胞甲一型微球蛋白生成的影響
論文名稱(外文):Expression of alpha-1-microglobulin mRNA change after oxalate challenge in renal epithelium cell
指導教授:趙大衛趙大衛引用關係
指導教授(外文):David, Chao
學位類別:碩士
校院名稱:國立中山大學
系所名稱:生物科學系研究所
學門:生命科學學門
學類:生物學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:中文
論文頁數:30
中文關鍵詞:尿路結石草酸蛋白質
外文關鍵詞:proteinoxalateurolithiasis
相關次數:
  • 被引用被引用:1
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甲一型微球蛋白(alpha-1-microglobulin)是一種與bikunin蛋白由同段基因(AMBP gene: □-1m-bikunin precursor gene)一起產生後,於細胞中分解而形成的蛋白質,大多在肝臟中製造,而後經由腎臟排出到尿液中。甲一型微球蛋白與bikunin蛋白皆被認為有抑制尿路結石生長的功能。本實驗利用草酸分子及草酸鈣經過的不等時間剌激近曲腎小管細胞(LLC-PK1),再以分子生物學技術萃取細胞中的核醣核酸,反轉錄成互補段的去氧核醣核酸,並利用甲一型球蛋白的引體進行聚合酶反應及即時聚合酶反應,以擴大產物總量,與原始互補段的去氧核醣核酸的總量比較,反推出原有細胞中AMBP信息核醣核酸的多寡。利用西方墨點法檢視蛋白質的生成,結果發現經過草酸及草酸鈣剌激後,信息核醣核酸及蛋白質均有增加的現象。
Alpha-1-microglobulin is co-synthesized with bikunin on the AMBP gene (□-1m-bikunin precursor gene). The major synthesis site of this protein is liver. It is excreted into urine by pass through glomerulus. Both alpha-1-microglobulin and bikunin are regarded as inhibitors of urolithiasis. This investigation is attempted to evaluate if mRNA in renal epithelium cell may be up-regulated by oxalate and oxalate crystal stimulation. LLC-PK1 cells were challenged with oxalate and calcium oxalate crystal. The messenger RNA was collected and reverse transcripted into cDNA. PCR and real time PCR methods using primer designed from rat alpha-1-microglobulin gene were performed to amplify cDNA in cellular samples. Western blot was used to monitor the amount of specific proteins produced in culture media and in cells. The result showed that cellular AMBP gene was up-regulated and alpha-1-microglobulin protein increased in culture media after treated with oxalate and calcium oxalate crystal.
目錄
壹、中文摘要-------------------------------------------------------2
貳、英文摘要 -----------------------------------------------------3
參、緒論 -----------------------------------------------------------5
肆、材料及方法 --------------------------------------------------8
伍、結果 -----------------------------------------------------------13
陸、討論 -----------------------------------------------------------16
柒、結論 -----------------------------------------------------------20
捌、參考文獻-----------------------------------------------------21
玖、圖 --------------------------------------------------------------25
Boyce WH.:Organic matrix of human urinary concretions. Am J Med 45(5):673-83, 1968. Herring LC. Observation on the analysis of ten thousand urinary calculi. J Urol 88:545-62, 1962 Atmani F, Lacour B, Drueke T, : Isolation and of a new glycoprotein from human urine inhibiting calcium oxalate crystallization. Urol Res. 21:61, 1993 Tejler L, Eriksson S, Crubb A, Astedt B: Production of protein HC by human fetal liver explants. Biochim Biophys Acta. 542:506-14, 1978 Akerstrom B, Logberg L, Berggard T, : □□-microglobulin-a yellow-brown lipocalin. Biochem Biophys Acta. 142:172-184, 2000 Dussol B, Geider S, Lilova A, : Analysis of soluble organic matrix of five morphologically different kidney stones. Urol Res. 23:45-51, 1995 Logdberg L, Akerstrom B: Immunosuppressive properties of alpha 1-microglobulin. Scand J Immunol. 13:383-90, 1981 Porstmann T, Schmechta H, Hentschel C, : Development of an immunoenzymometric assay for alpha 1-microglobulin and measurement of its serum concentration in normal and HIV-infected persons. J Chin Chem Chin Biochem. 28:669-675, 1990 Logdberg L, Akerstrom B, Shevash EM: Alpha 1-microglobulin is mitogenic for guinea pig lymphocytes. Scand J Immunol. 24:575-87, 1986 Wester L, Fast J, Labada T, .: Carbohydrate groups of alpha1-microglobulin are important for secretion and tissue localization but not for immunological properties. Glycobiology. 10:891-900, 2000 Bakiker-Mohamed H, Akerstrom B, Logdberg L: Mitogenic effect of alpha 1-microglobulin on mouse lymphocytes. Evidence of T- and B-cell cooperation, B-cell proliferation, and a low-affinity receptor on mononuclear cells. Scand J Immunol. 32:37-44, 1990 Tardivel S, Medetognon S, Randoux J, : Alpha-1-microglobulin: inhibitory effect on calcium oxalate crystallization in vitro and decreased urinary concentration in calcium oxalate stone formers. Urol Res. 27:243-49, 1999 Iida S, Peck AB, Bayer KJ, Khan SR: Expression of bikunin mRNA in renal epithelium cells after oxalate exposure. J Urol. 162:1480-6, 1999. Scheid C, Honeyman T, Kohjimoto Y, Cao LC, Jonassen J: Oxalate-induced changes in renal epithelial cell function: role in stone disease. Mol Urol. 4:371-382, 2000. Khan SR, Thamilselvan S: Nephrolithiasis: a consequence of renal epithelial cell exposure to oxalate and calcium oxalate crystals. Mol Urol. 4:371-382, 2000. Jonassen JA, Cooney R, Kennington L, : Oxalate-induced changes in the viability and growth of human renal epithelial cells. J Am Soc Nephrol 10:S446-S451, 1999. Miller C, Kennington L, Cooney R, : Oxalate toxicity in renal epithelial cells: characteristics of apoptosis and necrosis.Toxicol Appl Pharmacol. 162:132-41, 2000 Khan SR: Calcium oxalate crystal interaction with renal tubular epitherium, mechanism of crystal adhesion and its impact on stone development. Urol Res. 23: 71, 1995. Berggård T, Oury TD, Thogersen IB, : Alpha-1-microglobulin is found in blood and in most tissues. J Histochem Cytochem 46:887—93 1998. Takagi K, Kin K, Itoh Y, : Tissue distribution of human alpha-1-microglobulin. J Clin Invest 63:318-25, 1979 Bouic P, Vincent C, Revillard JP: Immunohistoloical localization of alpha-1-microglobulin in normal rat tissue. J Histochem Cytochem 21:717-23 1984. Bratt T, Olsson H, Sjöberg EM, : Cleavage of the alpha 1-microglobulin-bikunin precursor is localized to the Golgi apparatus of rat liver cells. Biochim Biophys Acta 1157:147-54, 1993 Sjöberg EM, Fries E: Biosynthesis of bikunin (urinary trypsin inhibitor) in rat hepatocytes. Arch Biochem Biophys 295:217—222, 1992 Lindstrom KE, Blom A, Johnsson E, : High glomerular permeability of bikunin despite similarity in charge and hydrodynamic size to serum albumin. Kidney Int 51:1053-8, 1997 Logberg LE, Akerstrom B, Badve S: Tissue distribution of the lipocalin alpha-1-microglobulin in the developing fetus. J Histochem Cytochem 48:1545-52, 2000 Yu H, Yanagisawa Y. Forbes MA: Alpha-1-microglobulin:an indicator protein for renal tubular function. J Clin Pathol 36:253-9, 1983. Itoh Y, Kawai T: Human alpha 1-microglobulin: its measurement and clinical significance. J Clin Lab Anal 4:376-384, 1990 Xu Y, Carr PD, Guss JM, : The crystal structure of bikunin from the inter-alpha-inhibitor complex: a serine protease inhibitor with two Kunitz domains. J Mol Biol 276:955-66, 1998 Atamani F, Lacour B, Strecher G, : Molecular characteristic of uronic-acid-rich protein, a strong inhibitor of calcium oxalate crystallization in vitro Biochem Biophys Res Commun 191:1158-65, 1993 Dean C, Kanellos J, Pham H, : Effects of inter-alpha-inhibitor and several of its derivatives on calcium oxalate crystallization in vitro. Clin Sci (Lond) 98:471-80, 2000
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