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研究生:簡士超
研究生(外文):Shih-Chao Chien
論文名稱:綠島產肥厚肉質與蜿蜒繖軟珊瑚化學成分及癌細胞毒殺活性之研究
論文名稱(外文):Chemical Constituents and Cytotoxicity of Formosan soft Corals Sarcophyton crassocaule an Xenia puerto-galerae
指導教授:杜昌益杜昌益引用關係
指導教授(外文):Cheng-Yeh Duh
學位類別:碩士
校院名稱:國立中山大學
系所名稱:海洋資源學系研究所
學門:自然科學學門
學類:海洋科學學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:中文
論文頁數:138
中文關鍵詞:癌症珊瑚
外文關鍵詞:Xenia puerto-galeraesoft CoralsSarcophyton crassocaulecancer
相關次數:
  • 被引用被引用:1
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  • 下載下載:34
  • 收藏至我的研究室書目清單書目收藏:1
綠島產肥厚肉質(Sarcophyton crassocule Von. Marenzeller)軟珊瑚之有機層萃取物經細胞活性測試,發現其對P-388、HT-29及A-549三種癌細胞有毒殺活性,以癌細胞毒殺活性為指標,再將二氯甲烷之萃取物以矽膠管柱層析進行分離純化後,得到四個cembrane diterpenoid化合物,分別是GN16-34 (1), GN16-35 (2), GN16-40 (3), GN16-62 (4),其中化合物4是新的新化合物。
綠島產蜿蜒繖(Xenia puerto-galerae Roxas)軟珊瑚之二氯甲烷層及丙酮層粗萃取物,以矽膠管柱層析及高效能液相色層分析儀(high-performance liquid chromatography, HPLC)進行分離純化後,得到六個cadinane sesquiterpenes化合物,分別為GN44-28 (5)、GN44-30 (6)、GN44-44 (7)、GN44-66 (8)、GN44-173 (9)、GN44-149 (10)及一個diterpenes,為GN44-199 (11),其中化合物5-10是新的合物。
上述化合物經癌細胞毒殺活性測試後,化合物3及4對P-388、HT-29及A-549細胞株均有顯著的抑制作用,化合物1及2對P-388細胞株有抑制作用,化合物5及7對A-549細胞株有顯著的抑制作用,化合物10對P-388及A-549細胞株均有抑制作用。
The methylene chloride extracts of Formosan soft coral Sarcophyton crassocule Von. Marenzeller (collected at Green Island off Taiwan) were found to exhibit significant cytotoxicity against P-388, HT-29, and A-549 cancer lines. Chromatographic separation led to the isolation of three known cembrane diterpenoids, GN16-34 (1), GN16-35 (2), GN16-40 (3), and a new cembrane, GN16-62 (4).
The methylene chloride and acetone extracts of Formosan soft coral Xenia puerto-galerae Roxas (collected at Green Island off Taiwan) were found to exhibit significant cytotoxicity against P-388 and A-549 cancer cell lines. Chromatographic separation resulted in the isolation of six new cadinane sesquiterpenes, GN44-28 (5), GN44-30 (6), GN44-44 (7), GN44-66 (8), GN44-173 (9), GN44-149 (10), and one known dicarbocyclic diterpenes with a bicyclic [4.3.1] ring system, GN44-199 (11).
Compounds 3 and 4 exhibited significant cytotoxicity against P-388, HT-29, and A-549 cancer cell lines. Compounds 1 and 2 exhibited significant cytotoxicity against P-388 cancer cell lines. Compounds 5 and 7 exhibited significant cytotoxicity against A-549 cancer cell line. compound 10 exhibited significant cytotoxicity against P-388 and A-549 cancer cell lines.
壹、緒論
一、前言
二、Sarcophyton屬軟珊瑚之天然物化學研究回顧
三、Xenia 屬軟珊瑚之天然物化學研究回顧
四、研究動機及目的
貳、肥厚肉質軟珊瑚化學成分之研究
一、材料採集與鑑定
二、肥厚肉質軟珊瑚之分類地位
三、肥厚肉質軟珊瑚之萃取與分離
四、肥厚肉質軟珊瑚化學成分之分離純化
(1)GN16-34 (1)之分離純化
(2)GN16-35 (2)之分離純化
(3)GN16-40 (3)之分離純化
(4)GN16-62 (4)之分離純化
五、肥厚肉質軟珊瑚化學成分之結構解析
(1)GN16-34 (1)之結構解析
(2)GN16-35 (2)之結構解析
(3)GN16-40 (3)之結構解析
(4)GN16-62 (4)之結構解析
參、蜿蜒繖軟珊瑚化學成分之研究
一、材料採集與鑑定
二、蜿蜒繖軟珊瑚之分類地位
三、蜿蜒繖軟珊瑚之萃取與分離
四、蜿蜒繖軟珊瑚化學成分之分離純化
(1)GN44-28 (5)之分離純化
(2)GN44-30 (6)之分離純化
(3)GN44-44 (7)之分離純化
(4)GN44-66 (8)之分離純化
(5)GN44-173 (9)之分離純化
(6)GN44-149 (10)之分離純化
(7)GN44-199 (11)之分離純化
五、蜿蜒繖軟珊瑚化學成分之結構解析
(1)GN44-28 (5)之解構解析
(2)GN44-30 (6)之解構解析
(3)GN44-44 (7)之解構解析
(4)GN44-66 (8)之解構解析
(5)GN44-173 (9)之解構解析
(6)GN44-149 (10)之解構解析
(7)GN44-199 (11)之解構解析
肆、化合物之細胞毒殺活性
伍、結論
陸、參考文獻
柒、附錄
一、儀器及材料
二、癌細胞毒殺活性測試之分析方法
三、MTT分析原理
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