臺灣博碩士論文加值系統

單細胞藻類可利用太陽能將二氧化碳固定成生物質量。因為其自營生長的特性，所以可供人類永續利用。目前大量培養藻類的方法是在大型開放池子中進行。單細胞藻類的生長限制因子主要為光照及二氧化碳濃度。然而傳統培養方法並無法克服上述的問題。為了提高產量、降低成本，必須找出克服生長限制因子的培養方法。
本研究開發新型光生化反應器來培養Nannochloropsis sp.。此反應器以直立網板為中心，使菌體液在網板上形成薄膜而流動。如此可克服因培養深度過深所造成光照路徑過長、光線分佈不均的問題。縮短培養時間，並提高產量。

目錄 I
圖目錄 III
表目錄 V
第一章 緒論 1
1-1 前言 1
1-2 研究目的 4
第二章 文獻回顧 7
2-1 藻類培養簡介 7
2-2 培養系統簡介 7
2-2-1 開放系統 8
2-2-2 密閉系統 9
第三章 實驗材料及方法 11
3-1 菌種 11
3-2 培養基 12
3-2-1 ASW100、ASW50 medium 12
3-2-2 I/55 medium 13
3-2-3 Artificial seawater medium 13
3-2-4 Proteose medium 14
3-2-5 Soil extract medium 14
3-4 靜置培養條件 23
3-5 光生化反應器裝置 23
第四章 分析方法 25
4-1 藻細胞濃度分析 25
4-1-1 血球計數器測量藻細胞濃度 25
4-1-2 分光光度計 25
4-2 氣相層析儀脂肪酸分析方法 26
4-2-1 樣品前處理 26
. 4-2-2 標準品製備 27
4-2-3 氣相層析儀設備及分析條件 27
第五章 實驗結果與討論 29
5-1 建立分析方法 29
5-1-1 藻細胞濃度分析 29
5-1-2 氣相層析儀脂肪酸分析 29
5-2 篩選菌種 33
5-3 培養條件研究 37
5-3-1 光照強度的影響 37
5-3-2 接菌量的影響 40
5-3-3 培養基的影響 43
5-4 光生化反應器設計 48
5-4-1 光生化反應器培養與平面培養的比較 48
5-4-2 光生化反應器光照條件的影響 52
第六章 結論與未來展望 56
6-1 結論 56
6-2 未來展望 57
參考文獻 59

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