跳到主要內容

臺灣博碩士論文加值系統

(44.210.132.31) 您好!臺灣時間:2022/08/19 19:00
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果 :::

詳目顯示

我願授權國圖
: 
twitterline
研究生:侯景祥
研究生(外文):Ching-Shiang Hou
論文名稱:台灣香魚族群遺傳結構之研究
論文名稱(外文):The Genetical Structure of Populations of Ayu (Plecoglossus altivelis) of Taiwan
指導教授:曾晴賢曾晴賢引用關係
指導教授(外文):Chyng-Shyan Tzeng
學位類別:碩士
校院名稱:國立清華大學
系所名稱:生命科學系
學門:生命科學學門
學類:生物學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:中文
論文頁數:55
中文關鍵詞:香魚族群遺傳結構移植親緣關係
外文關鍵詞:ayugenetic structuretransplantationphylogeny
相關次數:
  • 被引用被引用:4
  • 點閱點閱:873
  • 評分評分:
  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:2
香魚曾經是台灣重要的固有魚種,然而由於棲地被破壞與水壩的建立,在1968年便已絕跡;經過多年來的移植,香魚在1984年於北勢溪復育成功。本實驗的目的,是利用粒線體控制區前端340鹼基對的變異,以釐清目前台灣的香魚族群與日本種源之間的關係,並探討台灣香魚族群的遺傳結構。
實驗共採集本省北勢溪、大漢溪與大甲溪的香魚標本57尾、日本日置川與米代川標本39尾及中國福建省交溪香魚3尾。由親緣關係樹可以看到台灣的三條河川中均有來自日置川與米代川的香魚基因;族群間的淨核甘酸替代數目(dA)與基因互流量(Nm)的結果則顯示北勢溪的香魚族群主要來自日置川,大甲溪的香魚族群主要來自米代川,大漢溪族群則主要源自於移植到北勢溪的日置川香魚。
各族群間的單倍基因型多樣性指數都很高,平均為0.964;日本族群的單倍基因型多樣性指數(平均為0.989)又顯著高於台灣族群(平均為0.906);基因漂變與對環境的適應性演化,可能是造成台灣香魚族群基因歧異度降低的主要原因。
Ayu (Plecoglossus altivelis) was an important indigenous freshwater fish in Taiwan. But it was extinct since 1968 due to habitat destruction and blockage of migratory routes. At 1984, ayu was transplanted into Peishi river and have reproduced successfully. We investigated the first 340bp of mitochondrial control region to reveal the relationship between ayu in Taiwan and Japan, and the population genetical structure of ayu in Taiwan.
Fifty-seven samples were collected from Peishi, Tahan and Tachia river of Taiwan, 39 samples were collected from Hiki-gawa and Yoneshiro-gawa of Japan, and 3 samples were collected from Jiao river of Fu-chien, mainland China. The phylogenetic tree showed that the ayu populations of three rivers in Taiwan were composed both from Hiki-gawa and Yoneshiro-gawa. The result of population differentiation index (dA) and geneflow (Nm) indicate that the ayu population now in Peishi river were mainly from Hiki-gawa, the population in Tachia river were mainly from Yoneshiro-gawa and the population in Tahan river were mainly from Peishi river.
The genetic diversity index (h andπ) shows that the haplotype diversity in each population is high, and the average haplotype diversity of Japan population is significantly higher than Taiwan population . Genetic drift and adaptive evolution maybe the main reasons responsible for the reduction in genetic variation of ayu population of Taiwan.
目 錄
摘要...................................I
Abstract...............................Ⅱ
謝誌..................................III
壹.前言
一﹑香魚的生態及分類...................1
二﹑台灣的香魚.........................2
三﹑分子生物技術的應用.................3
四﹑文獻回顧...........................5
五﹑研究動機...........................7
六﹑研究目的與方法.....................7
貳.材料與方法
一﹑標本採集...........................8
二﹑形態比較...........................8
三﹑DNA序列分析........................8
參. 結果
一﹑形質比較...........................16
二﹑粒線體控制區序列變異分析...........18
三﹑親緣關係樹的重建...................22
四﹑族群間遺傳分化程度與族群遺傳結構...23
肆. 討論
一﹑粒線體控制區序列組成...............25
二﹑族群之親緣關係樹 ..................25
三﹑香魚之族群遺傳結構分析.............27
四﹑台灣香魚的種源推測.................30
五﹑移植問題的探討.....................30
伍. 結論...............................32
陸. 引用文獻...........................33
圖.....................................38
表.....................................50
附錄...................................55
Allendorf, F. W. and Ryman, N. (1987) Population Genetics and Fishery Management. pp. 141-159, University of Washington Press, Seattle.
Avise, J. C. (1989) Gene trees and phylogenetic approach to population biology. Evol. 13:1192-1208.
Avise, J. C. (1994) Molecular markers, Natural History and Evolution. Chapman & Hall, New York.
Brown, W. M., George, M. J. and Wilson. A. C. (1984). Polymorphic sites and mechanism of evolution in human mitochondria DNA. Genetics 106:479-499.
Brown, W. M. (1985) The mitochondrial genome of animals. Molecular Evolutionary Genetics, Plenum., New York.
Chang, Y. S., Huang, F. L. and Lo, T. B. (1994) The complete nucleotide sequence of carp (Cyprinus carpio) mitochondrial genome. J. Mol. Evol. 38:138-155.
Crozier, W. W. (1993) Evidence of genetic interaction between escaped farmed salmon and wild Atlantic salmon (Salmo salar L.) in a Northern Irish river. Aquaculture 113:19-29.
Crozier, W. W. (1994) Maintenance of genetic variation in hatchery stocks of Atlantic salmon, Salmo salar L.: experiences from the river bush, northern Ireland. Aquaculture and fisheries management 25:383-392.
Danzman R. G., Ferguson, M. M. and Allendorf, F. W. (1989) Genetic variability and components of fitness in hatchery strains of rainbow trout. Journal of fish biology 35:313-319.
Einum, S. and Fleming, I. A. (1997) Genetic divergence and interactions in the wild among native, farmed and hybrid Atlantic salmon. Journal of Fish Biology 50:634-651.
Hartl, D. L. and Clark, A. G. (1989) Principle of population genetics. Sinauer Associates Press, U.S.A.
Hoelzel, A. R., Hancock, J. M. and Dover, G. A. (1991) Evolution of the cetacean mitochondrial D-loop region. Mol. Biol. Evol. 8:475-493.
Iguchi, K. and Ito, F. (1994) Occurrence of cross-mating in ayu: amphidromous x land-locked forms, and diploid x triploid. Fisheries Science, 60:653-655.
Iguchi, K. and Yamaguchi (1994) Adaptive significance of inter- and intrapopulational egg size variation in ayu Plecoglossus altivelis (Osmeridae). Copeia 1994:184-190
Iguchi, K., Tanimura, Y. and Nishida, M. (1997) Sequence divergence in the mtDNA control region of amphidromous and landlocked forms of ayu. Fisheries Science 63:901-905.
Iguchi, K., Watanabe, K. and Nishida, M. (1999) Reduced mitochondrial DNA variation in hatchery populations of ayu (Plecoglossus altivelis) cultured for multiple generations. Aquaculture 178:235-243.
Innis, M. A., Gelfand, D. H., Sninsky, J. J. and White, T. J. (1989) PCR protocols: A guide to methods and applications. Academic Press Inc.
Jean, C. T., Lee, S. C., Hui, C. F. and Chen, C. T. (1995) Phylogenetic relationships of fishes of the subfamily Sparinae (Perciformes: Sparidae) from the costal waters of Taiwan. J. Zool. Syst. Evol. Res. 33:49-53.
Kawanabe, H. (1976) A note on the territoriality of ayu, Plecoglossus altivelis Temminck et Schlegel (Pisces: Osmeridae) in the Lake Biwa stock, based on the “relic social structure” hypothesis. Physiol. Ecol. Japan 17:395-399.
Kumar, S., Tamura K., Jakobsen I. B. and Nei, M. (2001) MEGA2: Molecular evolutionary genetics analysis software. Bioinformatics (submitted).
Lynch, M. and Crease, T. J. (1990) The analysis of population survey data on DNA sequence variation. Mol. Biol. Evol. 7:377-394.
Mignotte, B., Dunon-Bluteau, D., Reiss, C. and Mounolou, J. (1987) Sequence deduced physical properties in the D-loop region common to five vertebrate mitochondrial DNAs. J. Theor. Biol. 140:57-69.
Mullis, K. B. and Faloona, F. A. (1987) Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods Enzymol 155:335-350.
Nei, M. (1987) Molecular Evolutionary Genetics. Columbia University Press, New York.
Nelson, J. S. (1994) Fishes of the world, John Wiley & Sons, Inc., New York.
Nishida, M. (1985) Sexual difference in morphological characters in ayu, Plecoglossus altivelis (Pisces: Osmeridea). Bulletin of the College of Science 40:127-136.
Nishida, M. (1986) Geographic variation in the molecular, morphological and reproductive characters of the ayu Plecoglossus altivelis (Plecoglossidae) in the Japan-Ryukyu Archipelago. Japan. J. Ichthyol. 33:232-248.
Nishida, M. and Sawashi, Y. (1987) Geographic differences in the number of longitudinal scales of the ayu Plecoglossus altivelis. Nippon Suisan Gakkaishi 53:2089.
Nishida, M. (1988a) A new subspecies of the Ayu, Plecoglossus altivelis, (Plecoglossidae) from the Ryukyu Islands. Japan. J. Ichthyol. 35:236-242.
Nishida, M. (1988b) Differences in reproductive and morphometric characters in Lake Biwa and amphidromous forms of the ayu Plecoglossus altivelis : A comparison of fish transplanted into the same stream. Bulletin of the College of Science 48:69-75.
Oohara, I., Sawano K. and Okazaki, T. (1997) Mitochondrail DNA sequence analysis of the masu salmon — phylogeny in the genus Oncorhynchus. Molecular phylogenetics and evolution 7:71-78.
Rozas, J. and Rozas, R. (1999) DnaSP version 3: an integrated program for molecular population genetics and molecular evolution analysis. Bioinformatics 15:174-175.
Ryman, N. (1983) Patterns of distribution of biochemical genetics variation in salmonids: differences between species. Aquaculture 33:1-21
Ryman, M. and Utter, F. (1986) Population genetics and fishery management. pp. 1-18, University of Washington Press, Seattle and Landon.
Saavedra, C. and Guerra, A. (1996) Allozyme heterozygosity, founder effect and fitness traits in a cultivated population of the European oyster, Ostrea edulis. Aquaculture 139:203-224.
Saunders, N. C., Kessler, L. G. and Avise, J. C. (1986) Genetic variation and geographic differentiation in mitochondrial DNA of the horseshoe crab, Limulus polyphemus. Genetics 112:613-627.
Seki S., Taniguchi, N. and Jeon, S. (1992) Genetic divergence among natural populations of ayu from Japan and Korea. Nippon Suisan Gakkaishi 54:559-568.
Takagi, N., Shoji, E. and Taniguchi, N. (1999) Microsatellite DNA polymorphism to reveal genetic divergence in ayu, Plecoglossus altivelis. Fisheries Science 65:507-512.
Tamura, K. and Nei, M. (1993) Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol. Biol. Evol. 10:512-526.
Tzeng, C. S., Hui, C. F., Shen, S. C. and Huang, P. C. (1992) The complete nucleotide sequence of the Crossostoma lacustre mitochondrial genome: Conservation and variations among vertebrates. Nucleic Acids Res. 20:4853-4858.
Verspoor, E. (1988) Reduced genetic variability in first generation populations of Atlantic salmon (Salmo salar). Canadian Journal of Fisheries and Aquatic Sciences 45:1686-1690.
Ward, R.D. and Grewe, P. (1994) Appraisal of molecular genetic techniques in fisheries. Reviews in Fish Biology and Fisheries 4:300-325.
Wilson, A. C., Cann, R. L., Carr, S. M., George, M., Gyllensten, U. B., Helmbychowski, K. M., Higuchi, R. G., Palumbi, S. R., Prager, E. M., Sage, R. D. and Stoneking, M. (1985) Mitochondrial DNA and two perspective on evolutionary genetics. Biological Journal of the Linnean Society 26:375-400.
Wenink, P. W., Baker, A. J. and Tilanus, M. G. J. (1993) Hypervariable control-region sequences reveal global population structuring in a long-distance migrate shorebird, the Dunlin (Calidris alpina). Proc. Natl. Acad. Sci. USA 90:94-98.
Wright, S. (1951) The genetical structure of populations. Ann. Eugenics. 15:323-354.
王昱人,1997,台灣鉤吻鮭與日本櫻花鉤吻鮭遺傳多樣性之研究。清華大學生命科學系碩士論文。新竹。
江莎菲,1954,大甲溪的香魚。漁友半月刊,36:20-21。
英木,1954,香魚人工孵化。漁友半月刊,26:21-22。
青木赳雄,1915,鮎人工孵化試驗報告。台灣水產雜誌,4:9-29。(日文)。
湯弘吉,1988,台灣之香魚。生物科學,31:39-45。
湯弘吉,1993,台灣香魚資源之復育及管理。農政與農情,34:34-40。
湯弘吉、彭弘光、余廷基,1987,香魚的人工繁殖試驗。台灣省水產試驗所報告,43:153-158。
鈴木敬二,1984,台湾のアユの滅絶とその復活。淡水魚,105-108。(日文)。
廖德裕,2000,台灣產埔里華吸鰍族群間分子親緣關係之研究。清華大學生命科學系碩士論文。新竹。
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top