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研究生:詹馥菱
研究生(外文):Fu-Ling Chan
論文名稱:青螺造牙組織的礦化研究
論文名稱(外文):The biomineralization of the radula in Patelloida striata
指導教授:李家維李家維引用關係
指導教授(外文):Chi-Wei Li
學位類別:碩士
校院名稱:國立清華大學
系所名稱:生命科學系
學門:生命科學學門
學類:生物學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:中文
論文頁數:49
中文關鍵詞:青螺齒舌生物礦化
外文關鍵詞:limpetradulabiomineralization
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細線青螺(Patelloida striata)在分類上屬於軟體動物門、腹足綱、原始腹足目中的青螺科,俗稱 limpet。青螺具有羽狀鰓,背殼為斗笠形,內面常呈青白色沒有珍珠光澤,邊緣常有深色環。青螺生活在岩礁海岸的潮間帶,利用鐵與矽礦化的齒舌刮取岩石上的藻類為生。在青螺齒舌上不同階段的牙具有不同的礦化程度,呈現出線性的動態平衡,是觀察生物礦化過程的絕佳材料。
在牙冠與牙基交界處稱為junction zone,利用光學解剖顯微鏡、穿透式電子顯微鏡以及掃描式電子顯微鏡觀察這個區域,發現junction zone在stage II的初期即有鐵的沉積,一直到成熟的牙中仍有鐵的沉積存在。透過普魯士藍染色及電子繞射的結果顯示,在這個區域的鐵為非結晶型態的沉積,因此推測此部位具有特殊的生理功能,可能在當牙被剝除或損壞時,可保護牙基與其下的口部肌肉。
在感應耦合電漿質譜分析的研究中,發現矽與鐵已出現在stage I的周邊細胞中,此時的鐵無法用普魯士藍進行標定,在電子顯微鏡下亦無鐵結晶的蹤跡,而矽也少量出現在stage I的牙冠中。鐵在stage II開始大量沉積到牙腔中,矽則到了stage III才開始快速地沉積下來。在stage IV的周邊細胞中發現含有大量的矽與鐵,可能表示著青螺齒舌中具有回收矽與鐵的系統。
另外,找到三個分子量介於15到20kDa的蛋白質,同時存在於周邊細胞與牙冠中,可能與矽或鐵的礦化相關。根據多株抗體的免疫染色結果,推論這些蛋白質可能屬於膜蛋白或經過修飾後送到牙腔中的分泌蛋白。

Limpets are mollusks of the intertidal and subtidal regions whose radula possess teeth hardened by biominerals. The radula in limpets is a ribbon-like apparatus used for feeding and which bears a series of distinctive mineralized teeth. The radula of the limpet, Patelloida striata, consists of a continuous series of teeth in various stages of iron and silica biomineralization.
The morphology of the radular teeth of the limpet have been examined using light, transmission and scanning electron microscopy, together with energy dispersive X-ray analysis spectroscopy. The initial deposition of iron in the teeth takes place in the junction zone, the region that lies between the tooth cusp and the tooth base. Iron in the junction zone is amorphous form, and remains as the teeth becomes matured. The junction zone may have specific physiological functions, such as protection of the tooth base and muscle in lower when the teeth are broken down.
In addition, three peptides with molecular size between 15 and 20 kDa were found in cells, as well as in the tooth cusps. These peptides may involve in biomineralization. According to the result of poly-cloned antibodies detection, these peptides could be membrane proteins or secretory proteins that are transported into cusp after modification.

摘要---i
誌謝---iii
目錄---iv
一、簡介---1
1-1.生物礦化---1
1-2.矽藻外殼的礦化研究---2
1-3.海綿骨針的研究---4
1-4.軟體動物的齒舌研究---5
1-5.總結---6
二、實驗材料與方法---7
2-1.實驗材料---8
2-2.實驗材料初步觀察---8
2.3.組織切片及染色---8
2-4.掃描式電子顯微鏡---9
2-5.外加能量分散光譜儀的觀察---9
2-6.穿透式電子顯微鏡的觀察---10
2-7.感應耦合電漿質譜分析儀---11
2-8.牙腔與周圍蛋白質的萃取---11
2-9.電泳分析---12
2-10.免疫染色---12
2-11.高效率液相層析儀---13
三、結果---14
3-1.外觀觀察---14
3-2.組織切片觀察---15
3-3.掃描式電子顯微鏡觀察---15
3-4.外加能量分散光譜儀的觀察---16
3-5.穿透式電子顯微鏡的觀察---16
3-6.ICP-MS分析不同階段的矽、鐵含量---17
3-7.牙腔與周圍蛋白質的萃取與分析---17
3-8.免疫染色---18
3-9.目標蛋白的純化與確認---19
四、討論---20
4-1.鐵的沉積---20
4-2.齒舌的元素含量分析---21
4-3.蛋白質純化與分析---22
五、參考文獻---24
六、表格---27
七、圖片---31

1.Riding, R. (1986) Biomineralization in Lower Plants and Animals. (Leadbeater, B.S.C., and Riding, R., eds.), Oxford University Press, New York.
2.Lowenstam, H.A., and Weiner, S. (1989) On Biomineralization. Oxford University Press, New York.
3.戴永定 等著, (1994) 生物礦物學. 石油工業出版社.
4.Kröger, N., Lehmann, G., Rachel, R., and Sumper, M. (1997) Characterization of a 200-kDa diatom protein that is specifically associated with a silica-based substructure of the cell wall, Eur. J. Biochem. 250, 99-105.
5.Round, F.E., and Crawford, R.M. (1990) Phylum Bacillariophyta, Handbook of protoctista. (Margulis, L., Corliss, J.O., Melkonian, M., and Chapman, D.J., eds.), Jones and Bartlett Publishers, Boston.
6.Kröger, N., Bergsdorf, C., and Sumper, M. (1994) A new calcium binding glycoprotein family constitutes a major diatom cell wall component, EMBO J. 13, 4676-4683.
7.Coombs, J., Halicki, P.J., Holm-Hansen, O., and Volcani, B.E. (1967) Studies on the biochemistry and fine structure of silica shell formationin diatoms. Experimental Cell Research. 47, 315-328.
8.Kröger, N., Bergsdorf, C., and Sumper, M. (1996) Frustulins: domain conservation in a protein family associated with diatom cell walls, Eur. J. Biochem. 239, 259-264
9.Kröger, N., Deutzmann, R., and Sumper, M. (1999) Polycationic peptides from diatom biosilica that direct silica nanosphere formation, Science. 286, 1129-1132.
10.Kröger, N., Deutzmann, R. Bergsdorf, C., and Sumper, M. (2000) Species-specific polyamines from diatoms control silica morphology, Proc. Natl. Acad. Sci. 97, 14133-14138.
11.Kröger, N., Deutzmann, R., and Sumper, M. (2001) Silica-precipitating peptides from diatoms, J.B.C. 276, 26066-26070.
12.Shimizu, K., Cha, J., Stucky, G.D., and Morse, D.E. (1998) Silicatein α: Cathepsin L-like protein in sponge biosilica, Proc. Natl. Acad. Sci. 95, 6234-6238.
13.台灣貝類資料庫http://shell.sinica.edu.tw/
14.Rinkevich, B. (1993) Major primary stages of biomineralization in radular teeth of the limpet Lottia gigantean, Marine biology. 117, 269-277.
15.Evans, L.A., Macey, D.J., and Webb, J. (1990) Characterization and structural organization of the organic matrix of the radula teeth of the chiton Acanthopleura hirtosa, Phil.Trans. R. Soc. Lond. B 329, 87-96.
16.Mann, S., Perry, C.C., Webb, J., Luke, B., and Williams, R.J.P. (1986) Structure, morphology, composition and organization of biogenic minerals in limpet teeth, Proc. R. Soc. Lond. B 227, 179-190.
17.呂學冠, (1995) 花笠螺齒舌的礦化研究, 國立清華大學博士論文.
18.Macey, D.J., and Brooker, L.R. (1996) The junction zone: initial site of mineralization in radula teeth of the chiton Cryptoplax striata (mollusca: polyplacophora), Journal of Morphology. 230, 33-42.
19.Burford, M.A., Macey, D.J., and Webb, J. (1986) Hemolymph ferritin and redula structure in the limpets Patelloida alticostata and Patella peronii (Mollusca: Gastropoda), Comp. Biochem. Physiol. 83A, 353-358.
20.Lu, H.-K., Huang, C.-M., and Li, C.-W. (1995) Translocation of ferritin and biomineralization of goethite in the radula of the limpet Cellana toreuma reeve, Experimental cell research. 219, 137-145.
21.Bancroft, J.D., and Steevens, A. (1990) Theory and Practice of Histological Techniques. 3rd ed. pp108. Longman Group UK Limited.

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