跳到主要內容

臺灣博碩士論文加值系統

(44.200.171.74) 您好!臺灣時間:2022/08/12 19:22
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果 :::

詳目顯示

我願授權國圖
: 
twitterline
研究生:李慧貞
研究生(外文):Hui-Cheng Lee
論文名稱:後突觸質密區中新鑑定的蛋白質-PSD500之研究
論文名稱(外文):Identification and characterization of a novel protein-PSD500 in the postsynaptic densities
指導教授:張兗君
指導教授(外文):Yen-Chung Chang
學位類別:碩士
校院名稱:國立清華大學
系所名稱:生命科學系
學門:生命科學學門
學類:生物學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:中文
論文頁數:62
中文關鍵詞:後突觸質密區PSD500dynein
外文關鍵詞:PSDPSD500dynein
相關次數:
  • 被引用被引用:1
  • 點閱點閱:124
  • 評分評分:
  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:0
哺乳動物中樞神經中的突觸,為一種連接兩個神經細胞的特殊構造,後突觸質密區(postsynaptic density,簡稱PSD)位在突觸的末端,這群巨分子蛋白質群約包含30個左右的蛋白質,在立體空間中這些蛋白質有著嚴謹的排列及組成,使得PSD在突觸中扮演著重要調控的角色,因此,進一步瞭解PSD中的蛋白質組成,將有助於了解中樞神經中訊息傳遞過程。雖然如此,由於分析上的限制,PSD的蛋白質組成尚未完全被鑑定出來。我們實驗室利用3.5 % Weber & Osborn電泳膠片分析PSD中的蛋白質,於490±10 kDa發現一個蛋白質帶,命名為PSD500,該蛋白質占PSD蛋白質總量的3.5±0.3 %,由subcellular fraction及組織分布發現PSD500豐富存在於腦中的PSD,透過Concancavlin A-conjugated peroxidase的染色,PSD500可能是一個含有少量醣基的蛋白質,但以peptide N-glycosidase F(簡稱PNGF)去除醣基作用所造成PSD500分子量的改變並無法由3.5 % Weber & Osborn電泳膠片看出,因此PSD500上的醣基可能並非是N-linked glycans或此種分析系統無法觀察到少量分子量的差異。由MALDI-MS的結果,經由MS-Fit搜尋得到6個蛋白質名稱,其中兩個為b dynein heavy chain,四個為cytoplasmic dynein heavy chain,因此,我們初步認為PSD500可能為dynein heavy chain。

摘要
壹、緒論………………………………………………………………….1
貳、材料與方法…………………………………………………………10
(一) 藥品與方法…………………………………………………...10
(二) PSD的純化……………………………………………………11
(三) 凝膠電泳分析(SDS-PAGE)………………………………13
(四) 西方點漬法(Western Blot)………………………………...15
(五) 以Electroelution純化PSD-500……………………………...16
(六) DTE/IAA修飾所有雙硫基…………………………………...17
(七) 多株抗體(polyclonal antibody)製備………………………18
(八) 穩定度測定…………………………………………………...20
(九) 去醣基作用…………………………………………………...21
(十) Con A-conjugated peroxidase染色…………………………...21
(十一) In Solution Digest PSD500 for MALDI-MS……………….22
(十二) 氯仿-甲醇沈澱法………………………………………...23
(十三) 蛋白質濃度測定…………………………………………...23
(十四) 膠片染色法………………………………………………...24
參、結果
(一) PSD中的PSD500……………………………………………..25
(二) PSD500的分佈………………………………………………..26
(三) PSD500是否為一個醣蛋白…………………………………..27
(四) PSD500的穩定性……………………………………………..28
(五) 以MALDI-MS鑑定PSD500…………………………………28
肆、討論…………………………………………………………………30
伍、參考文獻…………………………………………………………...48
Appendix. I. The apparatus of Electroelution…………………………...58
Appendix. II. Useful Mass Spectrometry Web Site……………………..59
Appendix. III. Useful MALDI-MS fingerprint map web site ………….60
Appendix. IV. PSD500 are interior proteins within the PSD …………..61
Appendix. V. Scheme of KIFs and cytoplasmic dynein and their cargo organelles in nerve axons……………………………………………….62

Apperson, M. L., Moon, I. S., and Kennedy, M. B. (1996) Characterization of densin-180, a new brain-specific synaptic protein of the O-sialoglycoprotein family. J. Neurosci. 16, 6839-6852.
Barria, A., Muller, D., Kerkach, V., Griffith, L. C. and Soderling, T. R. (1997) Regulatory phosphorylation of AMPA-type glutamate receptors by CaMKII during long term potentiation. Science. 276, 2042-2045.
Bessley, P. W., Mummery, R. and Tibaldi, J. (1995) N-cadherin is a major glycoprotein component of isolated rat forebrain postsynaptic densities. J. Neurochem. 64,2288-2294.
Blackstone, C. D., Moss, S. J., Martin, L. J., Levey, A. I., Price, D. L., and Hunganir, R. L. (1992) Biochemical characterization and localization of a non-N-Methyl-D-Aspartate glutamate receptor in rat brain. J. Neurochem. 58, 1118-1126.
Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72,248-254.
Bredt, D. S., Hwang, P. H. and Snyder, S. H. (1990) Localization of nitric oxide synthase indicating a neural role for nitric oxide. Nature. 347, 768-770.
Brenman, J. E., Chao, D. S., Gee, S. H., McGee, A. W., Craven, S. E., Santillano, D. R.,Wu, Z., Huang, F., Xia, H., Peters, M. F., Froehner, S. C. and Bredt, D. S. (1996) Interaction of nitric oxide synthase with the postsynaptic density protein PSD-95 and alpha 1-syntrophin mediated by PDZ domains. Cell. 84, 757-767.
Butz, S., Okamoto, M. and Sudhof, T. C. (1998) A tripartite protein complex with the potential to couple synaptic vesicle exocytosis to cell adhesion in brain. Cell. 94, 773-782.
Caceres, A., Binder, L. T., Payne, M. R., Bender, P.,Rebhun, L. and Steward, P. (1984) Differential subcellular localization of tubulin and the microtubule associated protein MAP2 in brain tissue as revealed by immunocytochemistry with monoclonal hybridoma antibodies. J. Neurosci. 4, 394-410.
Caceres, A., Payne, M. R., Binder, L. I. and Steward, O. (1983) Immnocytochemical localization of actin and microtubule- associated protein MAP2 in dendritic spines. Proc. Natl. Acad. Sci. USA. 80,1738-1742.
Carlin, R. K. and Siekevita, P. (1984) Characterization of Na+-independent of GABA and flunitrazepam binding sites in preparations of synaptic membranes and postsynaptic densities from canine cerebral cortex and cerebellum. J. Neurochem. 43, 1011-1017.
Carlin, R. K., Bartelt, D. C. and Siekevitz, P. (1983) Identification of fordrin as a major calmodulin-binding protein in postsynaptic density preparation. J. Cell Biol. 96, 443-448.
Carlin, R. K., Grab, D. J., Cohen, R. S. and Siekevitz, P. (1980) Isolation and characterization of postsynaptic densities from various brain regions: enrichment of different types of postsynaptic densities. J. Cell Biol. 86, 831-843.
Carr, D. W., Stofko, H. R., Fraser, J. D., Cone, R. D. and Scott, J. D. (1992) Localization of the cAMP-dependent protein kinase to the postsynaptic densities by A-kinase anchoring proteins. Characterization of AKAP 79. J. Biol. Chem. 267, 16818-16823.
Cho, K-O., Hunt, C. A. and Kennedy, M. B. (1992) Thr rat brain postsynaptic density fraction contains a homolog of the Drosophila discs-large tumor suppressor protein. Neuron. 9, 929-942.
Cohen, R. S. and Siekevitz, P. (1978) Form of the postsynaptic density: A serial section study. J. Cell. Biol. 78, 36-46.
Cohen, R. S., Blomberg, F., Berzin, K. and Siekevitz, P. (1977) The structure of postsynaptic densities isolated from dog cerebral cortex I: overall morphology and protein composition. J. Cell. Biol. 74, 181-203.
Colonnier, M. (1968) Synaptic patterns on different cell types in the different laminae of the cat visual cortex. An electron microcopy study. Brain Res. 9, 268-287.
Cotman, C. W., Banker, G., Churchill, L., and Taylor, D. (1974) Isolation of postsynaptic densities from rat brain. J. Cell. Biol. 63, 441-455.
Dong, H., O’Brien, R. J., Fung, E. T., Lanahan, A. A., Worley, P. F. and Huganir, R. L. (1997) GRIP: a synaptic PDZ domain-containing protein that interacts with AMPA receptors. Nature. 386, 279-284.
Dosemeci, A. and Resse, T. S. (1995) Effect of calapin on the composition and structure of postsynaptic density. Synapse. 20, 91-97.
Dosemsci, A. and Reese, T. S. (1993) Inhibition of enogenous phosphatase in a postsynaptic density fraction allows extensive phosphorylation of the major postsynaptic density protein. J. Neurochem. 61, 550-555.
Ewards, F. (1991) Neurobiology-LTP is a long term problem. Nature. 350, 271-272.
Faddis, B. T., Hasbani, M. J. and Goldberg, M. P. (1997) Calpain activation contributes to dendritic remodeling after brief excitotoxic injury in vitro. J. Neurosci. 17, 951-959.
Fagg, G. E. and Matus, A. (1984) Selective association of N-methyl-D-aspartate and quisqualate type of L-glutamate receptor with brain postsynaptic densities. Proc. Natl. Acad. Sci. USA. 81, 6876-6880.
Finkbeiner, S. and Greenberg, M. E. (1996) Ca2+-dependent routes to Ras: mechanisms for neuronal survival, differentiation and plasticity? Neuron. 16, 223-236.
Fukunaga, K., Goto, S. and Miyamoto, E. (1988) Immnohistochemical localization of Ca2+/calmodulin-dependent protein kinase II in rat brain and various tissues. J. Neurochem. 51, 1070-1078.
Furukawa, K., Fu, W., Li, Y., Witke, W., Kwiatkowski, D. J. and Mattson, M. P. (1997) The actin-servering protein gesolin modulates calcium channel and NMDA receptors activities and vulnerability to excitotoxicity in hippocampal neurons. J. Neurosci. 17, 8187-8186.
Geinsman, Y., deToledo-Morrell, L. and Morrell, F. (1991) Induction of long-term potentiation is associated with an increase in the number of azospinous synapses with segmented postsynaptic densities. Brain Res. 566, 77-88.
Goldenring, J. R., Mcguire, J. J. and Delorenzo, R. J. (1984b) Identification of the mafor postsynaptic density protein as homologous with the mafor calmodulin-binding subunit of a calmodulin-dependent protein kinase. J. Neurochem. 42, 1077-1084.
Goldernring, J. R., Casanova, J. E. and Delorenzo, R. J. (1984a) Tubulin-associated calmodulin-dependent protein kinase: evidence for an endogenous complex of tubulin with a calcium- calmodulin- dependent kinase. J. Neurochem. 43, 1669-1679.
Gomperts, S. N. (1996) Clustering membrane protein: it’s all coming together with the PSD-95/SAP90 protein family. Cell. 84, 659-662.
Gray, E. G. (1959) Axo-somatic and axo-dendritic synapses of the cerebral cortex: an electron microscope study. J. Anat. 93, 420—433.
Harris, A. S., Croall, D. E. and Morrow, J. S. (1998) The calmodulin-binding site in near the calcium-dependent protease-I cleavage site. J. Biol. Chem. 263, 15754-15761.
Harris, K. M. and Kater, S. B. (1994) Dendritic spines: cellular specializations imparting both stability and flexibility to synaptic function. Annu. Rev. Neurosci. 17, 341-371.
Hayashi, Y., Shi, S. H., Esteban, J. A., Piccini, A., Poncer, J. C., Malinow, R. (2000) Driving AMPA receptors into synapses by LTP and CaMKII: requirement for GluR 1 and PDZ domain interaction. Science. 287, 2262-2267.
Hering, H. and Sheng, M. (2001) Dendritic spines: structure, dynamics and regulation. Nat. Rev. Neurosci. 2, 880-888.
Hirokawa, N. (1998) Kinesin and dynein superfamily proteins and the mechanism of organelle transport. Science. 279, 519-526.
Hollmann, M. and Heinemann, S. Cloned glutamate receptors. Annu. Rev. Neurosci. 17, 31-108.
Hsueh, T. P., Kim, E. and Sheng, M. (1997) Disulfide-linked head-to-head multimerization in the mechanism of ion channel clustering by PSD-95. Neuron. 18, 803-814.
Husi, H. and Grant, S. G. N. (2001) Proteomics of the nervous system. Trends in Neurosci. 24, 259-266.
Husi, H., War, M. A., Choudhary, J. S., Blackstock, W. P. and Grant, S. G. N. (2000) Proteomic analysis of NMDA receptor-adhesion protein signaling complexes. Nat. Neurosci. 3,661-669.
Ichtchenko, K. Hata, T., Nguyen, T., Ullrich, B. Missler, M., Moomaw, C., and Sudhof, T. C. (1995) Neuroligin 1: a splice site-specific ligand for beta-neurexins. Cell. 81, 435-443.
Irie, M., Heta, Y., Takeuchi, M., Ichtchnko, K., Toyoda, A., Hirao, K., Takai, Y., Rosahl, T. W. and Sudhof, T. C. (1997) Binding of neuroligins to PSD-95. Science. 277, 1511-1515.
Jensen, O. N., Podtelejnikov, A. V. and Mann, M. (1997) Identification of the components of simple protein miztures by high-acuracy peptide mass mapping and detabase searching. Anal. Chem. 68, 850-858.
Kaech, S. M., Whitfield, C. W. and Kim, S. K. (1998) The Lin-2/Lin-7/ Lin-10 complex mediates basolateral membrane localization of the C. elegans EGF receptor LET-23 in vulval epithelial cells. Cell. 94, 761-771.
Kelly, P. T. and Cotman, C. W. (1978) Characterization of tubulin and actin and identification of a distinct postsynaptic density polypeptide. J. Cell. Biol. 79, 173-183.
Kennedy, M. B. (1997) The postsynaptic density at glutamatergic synapses. Trends Neurosci. 20, 264-268.
Kennedy, M. B., Bennett,M. K. and Erondu, N. E. (1983) Biochemical and immunochemical evidence that the “major postsynaptic density protein” is a subunit of a calmodulin-dependent protein kinase. Proc. Natl. Acad. Sci. USA. 80, 7351-7361.
Kim, E., Cho, K. O., Rothschild, A. and Sheng, M. (1996) Heteromultimerization and NMDA receptor-clustering activity of chapsyn-110, a member of the PSD-95 family of proteins. Neuron. 17, 103-113.
Kim, J. H. and Huganir, R. L. (1999) Organization and regulation of proteins at synapses. Curr. Opin. Cell Biol. 11, 248-254.
Kim, T. W., Wu, K., Xu, J. L. and Black, I. B. (1992) Detection of dystrphin in the postsynaptic density of rat brain and deficiency in a mouse model of Duchenne muscular dystrophy. Proc. Natl. Acad. Sci. USA. 89,11624-11644.
Kistner, U., Wenzel, B. M., Beh, R. W., Cases-Langhoff, C., Garner, A. M., Appeltauer, U., Voss, B., Gundelfinger, E. D. and Garner, C. C. (1993) SAP90, a rat presynaptic protein related to the product of the Drosophila tumor suppressor gene dlg-A. J. Biol. Chem. 268, 4580-4583.
Kornau, H. C., Schenker, L. T., Kennedy, M. B. and Seeberg, P. H. (1995) Domain interaction between NMDA receptor subunits and the postsynaptic density protein PSD-95. Science. 269, 680-685.
Lacey, M. L., and Haimo, L. T. (1992) Cytoplasmic dynein is a vesicle protein. J. Biol. Chem. 267, 4793-4798.
Laemmli, U. K. (1970) Cleavage of structure proteins during assembly of the head of the bacteriophage T4. Nature. 227, 680-685.
Landis, D. M., Hall, A. K., Weinstein, L. A. and Reese, T. S. (1988) The organization of cytoplasm at the presynaptic active zone of a central nervous system synapse. Neuron. 1, 201-209.
Lau, L. F., Mammer, A., Ehlers, M. D., Kindler, S., Chung, W. J., Garner, C. C. and Huganir, R. L. (1996) Interaction of the N-methyl-D-aspartate receptor complex with a novel synapse-associated protein, SAP102. J. Biol. Chem. 271, 21622-21628.
Lidov, H. G. W., Byers, T. J., Watkins, S. C. and Kunkel, L. M. (1990) Localization of dystrophin to postsynaptic regions of central nervous system in sortical neurons. Nature. 348, 725-728.
Lin, C. T., Dedman, J. R., Brinkly, B. R. and Means, C. S. (1980) Localization of calmodulin in rat cerebellum by immunoelectron microscopy. J. Cell Biol. 87, 346-359.
Lücher, C., Nicoll, R. A., Malenka, R. C., and Muller, D. (2001) Synaptic plasticity and dynamic modulation of the postsynaptic membrane. Nat. Neurosci. 3, 545-549.
Monahan, J. B. and Michel, J. (1987) Identification and characterization of an N-methyl-D-aspartate-specific L-[3H]glutamate recognition site in synaptic plasma membranes. J. Neurochem. 48, 1699-1708.
Muller, B. M., Kistner, U., Beh, R. W., Cases, L. C., Becker, B., Gundelfinger, E. D. and Garner, C. C. (1995) Moleuclar characterization and spatial distribution of SAP97, a novel presynaptic protein homologous to SAP90 and the Drosophila dics-large tumor suppressor protein. J. Neurosci. 15, 2354-2366.
Müller, B. M. Kistner, U., Kindler, S., Chung, W. J., Kuhlendahl, S., Fenster, S. D., Lau, L. F., Veh, R. W., Huganir, R. L., Gundelfinger, E. D., Garner, C. C. (1996) Sap102, a novel postsynaptic protein that interacts with NMDA receptor complexes in vivo. Neuron. 17, 255-265.
Nguyen, T. and Sudhof, T. C. (1997) Binding properties of neuroligin 1 and neurexin 1 beta reveal function as heterophilic cell adhesion molecules. J. Biol. Chem. 272, 26032-26039.
Niethammer, M., Kim, E. and Sheng, M. (1996) Interaction between the C terminus of NMDA receptor subunits and multiple members of the PSD-95 family of membrane-associated guanylate kinases. J. Neurosci. 16, 2167
Nieto-Sampedro, M., Hoff, S. F. and Cotman, C. W. (1982) Perforated postsynaptic densities: probable intermediates in synapse turnover. Proc. Natl. Acad. Sci. USA. 79, 5718-5722.
Nusser, Z., Lujan, R., Laube, G., Roberts, J. D., Molnar, E., and Somogyi, P. (1998) Cell type and pathway dependence of synaptic AMPA receptor number and variability in the hippocampus. Neuron. 21, 545-559.
Nusser, Z., Mulvihill, E., Streit, P., and Somogyi, P. (1994) Subsynaptic segrgation of metabotropic and ionotropic glutamate receptors as revealed by immunogold localization. Neuroscience. 61, 421-427.
Olmsted, J. B. (1981) Affinity purification of antibodies from diazotized paper blots of heterogeneous protein samples. J. Biol. Chem. 256,11955-11957.
Ouimet, C. C., Mcguinness, T. L. and Greengard (1984) Immunocytochemical localization of calcium/calmodulin- dependent protein kinase II in rat brain. Proc. Natl. Acad. Sci. USA. 81, 5604-5608.
Pin, J. P. and Duvoisin, R. (1995) The metabotropic glutamate receptors: structure and functions. Neuropharmacology. 34, 1-26.
Pytela, R. and Wiche, G. (1980) High molecular weight polypeptides (270,000-340,000) grom cultured cells are related to hog brain microtubule-associated proteins but copurify with intermediate filaments. Proc. Nat. Acad. Sci. USA 77, 4808-4812.
Ratner, N. and Mahler, H. R. (1983) Structural organization of filamentous proteins in postsynaptic density. Biochem. 22, 2446-2453.
Rees, S., Guldner, F. H. and Aitkin, L. (1985) Activity dependent plasticity of postsynaptic density structure in the ventral cochlear nucleus of the rat. Brain Res. 325, 370-374.
Rho, A., Kim, E., Sheng, M. and Craig, M. (1998) Heterogeneity in the molecular composition of excitatory postsynaptic sites during development of hippocampal neurons in culture. J. Neurosci. 18, 1217-1229.
Sattler, R., Xiong, Z., Lu, W. Y., Hafner, M., MacDonald, J. F. and Tymianski, M. (1999) Specific coupling of NMDA receptor activation to nitric oxide neurotoxicity by PSD-95 protein. Science. 284, 1845-1848.
Schmidt, H. H. H. w., Pollock, J. S., Nakane, M., Gorsky, L. D., Forstermann, U. and Murad, F. (1991) Purification of a soluble isoform of guanylyl cyclase-activating-factor synthase. Proc. Natl. Acad. Sci. USA. 88, 3494-3498.
Schroer, T. A. (1994) Structure, function and regulation of cytoplasmic dynein. Curr. Opin. Cell. Biol. 6, 69-73.
Seubert, P., Baudry,M., Dudek, S. and Lynch, G. (1987) Calmodulin stimulates the degradation of brain spectrin by calpain. Synapse. 1, 20-24.
Sheng, M. and Pak, D. T. Ligand-gated ion channel interactions with cytoskeletal and signaling proteins. Annu. Rev. Physiol. 62, 755-778.
Shi, S., Hayashi, Y., Esteban, J. A., Malinow, R. (2001) Subunit-specific rules governing AMPA receptor trafficking to synapses in hippocampal pyramidal neurons. Cell. 105, 331-343.
Siekevitz, P. (1985) The postsynaptic density: a possible role in long-lasting effects in the central nervous system. Proc. Natl. Acad. Sci. U. S. A. 82, 3494-3498.
Siekevitz, P. (1985) The postsynaptic density: a possible role in long-lasting effects in the central nervous system. Proc. Natl. Acad. Sci. USA. 82, 3494-3498.
Smith, D. E., Fisher, P. A. (1984) Identification, developmental regulation, and response to heat shock of two antigenically relaged forms of a major nuclear envelope protein in Drosophila embryos: application of an improved method for affinity purification of antibodies using polypeptedes immobilized on nitrocellulose blots. J. Cell. Biol. 99, 20-28.
Sprengel, R., Suchanek, B., Amico, C., brusa, R., Burnashev, N., Rozov, A., Hvalby, O., Jensen, V., Paulsen, O., Andersen, P., Kim, J. J., Thompson, R. F., Sun, W, Webster, L. C., Grant, S. G. Eilers, J., Konnerth, A., Li, J., McNamara, J. O. and Seeburg, P. H. (1998) Importance of the intracellular domain of NR2 subunits for NMDA receptor function in vivo. Cell. 92, 279-289.
Srivastava, S., Osten, P., Vilim, F. S., Khatri, L., Inman, G., States, B., Daly, C., DeSouza, S., Abagyan, R., Valtschanoff, J. G. Weinberg, R. J. and Ziff, E. B. (1998) Novel anchorage of GluR2/3 to the postsynaptic density by the AMPA receptor-binding protein ABP. Neuron. 21, 581-591.
Strader, C. D., Pickel, V. M., Joh, T. H., Strohsacker, M. W., Shorr, R. G. L., Lefkowitz, R. J. and Caron, M. G. (1983) Antibodies to the b-adrenergic receptor: attenuation of catecholamine-sensitive adenylate cyclase and demonstration of postsynaptic receptor localization in brain. Proc. Natl. Acad. Sci. USA. 80, 1840-1844.
Suzuki, T. and Okumura, N. K. (1995) NMDA receptor subunits epsilon 1 (NR2A) and epsilon 2 (NR2B) are substrates for Fyn in the postsynaptic density fraction isolated from the rat brain. Biochem. Biophys. Res. Comm. 216,582-588.
Suzuki, T., Okumura, N. K., Tanaka, R., Ogura, A., Nakamura, K., Kudo, Y. and Tada, T. (1993) Characterization of protein kinase C activities in postsynaptic density fractions prepared from cerebral cortex, hippocampus and cerebellum. Brain Res. 619, 69-75.
Toni, N., Bucks, P. A., Nikonenko, I., Povilaitite, P., Parisi, L., and Muller, D. (2001) Remodeling of synaptic membranes after induction of long-term potentiation. J. Neurosci. 21, 6245-6251.
Vallee, R. (1993) Molecular analysis of the microtubule motor dynein.. Proc. Natl. Acad. Sci. U.S.A. 90, 8769-8772.
Walikonis, R. S., Jensen, O. N., Mann, M., Jr, D. W. P., Mercer, J. A. and Kennedy, M. B. (2000) Identification of proteins in the postsynaptic density fraction by mass spectrometry. J. Neurosci. 20, 4069-4080.
Walsh, M. J., and Kuruc, N. (1992) The postsynaptic density: constituent and associated proteins characterized by electrophoresis, immunoblotting, and peptide sequencing. J. Neurochem. 59, 667-678.
Walters, B. B. and Matus, A. I. (1975) Tubulin in postsynaptic junctional lattice. Nature. 347, 281-284.
Weber, K. and Osborn, M. (1969) The Reliability of Molecular Weight Determinations by Dodecyl Sulfate-Polyacrylamide Gel Electro- phoresis. J. Biol. Chem. 244, 4406-4412.
Westenbroek, R. E., Ahlijanian, M. K. and Catteral, W. A. (1990) Clustering of L-type Ca2+channel at the base of major densities in hippocampal pyramidal neurons. Nature. 347, 281-284.
Wiche, G., Becker, B., Luber, K., Weitzer, G., Castanon, M. J., Hauptmann, R., Stratowa, C., Stewart, M. (1991) Cloning and sequencing of rat plectin indicates a 466 kD polypeptide chain with a three-domain structure based on a central alpha-helical coiled coil. J. Cell. Biol. 114, 83-99.
Wu, K., Carlin, R., Sachs, L. and Siekebitz, P. (1985) Existence of a Ca2+-dependent K+ channel in synaptic membrane and postsynaptic density fractions isolated from canin cerebral cortex and cerebellum, as determined by apamn binding. Brain Res. 360, 183-194.
Wu, K., Nigam, S. K., Ledous, M., Huang, Y. Y., Aoki, C. and Siekevitz, P. (1992) Occurrence of the alpha subunits of G proteins in cerebral cortex synaptic membrane and postsynaptic density fractions: modulation of ADP-ribosylation by Ca2+/calmodulin. Proc. Natl. Acad. Sci. USA. 89, 8686-8690.
Wyszynski, M., Lin, J., Rao, A., Nigh, E., Beggs, A. H., Craig, A. M., and Sheng, M. (1997) Competitive binding of alpha-actinin and calmodulin to the NMDA receptor. Nature. 385, 439-442.
Xia, J., Zhang, X., Staudinger, J. and Huganir, R. L. Clustering of AMPA receptors by the synaptic PDZ domain-containing protein PICK-1. Neuron. 22, 179-187.
李碧芬 (1994) 豬腦中AMPA/kainate次類型麩胺酸受器之純化及特性分析,國立清華大學生命科學所碩士論文.
賴森林 (1998) 後突觸質密區結構之研究-蛋白質間雙硫鍵的形成與影響,國立清華大學生命科學所碩士論文.

QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top