臺灣博碩士論文加值系統

哺乳動物中樞神經中的突觸，為一種連接兩個神經細胞的特殊構造，後突觸質密區（postsynaptic density，簡稱PSD）位在突觸的末端，這群巨分子蛋白質群約包含30個左右的蛋白質，在立體空間中這些蛋白質有著嚴謹的排列及組成，使得PSD在突觸中扮演著重要調控的角色，因此，進一步瞭解PSD中的蛋白質組成，將有助於了解中樞神經中訊息傳遞過程。雖然如此，由於分析上的限制，PSD的蛋白質組成尚未完全被鑑定出來。我們實驗室利用3.5 % Weber & Osborn電泳膠片分析PSD中的蛋白質，於490±10 kDa發現一個蛋白質帶，命名為PSD500，該蛋白質占PSD蛋白質總量的3.5±0.3 %，由subcellular fraction及組織分布發現PSD500豐富存在於腦中的PSD，透過Concancavlin A-conjugated peroxidase的染色，PSD500可能是一個含有少量醣基的蛋白質，但以peptide N-glycosidase F（簡稱PNGF）去除醣基作用所造成PSD500分子量的改變並無法由3.5 % Weber & Osborn電泳膠片看出，因此PSD500上的醣基可能並非是N-linked glycans或此種分析系統無法觀察到少量分子量的差異。由MALDI-MS的結果，經由MS-Fit搜尋得到6個蛋白質名稱，其中兩個為b dynein heavy chain，四個為cytoplasmic dynein heavy chain，因此，我們初步認為PSD500可能為dynein heavy chain。

摘要
壹、緒論………………………………………………………………….1
貳、材料與方法…………………………………………………………10
(一) 藥品與方法…………………………………………………...10
(二) PSD的純化……………………………………………………11
(三) 凝膠電泳分析（SDS-PAGE）………………………………13
(四) 西方點漬法（Western Blot）………………………………...15
(五) 以Electroelution純化PSD-500……………………………...16
(六) DTE/IAA修飾所有雙硫基…………………………………...17
(七) 多株抗體（polyclonal antibody）製備………………………18
(八) 穩定度測定…………………………………………………...20
(九) 去醣基作用…………………………………………………...21
(十) Con A-conjugated peroxidase染色…………………………...21
(十一) In Solution Digest PSD500 for MALDI-MS……………….22
(十二) 氯仿－甲醇沈澱法………………………………………...23
(十三) 蛋白質濃度測定…………………………………………...23
(十四) 膠片染色法………………………………………………...24
參、結果
(一) PSD中的PSD500……………………………………………..25
(二) PSD500的分佈………………………………………………..26
(三) PSD500是否為一個醣蛋白…………………………………..27
(四) PSD500的穩定性……………………………………………..28
(五) 以MALDI-MS鑑定PSD500…………………………………28
肆、討論…………………………………………………………………30
伍、參考文獻…………………………………………………………...48
Appendix. I. The apparatus of Electroelution…………………………...58
Appendix. II. Useful Mass Spectrometry Web Site……………………..59
Appendix. III. Useful MALDI-MS fingerprint map web site ………….60
Appendix. IV. PSD500 are interior proteins within the PSD …………..61
Appendix. V. Scheme of KIFs and cytoplasmic dynein and their cargo organelles in nerve axons……………………………………………….62

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