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研究生:高千雯
研究生(外文):Kao Chien-wen
論文名稱:CDK4/6在紫外線所誘發的中國倉鼠卵巢細胞自殘現象中扮演的角色
論文名稱(外文):The role of cdk4/6 in UVC-induced apoptosis of CHO-k1 cells
指導教授:劉銀樟
指導教授(外文):Yin-chang Liu
學位類別:碩士
校院名稱:國立清華大學
系所名稱:生命科學系
學門:生命科學學門
學類:生物學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:英文
論文頁數:45
中文關鍵詞:紫外線誘發CDK4/6細胞自殘p16基因
外文關鍵詞:UVCCDK4/6apoptosisp16
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根據之前的研究,中國倉鼠卵巢細胞中無法表現p21的mRNA及蛋白質。此外,在接受25 J/m2的UV光照射後,中國倉鼠卵巢細胞會有大量死亡的現象產生。而當人類p21基因送入細胞中大量表現後,這種現象便會被抑制下來。p21蛋白質的功用可以抑制一般cdk或cdk2, 4, 6等。為了探討出是哪一個cdk在這個由UVC所引發的大量死亡現象中扮演了重要的角色,我們建構了一個mouse p16的表現質體,送入中國倉鼠卵巢細胞中表現,看是否能阻止這種由UVC所引起的,大量死亡的現象。p16能抑制cdk4,6 的活性。透過在中國倉鼠卵巢細胞中大量表現p16,我們便可知道是cdk2或4/6在這個路徑中扮演重要的角色。經由流式細胞儀的分析結果,我們發現在中國倉鼠卵巢細胞中大量表現p16並不能抑制住其大量死亡的現象。同時,cdk4的活性分析實驗結果,顯示cdk4的活性不因照射UV而變化。因此,cdk4/6在中國倉鼠卵巢細胞受UVC照射後大量死亡的現象中,可能不是關鍵點。
It was found previously that the expression of p21 mRNA and protein is deficient in CHO-K1 cells. And also, after 25 J/m2 of UV irradiation, significant death of CHO-K1 cells appears. And after overexpression of human p21 gene in CHO-K1 cells, this UV induced apoptosis can be reduced. p21 acts as a general cdk inhibitor. In order to know which cdk is involved in this UVC induced apoptosis, we constructed a p16 expression plasmid, and transfected it into CHO-K1 cells to see if the expression of p16 protein in CHO-K1 cells can reduce the UV-induced apoptosis. p16 is a inhibitor of cdk 4, 6 . From the flow cytometry result, we found that overexpression of p16 in CHO-K1 cells was unable to inhibit the UV-induced apoptosis. Moreover, the cdk4 kinase activity remained unchanged following UV irradiation. Thus, cdk 4, 6 play little or no roles in UVC induced apoptosis in CHO-K1 cells.
Abstracts (English)………………………………………….. 1
Abstracts (Chinese)…………………………………………. 2
Introduction…………………………………………………..3
Materials and methods……………………………………….7
Results……………………………………………………….16
Discussion…………………………………………………...22
References…………………………………………………...27
Figures and legends………………………………………….30
Appendix…………………………………………………….41
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