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研究生:葉仲書
研究生(外文):Chung-Shu Yeh
論文名稱:秀姑巒溪季節性上溯鱸鰻之族群遺傳結構
論文名稱(外文):Genetic Structure of the Seasonal Upstream Migration Populations of Anguilla marmorata in Hsiukuluan River
指導教授:曾晴賢曾晴賢引用關係
指導教授(外文):Chyng-Shyan Tzeng
學位類別:碩士
校院名稱:國立清華大學
系所名稱:生命科學系
學門:生命科學學門
學類:生物學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:中文
論文頁數:47
中文關鍵詞:鱸鰻產卵場族群遺傳結構粒線體控制區
外文關鍵詞:Anguilla marmorataSpawning areaGenetic population structureD-loop
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鱸鰻Anguilla marmorata Quoy & Gaimard 1924為廣泛分布之熱帶型鰻,在印度洋沿岸及西太平洋的各大島嶼上均可發現其蹤跡。先前在秀姑巒溪的研究指出台灣東部的鱸鰻鰻苗每年有兩個主要的來游期,且在夏季有上溯高峰群,而從耳石日齡的分析也證實鱸鰻每年有兩個主要的產卵高峰。由於不同年間的來游模式相當固定,本研究嘗試以分子生物技術驗證生殖時間的隔離是否導致不同來游時期的鰻苗發生族群分化。
本研究蒐集了自1995年11月至2001年5月間在秀姑巒溪採集的鱸鰻鰻苗共128尾,以粒線體控制區前端566鹼基對的變異建構親緣關係樹及進行多元尺度法分析,同時比較不同時間來游族群間的遺傳分化指標 (Nst) 和基因互流量 (Nm) 。所有結果皆指出不同生殖族群之間沒有明顯的差異存在,生殖季的不同並未造成族群的分化。
然而不論親緣關係樹或多元尺度法的結果都指出北太平洋的鱸鰻可能不是單一生殖族群。配合洋流資料及分子遺傳學的結果,本研究認為北太平洋可能有數個不同的鱸鰻族群,且不同的族群有其各自的產卵場。
Anguilla marmorata Quoy & Gaimard 1924 is widespread in the fresh waters of the islands and the coast of the Indo-Pacific Ocean. It was found that there are two major upstream migrations of the eel and reaches a peak during the summer time. The otolith data also indicate that there are two major spawning seasons in a year (summer and winter). For the constant recruitment patterns, we would like to know whether the separation of spawning seasons results in the differentiation of A. marmorata populations.
In this study, 128 migratory elvers were collected from November 1995 to May 2001 at Hsiukuluan River in Eastern coast of Taiwan. To reveal the genetic population structure, neighbor-joining tree and multidimensional scaling (MDS) analysis were done from the first 566 bp of the mitochondrial control region, and the coefficient of nucleotide differentiation (Nst) and gene flow (Nm) were estimated. No significant difference were found between different recruitment populations, indicated that the separation of spawning seasons did not lead to the division of A. marmorata populations.
However, results of both neighbor-joining tree and MDS analysis indicated that A. marmorata in the North Pacific Ocean may not be a panmictic population. Considering both current and genetic data, we believe that there may be several subpopulations of A. marmorata in the North Pacific region, and furthermore, each subpopulation has its own spawning area.
中文摘要………………………………………………………………Ⅰ
Abstract………………………………………………………………Ⅱ
謝誌……………………………………………………………………Ⅲ
目錄……………………………………………………………………Ⅳ
圖表目錄………………………………………………………………Ⅴ
壹、前言
一、鰻鱺科分類地位與分布………………………………………1
二、鰻鱺科生態習性簡介…………………………………………1
三、鱸鰻生態習性簡介……………………………………………2
四、粒線體控制區的特性…………………………………………4
五、研究動機與目的………………………………………………5
貳、材料及方法
一、樣本採集………………………………………………………6
二、實驗方法………………………………………………………6
三、資料分析………………………………………………………9
參、結果
一、採集結果………………………………………………………12
二、定序結果分析…………………………………………………12
三、親緣關係之重建………………………………………………13
四、族群內遺傳組成多型性分析…………………………………14
五、族群間分化程度分析…………………………………………19
肆、討論
一、親緣關係之重建………………………………………………21
二、生殖季之隔離與族群分化……………………………………22
三、北太平洋鱸鰻之族群結構……………………………………23
伍、結論………………………………………………………………28
陸、引用文獻…………………………………………………………29
圖………………………………………………………………………34
表………………………………………………………………………43
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