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研究生:林正雄
論文名稱:大腦皮質神經元glutamatereceptor的發育表現變化及動作電位的研究
指導教授:張兗君
學位類別:碩士
校院名稱:國立清華大學
系所名稱:生命科學系
學門:生命科學學門
學類:生物學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:中文
論文頁數:59
中文關鍵詞:動作電位麩胺酸
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摘要
神經突觸(synapse)是神經細胞之間訊號傳遞的主要橋樑,經由動作電位所造成的去極化使Ca2+進入前突觸而釋放神經傳導物質,之後由位於後突觸的受器接收反應。在哺乳類的中樞神經系統中,麩胺酸(glutamate)是一重要的興奮性神經傳導物質,其在神經興奮路徑中的傳達受器主要是NMDA receptor以及AMPA receptor。然而在神經細胞發育的初期,此兩種受器在突觸上的表現過程尚未清楚。在本研究中以取自發育18天胎鼠(E18)的大腦皮質神經細胞加以培養作為模型系統,在不同培養時期,以電生理技術記錄由麩胺酸所引發的電流,並加以分析,希望明瞭該兩類受器的發生過程。結果發現在培養了一週(7DIV或8DIV)左右的E18神經細胞,所測得流入細胞暫態電流,主要是經由AMPA receptor,但也可觀察到有NMDA receptor共同參與。E18並不是使所取的神經細胞表現出NMDA receptor的關鍵所在,我們培養E17、E16的胎鼠大腦皮質神經細胞在7DIV也可觀察到NMDA receptor所引發的電流。而在E18的系統,我們可觀察到NMDA receptor表現的最早時間是在4DIV,顯示E18,7DIV的胎鼠大腦皮層神經細胞主要是AMPA receptor的表現,但NMDA receptor也已進入突觸而開始有所功能。
另外我們也觀察到有些2、3DIV的神經細胞雖然只有少量的sEPSCs(Spontaneous EPSCs),但已有頻繁的動作電位的發生,而此時的動作電位並非如7DIV之後的細胞,僅是由Glutamate receptor所引發,因為AMPA receptor的擷抗劑並無抑制作用。以上初步的結果,意味神經細胞發育過程中,神經訊息傳遞的方式可能有所變更,深入研究此一趣味十足的現象,將有助於了解神經系統的發育的過程。

目錄
壹 摘要…………………1
貳 緒論…………………2
參 材料與方法…………8
肆 結果…………………14
伍 討論…………………20
陸 參考文獻……………27
柒 圖……………………31

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