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研究生:吳健鉦
研究生(外文):C. C. Wu
論文名稱:使用粒徑分析儀測定耐輻射奇異球菌菌株之細胞體積分佈:分佈曲線中明顯高峰之含意
論文名稱(外文):Determination of Cell-szie Distribution of a Deinococcus radiodurans Culture: Implication of Distinguishable Peaks in the Distribution Curve
指導教授:譚世特
指導教授(外文):Shieh-Te Tan
學位類別:碩士
校院名稱:國立清華大學
系所名稱:生命科學系
學門:生命科學學門
學類:生物學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:中文
論文頁數:46
中文關鍵詞:耐輻射奇異球菌細胞體積
外文關鍵詞:Deinococcus rasioduranscell size
相關次數:
  • 被引用被引用:0
  • 點閱點閱:181
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本研究使用高解析力粒徑分析儀測定耐輻射奇異球菌菌液中細胞體積並以分佈曲線表示之。這些體積分佈曲線經過特定的數據處理後可得到許多明顯的高峰。體積高峰的出現提供了數字化的細胞體積分佈狀態,因而易於觀察比較樣本之間的差異。例如,以上述方法比較耐輻射奇異球菌野生型菌株與生長速度較慢的突變株的不同,發現突變株生長緩慢似乎與其具有顯著較長的 D 期(相當於真核細胞的 G2/M 期)有關。此較長的 D 期反映在較多數量的體積高峰上。
這樣的初步結果暗示,體積分佈曲線中體積高峰反映奇異球菌細胞生長時體積的增大是受到嚴格控制的。為進一步瞭解此種體積增大控制現象的本質,我們對二十八個耐輻射奇異球菌培養菌液進行觀測,進行數據處理後,得到高出現頻率體積(FOVs, frequently occurring volumes)的出現頻率圖。體積峰出現頻率呈現兩個出現頻率較高的體積區間。配合細胞族群的體積分佈狀態,與細胞週期的概念,可以概略的分辨細胞週期對應的體積峰。改變生長條件對於細胞的影響,也可以由體積峰的變化輕易的觀察之。

Abstract
Sizes of individual cells in a Deinococcus radiodurans IR culture were measured by a Coulter size analyzer, and the resulted cell-particle-size distribution curves was found to display obvious peaks. A data modification system was used to render these peaks more distinguishable but at the same time causing no alternations in the basic traits of the distribution curve. The distinguishable peaks defined volumes as numeric (quantificational) data, thus help comparison study. For example, by using this size-distribution-data-processing approach, we were able to conclude that in comparison with its wild-type strain (IR), a slow-growing mutant strain (X2) was defective in the cell separation stages producing a much longer D phase. The results suggest that cell size increase in D. radiodurans was in tight control. Twenty-eight D. radiodurans IR cultures were similarly assayed and data modification system was used. Pooled results showed frequently occurring volumes (FOVs). Some FOVs constitute high-frequency regions in the cell size distribution curves. Based in the results, the pattern of the distinguishable peaks appears to be useful in studying cell growth physiology. The defined FOVs are suggestive of a tight cell-size control during the growth of a bacterial cell. In summary, cell-size increase during bacterial growth appears to be discontinuous in a stepwise fashion.

1. 中文摘要.....................................................-1-
2. 英文摘要.....................................................-2-
3. 簡介.........................................................-3-
4. 材料與方法...................................................-5-
5. 結果........................................................-10-
6. 討論........................................................-18-
7. 參考文獻....................................................-23-
8. 圖表..........................................................i

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