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研究生:鄧伊珊
研究生(外文):Yi-Shan Teng
論文名稱:胎鼠大腦皮質神經元突觸生成之研究
指導教授:張兗君
指導教授(外文):Yen-Chung Chang
學位類別:碩士
校院名稱:國立清華大學
系所名稱:生物技術研究所
學門:生命科學學門
學類:生物科技學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:中文
論文頁數:91
中文關鍵詞:大腦皮質神經元突觸生成
外文關鍵詞:cortexsynaptpgensis
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神經細胞藉由突觸的連結而相互聯繫,因此突觸被視為訊息傳導的基本單位。 突觸在發育時,後突觸膜上會出現神經傳導受器的聚集,故了解突觸如何調控後突觸膜上受器的聚集,為了解神經細胞發育的重要課題。
我們發現於相同神經細胞密度下,將細胞以無血清培養 (serum-free medium) 與含血清培養 (MEM) 相比較,在培養第一週所紀錄到EPSCs訊號的頻率落在相同範圍內,但其大小則以無血清培養者較大;並且隨著細胞培養密度的提高,可紀錄到NMDAR mediated EPSCs的機率也增加。 隨著培養天數 (第二週及第三週),EPSCs頻率和大小都有增加的趨勢,但差異不明顯。 另外輔以螢光染色方法,將電生理紀錄下注入Lucifer-Yellow的細胞染synaptophysin;發現神經細胞培養早期 (immature),約有近六成的synaptophysin clusters位於突觸外(extra-synaptic) ,隨發育天數約有九成以上的synaptophysin clusters位於突觸 (synaptic) 位置上。 因此,亦針對培養早期 (2 or 3 DIV) 之細胞,microfilament、tubulin、AMPA receptors、 PSD-95 以及synaptophysin等進行螢光染色,期盼對早期突觸之生成有進一步的認識。

摘要
壹、緒論
貳、材料與方法
參、結果
肆、討論
伍、參考文獻
陸、圖表集
一、圖
二、表
柒、附錄

Allison, D. W., Gelfand, V. I., Spector, I., and Craig, A. M. (1998). Role of actin in anchoring postsynaptic receptors in cultured hippocampal neurons : differential attachment of NMDA versus AMPA receptors. The Journal of Neuroscience, 18(7): 2423-2436.
Basarsky, T. A., Parpura, V., and Haydon, P. G. (1994). Hippocampal synaptogenesis in cell culture : developmental time course of synapse formation, calcium influx, and synaptic protein distribution. Journal of Neuroscience, 14: 6402-6411.
Behar, T. A., Scott, C. A., Greene, C. L., Wen, X., Smith, S. V., Maric, D., Liu, Q. Y., Colton, C. A., and Barker, J. L. (1999). Glutamate acting at NMDA receptors stimulates embryonic cortical neuronal migration. Journal of Neuroscience, 19(11): 4449-4461.
Brewer, G. J. (1997). Isolation and culture of adult rat hippocampal neurons. Journal of Neuroscience Methods, 71: 143-155.
Bradke, F., and Dotti, C. G. (2000). Establishment of neuronal polarity : lessons from cultured hippocompal neurons. Current Opinion in Neurobiology, 10: 574-581.
Cady, C., Evans, M. S., and Brewer, G. J. (2001). Age-related differences in NMDA responses in cultured rat hippocampal neurons. Brain Research, 921: 1—11.
Cattabeni, F., Gardoni, F., and Luca, M. D. (1999). Pathophysiological implications of the structural organization of the excitatory synapse. European Journal of Pharmacology, 375: 339-347.
Chen, G., Trombley, P. Q., and Van Den Pol A. N. (1995). GABA receptors precede glutamate receptors in hypothalamic development;differential regulation by astrocytes. Journal of Neurophysiology, 74(4): 1473-1484.
Cline, H. T. (2001). Dendritic arbor development and synaptogenesis. Current Opinion in Neurobiology, 11: 118-126.
Constantine-Paton, M., and Cline, H. T. (1998). LTP and activity- dependent synaptogenesis : the more alike they are, the more different they become. Current Opinion in Neurobiology, 8: 139-148.
Cottrell, J. R., Dube, G. R., Egles, C., and Liu, G. (2000). Distribution, density, and clustering of functional glutamate receptors before and after synaptogenesis in hippocampal neurons. Journal of Neurophysiology, 84: 1573-1587.
Craig, A. M. (1998). Activity and synaptic receptor targeting:the long view. Neuron, 21: 459-462.
De Lima, A. D., Merten, M. D. P., and Voigt, T. (1997). Neuritic differentiation and synaptogenesis in serum-free neuronal cultures of the rat cerebral cortex. Journal of Comparative Neurology, 382: 230-246.
Dickson, B. J., and Senti, K. A. (2002). Axon guidance: growth cones make an unexpected turn. Current Biology, 12: 218-220.
Dotti, C. G., Sullivan, C. A., and Banker, G. A. (1988). The establishment of polarity by hippocampal neurons in culture. The Journal of Neuroscience, 8(4): 1454-1468.
Dumoulin, A., Levi, S., Riveau, B., Gasnier, B., and Triller, A. (2000). Formation of mixed glycine and GABAergic synapses in cultured spinal cord neurons. European Journal of Neuroscience, 12: 3883-3892.
El-Husseini, A. E., Schnell, E., Chetkovich, D. M., Nicoll, R. A., and Bredt, D. S. (2000). PSD- 95 involvement in maturation of excitatory synapses. Science, 290(17): 1364-1368.
Ehlers, M. D., Mammen, A. L., Lau, L. F., and Huganir, R. L. (1996). Synaptic targeting of glutamate receptors. Current Opinion in Neurobiology, 8: 484-489.
Evans, M. S., Collings, M. A., and Brewer, G. J. (1998). Electro- physiology of embryonic, adult and aged rat hippocampal neurons in serum-free culture. Journal of Neuroscience Methods, 79: 37-46.
Feldman, D. E., and Knudsen, E. I. (1998). Experience-dependent plasticity and the maturation of glutamatergic synapses. Neuron, 20: 1067-1071.
Feller, M. B. (1999). Spontaneous correlated activity in developing neural circuits. Neuron, 22: 653-656.
Fletcher, T. C., Cameron, P., Camilli, P. D., and Banker, G. (1991). The distribution of synapsinⅠ and synaptophysin in hippocampal neurons developing in culture. The Journal of Neuroscience, 11(6): 1617-1626.
Fletcher, T. C., Camilli, P. D., and Banker, G. (1994). Synaptogenesis in hippocampal cultures : evidence indicating that axons and dendrites become competent to from synapses at different stages of neuronal development. Journal of Neuroscience, 14: 6695-6706.
Friedman, H. V., Bresler, T., Garner, C. C., and Ziv, N. E. (2000). Assembly of new individual excitatory synapses: time course and temporal order of synaptic molecule recruitment. Neuron, 27(1):57-69
Gao, X. B., Chen, G., and Van Den Pol A. N. (1998). GABA- dependent firing of glutamate-evoked action potentials at AMPA / Kainate receptors in developing hypothalamic neurons. Journal of Neurophysiology, 79: 716-728.
Gao, X. B., and Van Den Pol A. N. (2000). GABA, not glutamate, a primary transmitter driving action potentials in developing hypothalamic neurons. Journal of Neurophysiology, 85: 425-434.
Garner, C. C., Zhai, R. G., Gundelfinger, E. D., and Ziv, N. E. (2002). Molecular mechanisms of CNS synaptogenesis. TRENDS in Neuroscience, 25(5): 243-250.
Goldin, M., Segal, M., and Avignone, E. (2001). Functional plasticity triggers formation and pruning of dendritic spines in cultured hippocampal networks. Journal of Neuroscience, 21, 186-193.
Gomperts, S. N. (1996). Clustering membrane proteins : it’s all coming together with the PSD-95/ SAP90 protein family. Cell, 84, 659-662.
Goodhill, G. J. (1998). Mathematical guidance for axons. Trends Neuroscience, 21: 226-231.
Halpain, Shelley. (2000). Actin and the agile spine : how and why do dendritic spines dance ? TRENDS in Neuroscience, 23(4): 141-146.
Harris, K. M. (1999). Structure, development, and plasticity of dendritic spines. Current Opinion in Neurobiology, 9: 343-348.
Hori, Y., and Kanda, K. (1996). Developmental alterations in NMDA receptor-mediated currents in neonatal rat spinal motoneurons. Neuroscience Letters, 205: 99-102.
Huntley, G. W., Benson, D. L., and Colman, D. R. (2002). Structural remodeling of the synapse in response to physiological activity. Cell, 108: 1-4.
Husi, H., Ward, M. A., Choudhary, J. S., Blackstock, W. P., and Grant, Seth G. N. (2000). Proteomic analysis of NMDA receptor- adhesion protein signaling complexes. Nature Neuroscience, 3(7): 661-669.
Inglis, F. M., Zuckerman, K. M., and Kalb, R. G. (2000). Experience-dependent development of spinal motor neurons. Neuron, 26: 299-305.
Jahn, R., and Schuman, E. M. (2001). Neuronal and glial cell biology. Current Opinion in Neurobiology, 11: 533-535.
Jontes, J. D. , and Smith, S. J. (2000). Filopodia, spines, and the generation of synaptic diversity. Neuron, 27: 11-14.
Kennedy, M. B. (1997). The postsynaptic density at glutamatergic synapses. TRENDS in Neuroscience, 20: 264-268.
Klintsova, A. Y., and Greenough, W. T. (1999). Synaptic plasticity in cortical systems. Current Opinion in Neurobiology, 9: 203-208.
Kornau, H. C., Schenker, L. T., Kennedy, M. B., and Seeburg, P. H. (1995). Domain interaction between NMDA receptor subunits and postsynaptic density protein PSD-95. Science, 269: 1737- 1740.
Kovacs, A. D., Cebers, G., Cebere, A., Moreira, T., and Liljequist, S. (2001). Cortical and striatal neuronal cultures of the same embryonic origin show intrinsic differences in glutamate receptor expression and vulnerability to excitotoxicity. Experimental Neurology, 168: 47-62.
Lee, S. H., and Sheng, M. (2000). Development of neuron-neuron synapses. Current Opinion in Neurobiology, 10: 125-131.
Lesuisse, C., and Martin, L. J. (2002). Long-term culture of mouse cortical neurons as a model for neuronal development, aging, and death. J. Neurobiol. , 51(1): 9-23.
Li, Y. X., Schaffner, A. E., and Barker, J. L. (1999). Astrocytes regulate the developmental appearance of GABAergic and glutamatergic postsynaptic currents in cultured embryonic rat spinal neurons. European Journal of Neuroscience, 11: 2537-2551.
Liao, D., Scannevin, R. H.,and Huganir, R. (2001). Activation of silent synapses by rapid activity-dependent synaptic recruitment of AMPA receptors. Journal of Neuroscience, 21(16): 6008-6017.
Lin, Y. C., Huang, Z. H., Jan, I. S., Yeh, C. C., Wu, H. J., Chou, Y. C., and Chang, Y. C. (2002). Development of excitatory synapses in cultured neurons dissociation from the cortices of rat embryos and rat pups at birth. Journal of Neuroscience Research, 67: 484-493.
Milner, L. D., and Landmesser, L. T. (1999). Cholinergic and GABAergic inputs drive patterned spontaneous motoneuron activity before target connact. Journal of Neuroscience, 19: 3007-3022.
Monuki, E. S., and Walsh, C. A. (2001). Mechanisms of cerebral cortical patterning in mice and humans. Nature neuroscience, 4: 1199-1206.
Morrow, T., Song, M. R., and Ghosh, A. (2001). Sequential specification of neurons and glia by developmentally regulated extracellular factors. Development, 128: 3585-3594.
Mozhayeva, M. G., Sara, Y., Liu, and Kavalali, E. T. (2002). Development of vesicle pools during maturation of hippocampal synapses. The Journal of Neuroscience, 22(3): 654-665.
Nusser, Z. (2000). AMPA and NMDA receptors : similarities and differences in their synaptic distribution. Current Opinion in Neurobiology, 10: 337-341.
O’Brien, R. J., Mammen, A. L., Blackshaw, S., Ehlers, M. D., Rothstein, J. D., and Huganir, R. L. (1997). The development of excitatory synapses in cultured spinal neurons. The Journal of Neuroscience, 17(19): 7339-7350.
O’Brien, R. J., Lau, Lit-Fui, and Huganir, R. L. (1998). Molecular mechanisms of glutamate receptor clustering at excitatory synapses. Current Opinion in Neurobiology, 8: 364-369.
O’Brien, R. J., Kamboj, S., Ehlers, M. D., Rosen, K. R., Fischbach, G. D., and Huganir, R. L. (1998). Activity-dependent modulation of synaptic AMPA receptor accumulation. Neuron, 21: 1067-1078.
Ooyen, A. V., Willshaw, D. J., and Ramarkers, Ger J. A. (2000). Influence of dendritic morphology on axonal competition. Neurocomputing, 32-33: 255-260.
Petralia, R. S., Esteban, J. A., Wang, Y-X., Partridge, J. G., Zhao, H-M., and Wenthold, R. J. (1999). Selective acquisition of AMPA receptors over postnatal development suggests a molecular basis for silent synapses. Nature Neuroscience, 2(1): 31-36.
Pfrieger, F. W., and Barres, B. A. (1997). Synaptic efficacy enhanced by glial cells in vitro. Science, 277(12): 1684-1687.
Potier, B., Poindessous-Jazat, F., Dutar, P., and Billard, J. M. (2000). NMDA receptor activation in the aged rat hippocampus. Experimental Gerontology, 35: 1185-1199.
Rao, A., Kim, E., Sheng, M., and Craig, A. M. (1998). Heterogeneity in the molecular composition of excitatory postsynaptic sites during development of hippocampal neurons in culture. The Journal of Neuroscience, 18(4): 1217-1229.
Renger, J. J., Egles, C., and Liu, G. (2001). A developmental switch in neurotransmitter flux enhances synaptic efficacy by affecting AMPA receptor activation. Neuron, 29: 469-484.
Ritzerthaler, S., Suzuki, E., and Chiba, A. (2000). Postsynaptic filopodia in muscle cells interact with innervating motoneuron axons. Nature Neuroscience, 3: 1012-1017.
Robert, A., Howe, J. R., and Waxman, S. G. (2000). Development of glutmatergic synaptic activity in cultured spinal neurons. Journal of Neurophysiology, 83(2): 659-670.
Ruthel, G., and Hollenbeck, P. J. (2000). Growth cones are not required for initial establishment of polarity or differential axon branch growth in cultured hippocampal neurons. The Journal of Neuroscience, 20(6): 2266-2274.
Scheetz, A. J., Nairn, A. C., and Constantine-Paton, M. C. (2000). NMDA receptor-mediated control of protein synthesis at developing synapses. Nature neuroscience, 3(3): 211-216.
Ullian, E. M., Sapperstein, S. K., Christopherson, K. S., and Barres, B. A. (2001). Control of synapse number by glia. Science, 291(26): 657-660.
Wiedenmann, B., and Franke, W. W. (1985). Identification and localization of synaptophysin , an integral membrane glycoprotein of Mr 38,000 characteristic of presynaptic vesicles. Cell, 41: 1017- 1028.
Xiao, M. Y., Niu, Y. P., Dozmorov, M., and Wigstrom, Holger. (2001). Comparing fluctations of synaptic responses mediated via AMPA and NMDA receptor channels — implications for synaptic plasticity. BioSystem, 62: 45-56.
Xie, C., Markesbery, W. R., and Lovell, M. A. (2000). Survival of hippocampal and cortical neurons in a mixture of MEM+ and B27-supplemented neurobasal medium. Free Radical Biology & Medicine, 28(5): 665-672.
Ziv, N. Z., and Garner, C. C. (2001). Principles of glutamatergic synapse formation : seeing the forest for the tree. Current Opinion in Neurobiology, 11: 536-543.
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消長之關聯性研究。 國立清華大學生命科學研究所碩士論文
吳漢傑 (1999) 鼠大腦神經元發育早期樹突小刺型態與生化之研究。 國立清華大學生命科學研究所碩士論文
孫志彰 (2000) 細胞內鋅離子對鼠大腦皮質神經元麩胺酸受器之調
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鄭宜珊 (2001) 胎鼠大腦神經元發育早期之麩胺酸受器次級單位轉
變。 國立清華大學生命科學研究所碩士論文
林彥昌 (2001) 鼠大腦神經元發育早期興奮性麩胺酸受器之研究。
國立清華大學生命科學研究所碩士論文

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