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研究生:張宇清
研究生(外文):Yu-Ching Chang
論文名稱:紫外線誘發增生細胞核抗原表現之意涵
論文名稱(外文):The implication of proliferating cell nuclear antigen gene induction by UV irradiation
指導教授:劉銀樟
指導教授(外文):Yin-Chang Liu
學位類別:碩士
校院名稱:國立清華大學
系所名稱:生物技術研究所
學門:生命科學學門
學類:生物科技學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:英文
論文頁數:48
中文關鍵詞:增生細胞核抗原紫外線氧化自由基
外文關鍵詞:Proliferating cell nuclear antigenUVReactive oxygen species
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增生細胞核抗原是一種DNA聚合酵素δ與ε的輔助因子,也是真核細胞DNA合成與修補所需。在不同劑量的紫外線照射下,哺乳類動物的增生細胞核抗呈現不同程度的表現。而在實驗室之研究中,發現在人類細胞的增生細胞核抗原基因表現的紫外線反應與p53的存在與否無關。在本論文中,我們發現因紫外線照射所引發的增生細胞核抗原和p53下游基因p21WAF1和GADD45表現是有顯著不同。因紫外線而引發的增生細胞核抗原或是熱休克蛋白質70的表現時間會晚於c-Jun而早於p21WAF1及GADD45。而這種因紫外線所引起的增生細胞核抗原基因表現與DNA的修補並沒有關係;因為在具有DNA修補機制缺失之UVL-10及xrs-6細胞中也可以看到如上所述之現象。此外,雖然紫外線的照射不會增加細胞內氧化自由的含量,但是當利用抗氧化劑N-acetyl-cysteine 及glutathione,卻可以抑制因紫外線所引起的增生細胞核抗原基因表現。

Proliferating cell nuclear antigen (PCNA), also known as an auxiliary factor of DNA polymerase δand ε, is required in both DNA synthesis and DNA excision repair in eukaryotes. The gene expression of proliferating cell nuclear antigen (PCNA) in mammalian cells was moderately induced by UV irradiation (at 254 nm) at dose dependent manner. In the previously study, we found that p53 is dispensable in UV induction of PCNA expression. In this study, we observed that PCNA gene expression was induced by UV irradiation in a kinetic pattern, which was different from those of p53 inducible gene such as p21WAF1 or GADD45. The UV induction of PCNA or HSP70 was few hours behind that of c-Jun, but few hours before those of p21WAF1 and GADD45. Furthermore, the UV -induced PCNA expression did not depend on the DNA repair since PCNA was UV inducible in UVL-10 and xrs-6 cells, where the nucleotide excision repair and the double stranded repair is largely compromised, respectively. Moreover, despite the fact that no change of reactive oxygen species was observed in UVC-irradiated cells, the induction of PCNA expression was inhibited by antioxidants N-acetyl-L-cysteine and glutathione.

1. English abstract--------------------------------1
2. Chinese abstract--------------------------------2
3. Introduction------------------------------------3
4. Materials and Methods---------------------------8
5. Results----------------------------------------13
6. Discussion-------------------------------------16
7. Reference--------------------------------------19
8. Figure legends---------------------------------29

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