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研究生:林立心
研究生(外文):LiHsin Lin
論文名稱:碳六十固定化酵素電化學葡萄糖感測器研製與應用
論文名稱(外文):Preparation and Application of Immobilized Fullerence-Enzyme Based electrochemical glucose Sensor
指導教授:施正雄施正雄引用關係
指導教授(外文):Jeng Shong Shih
學位類別:碩士
校院名稱:國立臺灣師範大學
系所名稱:化學研究所
學門:自然科學學門
學類:化學學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:中文
論文頁數:115
中文關鍵詞:葡萄糖碳六十固定化酵素
外文關鍵詞:glucoseImmobilized Fullerence-Enzyme
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本研究利用具混價性質之鈷赤血鹽和碳六十固定化酵素致被化學修飾電極來發展葡萄糖感測器,本實驗在0.1MKCl,pH6之磷酸鹽緩衝液中,施加電位0.0V(相對於Ag/AgCl參比電極)偵測葡萄糖,藉由含鈷赤血鹽對H2O2之催化能力,可將偵測電位降低至0.0V,且在還原模式下進行,此電位有效防止血液中電活性物質,如維生素C(Ascorbic acid),尿酸(Uric acid),乙醯基酚(Acetaminophen),半胱銨酸(Cysteine),酪銨酸(Tyrosine),半乳糖(Galactose)之干擾.H2O2氧化還原態的鈷赤血鹽而自身被還原,此還原電流和溶液中的葡萄糖濃度成正比關係.此碳六十固定化酵素感測器對葡萄糖具有不錯之線性範圍(8mM),快速的反應時間(5秒),以及不錯之靈敏度,其偵測極限可達1.6×10-6M,在20次連續操作下相對標準偏差維4.26%,使用100天後,訊號仍維持最初之80%,實驗顯示碳六十固定化酵素確實能增加訊號和延長感測器壽命.本研究也對此葡萄糖感測器探討了溫度,酸鹼度等效應.

A mixed-valence cluster of cobalt(Ⅱ)hexacyanoferrate and fullerene-enzyme based electrochemical glucose sensor was developed.A water insoluble fullerene-glucose oxidase(C60-GOD)was synthesized and applied as an immobilized enzyme on a glassy carbon electrode with cobalt(Ⅱ)hexacyanoferrate for analysis of glucose.The glucose was measured in 0.1MKCl/phosphate buffer solution with a pH of 6.0 When the electrode potential was applied at 0.0mV(v.s Ag/AgCl reference electrode).The glucose sensor exhobited efficient electro-catalytic activity toward the liberated hydrogen peroxide and allowed cathodic detection of the glucose,with no interferences from easily oxidizable constitutes such as uric acid,ascorbic acid,cysteine,tyrosine,acetaminophen and glactose.The H2O2 oxidized the reduced cobalt(Ⅱ)hexacyanoferrate and was reduced itself,and the reduced current was proportional to the concentration of glucose in aqueous solutions.The immobilized C60-GOD based glucose sensor showed a good linear response up to 8mM and a quite short response time of 5 sec.The glucose sensor also exhibited good sensitivity with a detection limit of 1.6×10-6M and a high reproducibility with a relative standard deviation(RSD) of 4.26%.The signal decreased to 80% of the initial value after continuous operations for 100 days.The immobilized Fullerene/glucose oxidase obiously enhanced the response singal and extends the lifetime of the glucose enzyme sensor.Effects of pH and temperature on the responses of the immobilized C60-GOD based glucose sensor were also studied and discussed.

目錄
中文摘要 Ⅰ
英文摘要 Ⅱ
目錄 Ⅲ
圖目錄 Ⅵ
表目錄 Ⅷ
第一章 緒論 1
1-1生化感測器 1
1-1-1生化感測器之定義 1
1-1-2辨識元之種類 2
1-1-3辦識元之固定法 4
1-2葡萄糖氧化酵素的介紹 10
1-2-1酵素化學 10
1-2-2酵素的發現史 10
1-2-3酵素的特異性或專一性(specificity) 11
1-2-4酵素的催化特性 12
1-2-5酵素之溫度效應 12
1-2-6酵素之pH值效應 14
1-2-7溶劑對酵素活性的影響 15
1-2-8固定化酵素(immobilized enzymes) 15
1-2-9固定化酵素的應用 24
1-3碳六十 29
1-3-1碳六十(C60)的發現 29
1-3-2碳六十性質 31
1-3-3碳六十的化學反應 34
1-3-4碳六十的有機化學反應 35
1-3-5碳六十的無機化學反應 36
1-3-6碳六十的應用 36
1-3-7碳六十在高溫超導的應用 37
1-3-8碳六十在藥物上的運用 38
1-4電化學生化感測器 38
1-4-1電化學生化感測器之定義 38
1-4-2一般電化學生化感測器種類) 39
1-5安培法葡萄糖生化感測器之偵測原理 39
1-6過渡金屬混價化合物 40
1-6-1簡介 40
1-6-2過渡金屬混價化合物之一般應用 43
1-6-3過渡金屬黃血鹽混價化合物在電化學之應用 43
第二章實驗部分 47
2-1儀器及藥品 47
2-2電極之製備 48
2-3操作條件之探討 52
2-3-1外加電位之探討 52
2-3-2攪拌速率之探討 52
2-3-3 pH值效應探討 52
2-3-4溫度效應探討 53
2-4反應時間及偵測範圍 53
2-4-1電極反應時間之探討 53
2-4-2電極偵測極限及glucose線性範圍之探討 53
2-4-3干擾物質對此系統之影響 54
2-4-4電極再現性之探討 54
2-5電極穩定性及壽命之探討 55
2-6自製碳電極 55
2-7系統示意圖 56
第三章 結果與討論 60
3-1碳六十-鈷赤血鹽修飾電極催化過氧化氫還原 60
3-1-1鈷赤血鹽之探討 60
3-1-2鈷赤血鹽催化能力之探討 62
3-1-3修飾方法及修飾物質之探討 66
3-1-4操作電位之探討 80
3-1-5攪拌速度之探討 82
3-1-6 pH及溫度之探討 82
3-1-7反應時間之探討 83
3-1-8 葡萄糖濃度線性範圍之探討 89
3-1-9干擾物質之影響 91
3-1-10再現性之探討 91
3-1-11電極穩定性及壽命之探討 99
3-2自製碳電極探討 99
第四章 結論 103

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