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研究生:蘇政宏
研究生(外文):Su Cheng-hung
論文名稱:厚朴、防己之成分與基原分析研究
指導教授:許順吉許順吉引用關係
指導教授(外文):Sheu Shuenn-jri
學位類別:碩士
校院名稱:國立臺灣師範大學
系所名稱:化學研究所
學門:自然科學學門
學類:化學學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:中文
論文頁數:235
中文關鍵詞:厚朴防己基原高效能液相層析毛細管電泳高效能液相層析質譜儀
外文關鍵詞:Magnoliae CortexfangchiresourceHPLCCELC-MS
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高效液相層析(HPLC)及毛細管電泳(CE)同屬液相分離技術,具有多種分離模式,為目前最常用於定量中藥材指標成分的分析方法;而近年來高效液相層析質譜儀(LC-MS)在中草藥的分析上逐漸扮演著重要的角色,本研究應用這三種儀器於厚朴、防己藥材的分析。LC-UV及CE-UV的偵測極限有一定的範圍,對藥材微量成分的分析,常有困難,本文開發毛細管電泳掃集法,成功地測定廣防己藥材中馬兜鈴酸的含量。
本研究分三個部分,第一部分為開發厚朴藥材的分析方法。厚朴為一芳香化濕藥,主要由酚類、精油及多種微量生物鹼組成。市售藥材主要有厚朴及和厚朴(Magnolia obovata THUNB.)兩種。其中厚朴又可分為川厚朴(M. officinalis REHD.et WILS.)及凹葉厚朴[M. officinalis ssp. Biloba(REHD.et WILS.)Law]兩種。本研究以七個生物鹼成份(1,(-)-magnocurarine,2,(+)-magnoflorine,3,(+)-laurifoline,4,(+)-oblongine,5, (+)-menisperine,6(+)-xanthoplanine,7(+)-N-methylglaucine)及兩個酚類成分(8,Honokiol,9,Magolol),共九個化合物為指標,利用高效能液相層析(HPLC)開發厚朴藥材成分的分析方法。實驗結果顯示,厚朴藥材經酸鹼處理後,利用過氯酸鹽及氰甲烷水溶液為衝提液,在波長280mm偵測下,可在60分鐘內成功地分析出這九個成分。本研究進一步利用LC-MS來確認厚朴藥材中指標成分存在的真實性。
第二部分為運用該分析方法於市售厚朴藥材之基原辨識及炮製、安定性之研究。市售厚朴藥材28批,用HPLC分析七個生物鹼成份及兩個酚類成分之含量。研究顯示,以M/H之比值,可以初步區分正品、偽品之厚朴,再利用七個生物鹼成分之間含量比值的差異,加強化學辨識的真實性。炮製方面,醋炒及薑炒對各成分的影響最大,而且在時間上也較為經濟。安定性研究方面,結果顯示,加熱溫度越高,對藥材的保存越不利。
第三部分為開發防己藥材之HPLC分析方法。市售防己藥材主要有防己科的日防己、粉防己、木防己外,還有馬兜鈴科的廣防己,防己藥材具利水消腫、祛風止痛的功效。本研究利用醋酸銨、醋酸和氰甲烷混合液為沖提液,在60分鐘內分析出四種防己的十五個成分,該條件亦適用於LC-MS系統。此外,利用此分析方法對四種防己之基原加以區分。以馬兜鈴酸來區分馬兜鈴科的廣防己;以sinomenine來區分日防己;以tetrandrine來區分粉防己;以magnoflorine來區分木防己。
最後,本文運用兩種毛細管電泳技術來分析廣防己藥材中馬兜鈴酸的含量。在rev. EOF CZE方面,利用陽離子界面活性劑(CTAB)和有機修飾劑之緩衝溶液,可在15分鐘內分析兩種馬兜鈴酸;在Sweeping方面,以70 mM SDS之50 mM 磷酸溶液,及氰甲烷為緩衝溶液( 75:25,v/v ),在30分鐘內能完全分離出二成分峰,再現性良好,而且可大幅降低分析的偵測極限。

Both high-performance liquid chromatography (HPLC) and capillary electrophoresis (CE) belong to the liquid-chromatography technology. They possess a number of separation modes and are currently the methods most commonly used .in assaying the marker substances of Chinese herb drugs. In current years, liquid chromatograph-mass spectrograph (LC-MS) gradually plays an important role. In this study we use these three instruments in analyzing the herb drugs of the magnolia and fangchi items. LC-UV and CE-UV have limited detection scopes and it is usually difficult for them to analyze trace elements. This study has successfully used CE-Sweeping method in detecting the content of aristolochic acid in Aristolochiae Fangchi Radix. .
The present study includes three parts. The first part is on the development of analysis methods for analyzing Magnoliae Cortex that is an aromatic dehumidifying Chinese herb drug containing chiefly phenols, essential oil and trace amounts of alkaloids. Commercial samples include Magnoliae Cortex and Magnoliae Obovatae Cortex. The former is also divided into Suchuanese magnolia (Magnoliae Officinales Radix) and concave-leaf magnolia (Magnoliae Officinales Ssp. Bilobae Radix). The present study takes nine compounds as the drug’s marker substances, which include seven alkaloids (1. (-)-magnocurarine; 2. (+)-magnoflorine; 3. (+)-laurifoline; 4. (+)-oblongine; 5. (+)-menispermine; 6. (+)-xanthoplanine; 7. (+)-N-methylglaucine) and two phenols (8. honokiol; 9. magnolol). High-performance liquid chromatography is utilized in developing the methods for analyzing the constituents of Magnoliae Cortex. Experimental results show that after acid and alkaline processing, and using perchlorate salt and acetonitrile-water solution as an eluent, the nine constituents can be analyzed at 280 mm within 60 min. In this study, LC-MS is also used to further confirm the reality of existence of the marker substances in Magnoliae Cortex.
The second part uses this method for identifying the origin and for the study on the processing and stability of the herb drugs. Twenty-eight batches of the commercial articles of Magnoliae Cortex are assayed for the contents of seven alkaloids and two phenols. Results show that by means of the M/H ratio, we are able to discriminate the orthodox from the counterfeit samples. Then by comparing the differences of ratios between the contents of the seven alkaloids, the validity of chemical identification can be augmented. In terms of stability study, the results show that the higher temperature the herb is subjected to during processing, the worse its preservation quality will be.
The third part deals with the development of methods for analyzing the herbs of the fangchi item by HPLC. Commercial samples of the fangchi item chiefly include those derived from the Menispermaceous Sinomenium acutum, Stephania tetrandra and Cocculus trilobus, and those from the Artistolochiaceous Aristolochia fangchi and Aristolochia heterophylla. Herb drugs of the fangchi item possess diuretic, detumescent, anemofugic and analgesic effects. This study uses a mixture of ammonium acetate, acetic acid and acetonitrile as an eluent to analyze 15 constituents from four samples of fanchi within 60 minutes. The condition is also applicable to the LC-MS system. Besides, This method can also be used for identifying the four fangchi samples, using aristolochic acid to identify Aristolochiae Fangchi Radix, sinomenine to identify Sinomenii Acuti Radix, tetrandrine to identify Stephaniae Tetrandrae Radix and magnoflorine to identify Cocculi Triobi Radix.
Finally, this study uses two CE techniques in analyzing the contents of aristolochic acid in samples of Aristolochiae Fangchi Radix. In the regard of rev. EOF CZE, with the use of anionic surfactant (CTAB) and organic modifier as a buffer, two kinds of aristolochic acid can be analyzed within 15 min. In the aspect of the Sweeping method, using a buffer made of 70 mM SDS in 50 mM phosphoric acid and acetonitrile (75 : 25, v/v), we can have two constituent peaks eluted out within 30 min. with good reproducibility, thereby lowering the detection limits of analyses to a very great extent.

圖目錄…………………………………………………………II
表目錄……………………………………………………...…VI
中文摘要……………………………………………………...IX
英文摘要………………………………………………...……XI
第一章 緒論
第一節 前言……………………………………………………………1
第二節 高效能液相層析………………………………………………2
第三節 液相層析/電灑/質譜儀………………………………………..3
第四節 毛細管電泳分析………………………………………………6
第五節 分析條件參數及適宜性之評估……………………………..16
第六節 毛細管電泳法和高效能液相層析法之比較………………..19
第二章 厚朴成分之HPLC分析方法開發
第一節 厚朴藥材檢液之前處理與標準品之分離………….……….21
第二節 厚朴成分之高效能液相層析分析…………………………..34
第三節 厚朴成分之LC-MS定性分析………………….………..….62
第三章 厚朴之基原、炮炙及安定性
第一節 厚朴基原之化學辨識………………………………………..79
第二節 厚朴藥材之炮炙及安定性研究……………………………106
第四章 防己成分之分析
第一節 防己成分之高效能液相層析分析…………………………121
第二節 防己成分之LC-MS定性分析……………………………..152
第三節 防己基原之化學辨識………………………………………186
第四節 廣防己及相關製劑中之馬兜鈴酸的毛細管電泳分析……………………………………………………………186
第五章 結論…………………………………………………………..215
參考資料………………………………………………………………226
附錄一 厚朴分析成分UV圖………..……………………………236
附錄二 防己分析成分UV圖………..……………………………241
附錄三 防己顯微特徵圖………..…………………………………..247

[1] M. Twett, Proc. Warsaw Soc. Nat. Sci. Biol., 1903, 14, 6.
[2] A. J. P. Martin and R. L. M Synge, Biochem. J., 1941, 35, 1358.
[3] A. T. James and A. J. P. Martin, Analyst, 1952, 77, 915.
[4] E. Stahl, Chemiker-Ztg., 1958, 82, 323.
[5] J. F. K. Huber and J. A. R. J. Hulsman, Anal. Chim. Acta, 1967, 38, 305.
[6] J. Zeleny, Phys. Rev., 1917, 10, 1.
[7] W. M. A. Niessen and J. V. D. Greed, Chromatographic Science Series, Vol. 58:Liquid Chromatography-Mass Spectrometry. Principle and Applications, chapter 3, M. Dekker, New York, 1992.
[8] M. Dole, L. L. Mack, R. L. Hines, R. C. Mobley, L. D. Ferguson and M. B. Alice, J. Chem. Phys., 1968, 49, 2240.
[9] M. Yamashita and J. B. Fenn, J. Phys. Chem., 1984, 88, 4451.
[10] M. Yamashita and J. B. Fenn, J. Phys. Chem., 1984, 88, 4671.
[11] C. M. Whitehouse, R. N. Dreyer, M. Yamashita and J. B. Fenn, Anal. Chem., 1985, 57, 675.
[12] P. Kebarle and L. Tang, Anal. Chem., 1993, 65, 972A.
[13] M. G. Ikonomou, A. T. Blades and P. Kebarle, Anal. Chem., 1991, 63, 1989.
[14] V. J. Iribarne and B. A. Thomosm, J. Chem. Phys., 1976, 64, 2287.
[15] G. J. V. Berkel, S. A. McLuckey and G. L. Glish, Anal. Chem., 1992, 64, 1586.
[16] A. Tiselius, Tran. Faraday Soc., 1937, 33, 524.
[17] S. Hjertén, Chromatogr. Rev., 1967, 9, 122.
[18] R. Virtanen, Acta Polytechnica Scand., 1974, 1, 123.
[19] F. E. P. Mikkers, F. M. Everaerts and Th. P. E. M. Verheggen, J. Chromatogr., 1979, 169, 11.
[20] J. W. Jorgenson and K. D. Lukacs, Anal. Chem., 1981, 53, 1298.
[21] S. Hjertén, J. Chromatogr., 1985, 347, 191.
[22] J. R. Mazzeo and I. S. Krull, BioTechniques, 1991, 10, 638.
[23] T. Wehr, LC-GC, 1993, 11, 14.
[24] A. S. Cohen and B. L. Karger, J. Chromatogr., 1987, 397, 409.
[25] A. S. Cohen, A. Paulus, and B. L. Karger, Chromatographia, 1987, 24, 15.
[26] A. S. Cohen, D. R. Najarian, A. Paulus, A. Guttman, J. A. Smith and B. L. Karger, Proc. Natl. Acad. Sci. U.S.A., 1988, 85, 9660.
[27] H. Drossman, J. A. Luckey, A. Kostichka, J. D’Cunha and L. M. Smith, Anal. Chem., 1990, 62, 900.
[28] P. Bocek and A. Chrambach, Electrophoresis, 1991, 12, 1059.
[29] S. Terabe, K. Otsuka, K. Ichikawa, A. Tsuchiya and T. Ando, Anal. Chem., 1984, 56, 111.
[30] S. Terabe, K. Otsuka and T. Ando, Anal. Chem., 1985, 57, 834.
[31] S. Hjertén, J. L. Liao and K. Yao, J. Chromatogr., 1987, 387, 127.
[32] D. J. Rose and J. W. Jorgenson, Anal. Chem., 1988, 60, 642.
[33] D. N. Heiger,高效毛細管電泳導論(中譯本),中國惠普公司,北京,1993。
[34] T. Tsuda, Handbook of Capillary Electrophoresis; J. P. Landers, ed. , Chapter 1, CRC Press, Boca Raton, 1997.
[35] H. Z. Helmholtz, Annal. Phys. Chem., 1879, 7, 337.
[36] K. Shibata, S. Iwata and M. Nakamura, Acta Phytochim., 1923, 1, 105.
[37] S. Hjertén and M. D. Zhu, J. Chromatogr., 1985, 346, 265.
[38] S. Hjertén and M. D. Zhu, J. Chromatogr., 1985, 327, 157.
[39] S. Hjertén and M. D. Zhu, Protides of the Biological Fluids, pp. 537-540, Oxford, New York, 1986.
[40] S. Hjertén, K. Elenbring F. Kilár, J. L. Liao, A. J. C. Chen, C. J. Siebert and M. D. Zhu, J. Chromatogr., 1987, 403, 47.
[41] E. M. Everaerts and P. E. M. Verheggen, New Directions in Electrophoretic Methods-Am. Chem. Soc. Symp. Vol. 335, J. W. Jorgenson and M. Phillips, ed., Chap. 4, American Chemical Society, Washington, D. C., 1987.
[42] J. W. Jorgenson and K. D. Lukacs, J. Chromatogr., 1981, 218, 209.
[43] J. W. Jorgenson and K. D. Lukacs, J. High Resoln Chromatogr., Chromatogr. Comm., 1981, 4, 230
[44] J. W. Jorgenson and K. D. Lukacs, Clin. Chem., 1981, 27, 1551.
[45] J. W. Jorgenson and K. D. Lukacs, Science, 1983, 222, 266.
[46] J. W. Jorgenson, Trends Anal. Chem., 1984, 3, 51.
[47] K. D. Altria and C. S. Simpson, Anal. Proc., 1986, 23, 453.
[48] T. Tsuda, J. High Resoln Chromatogr., Chromtogr. Comm., 1987, 10, 622.
[49] S. Terabe, K. Otsuka and T. Ando, Anal. Chem., 1989, 61, 25l.
[50] K. Otsuka and S. Terabe, J. Microcol. Sep., 1989, 1, 150.
[51] T. Tsuda, K. Nomura and G. Nakagawa, J. Chromatogr., 1982, 248, 241.
[52] M. J. Sepaniak and R. O. Cole, Anal. Chem., 1987, 59, 472.
[53] T. Balchunas and M. J. Sepania, Anal. Chem., 1988, 60, 1466
[54] M. Martin, G. Guiochon, Y. Walbroehl and J. W. Jorgenson, Anal. Chem., 1985, 57, 559.
[55] M. M. Bushey and J. W. Jorgenson, J. Microcol. Sep., 1989, 1, 125.
[56] A. Dobashi, T. Ono, S. Hara and J. Yamaguchi, J. Chromatogr., 1989, 480, 413.
[57] S. Terabe, H. Utsumi, K. Otsuka, T. Ando, T. Inomata, S. Kuze and Y. Hanaoka, J. High Resoln Chromatogr. Chromatogr., Commun., 1986, 9, 666.
[58] D. E. Burton, M. J. Sepaniak and M. P. Maskarinec, J. Chromatogr. Sci., 1987, 25, 514.
[59] J. C. Reijenga, G. U. A. Aben, T. P. E. M. Verheggen and F. M. Everaerts, J. Chromatogr. , 1983, 260, 241.
[60] T. Tsuda, J. High Resoln Chromatogr. Chromatogr., Commun., 1987, 10, 622.
[61] W. R. Jones and P. J Andik, J. Chromatogr. , 1991, 546, 445.
[62] W. R. Jones and P. J Andik, J. Chromatogr. , 1992, 608, 385.
[63] J. P. Quirino and S. Terabe, Science, 1998, 282, 465.
[64] J. P. Quirino and S. Terabe, Anal. Chem., 1999, 71, 1638.
[65] C. X. Zhang and W. Thormann, Anal. Chem., 1998, 70, 540.
[66] Z. K. Shihabi, J. Chromatogr. A, 1998, 817, 25.
[67] J. Palmer, N. J. Munro and J. P. Landers, Anal. Chem., 1999, 71, 1679.
[68] P. Britz-McKibbin, G. M. Bebault and D. Y. Chen, Anal. Chem., 2000, 72, 1729.
[69] P. Britz-McKibbin and D. Y. Chen, Anal. Chem., 2000, 72, 1242.
[70] J. Palmer and J. P. Landers, Anal. Chem., 2000, 72, 1941.
[71] J. Palmer, N. J. Munro, D. S. Burgi and J. P. Landers, Anal. Chem., 2001, 73, 725.
[72] J. P. Quirino and S. Terabe, Anal. Chem., 2000, 72, 1023.
[73] 許鴻源、陳玉盤、許順吉、許照信、陳建志、張憲昌,簡明藥材學,p.207,新醫藥出版社,台北,1985.
[74] K. Ougi and M. Morita, J. Pharmacol.,(Japan), 1953, 2, 89 ; Chem. Abstr., 1953, 47, 10128b.
[75] 上海第一醫學院藥學系醫學基礎教研組,新醫藥學雜誌(第四卷),p.31,上海人民出版社,上海,1973.
[76] K. Shimamoto, K. Inoue and K. Ougi, J. Pharmacol., (Japan),
1958, 7, 135 ; Chem. Abstr., 1958, 52, 18896i.
[77] G. Zhou, Z. Zhu and R. Li, Yaoxue Tongbao, 1981, 16, 587;Chem. Abstr., 1981, 96, 214277b.
[78] D. Chen and J. Liu, Yaoxue xuebao, 1982, 17, 360;Chem. Abstr., 1982, 97, 78974t.
[79] A. Li, X. Guo, X. Wang, C. Chen, Y. Shi, N. Sui and J. Du, Yaowu Fenxi Zazhi, 1983, 3, 1;Chem. Abstr., 1983, 99, 1092j.
[80] X. Ou, L. Zhang and Y. Xi, Yaoxue Tongbao, 1984, 19, 421;Chem. Abstr., 1984, 102, 100825x.
[81] A. Li, X. Guo, X. Wang and C. Chen, Yaowu Fenxi Zazhi, 1984, 4, 271;Chem. Abstr., 1984, 101, 235655k.
[82] E. Katsura, T. Yamagishi, S. Yahara and I. Nishioka, Hokkaidoritsu Eisei Kenkyushoho, 1984, 34, 24;Chem. Abstr., 1984, 103, 11529a.
[83] G. Zhou and Z. Zhu, Zhongcaoyao, 1985, 16, 104;Chem. Abstr., 1985, 102, 225903w.
[84] J. Cui, G. Zhang and W. Song, Yaowu Fenxi Zazhi, 1988, 8, 274;Chem. Abstr., 1988, 110, 63817g.
[85] Z. Li, M. Zhang, X. Lin and Y. Hou, Zhongguo Zhongyao Zazhi, 1989, 14, 399;Chem. Abstr., 1989, 111, 201712t.
[86] L. Hu, M. Sha, B. Fu and L. Zhao, Zhongguo Zhongyao Zazhi, 1991, 16, 535;Chem. Abstr., 1991, 116, 46390.
[87] Z. Zhao, M. Hu and X. Tang, Zhongguo Zhongyao Zazhi, 1992, 17, 15;Chem. Abstr., 1992, 116, 211143.
[88] C. Ye, Y. Feng and J. Teng, Yaowu Fenxi Zazhi, 1992, 12, 159;Chem. Abstr., 1992, 117, 76583x.
[89] Q. Yu and J. Sheng, Zhongguo Zhongyao Zazhi, 1992, 17, 404;Chem. Abstr., 1992, 117, 239919c.
[90] S. Su, C. Sui, C. Xu, R. Sun, Y. Wang, H. Liu and X. Shen, Shengyang Yaoxueyuan Xuebao, 1992, 9, 185;Chem. Abstr., 1992, 119, 125293e.
[91] T. H. Tsai and C. F. Chen, J. Chromatogr., 1992, 598, 143.
[92] P. Ma and T. Lin, Huaxi Yaoxue Zazhi, 1993, 8, 87;Chem. Abstr., 1993, 121, 187395.
[93] N. Momma and K. Ohsawa, Yakugaku Zasshi, 1993, 113, 155;Chem. Abstr., 1993, 119, 80345e.
[94] P. Ma and T. Lin, Huaxi Yaoxue Zazhi, 1993, 8, 87;Chem. Abstr., 1993, 121, 187395q.
[95] Q. Zeng, X. Song, Q. Zhang, W. Tu and L. Zhang, Zhongcaoyao, 1996, 27, 11;Chem. Abstr., 1996, 125, 67932.
[96] J. Ueda, N. Asaka, I. Tanaka, Y. Hayashi, Y. Hirose, N. Momma, T. Yasuda and K. Ohsawa, Yakugaku Zasshi, 1993, 113, 894;Chem. Abstr., 1997, 120, 280393.
[97] K. H. Bae, H. H. Kim, D. H. Won, J. S. Lee and J. S. Kang, Yakhak Hoechi, 1997, 41, 407;Chem. Abstr., 1997, 127, 283436.
[98] Z. Wu, L. Kuang, G. Zhang, Z. Pan, F. Li and X. Hu, Zhongguo Zhongyao Zazhi, 1998, 23, 223;Chem. Abstr., 1999, 130, 122156.
[99] J. F. Cui, G. D. Zhang and W. Z. Song, Yaoxue Xuebao, 1988, 23, 383;Chem. Abstr., 1988, 109, 79805s.
[100] W. Song, J. Gui and G. Zhang, Fitoterapia, 1990, 61, 245;Chem. Abstr., 1990, 114, 139727.
[101] W. Song, J. Cui and G. Zhang, Tianran Chanwu Yanjiu Yu Kaifa, 1990, 2, 1;Chem. Abstr., 1990, 114, 192695.
[102] M. Yao, L. Chen, Y. Dai, W. Liu and Z. Xian, Zhongcaoyao, 1991, 22, 351;Chem. Abstr., 1991, 116, 18397.
[103] F. J. Wu and S. J. Sheu, Zhonghua Yaoxue Zazhi, 1992, 44, 317.
[104] S. Su, C. Xu, C. Sui, R. Sun, Y. Wang, H. Liu and X. Shen, Shenyang Yaoxueyuan Xurbao, 1992, 9, 185;Chem. Abstr., 1992, 119, 125293.
[105] C. Zhao, J. Huang and M. Zhou, Huazhong Nongye Daxue Xuebao, 1994, 13, 373;Chem. Abstr., 1994, 122, 310715.
[106] M. Moriyasu, Y. Nishiyama, M. Ichimaru and A. Kato, Nat. Med., 1994, 48, 282.
[107] Y. He, M. Wu, B. Cai, P. Zheng and Z. Yuan, Zhongguo Zhongyao Zazhi, 1995, 20, 84;Chem. Abstr., 1995, 122, 274194n.
[108] M. Moriyasu, J. Wang, H. Zhang, G. B. Lu, M. Ichimaru and A. Kato, Nat. Med. (Tokyo), 1996, 50, 413.
[109] R. Ziyaev, N. I. Shtonda, A. Abdusamatov and D. M. Tsakadze, Chem. Nat. Comp., 1999, 35, 366;Chem. Abstr., 2000, 132, 205497.
[110] N. Okamura, H. Miki, T. Harada, S. Yamashita, Y. Masaoka, Y. Nakamoto, M. Tsuguma, H. Yoshitomi and A. Yagi, J. Pharm. Biomed. Anal., 1999, 20, 363.
[111] 江慧玉,麻黃、厚朴、吳茱萸之毛細管電泳及高效能液相層析研究,國立台灣師範大學化學研究所,碩士論文,台北,2001.
[112] 同[106]
[113] 同[108]
[114] 同[73]
[115] 許鴻源,中藥材之研究,p.68,新醫藥出版社,台北,1980.
[116] 甘偉松,藥用植物學,p.224,國立中國醫藥研究所,台北,1979.
[117] 顏焜熒,常用中藥之藥理vol.Ⅱ,p.6,國立中國醫藥研究所,台北,1970.
[118] 清,寇宗奭,本草衍義,p.79,商務印刷館,北京,1959.
[119] 徐珞珊、徐國鈞、金蓉鸞、何宏賢,中國藥材學,p.784,中國醫藥科技出版社,北京,1994.
[120] S. Takagi, J. Pharm. Soc. (Japan), 1921, 473, 565.
[121] Y. Sugii and H. Shindo, J. Pharm. Soc. (Japan), 1930, 50, 709.
[122] Y. Sugii, Ber. 1930, 50, 183 ; Chem. Abstr. ,1930, 24, 3505.
[123] M. Tomita, Y. Inubushi and M. Yamagata, J. Pharm. Soc. (Japan),1951, 71, 1069; Chem. Abstr. 1952, 46, 5059h.
[124] M. Tomita and T. Nakano, Ber., 1952, 72, 197 ; Chem. Abstr. 1953, 47, 1627i.
[125] M. Tomita and T. Nakano, Ber., 1952, 72, 1256 ; Chem. Abstr. 1953, 47, 12287i.
[126] T. Nakano, Pharm. Bull. (Japan), 1954, 2, 329 ; Chem. Abstr. 1956, 50, 7117h.
[127] T. Nakano, Chem. Pharm. Bull., 1956, 4, 408.
[128] M. Tomita and M. Kozuka, Yakugaku Zasshi, 1967, 87, 1134.
[129] M. Fujita, H. Itokawa and Y. Sashida, Yakugaku Zasshi, 1973, 93, 429.
[130] 葛發歡,中藥材,1990, 13, 45.
[131] 曹治儀、陳芳群,中草藥,1981, 12, 5.
[132] 崔建芳,藥學學報,1988, 23, 383.
[133] 曹治儀、陳芳群,中草藥,1985, 16, 2.
[134] 度邊和夫,中醫中藥分冊(第三冊),p.54,國外醫學,1981.
[135] 吳葆杰,中草藥藥理學,p.133,人民衛生出版社,北京,1983.
[136] 王浴生,中藥藥理與應用,p.770,人民衛生出版社,北京,1983.
[137] 江蘇新醫學院,中藥大辭典(下冊),p.1665,上海人民出版社,上海,1977.
[138] T. Namba, M. Tsunezuka and M. Hattori, Planta Med.,1982, 44, 100 ; Chem. Abstr., 1982, 96, 223080j.
[139] K. Inoue, Nippon Yakurigaku Zasshi, 1957, 53, 797; Chem. Abstr., 1958, 52, 18870b.
[140] 上海中醫學院藥理教研組,新醫藥學雜誌(第五卷),p.41,上海人民出版社,上海,1974.
[141] 同[76]
[142] T. Konoshima, J. Nat. Prod., 1991, 54, 816.
[143] T. Konoshima, Planta Med., 1990, 56, 653.
[144] 同[75]
[145] 度邊和夫,中醫中藥分冊(第三冊),p.54,國外醫學,1981.
[146] M. Fujita, H. Itokawa and Y. Sashida, Yakugaku Zasshi, 1973, 93, 415.
[147] K. Watanabe, J. Pharmacol., (Japan), 1975, 25, 605 ; Chem. Abstr., 1976, 84, 99380t.
[148] M. Fujita, H. Itokawa and Y. Sashida, Yakugaku Zasshi, 1973, 93, 429.
[149] S. Yahara, T. Nishiyori, A. Kohda, T. Nohara and I. Nishioka, Chem. Pharm. Bull., 1991, 39, 2024;Chem. Abstr., 1991, 116, 67005.
[150] M. Ogata, M. Hoshi, K. Shimotohno, S. Urano and T. Endo, J. Am. Oil Chem. Soc., 1997, 74, 557;Chem. Abstr., 1997, 127, 28634.
[151] Z. P. Zhang, Z. D. Hu and G. L. Yang, Mikrochim. Acta, 1997, 127, 253;Chem. Abstr., 1997, 127, 253045.
[152] H. Zhang, Z. Hu, G. Yang, Z. Shi and H. Sun, Anal. Lett., 1997, 30, 2327.
[153] Q. Shen and Z. Fang, Fenxi Shiyanshi, 1998, 17, 1.
[154] Y. Maruyama and H. Kuribara, CNS Drug Rev.,2000, 6, 35; Chem. Abstr., 2000, 133, 114439.
[155] J. F. Cui, G. D. Zhang and W. Z. Song, Yaoxue Xuebao, 1988, 23, 383;Chem. Abstr., 1988, 109, 79805s.
[156] J. Zhu, C. Heo, M. Wei, X. Wang and X. Luo, Fenxi Ceshi Tongbao, 1988, 7, 20;Chem. Abstr., 1988, 110, 141638.
[157] Y. Q. Zhu, Y. Y. Zeng, X. P. Pan, J. P. Si and Z. L. Wang, Zhejiang Linxueyuan Xuebao, 1999, 16, 387.
[158] K. Kondo, N. Hattori, K. Kazushige, I. Sakakibara, S. Terabayashi, M. Kubo, M. Higuchi, H. Sasaki, H. Kamei, M. Okada, G. M. Shu, X. M. Xu and X. Z. Liu, Nat. Med. (Tokyo), 2000, 54, 61.
[159] D. Chen and J. Liu, Yaoxue Xuebao, 1982, 17, 360 ; Chem. Abstr., 1982, 97, 78974t.
[160] 李美貴、凌永健,科儀新知,第十三卷第五期,pp.38-52,行政院國家科學委員會精密儀器發展儀器中心,新竹1992.
[161] J. L. Wolfender and K. Hostettmann, J. Chromatogr. , 1993, 647, 191.
[162] M. A. Aramendia, I. Garcia, F. Lafont and J. M. Marinas, J. Chroma-
togr. A , 1995, 707, 327.
[163] 宋,唐慎微,重修政和經史證類備用本草,p.324,人民衛生出版社,北京,1982.
[164] 同[118],p.79.
[165] 全國中草藥匯編編寫組,全國中草藥匯編(上冊),第一版,p.394,人民衛生出版社,北京,1976.
[166] 宋萬志,藥學學報,1984,19,213.
[167] 宋萬志、童玉懿、王珂、馬林、吳豐、劉永隆,常用中藥材品種整理和質量研究(北方編),第二冊,p.211,徐國鈞、徐珞珊編,福建科學技術出版社,福建,1994.
[168] 謝萬宗,中藥材品種論述(中冊),p.17,上海科學技術出版社,上海,1959.
[169] 行政院衛生署中醫藥委員會網站:http://www.ccmp.gov.tw/
[170] 許鴻源,中藥之炮炙,第17-22頁,新醫藥出版社,台北,1980。
[171] 詹逸州,中藥材芍藥藥效之定量研究,國立台灣師範大學化學研究所碩士論文,台北,1993。
[172] 郭千慧,中藥材羌活藥效成分之定量研究,國立台灣師範大學化學研究所碩士論文,台北,1994。
[173] 吳權益,大黃葛根黃芩之定量分析研究,pp.100-119,國立台灣師範大學化學研究所碩士論文,台北,2001。
[174] 蔡蕓,中藥材安定性-白朮儲存之研究,台北醫學院生藥學研究所碩士論文,台北,1997。
[175] 吳鳳珍,厚朴與枳實藥效成分之定量及厚朴酚相關化合物之合成,pp.44-57,國立台灣師範大學化學研究所碩士論文,台北,1987。
[176] 葉政洳,黃芩成分之分析與基原之辨識,pp.93-103,國立台灣師範大學化學研究所碩士論文,台北,2000。
[177] 蕭天石,神農本草經,pp.160-161,自由出版社,台北,1988。
[178] 明 李時珍,本草綱目,卷十八,pp.716,國立中國醫藥研究所,台北,1976。
[179] 全國中草藥匯編編寫組,全國中草藥匯編(上冊),第一版,pp.
657-658,人民衛生出版社,北京,1992。
[180] 許鴻源,中藥成分最近研究,p.55,國立中國醫藥研究所,台北,1968。
[181] 罔西為人,重輯新修本草,p.219,國立中國醫藥研究所,台北,1964。
[182] 許信義、張憲昌、許鴻源,市售防己類藥材之生藥學研究,pp.
33-45,中國醫藥研究所叢刊社,台北,1986。
[183] 謝宗萬,中藥材品種論述(上),pp.242-245,上海科學技術出版社,上海,1990。
[184] 中國藥品生物製品檢定所,中國科學院植物研究所編,中藥鑑別手冊(一),pp.230-234、286,科學出版社,北京,1993。
[185] V. Jean Louis, Lancet, 1993, 341, 387.
[186] 鄧正賢,台灣防己類藥材鑑別與品質評價之研究,pp.26-28,國立中國醫藥研究所,台北,1995。
[187] 徐學健、陳嬿,中藥材,1987, 18(4), 2.
[188] 阮德媋、張雪梅、趙春景、王方才、田利孝,雲南植物研究,1991, 13(2), 225.
[189] 黃建明、郭濟賢、段更利,藥學學報,1988, 33, 528.
[190] R. S. Singh, P. Kumar and D. S. Bhakuni, J. Nat. Prod., 1981, 44, 664.
[191] 張永勳,台灣市售防己、牛膝類藥材及含其製劑之研究,第十五期,第二冊,pp.4-14,中國醫藥年報,行政院衛生署中國醫藥委員會編印,台北,1997。
[192] Y. Inubushi, Y. Ito, Y. Masaki and T. Ibuka, Chem. Pharm. Bull., 1997, 25, 1636.
[193] 郭幼莞、符小文,藥學學報,1999, 34, 690.
[194] S. A. Philipov and R. S. Istatkova, Phytochemistry., 1997, 44, 1591.
[195] Y. Sugimoto, H. A. A. Babiker, T. Saisho,T. Furumoto,S. Inanaga and M. Kato, J. Org. Chem., 2001, 66, 3299.
[196] T. Tanahashi, Y. Su, N. Nagakura and H. Nayeshiro, Chem. Pharm. Bull., 2000, 48, 370.
[197] T. Furumoto, Y. Sugimoto, Planta. Med., 2001, 67, 194.
[198] 陳銘志,利用液相層析/電灑/質譜儀及毛細管電泳檢測防己樣品中之主要活性化合物,pp.34-47,私立東吳大學化學研究所碩士論文,台北,2000。
[199] 同[115]
[200] 同[186]
[201] 同[185]
[202] V. Jean Louis, Lancet, 1994, 343, 173.
[203] 同[198]

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