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研究生:黃榮松
論文名稱:阻力訓練與維生素E補充對男性血中抗氧化酉每、丙二醛、及肌酸激酉每的影響
論文名稱(外文):The Effects of Resistance Training and Vitamin E Supplementation on Blood Antioxidant Enzymes, MDA, and CK in Males
指導教授:方進隆方進隆引用關係
學位類別:博士
校院名稱:國立臺灣師範大學
系所名稱:體育研究所
學門:教育學門
學類:專業科目教育學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:中文
論文頁數:108
中文關鍵詞:麥夫 胱甘 月太丙二醛肌酸激 酉每維生素E阻力訓練
外文關鍵詞:GSHMDACKVitamin Erestistance training
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本研究的目的在於探討(1)一週阻力訓練與維生素E補充對血中 麥夫 胱甘月太 (GSH)、丙二醛(MDA)及肌酸激酉每 (CK)的影響;(2) 七週的阻力訓練與維生素E補充對血中GSH、MDA、CK及各項肌力的效果;(3)比較一週與七週訓練在GSH、MDA、CK效果上有何不同。
本研究以26位大學男性自願擔任受試者,經隨機抽樣分配至控制組(C組)、阻力訓練組(R組)及阻力訓練+維生素E組(E組)。阻力訓練以8RM、10個動作、三回合、每週三次訓練。維生素E補充量為每天400IU。受試者於訓練前(A期)、一週訓練完立即(B期)、七週訓練完立即(C期)及訓練結束後兩天(D期)抽血檢查 GSH、MDA及CK。雙因子變異數混合設計、單因子共變數分析各變項資料、薛費事後考驗其差異性;相依樣本t考驗A與D期、B與C期間各變相差異;顯著水準為=.05。研究結果如下:
(一) 一週阻力訓練方面:
1. 各組GSH濃度沒有差別,但E組有較低的趨勢。
2.三組的MDA在B期沒有差別。
3. R組在B期CK濃度明顯高於C組(P< .05),但R組與E組、E組與C組沒有差別。
(二)七週的阻力訓練方面:
1.C與D期E組GSH濃度明顯低於R組及C組(P<.05),但R與C組間沒有不同。
2. MDA三組於C期及D期沒有不同。
3.血中CK方面則各組在C期及D期間沒有不同。
(三)一週與七週阻力訓練效果比較:
1.血中GSH的變化情形:
(1) A與D期間:C組於D期明顯低(P<.05)於A期,R組與E組則沒有差別。B與C期間: E組GSH濃度於C期明顯低於B期(P<.05);而R與C組沒有差別。
(2) 短期的訓練E組雖沒有明顯的差異,但長期訓練則明顯較R組及C組低(P<.05)。
2.MDA的變化情形:三組在各不同抽血時段間沒有差別。
3.CK的變化情形:
(1)在A與D期間:訓練前的E組明顯較D期低,其他各組沒有不同。
(2)在B與C期間:R組於B期的CK濃度明顯高於C期(P<.05),其他各組間沒有差別。
(3) E組在B期一直處於比R組低的狀態,但C期則沒有這樣的現象。
(四)至於肌力方面:
阻力訓練有助於上半身及下半身肌力進步,而維生素E補充進步的情況有比較好的趨勢。
無論短期或是長期的阻力訓練都不致於使GSH改變,但在維生素E的補充下有降低的作用。而MDA濃度顯示阻力訓練後不會有脂質過氧化的發生。至於CK方面則於短期訓練後明顯較高,而長期訓練後則下降的情形。最後是阻力訓練有助於肌力增進且維生素E補充效果更佳。
The purposes of the study were to 1) test the effects of one week of resistance training and vitamin E supplementation on blood glutathione (GSH), malondiadehyde (MDA), and creatine kinase (CK), 2) investigate the effect of 7 weeks of resistance training and vitamin E supplementation on GSH, MDA, CK, and muscular strength, and 3) compare with the adaptive effects of plasma GSH, MDA, and CK between one week and 7 weeks of resistance training and vitamin E supplementation in males.
Twenty-six college male volunteers were randomly assigned into three groups including vitamin E supplementation plus resistance training (EG), resistance training only (RG), and control (CG) group. The subjects of the RG performed 10 exercises, 8 RM, 3 sets, 3 days/week for 7 weeks. The EG took 400 IU vitamin E per day and plus the same volume of resistance training of RG. Blood samples were collected at pre (A), immediately after one week of training (B) and after 7 weeks of training (C), and two days after the completion of all resistance training (D). Two-way ANOVA mixed design and one-way ANCOVA were used to test the difference among three groups on GSH, MDA, and CK at different periods. Scheffe post hoc comparisons were used to test the difference between groups. Paired-t tests were used to test the dependent variables between A-D and B-C period of blood sample obtained at the same group. The statistical significant level was set at .05. The findings were as follows:
1. The effect of one week of resistance training:
(1) No significant differences were found among three groups on plasma GSH; however, the EG had tendency of lower plasma GSH than that of the RG.
(2) No significant differences were found among three groups on plasma MDA.
(3) The RG had significantly higher (P< .05) plasma CK level at B period than the CG. No difference was found between EG and CG.
2. The effect of 7 weeks of resistance training:
(1) The plasma GSH level on EG was significantly lower (P < .05) at period of C and D than those of RG and CG. There was no difference of plasma GSH level between EG and CG.
(2) No difference of the plasma MDA level among three groups was found.
(3) No difference of the plasma CK level among three groups at periods C and D was found.
3. The effects of resistance training between one week and 7 weeks:
(1) The effect on plasma GSH:
A. The CG decreased (P < .05) between period of A and D. The EG at period of C was lower (P < .05) than EG at period of B. No difference between RG and CG was found.
B. No significant difference was found on the plasma GSH level among three groups after a week of resistance training; however, the GSH level of EG was significant lower (P < .05) than the RG and CG after 7 weeks of resistance training.
(2) The effect on plasma MDA: No difference among three groups between A and D period, B and C were found.
(3) The effect on plasma CK:
A. EG at period A was lower (P < .05) than that of at period D. No difference was found among three groups.
B. The plasma CK level of RG after one week of training was significantly higher than that of seven weeks of training (P < .05). No significant difference was found between EG and CG was found.
C. EG had a lower of plasma CK at B period; however, EG had no such a lower tendency at period C.
(4) Muscular strength significantly increased (P < .05) on the upper and lower body after 7 weeks of resistance training. Although no significant difference was found among groups of muscular strength, the Vitamin E supplementation group had better tendency on the improvement of muscular strength.
Based on the results, it was concluded that resistance training had no effects on the changes of the plasma GSH level. Furthermore vitamin E supplementation decreased plasma GSH after 7 weeks of resistance training. There was no lipid peroxidation since no plasma MDA changes occurred; meanwhile, resistance training with vitamin E supplementation did not change plasma MDA level. In addition, the blood CK was increased after a week of resistance training which support the theory that micro muscular trauma had occurred; however, long-term resistance training did not change the level of CK. Lastly, the resistance training improved muscular strength while training with vitamin E supplementation seems to have better results on strength increase.
摘要 I
英文摘要 II
誌謝詞 IV
目錄 V
表目次 VIII
圖目次 X
第壹章 緒論 1
一、 問題背景 1
二、 研究目的 5
三、 研究假設 6
四、 研究的必要性 6
五、 研究範圍與限制 7
六、 操作性定義 7
第貳章 文獻探討 9
一、 自由基的來源 9
(一)自由基的生成 9
(二)自由基的清除 11
(三)本節小結 14
二、 運動訓練與自由基 15
(一)動物實驗 15
(二)人體實驗 19
(三)本節小結 22
三、 阻力訓練與自由基 23
(一)阻力訓練與運動表現 23
(二)阻力訓練與氧化性壓力 25
(三)阻力訓練與維生素補充 29
(四) 本節小結 30
四、 維生素E的攝取與運動訓練 31
(一)維生素E的組成 31
(二)維生素E、氧化性壓力與運動表現 32
(三)人體實驗 33
(四)動物實驗 36
(五)本節小結 36
第參章 研究方法與步驟 38
一、 研究對象 38
二、實驗流程及步驟 38
三、 測驗方法 39
四、維生素E攝取 47
五、飲食紀錄 48
六、實驗控制 48
七、 統計資料處理 48
第肆章 結果 49
一、阻力訓練與維生素E補充對血中GSH的影響 49
二、阻力訓練與維生素E補充對血中MDA的影響 53
三、阻力訓練與維生素E補充對血中CK的影響 55
四、阻力訓練與維生素E補充各組間肌力方面 59
五、各組間血中維生素E的濃度 67
第伍章 討論 70
一、阻力訓練與維生素E補充對血中GSH的影響 70
二、阻力訓練與維生素E補充對血中MDA的影響 73
三、阻力訓練與維生素E補充對血中CK的影響 75
四、阻力訓練與維生素E補充各組間肌力方面 77
五、各組間血中維生素E的濃度 78
第陸章 結論與建議 80
參考文獻 83
附錄一 受試者同意書 96
附錄二 簡易食物代換表 104
附錄三 國內各種食品熱量對照表 105
附錄四 受試者活動行事曆 108
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