跳到主要內容

臺灣博碩士論文加值系統

(3.239.4.127) 您好!臺灣時間:2022/08/16 03:08
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果 :::

詳目顯示

我願授權國圖
: 
twitterline
研究生:蕭培澤
論文名稱:養殖海鱺血清中弧菌蛋白分解酵素抑制物之特性研究
論文名稱(外文):Studies on the characteristics of vibrio protease inhibitors in the serum of cobia, rachycentron canadum
指導教授:李國誥李國誥引用關係
學位類別:碩士
校院名稱:國立海洋大學
系所名稱:水產養殖學系
學門:農業科學學門
學類:漁業學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:中文
中文關鍵詞:海鱺蛋白質分解酵素抑制物弧菌
相關次數:
  • 被引用被引用:5
  • 點閱點閱:156
  • 評分評分:
  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:2
  本論文進行海鱺血清中能抑制弧菌蛋白質分解酵素活性之物質分離純化及特性研究。以自罹病海鱺所分離出的弧菌Vibrio. alginolyticus strain C11a及巴斯德桿菌Photobacterium damsela subsp. piscicida strain Cp兩菌株之細胞外產物為對象,以探討海鱺血清中存在之蛋白質分解酵素抑制物之特性。
  先以飽和度0~30﹪(A組)及50~70﹪(B組)硫酸銨將海鱺血清中大部分的蛋白質沈澱區分,再以疏水性作用層析管柱、親和力層析管柱、陰離子交換法等管柱進行海鱺血清中蛋白質分解酵素抑制物質之分離純化,以Native-PAGE電泳分析,A組出現兩條明顯的蛋白帶,B組則出現一條蛋白帶,再以zymogram確認具抑制蛋白質分解酵素活性之蛋白帶。以SDS-PAGE分析部分純化之海鱺血清蛋白質分解酵素抑制物,A組在非還原狀態於185KDa附近出現一條蛋白帶,而在還原狀態於88KDa附近出現一條主要的蛋白帶。B組在非還原狀態下於74KDa出現一條蛋白帶,而在還原狀態下則於77KDa及62KDa出現兩條蛋白帶。
  此兩種部分純化的海鱺血清蛋白質分解酵素抑制物在經60℃處理二十分鐘後,其抑制蛋白質分解酵素活性皆僅餘10﹪,為熱不安定型之酵素。由於以0.2M methylamine處理30分鐘後,其抑制蛋白質分解酵素抑制活性明顯地受到影響,以及其等電點值約在pI 4.15~4.55之間,因此推測海鱺血清中的蛋白質分解酵素可能為α2-macroglobulin的相似物。
中文摘要------------------------------------------------------Ⅰ
英文摘要------------------------------------------------------Ⅲ
表目錄--------------------------------------------------------Ⅴ
圖目錄--------------------------------------------------------Ⅴ
一、前言-------------------------------------------------------1
二、文獻整理---------------------------------------------------5
三、材料方法--------------------------------------------------14
 3.1海鱺血清之取得-------------------------------------------14
  3.1.1海鱺(Rachycentron canadum)來源---------------------14
  3.1.2海鱺血清之取得與保存---------------------------------14
 3.2弧菌及其細胞外產物---------------------------------------15
  3.2.1菌株來源及保存---------------------------------------15
3.2.2細菌細胞外產物之製備及保存---------------------------15
3.3海鱺血清中蛋白分解酵素抑制物之分離純化-------------------16
3.3.1硫酸銨沈澱法(Ammonium sulfate precipitation)-------16
3.3.2疏水性管柱層析法(Hydrophobic interaction
chromatography, HIC)--------------------------------17
3.3.3親和力層析法(Affinity chromatography)--------------17
3.3.4 陰離子交換法─RESOURCE Q(Pharmacia)管柱層析法-----18
3.3.5 陰離子交換法─MONO Q(Pharmacia)管柱層析法---------18
3.4蛋白分解酵素抑制物純度(Homogeneity)之鑑定及分子量
(Molecular weight)之估算--------------------------------18
3.4.1電泳及染色-------------------------------------------18
3.4.2海鱺血清中蛋白質分解酵素抑制物質之鑑定---------------20
3.4.3交叉免疫電泳(Crossed immunoelectrophoresis)--------20
3.5兔子抗海鱺全血清抗血清之製備-----------------------------21
3.6部分純化之海鱺血清蛋白質分解酵素抑制物之特性分析---------22
3.6.1蛋白質含量之測定-------------------------------------22
3.6.2血清蛋白質分解酵素抑制物之抑制活性測定---------------22
3.6.3等電點(isoelectric point,pI)之判定----------------23
3.6.4Methylamine對海鱺血清蛋白酵素抑制物抑制細菌細胞外產
物蛋白分解酵素活性之影響-----------------------------24
3.6.5溫度對海鱺血清蛋白分解酵素抑制物抑制活性之影響-------24
3.6.6海鱺血清蛋白分解酵素抑制物對不同稀釋度之細菌細胞外產
物的抑制活性-----------------------------------------24
四、結果------------------------------------------------------25
4.1海鱺血清蛋白分解酵素抑制物之分離純化---------------------21
4.2蛋白質分解酵素抑制物質之純度鑑定及分子量估算-------------27
4.3交叉免疫電泳分析海鱺部分純化之蛋白分解酵素抑制物質-------28
4.4海鱺血清部分純化蛋白質分解酵素抑制物之等電點值(pI value)
---------------------------------------------------------28
4.5Methylamine對部分純化海鱺血清蛋白質分解酵素抑制物抑制活
性之影響-------------------------------------------------29
4.6溫度對部分純化之海鱺血清蛋白質分解酵素抑制物抑制活性之影響
---------------------------------------------------------29
4.7部分純化之海鱺血清蛋白分解酵素抑制物對不同稀釋度之細菌細
胞外產物的抑制活性---------------------------------------30
五、討論------------------------------------------------------32
六、參考文獻--------------------------------------------------39
表------------------------------------------------------------48
圖------------------------------------------------------------49
古鎮均、陳秀男,2001。台灣海鱺繁、養殖技術現況與評析。海鱺箱網養殖發展研討會,39-49。
李國誥、劉秉忠、郭光雄、陳秀男,1997。草蝦弧菌Vibrio harveyi 770527之主要外毒素研究。農委會漁業特刊第六十一號,魚病研究專集,18:33-42。
李國誥、劉秉忠、陳秀男,1996。草蝦弧菌致病因子免疫性研究。農委會漁業特刊第五十四號,魚病研究專集,17:1-13。
邵廣昭、陳正平、沈士傑,1992。墾丁國家公園海域魚類圖鑑。內政部營建屬墾丁國家公園管理處出版,427。
陸知慧,2000。箱網養殖海鱺魚類疾病調查與防制。行政院農業委員會漁業署九十年度試驗研究計畫報告,56-62。
楊三品,2000。海鱺種苗生長概況。研商海鱺養殖發展方向座談會會議記錄。農委會漁業署。
劉秉忠,1996。哈威弧菌對草蝦之致病機制研究。國立台灣大學動物學研究所,博士學位論文。陳逢叡,1999。溶藻弧菌蛋白分解酵素特性及其對斑節蝦之致病機制研究。國立台灣海洋大學水產養殖係研究所,博士學位論文。林積陽,2002。巴斯德桿菌對養殖海鱺之致病機制研究。國立台灣海洋大學水產養殖係研究所,碩士學位論文。
蘇偉銘,1999。病原性弧菌其細胞外產物與石班魚血清成分之交互作用。國立台灣海洋大學水產養殖系研究所,碩士學位論文。李正皓,2001。養殖石班魚血清中弧菌蛋白質分解酵素抑制物之研究。國立台灣海洋大學水產養殖系研究所,碩士學位論文。
Abrams, W. R., Kimbel, P. and Weinbaum, G., 1978. Purification and characterization of canine α1-antiproteinase. Biochemistry. 17: 3556-3561.
Alexander, J. B. and Ingram, G. A., 1992. Noncellular nonspecific defence mechanisms of fish. Ann. Rev. Fish Dis., 2: 249-279.
Alexander, J. B., 1985. Non-immunoglobulin humoral defence mechanism in fish. In: Fish Immunology. Ed. Manning, M. J., Tatner, M. pp. 133-140. Academic Press, London.
Andus, T., Gross, V., Northemann, W., Tran-Thi, T-A. and Heinrich, P. C.,1983. Rat plasma proteinase inhibitors: the biosynthesis of α2-macroglobulibn. In: proteinase inhibitors: Medical and Biological Aspects. Ed. Katunuma, N., Umezawa, H. and Holzer, H., pp. 243-250. Japan Sci. Soc. Press, Tokyo.
Aranishi, F., 1999. Purification and characterization of α1-proteinase inhibitor from carp (Cyprinus carpio) serum. Mar. Biotechnol., 1: 33-43.
Austin, B. and Austin, D. (eds.), 1993. Bacterial Fish Pathogens: Disease in Farmed and Wild Fish, 2nd ed. Ellis Horwood Ltd, Chichester.
Balebona, M. C., Morinigo, M.A., Sedano, J., Martinez-Manzanares, E., Vidaurreta, A., Borrego, J. J. and Toranzo, A. E., 1992. Isolation of Pasteurella piscicida from sea bass in Southwestern Spain. Bull. Eur. Ass. Fish Pathol., 12: 168-170.
Barrett, A. J., 1980. Protein degradation in health and disease. In : Cuba Found. Symp. 75. Ed. Evered, D., and Whelan, J. pp 1-13. Excerpta Medica, Amsterdam.
Baskerville, A., Conlan, J. W., Ashworth, L. A. E. and Dowsett, A. B., 1986. Pulmonary damage caused by a protease from Legionella pneumophlia. Brit. J. Experi. Pathol., 67: 527-536.
Bejarno, P. A., Langeveld, J. P. M., Hudson, B. G. and Noelken, M. E., 1989. Degradation of basement membranes by Pseudomonas aeruginosa elastase. Infect. Immun., 57: 3783-3787.
Berninger, R. W. and Mathis, R. K., 1976. Isolation and characterization of α-1-antitrypsin from rhesus-monkey serum. Biochem. J., 159: 95-104.
Björck, L. and Grubb, A.,Kjellen, L., 1990. Cystatin C, a human proteinase inhibitor, block replication of Herpes simplex virus. J. Virol., 64: 941-943.
Björklund, H. V., Johansson, T. R. and Rinne, A., 1997. Rhabdovirus-induced apoptosis in a fish cell line is inhibited by human endogenous acid cysteine proteinase inhibitor. J. Virol., 71: 5658-5662.
Blackwood, L. L., Stone, R. M. Iglewski, B. H. and Pennington, J. E., 1983. Evaluation of Pseudomonas aeruginosa exotoxin A and elastase as virulence factors in acute lung infection. Infect. Immun., 39: 198-201.
Blankenvoorde, M. F., van’t Hof, W., Walgreen-Weterings, E., van Steenbergen, T, J., Brand, H, S., Veerman, E. C. and Nieuw Ameronogen, A. V., 1998. Cystatin and cystatin-derived peptides have antibacterial activity against the pathogen Porphyromonas gingivalis. Biol. Chem., 379: 1371-1375.
Bly, J. E. and Clem, W. L., 1992. Temperature and teleost immune function. Fish Shellfish Immunol., 2: 159-171.
Bowden, T. J., Bulter, R., Bricknell, I. R. and Ellis, A. E., 1997. Serum trypsin-inhibitory activity in five species of five farmed fish. Fish Shellfish Immunol., 7: 377-385.
Cao, M-J., Osatomi, K., Matsuda, R., Ohkubo, M., Hara, K. and Ishihara, T., 2000. Purification of a novel proteinase inhibitor from the skeletal muscle of white croaker (Argyrosomus argentatus). Biochem. Biophy. Res. Commun., 272: 485-489.
Carrell, R. W. and Boswell, D. R., 1986. Serpins: the superfamily of plasma serine proteinase inhibitors. In: Reasearch Monographs in Cell and Tissue Physiology. Ed. Barrett, A. J. and Salvessen, G., 12: 403-420. Elservier, Amsterdam.
Carrell, R. W. and Travis, J., 1985. α1-antitrypsin and the serpins: variation and countervariation. Trends Biochem. Sci., 10: 20-24.
Carrell, R. W., 1984. Therapy by instant evolution. Nature, 312: 14.
Carrell, R. W., Pemberton, P. A. and Boswell, D. R., 1987. The serpins: evolution and adaptation in a family of protease inhibitors. Cold Spring Habor Symp. Quant. Biol., 52: 527-535.
Ciereszko, A., Piros, B., Dabrowski, K., Kucharczyk, D., Luczynski, M. J., Dobosz, S. and Glogowski, J., 1998. Serine proteinase inhibitors of seminal plamsa of teleost fish: distribution of activity, electrophoretic proflies and relation to proteinase inhibitors of blood. J. Fish Biol., 53: 1292-1305.
Collins, A. R. and Grubb, A., 1998. Cystatin D, a natural salivary cysteine proteinase inhibitor, inhibits coronavirus replication at its physiological concentration. Oral Microbiol. Immunol., 13: 59-61.
Conlan, J. W., baskerville, A. and Ashworth, L. A. E., 1986. Separation of Legionlla pneumophila proteases and purification of a protease which produce lesions like those of legionnaires’ disease in guinea-pig lung. J. Gen. Microbiol., 132: 1565-1574.
Corbel, M. J., 1975. The immune response in fish: A review. J. Fsih Biol., 7: 539-563.
Dabrowski, K. and Ciereszko, A., 1994. Anti-proteinase inhibitor(s) in fish seminal plasma of teleost fish. J. Fish Biol., 45: 801-809.
De la cruz, M. C. and Muroga, K., 1989. The effect of Vibrio anguillarum extracellular products on iapanese eels. Aquaculture, 80: 201-210.
Diven, W. K., Vietmeier, B., Hempel, J. and Chambers, J., 1990. Purification and N-terminal characterization of chinchilla villidera α1-antitrypsin. Comp. Biochem. Physiol., 95B: 39-44.
Egidius, E., 1987. Vibriosis: Pathogenicity and pathology. A review. Aquaculture, 67: 15-28.
Eills. A. E., 1987. Inhibition of the Aeromnoas salmonicida extracellular protease by α2-macroglobulin in the serum of rainbow trout. Micro. Pathog., 3: 167-177.
Ellis, A. E. (ed), 1989. The immunology of teleosts. In: Fish Pathology. 2nd. Ed. Robert, R. J.pp. 135-152.
Ellis, A. E. and Grisley, M. S., 1985. Serum anti-proteases of salmonids: studies oof the inhibition of trypsin and the proteolytic activity of Aeromonas salmonicida extracellular products. In: Fish and Shellfish Pathology. Ed. Eliis, A. E. pp. 85-96. Academic Press, London.
Ellis, A. E., 1981. Non-specific defense mechanisms in fish and their role in disease processes. Dev. Biol. Standard., 49: 337-352.
Ellis, A. E., 1990. Serum antiproteases in fish. In: Techniques in Fish Immumology Ed. Stolen, J. S., Fletsher, T. C., Anderson, D.P., Roberson, B. S. and van Muiswinkel, W. B. pp. 95-99. SOS Publications, Fair Haven, New Jeresy.
Hara, K., and Ishihara, T., 1987. Purification and characterization of serine proteinase inhibitor from carp Cyprinus carpio ordinary muscle. Agric. Biol. Chem., 51: 153-159
Hara, K., Nakaoka, H., Nosaki, Y., Tabata, Y. and Ishhara, T.,1985. Purification and characterizaiton of serine protease inhibitor from white croaker Argyrosomus argentatus ordinary muscle. Nippon Suisan Gakkaishi, 51: 153-159.Hare, P. T., Scott B. T. and Wood, D.R., 1983. Characterization of extracellular alkaline proteases and collagenase induction in Vibrio alginolyticus. J. Gen. Microbiol., 129: 1141-1147.
Hercz, A., 1982. Isolation and characterization of goat serum α1-globulin protease inhibitors. Can. J. Biochem., 60: 28-35.
Hjeltnes, K., 1983. Proteinase inhibitors in the muscle and serum of cod (Gadus morhua). Isolation and characterization. Comp. Biochem. Physiol., 76B: 365-372.
Holder, I. A. and Haidaris, C. G., 1979. Experimental studies of the pathogenesis of infections due to Pseudomonas aeruginosa: extracellular protease and elastase as in vivo virulence factors. Can. J. Microbiol., 25: 593-599.
Honn, K. V., Cavanaugh, P., Evens, C. and Taylor, J. D., 1982. Tumor cell-platelet aggregation : induced by cathepsin B-like proteinase and inhibited by prosacyclin. Science, 217: 540-542.
Howe, T. R. and Iglewski, B. H., 1984. Isolation and characterization of alkaline protease-deficient mutants of Pseudomonas aeruginosa in vitro and in a mouse eye model. Infect. Immun., 43: 1058-1063.
Huang, C. J., Chen, C. C., Chen, H. J., Huang, F. L. and Chang. G. D.,1995. A protease inhibitor of the serpin family is a major protein in carp perimeningeal fliud. I. Protein purification and characterization. J. Neurochem., 64: 1715-1720.
Ingram, G. A., 1980. Substance involved in natural resitance of fish to infection A review. J. Fish Biol., 16: 23-60.
Irie, K., Hosoyama, H., Takeuchi, T., Iwabuchi, K., Watanabe, H., Abe, M., Abe, K. and Arai, S., 1996. Transgenic rice established to express corn cystatin exhibits strong inhibitory activity against insect gut proteinases. Plant Mol. Biol., 30: 149-157.
Kobayashi, S. and Nagasawa, S., 1974. Protease inhibitors in guinea pig serum, 1. isolation of two functoinally different teypsin inhibit from guniea pig serum. Biochem. Biophys. Acta., 342: 372-381.
Koj, A., Hatton, M. W. C., Wong, K. L. and Regoeczi, E., 1978. Isolation and partial characterization of rabbit plasma α1-antitrypsin. Biochem. J., 169: 589-596.
Kothary, M. H. and Kreger, A. S., 1985. Production and partial characterization of an elastolyitc protease of Vibrio vulnificus. Infect. Immun., 50: 534-540.
Kothary, M. H. and Kreger, A. S., 1987. Purification and characterization of an elastolyitc protease of Vibrio vulnificus. J. Gen. Microbiol., 133: 1783-1791.
Kusuda, R. and Yamaoka, M., 1972. Etiological studies of bacterial pesudotuberculosis in cultured yellowtail with Pasteurella piscicida as the causative agent. I. On the morphological and biochemical properties. Bull. Jpn. Soc. Sci. Fish., 38: 1325-1332.Lee, K. K., 1995. Pathogenesis studies on Vibrio alginolyticus in the grouper, Epinephelus malabaricus, Bloch et Schneider. Microbiol. Pathog., 19: 39-48.
Lee, K. K. and Ellis, A. E., 1991. Interaction between salmonid serum components and the extracellular hemolytic toxin of Aeromonas salmonicida. Dis. Aquat. Org., 11: 207-216.
Lee, K. K. and Ellis, A. E., 1991. The role of the lethal extracellular cytolysin of Aeromonas salmonicida in the pathology of furunculosis. J. Fish. Dis., 14: 453-460.
Lee, K. K., Yu, S, R., Yang, T. I., Liu, P. C. and Chen. F. R., 1996a. Isolation and characterization of Vibrio alginolyticus isolated from diseased kuruma prawn, Penaeus japonicus. Lett. Appl. Microbiol., 22: 111-114.
Lee, K. K., Yu, S. R. and Liu, P.C., 1997. Alkaline seine protease is an exotoxin of Vibrio alginolyticus in kuruma prawn, Penaeus japonicus. Curr. Microbiol., 34: 110-117.
Lee, K. K., Yu. S R., Chen. F. R., Yang, T. I. And Liu. P. C., 1996b. Virulence of Vibrio alginolyticus isolated from diseased tiger prawn, Peaneus monodon. Curr. Microbiol., 32: 229-231.Liu, P. C. and Lee, K.K., 1999. Cysteine protease is a major exotoxin of pathogenic luminous Vibrio harveyi in the tiger prawn , Penaeus monodon. Lett. Appl. Microbiol., 28: 428-430.
Magarinons, B., Romalde, J. L., Bandin, I., Fouz, B. and Toranzo, A. E., 1992. Phenotypic, antigen and molecular characterization of Pasteuralla piscicida strains isolated from fish. J. Appl. Microbiol., 58: 3316-3322.
Miyoshi, N., Miyoshi, S., Sugiyama, K., Suzuki, Y., Furuta, H and Shinoda, S., 1987b. Activation of the plasma kallikrein-kinin system by Vibrio Vulnificus protease. Infect. Immun., 55: 1936-1939.
Miyoshi, N., Shimizu, C., Miyoshi, S. and Shinoda, S., 1987a. Purification and characterization of Vibrio Vulnificus protease. Micriobiol. Immunol., 31: 13-25.
Munro, A. L. S., Hastings, T. S., Ellis, A. E. and Liversidge, J., 1980. Studies on an ichthyotoxic material produced extracellular by the furunculosis bacterium Aermonas salmonicida. In : Fish Diseases. Ed. Ahne, W. pp 98-106. Springer-Verlag, Berlin.
Neurath, H., 1984. Evolution of proteolytic enzymes. Science, 224: 350-357.Nottage, A. S., and Birkbeck, T. H., 1986. Toxicity of marine bivalves of culture supernatant fluids of the bivalve-pathogenic Vibrio strain NCMB 1338 and other marine vibrios. J. Fish. Dis., 9: 249-256.Nottage, A. S. and Birkbeck, T. H., 1987a. Purification of a proteinase produced by the bivalve pathogen Vibrio alginolyticus NCMB 1339. J. Fish. Dis., 10: 211-220.Nottage, A. S. and Birkbeck, T. H., 1987b. Producing of proteinase during experimental infection of Ostrea edulis L. larvae with Vibrio alginolyticus NCMB 1339 and the antigenic relationship between proteinase produced by marine vibrios pathogenic for fish and shellfish. J. Fish. Dis., 10: 265-273.Osatomi, K., Sasai, H., Cao, M., Hara, K. and Ishihara, T., 1997. Purification and characterization of myofibril-bound serine proteinase from carp Cyprinus carpio ordinary muscle. Comp. Biochem. Physiol., 116B: 183-190.
Patterson, S. D., 1991. Mammalian α1-antitrypsins: comparative biochemistry and genetics of the major plasma serpins. Comp. Biochem. Physiol., 100B: 439-454.
Patterson, S. D., Bell, K. and Poole, W. E., 1987. Tammer wallaby plasma protease inhibitory (Pi) proteins. Aust. J. Biol. Sci., 40: 355-364.
Pavlovskis, O. R. and Wretlind, B., 1979. Assessment of protease (elastase) as a Pesudomonas aeruginosa virulence factor in experimental mouse burn infection. Infect. Immun., 24: 181-187.
Pierre, P. and Mellman, I., 1998. Development regulation of invariant chain proteolysis controls MHC class II trafficking in mouse dendritic cells. Cell, 93: 1135-1145.
Rajan, JPR., Lopez, C., Lin, JH-Y. and Yang, H. L., 2001. Vibriosis in culture cobia, Rachycentron canadum L., in Taiwan. 6 th Asian Fisheries Forum Book of Abstracts, Asian Fisheries Society, Unit A, p.208. Mayaman Townhomes 25 Mayaman Street UP Village, Quezon City Philippines.
Rijkers, G. T., 1982. Kinetics of humoral and cellular immune reactions in fish. Dev. Comp. Immunol. Suppol., 2: 93-100.
Rinne, A., Alavaikko, M., Järvinen, M., Martikainen, J., Karttunen, T. and Hopsu-Havu, V., 1983. Demonstration of immunoreaction acid cysteine-proteinase inhibitor in reticulum cells of lymph node germinal centres. Vichows Arch., B 43: 121-126.
Roll, D. E. and Glew, R. H., 1981. Isolation and characterization of rat α-1-antitrypsin. J. Biol. Chem., 256: 8190-8196.
Rosenberg, M., Roegner, V. and Becker, F., 1976. Isolation and characterization of two α1-protease inhibitors in rat serum. Am. Rev. Dis., 113: 779-785.
Salte, R., Norberg, K. and Odegaard, O. R., 1995. Some functional properties of teleost anti-thrombin. Thromb. Res., 83: 389-397.
Salte, R., Norberg, K. and Odegaard, O. R., 1996. Evidence of a protein C anticoagulant system in bony fishes. Thromob. Res., 80: 193-200.
Santos, Y., Bandin, I., Nunez, S., Montero, M., Silva, A. and Toranzo, A. E., 1992. Comparison of the extracellular biological activities of Vibrio anguillarium and Aeromonas hydrophila. Aquaculture, 107: 259-270.
Smith, G. C. and Merkel, J. R., 1982. Collagenolytic activity of Vibrio vulnificus : potential contribution to its invasiveness. Infect. Immun., 35: 1155-1156.Sniezsko, S. F., Bullock, G. L., Hollis, E. and Boone, J. G., 1964. Pasteurella sp. From an epizootic of white perch (Roccus americanus) in Chesapeak Bay tidewater areas. J. Bacteriol., 88: 1814-1815.
Starkey, P. M., Fletcher, T. C. and Barrett, A. J., 1982. Evolution of α2-macroglobulin. The purification and characterization of a protein homologous with human α2-macroglobulin from plaice (Pleuronectes platessa L.) plasma. Biochem. J., 205: 97-104.
Takahara, H. and Sinohara, H., 1982. Mouse plasma trypsin inhibitors. J. Biol. Chem., 257: 2438-2446.
Tanter, M. F., 1996. Natural changes in the immune system of fish. In: The Fish Immune System. Ed. Iwama, G. and Nakanishi, T., pp. 255-288. Academic Press.
Thune, R. L., Stanley, L. A. and Copper, R. k., 1993. Pathogenesis of Gram-negative bacterial infections in warmwater fish. Ann. Rev. Fish Dis., 3: 37-68.
Toranzo, A. E. and Barja, J. L., 1993. Virulence factors of bacterial pathogenic for clodwater fish. Ann. Rev. Fish Dis., 3; 25-26.
Travis, J. and Johnson, D., 1981. Human α1-proteinase inhibitor. Methods Enzymol., 80: 754-765.
Travis, J. and Salvesen, G. S., 1983. Human plasma proteinase inhibitors. Ann. Rev. Biochem., 52: 655-709.
Vanghan, L., Lorier, M. A. and Carrell, R. W., 1982. α1-antitrypsin microheterogeneity: isolation and vnhysiological significance of isoforms. Biophys. Acta., 701: 339-345.
Westrom, B. R., 1979. Identification and characterization of trypsin, chymotrypsin and elastase inhibitors in procine serum. Hoppe-Seyler'' S Z Physiol Chem., 360: 1861-1878.
Woods, D. E., Cryz, S. J., Friedman, R. L. and Iglewski, B. H., 1982. Contribution of toxin A and elastase to virulence of Pseudomonas aeruginosa in chronic lung infection of rats. Infect. Immun., 36: 1223-1228.
Zuo, X. and Woo, P.T.K., 1997. Natural anti-proteases in rainbow trout, Oncorhynchus mykiss and brook charr, Salvelinus fontinalis and the in vitro neutralization of fish α2-macroglobulin by the metalloprotease from the pathogenic haemoflagellate, Cryptobia salmositica. Parasitology, 114: 375-381.
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top