臺灣博碩士論文加值系統

本研究為探討吳郭魚稚魚(Oreochromis niloticus × O. aureus)與石斑魚稚魚(Epinephilus marabaricus)之維生素B1最適需求量。吳郭魚稚魚(平均初重0.63  0.01 g)給予八組不同維生素B1含量(0, 0.5, 1.0, 2.0, 3.0, 4.0, 8.0, 16.0 mg/kg diet)之飼料，每組三重複，共飼養八週。結果顯示餵食未添加維生素B1組之吳郭魚稚魚其魚體增重百分率、飼料效率及蛋白質利用率皆顯著(P<0.05)低於其他餵食含有維生素B1之各組。吳郭魚血液、肝臟及肌肉中維生素B1含量與血液及肝臟transketolase(TK)活性皆以餵食未添加維生素B1組為最低；而thiamin pyrophosphate(TPP) effect值則以此組為最高。魚體增重百分率、血液中維生素B1含量及血液中TK活性以broken-line迴歸分析求得吳郭魚稚魚對維生素B1之最適需求量為1.4-2.1 mg/kg diet (研究一)。石斑魚稚魚(平均初重4.09  0.08 g)給予八組不同維生素B1含量(0, 0.5, 1.0, 2.0, 3.0, 6.0, 10.0, 20.0 mg/kg diet)之飼料，每組三重複，共飼養八週。結果顯示餵食維生素B1缺乏組之存活率、魚體增重率、飼料效率及蛋白質利用率等均較其他餵食添加維生素B1組為差。餵食未添加維生素B1組之血液、肝臟及肌肉中之維生素B1含量以及血液和肝臟中TK活性皆較餵食B1組為低；而其TPP-effect值則較高。當魚體增重百分率、血液中維生素B1含量及血液中TK活性以broken-line迴歸分析獲得石斑魚稚魚之維生素B1最適需求量為0.7-1.0 mg/kg diet (研究二)。

The study was aimed at quantifying the optimal vitamin B1 (B1) requirements of juvenile hybrid tilapia (Oreochromis niloticus  O. aureus) and grouper (Epinephilus malabaricus). In Experiment I, purified diets with 8 levels (0, 0.5, 1.0, 2.0, 3.0, 4.0, 8.0, 16.0 mg/kg diet) of supplemental vitamin B1 were each fed to triplicate groups of tilapia (mean initial body weight 0.63  0.01 g) for 8 weeks. Results indicated that fish fed the unsupplemented vitamin B1 control diet had lower (P<0.05) weight gain (WG), feed efficiency (FE), protein efficiency ratio (PER) and vitamin B1 concentration in tissues than fish fed diets supplemented with B1. Hepatic and blood transketolase (TK) activity increased, while thiamin pyrophosphate (TPP) effect decreased in fish as the dietary B1 supplementation level increased. Analysis of the WG, blood B1 concentration and TK activity of the fish by broken-line regression indicate that the optimal dietary vitamin B1 requirement for tilapia is approximately 1.4-2.1 mg/kg diet. In Experiment II, purified diets with 8 levels (0, 0.5, 1.0, 2.0, 3.0, 6.0, 10.0, 20.0 mg/kg diet) of supplemental B1 were each fed to triplicate groups of grouper (mean initial body weight 4.09  0.08 g) for 8 weeks. Results indicated that fish fed the B1 unsupplemented control diet had lower WG, FE, PER and tissue vitamin B1 concentration than fish fed diets supplemented with B1. Analysis of the WG, blood B1 concentration and TK activity of the fish by broken-line regression indicate that the optimal dietary vitamin B1 requirement for grouper is approximately 0.7-1.0 mg/kg diet.

總摘要（中文） ּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּ I
文獻整理 ּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּ 1
研究一：吳郭魚稚魚對維生素B1最適需求量之探討
摘要 ּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּ 19
前言 ּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּ 20
材料與方法 ּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּ 22
結果 ּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּ 37
討論 ּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּ 48
研究二：石斑魚稚魚對維生素B1最適需求量之探討
摘要 ּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּ 51
前言 ּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּ 52
材料與方法 ּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּ 53
結果 ּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּ 68
討論 ּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּ 78
總結論 ּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּ 81
參考文獻 ּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּּ 82

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